Publications (14)21.99 Total impact
- [Show abstract] [Hide abstract] ABSTRACT: Enterococcus faecalis is a pathogenic microorganism. The aim of this investigation was to study the antibiotic susceptibility of the strains isolated in Cordoba in a 20-month period (January 2004 to August 2005). Susceptibility rates to betalactamics were 98% to ampicillin and 99% to amoxicillin/clavulanic acid; high-dose aminoglycosides (streptomycin 1000 microg and gentamycin 500 microg) obtained 56% and 76%, respectively. We found no strains resistant to glycopeptides (vancomycin and teicoplanin) or to linezolid.
- [Show abstract] [Hide abstract] ABSTRACT: We studied the antimicrobial susceptibility of bacteria isolated from sputum from patients with cystic fibrosis in our hospital during 2001 and 2002. The most frequently isolated microorganisms were Staphylococcus aureus (59.89%), Pseudomonas aeruginosa (49.45%), Stenotrophomonas maltophilia (4.9%) and Haemophilus influenzae (3.8%). The rate of methicillin-resistant S. aureus was 18%, and no strains with low susceptibility to glycopeptides were found. Carbapenems showed the highest activity against P. aeruginosa, although this did not reach 100%.
- [Show abstract] [Hide abstract] ABSTRACT: Study of antifungal sensitivity can help in treatment screening and evaluation of patients suffering from some fungal infections. The purpose of this study is to compare fluconazole MICs obtained by E-test and agar dilution with the NCCLS method. The in vitro activity of fluconazole against 158 yeast strains by three systems: E-test, agar dilution and the microbroth NCCLS M27P method. A correlation between 84 to 100% was observed the degree varying in the result among different species. The E-test was found to be comparable to the NCCLS M27P microbroth method, was easier to perform and provides MIC result for Candida species within 24 h.
- [Show abstract] [Hide abstract] ABSTRACT: The E test is a quantitative technique for determining antimicrobial susceptibility; it is based on the diffusion of a stable predetermined gradient of a specific antifungal agent along an inert plastic strip which is applied to a solid culture medium. The E test is an alternative method for determining inhibitory concentrations of antimicrobial agents. The aim of the present study was to determine the in vitro susceptibility of different yeasts to 5 antifungal agents using the E test. The antifungal susceptibility of 87 strains, 73 of which were yeasts obtained from clinical samples, 11 were Prototheca strains and 3 controls, were examined using a gradient diffusion technique (E test). Of these isolates, 13% of Candida albicans were resistant to flucytosine, and 20%, 17% and 33% showed resistance to ketoconazole, itraconazole and fluconazole, respectively. All Candida krusei isolates showed resistance to flucytosine and fluconazole. The Candida parapsilosis and Candida tropicalis strains all presented in vitro susceptibility to all the agents tested. Prototheca strains were only susceptible in vitro to amphotericin B. It was found that the E test was easy to perform, and provided MIC values similar to those obtained with other well documented techniques. It was therefore concluded that the E test could be implemented as a standard procedure in clinical mycology laboratories.
- [Show abstract] [Hide abstract] ABSTRACT: The in vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae (M. chelonei) to ticarcillin in combination with calvulanic acid (CA) was studied by the agar dilution method. All the M. tuberculosis, M. bovis, and M. africanum strains were inhibited at a ticarcillin concentration of 32 micrograms/ml or lower in combination with 5 micrograms of CA. M. chelonae and M. avium strains proved resistant to more than 128 micrograms of ticarcillin plus 5 micrograms of CA per ml. M. fortuitum strains needed 128 micrograms of ticarcillin plus 5 micrograms of CA to inhibit approximately 30% of the isolates.
- [Show abstract] [Hide abstract] ABSTRACT: The in vitro susceptibility of Mycobacterium fortuitum and Mycobacterium chelonei to cefmetazole was studied by the agar dilution method. At a concentration of 16 micrograms/ml or lower, 44 isolates (96%) of M. fortuitum and 8 isolates (40%) of M. chelonei were inhibited.
- [Show abstract] [Hide abstract] ABSTRACT: On a étudié l'inhibition in vitro de 37 souches de Mycobacterium fortuitumpar divers antibiotiques et sulfamides, à l'aide de la méthode de diffusion en milieu gélosé de Mueller-Hinton, en employant des disques d'antibiotiques dont les charges sont celles habituellement utilisées pour les autres bactéries. Toutes les souches se sont montrées résistantes à la furadoïne, à la mandélamine, au métronidazole, à l'acide nalidixique et à l'acide oxolinique. La nitro-5-hydroxy-8-quinoléine, l'acide pipémidique et les sulfamides ont donné le plus grand nombre d'inhibitions.
- [Show abstract] [Hide abstract] ABSTRACT: The in vitro action of sisomicin, gentamicin and tobramycin against 62 strains of Mycobacterium fortuitum and 20 strains of M. chelonei was investigated. At a drug concentration of 4 micrograms/ml, 31, 31 and 11%, respectively, of M. fortuitum strains and 90, 25 and 15%, respectively, of M. chelonei strains, were inhibited.
- [Show abstract] [Hide abstract] ABSTRACT: A simple, new test to differentiate species in the Mycobacterium fortuitum complex by determining susceptibility to pipemidic acid is described. A 20-mug pipemidic acid disk provides a rapid and reliable means of distinguishing M. fortuitum from M. chelonei.
- [Show abstract] [Hide abstract] ABSTRACT: Enterococcus faecalis es un microorganismo patógeno para el hombre. El objetivo de este estudio fue conocer la sensibilidad a diferentes antibióticos de los aislamientos obtenidos en la provincia de Córdoba en un periodo de 20 meses, de enero de 2004 a agosto de 2005. Los porcentajes de sensibilidad para los betalatámicos fueron del 98% a ampicilina y del 99% a amoxicilina-ácido clavulánico. Los aminoglucósidos estreptomicina y gentamicina, probados a altas dosis (estreptomicina 1000 µg y gentamicina 500 µg), obtuvieron un porcentaje de sensibilidad del 56% y el 76%, respectivamente. No se encontró ninguna cepa resistente a glucopéptidos (vancomicina y teicoplanina) ni a linezolid.
- [Show abstract] [Hide abstract] ABSTRACT: A study was made of the in vitro susceptibility of 37 strains of Mycobacterium fortuitum to 14 antimicrobial agents using disc diffusion techniques on Mueller-Hinton agar plates. Nitro-5-hydroxy-8-quinoleine and pipemidic acid inhibited all strains, while furantoin, mandelamine, metronidazole, nalidixic acid, oxolinic acid and trimethoprim proved to be inactive. In addition, various sulphonamides inhibited 59% of the strains tested. Sulphadiazine inhibited 54.1%, sulphafurazole 64.9%, sulphamethizole 64.9%, sulphametoxypyridazine 62.2% and sulphisoxazole 59.5%. Trimethoprim-sulphamethoxazole was effective against 48.6% of the strains due to its sulph component. The sensitivity to sulphonamides varied in accordance with the origin of the strains, those of human origin being generally much more susceptible than those of non-human origin.
Hospital Universitario Reina SofíaCordoue, Andalusia, Spain
University of Cordoba (Spain)
Cordoue, Andalusia, Spain
- Department of Microbiology