Anita Olejek

Medical University of Silesia in Katowice, Catowice, Silesian Voivodeship, Poland

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Publications (96)63.68 Total impact

  • E. Podwińska · I. Janosz · P. Bodzek · A. Brenk · A. Olejek
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    ABSTRACT: Introduction. In the recent years, it has been observed that Cesarean sections tend to be conducted more and more frequently in departments of obstetrics. The development of medicine has contributed to the increase in the safety of this procedure. However, complications associated with Cesarean sections are still more common than those after natural labors. Aim. Learning about the opinions of women concerning labor and the possibilities of delivery as well as the role of an anesthesiologist during the procedure of a Cesarean section. Material and methods. A survey was conducted on a group of 200 patients of a delivery ward and fetal medicine unit. Results. Most respondents (64%) believe that pain is a natural element of labor, and 23.5% of women claim that it is the feeling that should be acted against. A Cesarean section is considered safer for a newborn by 51% of women vs 21% who claim that natural childbirth is safer. 65.5% of patients believe that a Cesarean section on request is acceptable. Conclusions. A Cesarean section, which is wrongly perceived as safer for a newborn by most women, carries a number of risks for a child. It is necessary to educate patients more intensively concerning the methods of relieving pain during labor, particularly in antenatal classes.
    No preview · Article · Sep 2015
  • A. Bakunowicz · A. Brenk · A. Olejek
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    ABSTRACT: Summary Introduction. Infertility is the lack of possibility of producing offspring, although regular sexual intercourse without use of contraceptives are present for over a year. It is a disease affecting around a million of couples in Poland. Aim of the work. The analysis of social, demographic and mental factors and the presentation of causes of infertility in Poland. Material and methods. 250 infertile women were questioned through one of the subject-related web portals. Results. The married women constituted the vast majority of the group (94.9%), so did the women currently working (86.5%). Approximately 1/3 of the respondents were overweight Almost 80% attempted to get pregnant for a period from a year to 5 years. Half of the respondents has started their attempts at getting pregnant when they were aged 25 to 30. Over 90% of the respondents declare the will to use the in vitro procedure. Among the causes the most frequent ones are hormonal disorders, ovulation disorders and PCOS. Patients with infertility problems were anxious, feared and depressed, those feelings worsened with the prolonging time of their attempts to get pregnant. Conclusions. The problem of infertility affects mostly the women with stabilized material and professional situation and higher education. With the prolonging infertility negative feelings and emotions appear, such as: anxiety, despondence, frustration, fear, stress that lead to the ever worsening family situation and disturb the social functioning. In the opinion of the respondents the in vitro procedure is an effective way for fighting infertility and a chance to have offspring. Hormonal disturbances, disturbances of ovulation, endometriosis, the obstructed oviducts and anatomical malformations of uterus were the most common causes of infertility in case of the researched group of women.
    No preview · Article · Jan 2015
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    ABSTRACT: Vulvar cancer accounts for about 3-5% of all female genital carcinomas. TGF-P protein is a member of a superfamily of cytokines that regulate cell functions. A correlation between this protein and many neoplastic processes was reported. In our study we analyzed TGF-β expression in vulvar tumor among patients with diagnosed squamous cell carcinoma (with and without inguinal nodes metastases). Paraffin embedded blocks obtained from vulvar tissues and inguinal nodes (from 31 patients with vulvar carcinoma FIGO ll-IV) were prepared. Next, the hematoxylin and eosin staining was performed. Monoclonal antibody NCL-TGF-beta was used for immunohistochemical tests. Higher expression of TGF-beta in cancer cells corresponds to more advanced cancer stages (FIGO). A positive correlation between TGF-beta and metastases, as well as a number of inguinal nodes metastases was observed. The ratio between the number of stained cells in vulvar tumor and of inflammatory cells proved to be higher in FIGO stage III than IV Possibly TGF-beta increase in vulvar tumor contributes to the breakdown of immunological processes limiting cancer progression. Higher TGF-beta expression leads to metastasis in regional lymphatic nodes. TGF-beta overproduction is observed in vulvar neoplastic processes. In early stages of carcinogenesis TGF-beta inhibits cancer cell proliferation, but in more advanced stages it accelerates cancer progression by inhibiting the immunological response.
    No preview · Article · Nov 2014 · Ginekologia polska
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    ABSTRACT: The study aimed at investigating the impact of late prematurity (LPT) on neonatal outcome in twins and neonatal morbidity and mortality within LPT with regard to the completed weeks of gestation. The study was conducted in six tertiary obstetric departments from different provinces of Poland (Warsaw, Lublin, Poznan, Wroclaw, Bytom). It included 465 twin deliveries in the above centers in 2012. A comparative analysis of maternal factors, the course of pregnancy and delivery and neonatal outcome between LPT (34 + 0-36 + 6 weeks of gestation) and term groups (completed 37 weeks) was performed. The neonatal outcome included short-term morbidities. The analysis of neonatal complication rates according to completed gestational weeks was carried out. Out of 465 twin deliveries 213 (44.8%) were LPT and 156 (33.55%) were term. There were no neonatal deaths among LPT and term twins. One-third of LPT newborns suffered from respiratory disorders or required antibiotics, 40% had jaundice requiring phototherapy, and 30% were admitted to NICU. The analysis of neonatal morbidity with regard to each gestational week at delivery showed that most analyzed complications occurred less frequently with the advancing gestational age, especially respiratory disorders and NICU admissions. The only two factors with significant influence on neonatal morbidity rate were neonatal birth weight (OR = 0.43, 95% CI = 0.2-0.9, p = .02) and gestational age at delivery (OR = 0.62, 95% CI = 0.5-0.8, p < .01). LPT have a higher risk of neonatal morbidity than term twins. Gestational age and neonatal birth weight seem to play a crucial role in neonatal outcome in twins.
    Full-text · Article · Aug 2014 · Twin Research and Human Genetics
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    Iwona Gabriel · Anita Olejek
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    ABSTRACT: Women using hormonal therapy for breast cancer are often encountered in clinical practice of breast surgeons, oncologists and gynecologists. Some of them during the course of therapy develop abnormal uterine bleeding or have ultrasound abnormalities detected. In Poland, most of them are still diagnosed using blind curettage, which does not bring a definitive diagnosis or requires repeating the diagnostic procedure. This produces unnecessary fear of malignancy in patients and increases economic costs (double curettage, unnecessary hysterectomy and its social consequences). Therefore, we studied the usefulness of hysteroscopy with targeted biopsies for further management of women treated with tamoxifen. Our goal is to provide evidence that women before entering, during and after tamoxifen treatment have hysteroscopy for endometrial assessment.
    Preview · Article · Jun 2014 · Videosurgery and Other Miniinvasive Techniques / Wideochirurgia i Inne Techniki Malo Inwazyjne
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    ABSTRACT: The aim of the study was to determine a relation between the level of total serum bile acids in pregnant women and the condition of their newborns on the basis of select parameters of acid-base balance in the cord blood. The study group consisted of 64 pregnant women with Intrahepatic Cholestasis of Pregnancy and 78 healthy pregnant women constituted the control group. Colorimetric enzymatic measurements of serum total bile acids were performed in both groups. In the mild cholestasis group, mean concentration of bile acids amounted to 20.6 +/- 1.0 micromol/l and was statistically significantly lower as compared to women with severe cholestasis (58.9 +/- 9.5 micromol/l). In the control group, mean concentration of bile acids was 4.1 +/- 0.3 micromol/l and was statistically significantly lower as compared to groups with cholestasis. Caesarean section was performed in 60.9% of women with cholestasis as compared to 19.2% of controls. Also, neonatal condition in the first minute of life was worse in the group with cholestasis as compared to the infants in the control group. The body weight of newborns of mothers with cholestasis was statistically significantly lower than in infants born to controls. Lowered neonatal pH (below 7.20) was noted in 2.6% of the newborns from the control group and was statistically significantly less frequent as compared to infants from the group with cholestasis. Periodic measurements of total bile acid concentrations in serum of pregnant women with cholestasis is of high value in establishing the diagnosis and determining severity of the disease in the mother as well as potential hazard for the fetus. Simultaneous monitoring of the changes total bile acids concentrations in the blood serum and the well-being allows to reduce the rates of neonatal distresses after birth and cesarean sections.
    No preview · Article · Feb 2014 · Ginekologia polska
  • A. Olejek · I. Gabriel · B. Mazur
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    ABSTRACT: Introduction. CD4+ T helper lymphocytes may be divided functionally into Th1, Th2, Treg and Th17 lymphocytes. The latter type is a subpopulation of T lymphocytes producing IL-17, IL0-17F, IL-21 and IL-22. Recently, new supplemented trends have been suggested for the understanding of the immunologic basis of miscarriage, where it was demonstrated that patients with recurrent miscarriages displayed an elevated number of Th17 lymphocytes in circulating blood and decidua as compared to women with physiological pregnancy. Aim of the study. The aim of the study was an assessment of the percentage share of Th17 lymphocytes in inducing mscarriage in the first trimester of pregnancy among women in the Silesian urban agglomeration. Material and methods. The study covered 52 women with miscarriage in the first trimester of pregnancy. The collected blood samples were subjected to fluorocytometric analysis at the Laboratory of Flow Cytometry of the Independent Public Clinical Hospital No.1 in Zabrze (Poland). All the collected blood samples underwent cytometric analysis. Results. In women with miscarriage in the first trimester of gestation the mean rate of T lymphocytes was 27.19%, the mean rate of CD4+ T lymphocytes was 10.52%, and the mean rate of IL-17-producing T lymphocytes was 2.69%. Conclusions. The authors' own studiy suggests that CD4+ T helper lymphocytes producing IL-17 may be activated during spontaneous miscarriage. The study was the first one conducted in the Silesian urban agglomeration that assessed the mobilization of Th17 lymphocytes in the course of spontaneous miscarriage.
    No preview · Article · Jan 2014
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    ABSTRACT: Material and methods. The study included 64 women: 28 pregnant women with pregnancy complicated by gestational hypertension, 16 healthy pregnant women and 20 healthy women who are not pregnant. The designation of the concentrations of heat shock proteins having a molecular weight of 60 kDa (Hsp60) was performed with use of enzyme-linked immunosorbent assay (ELISA).
    No preview · Article · Jan 2014
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    ABSTRACT: Introduction: Cancer of the endometrium is the most common gynaecologic malignancy in the developed countries. No tumour markers of appropriate sensitivity and specificity have been identified yet for diagnosing and monitoring the treatment of endometrial cancer. Heat shock proteins (Hsp) are known to be excessively expressed in a number of malignancies in humans, including gynaecological cancers. The presence of Hsp and anti-Hsp antibodies in the serum of female patients with gynaecological cancers is a very new area of research. Purpose: This study is intended to investigate concentrations of anti-heat shock protein immunoglobulin G (IgG) serum antibodies in patients suffering from endometrial cancer. Materials and methods: The study included 59 female patients with preliminary diagnosis of endometrial cancer based on histopathological examination. The control group consisted of 54 healthy female subjects attending routine gynaecological examination. Blood samples were collected for routine preoperative testing prior to the beginning of anticancer therapy. Antiheat shock protein immunoglobulin G (IgG) serum antibodies (anti-Hsp65) levels were measured by means of an immunoenzymatic test (ELISA). The distribution of variables was verified for compliance using the Shapiro-Wilk test. Inter-group comparisons were analysed with nonparametric Kolmogorov-Smirnov test and Mann-Whitney U-test. P<0.05 was considered statistically significant. Results: Average levels of anti-Hsp65 IgG serum antibodies in women with endometrial cancer were found to be significantly higher than in healthy controls. It was also demonstrated that the more clinically advanced tumour stage, the higher the serum levels of anti-heat shock protein serum antibodies. Conclusions: Anti-heat shock protein (HSP-65) antibodies levels are elevated in women with endometrial cancer. Testing the levels of Hsp65 and anti-Hsp65 antibodies may be of predictive value in diagnosing and monitoring the treatment of this type of cancer.
    No preview · Article · Jan 2014
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    ABSTRACT: Diabetes mellitus, as a risk factor for endometrial cancer (EC), causes an increase in insulin and IGF-1 concentrations in the blood serum. The increase in insulin and IGF-1 are considered mitogenic factors contributory to cancer development. Studies suggest that metformin has preventive activity, decreasing mortality and the risk of neoplasms. Since estrogen (ER), progesterone (PR) and IGF-1 (IGF-1R) receptor expression and beta-catenin and PAX-2 mutations are significant in the development of endometrial cancer, it was decided to study these factors in patients with endometrial cancer and type 2 diabetes mellitus (DM2), and to establish the effects of metformin on their expression. The expression of ER, PR, IGF-1R, beta-catenin and PAX-2 have been immunohistochemically investigated in 86 type I endometrial cancer specimens. Patients were grouped according to the presence of DM2 and the type of hypoglycemic treatment administered. Comparing EC patients with DM2 and normal glycemic status, we found increased IGF-1R expression in women with DM2. A decrease in ER expression was noted in women with EC and DM2 receiving metformin as compared to women treated with insulin (p = 0.004). There was no statistically significant difference in PR, IGF-1R, beta-catenin and PAX-2 expression among women receiving metformin and other hypoglycemic treatment. Although epidemiological studies suggest the beneficial role of metformin in many human cancers, there are still few studies confirming its favorable effect on endometrial cancer. Decreased ER expression in patients receiving metformin needs further research to allow evaluation of its clinical significance.
    Full-text · Article · Dec 2013 · Diabetology and Metabolic Syndrome
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    ABSTRACT: Abstract Objective: The aim of the study was a randomized comparison of the outcomes of intrauterine myelomeningocele repairs (IUMR) in type II Chiari malformation (II CM) fetuses with clinical data of newborns and infants operated on postnatally. Methods: The study group (SG) comprised of 46 pregnant women whose type II CM children underwent IUMR, while 47 pregnant women whose type II CM children were operated on postnatally constituted the control group (CG). A total of 24 SG and 20 CG patients reached the endpoint of the study. Results: High incidence of PROM (24 (52.2%), CI 3.74 (1.69-8.26) (p<0.001) was noted in the group of prenatal surgeries as compared to controls. The need for VP shunt implantation was statistically significantly lower (p<0.008) in the group of children after IUMR as compared to controls (5 (27.8%) and 16 (80%), respectively, CI 0.35 (0.16-0.75). None of the postnatally treated CG children can walk without adaptive equipment. In contrast, two children from the study group (2 (11.1%) CI 1.86 (1.00-3.48) p<0.048) are able to walk independently. Conclusions: Prenatal MMC closure significantly lowers further adverse evolution of the type II Chiari malformation. Further studies are needed, especially on preventive measures for preterm labor and IPPROM in the postoperative course of IUMR.
    Full-text · Article · Oct 2013 · The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
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    Preview · Article · May 2013 · Current Gynecologic Oncology
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    ABSTRACT: Marfan syndrome is an autosomal dominant disorder of connective tissue with up to 25% of cases related to a spontaneous mutation. It has been associated with perinatal loss, preterm labor and, potentially a rupture of the maternal aortic arch. We present a case of a woman diagnosed with Marfan syndrome after a miscarriage of her first pregnancy. At the time of diagnosis she had mild aortic bulb dilation and insufficiency of the mitral and tricuspid valves. She underwent cardiosurgical correction, after which she had two uneventful pregnancies. This case suggests that preconceptional correction of valve defects in women with Marfan syndrome may decrease the risk of cardiac decompensation during future pregnancies. Additionally close clinical follow up and the appropriate use of beta-adrenergic blockade may decrease the risk of aortic rupture, a significant risk factor for mortality in pregnant women.
    No preview · Article · Apr 2013 · Ginekologia polska
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    ABSTRACT: Background: Lichen sclerosus (LS) is an immune-mediated chronic dermatosis of perimenopausal women. Inflammatory infiltration has been suggested as a potential cause of LS changes in epidermis and dermis. Photodynamic therapy (PDT) is a specific noninvasive treatment based on detection and subsequent selective destruction of LS-affected tissues that are marked by a photosensitizer. Immunomodulatory and cytotoxic effects of PDT are responsible for inducing several immunologic reactions which leads to inflammatory condition improvement. The aim of the study was to determine the efficacy of photodynamic therapy for genital LS in correlation to protein expression involved in inflammatory infiltration - CD3, CD4, CD8, CD57, Granzyme B and Fascin. Material and methods: A group of patients has been treated for active LS disease, diagnosis was histologically confirmed in skin biopsies. The skin biopsies were taken and analyzed with antibodies: CD3, CD4, CD8, CD57, Granzyme B and Fascin before and after PDT treatment. Results: The study shows the efficacy of PDT in LS treatment including changes to CD3, CD4, CD57, Fascin, Granzyme B and the lymphocytic infiltration in skin sections. The proliferation index of CD4 did not differ before and after PDT. Conclusions: The PDT is a very effective therapeutic method in LS treatment.
    No preview · Article · Apr 2013 · Menopausal Review
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    ABSTRACT: Vaginal cancer is a rare neoplasm and in about 65% to 90% of cases it is a metastatic one. It is diagnosed in women aged from 60 to 70 years, but it can also appear in younger patients. Squamous cell carcinoma is the most frequently found histopathological type. Risk factors for the development of vaginal cancer are thought to be similar to those of cervical cancer. We present a case of a 52-year old woman with vaginal cancer treated with brachytherapy. Due to neoplasm recurrence, additional examinations were performed in the course of one year. The patient was qualified for total pelvic exenteration. A leakage from the abdominal wound was observed post operatively. It was successfully noninvasively treated. In 3 months time she was hospitalized because of the superficial abdominal abscess which was located next to the left ureterostomy. The patient undergoes regular gynecological, surgical and urological control examinations. So far the results do not show any recurrence of the neoplastic process. Total pelvic exenteration should be considered in selected groups of patients.
    No preview · Article · Feb 2013 · Ginekologia polska
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    ABSTRACT: We present case report of 51-year-old female patient admitted to surgery ward because of presence of pyogenic discharge in perianal region since 7 months. Eighteen months earlierthe patient underwent stress urinary incontinence procedure with use of TVT synthetic implant. Diagnosis of wast, bilateral, composite vagino-perianal fistule was made. After carrying out four operations with two stage sling removal final postfistule wound healing was obtained.
    No preview · Article · Jan 2013 · Wiadomości lekarskie (Warsaw, Poland: 1960)
  • I. Gabriel · S. Horak · A. Olejek

    No preview · Article · Nov 2012 · Journal of Minimally Invasive Gynecology
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    ABSTRACT: Aim of study: Lichen sclerosus (LS) is a chronic inflammatory disease of skin and mucous membranes. Autoimmune background has been suggested as a potential pathomechanism of LS development. The aim of our study was to evaluate concentration of thyroid antibodies (anti-TSHR, anti-TG and anti-TPO), antinuclear antibody titers and presence of Borrelia burgdorferi antibodies in patients with vulvar lichen sclerosus treated with photodynamic therapy. Materials and methods: Serum samples of 64 patients with diagnosed vulvar LS were taken in order to evaluate presence of Borrelia burgdorferi antibodies, antinuclear antibody titers and concentration of anti-TSHR, anti-TG and anti-TPO antibodies before and after PDT therapy (10 courses). As a topical photosensitizer 5% 5-ALA was applied. For ANA evaluation we used an indirect immunofluorescence test (Euroimmun Mosaic HEp2/monkey liver). In 25 cases we performed the ANA Profile 3 Euroline test to identify particular kinds of ANA. were shown in the identified fluorescent patterns of ANA before and after PDT therapy. IgG and IgM Anti-Borrelia Results: In the case of anti-TSHR, anti-TPO and anti-TG antibodies, results of quantity values were not statistically significant. We noted a statistically significant decrease of ANA titers after PDT therapy. No diferences antibodies were found only in 1 and in no subjects, respectively. Conclusions: PDT therapy influences ANA but not anti-TSHR, anti-TPO, anti-TG concentration among patients with diagnosed vulvar LS. In our opinion, Borrelia burgdorferi does not seem to play a significant role in vulvar LS pathogenesis.
    No preview · Article · Aug 2012 · Menopausal Review
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    ABSTRACT: The present study has focused on the identification of the differences between expression patterns of kinin-dependent genes in endometrial cancer with the use of real-time quantitative reverse transcription polymerase chain reaction and oligonucleotide microarray. The study group consisted of 50 endometrium samples collected from women with endometrial cancer. Gene expression of kinin receptors BR1 and BR2 was evaluated with real-time quantitative reverse transcription polymerase chain reaction. The analysis of the expression profile of genes related to the kinin mitogenic signal transduction pathway was performed using HG-U133A oligonucleotide microarrays. The transcriptional activity of the B1 receptor for kinins increased in patients with grade 1 (G1) and grade 2 (G2) endometrial cancer when compared to the control group, whereas it decreased in patients with grade 3 (G3) endometrial cancer. The expression of the B2 receptor showed a growing trend reaching the peak in the G2, whereas G3 was characterized by a decrease in the gene transcriptional activity. Significant differential gene expression was recorded for GNB1, PRKAR1A, KRAS, MAP2K2, GNG5, MAPK1, ADCY9, GNG11, JUN, PRKCA, PRKACB, FOS, PLCB4, ADCY8, and GNG12. The expression changes in kinin-dependent genes might cause disturbance in the underlying biological processes, which could be important for the pathogenesis of endometrial cancer. This will eventually help to improve treatment strategies for patients with endometrial cancer in the future.
    No preview · Article · Jun 2012 · International Journal of Gynecological Cancer
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    ABSTRACT: Endometrial cancer is one of the most frequently diagnosed malignant neoplasms among women. In Poland, it is in the fourth place in terms of incidence. The highest morbidity concerns women aged 50-70 years, however it may also appear in women in their reproductive period. Endometrial cancer concerns about 3% of premenopausal women. We present a case of a 25-year-old patient who underwent endometrial curettage because of irregular menstrual bleeding for the last 5 months. Histopathology revealed endometrial cancer. We attempted to apply a conservative treatment. During the next 6 months the patient was treated with lynestrenol. After one month of hormonal therapy endometrial curettage was repeated. In histopathology endometrial tissues corresponding to the hormonal treatment were found. After 6 months of treatment hysteroscopy with endometrial biopsy followed by endometrial curettage, were performed. Hormonal treatment resulted in disease regression. About 5 months after successful treatment the patient conceived spontaneously. One year after she gave birth to her first child, she conceived spontaneously once more. Both children were born vaginally. In selected cases of atypical hyperplasia and early endometrial cancer in young women the attempt of hormonal treatment is acceptable.
    No preview · Article · Mar 2012 · Ginekologia polska

Publication Stats

267 Citations
63.68 Total Impact Points

Institutions

  • 2001-2014
    • Medical University of Silesia in Katowice
      • Department of Obstetrics and Gynecology
      Catowice, Silesian Voivodeship, Poland
  • 2005-2013
    • Silesian University of Technology
      Gleiwitz, Silesian Voivodeship, Poland
  • 2012
    • Pomorski Uniwersytet Medyczny
      Stettin, West Pomeranian Voivodeship, Poland