Yuanliang Wang

Chongqing University, Ch’ung-ch’ing-shih, Chongqing Shi, China

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Publications (63)128.13 Total impact

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    ABSTRACT: Damaged cartilage has poor self-healing ability and usually progresses to scar or fibrocartilaginous tissue, and finally degenerates to osteoarthritis (OA). Here we demonstrated that one of alternative isoforms of IGF-1, mechano growth factor (MGF) acted synergistically with transforming growth factor β3 (TGF-β3) embedded in silk fibroin scaffolds to induce chemotactic homing and chondrogenic differentiation of mesenchymal stem cells (MSCs). Combination of MGF and TGF-β3 significantly increased cell recruitment up to 1.8 times and 2 times higher than TGF-β3 did in vitro and in vivo. Moreover, MGF increased Collagen II and aggrecan secretion of TGF-β3 induced hMSCs chondrogenesis, but decreased Collagen I in vitro. Silk fibroin (SF) scaffolds have been widely used for tissue engineering, and we showed that methanol treated pured SF scaffolds were porous, similar to compressive module of native cartilage, slow degradation rate and excellent drug released curves. At 7days after subcutaneous implantation, TGF-β3 and MGF functionalized silk fibroin scaffolds (STM) recruited more CD29+/CD44 + cells (P < 0.05). Similarly, more cartilage-like extracellular matrix and less fibrillar collagen were detected in STM scaffolds than that in TGF-β3 modified scaffolds (ST) at 2 months after subcutaneous implantation. When implanted into articular joints in a rabbit osteochondral defect model, STM scaffolds showed the best integration into host tissues, similar architecture and collagen organization to native hyaline cartilage, as evidenced by immunostaining of aggrecan, collagen II and collagen I, as well as Safranin O and Masson's trichrome staining, and histological evalution based on the modified O'Driscoll histological scoring system (P < 0.05), indicating that MGF and TGF-β3 might be a better candidate for cartilage regeneration. This study demonstrated that TGF-β3 and MGF functionalized silk fibroin scaffolds enhanced endogenous stem cell recruitment and facilitated in situ articular cartilage regeneration, thus providing a novel strategy for cartilage repair. Copyright © 2015 Elsevier Ltd. All rights reserved.
    No preview · Article · Jun 2015 · Biomaterials
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    ABSTRACT: Local application of bone morphogenetic protein 2 (BMP2) is known to promote large bone defect healing and BMP2-initiated bone regeneration could be enhanced by an additional mechanical stimulation. The C-terminal 24-a.a. peptide of mechano growth factor (MGF24E), a mechanical-sensitive molecule, has been demonstrated to promote bone healing. Here, we propose a hypothesis that MGF24E could also improve the osteogenic efficacy of BMP2 by regulating the signaling events in the BMP pathway. To confirm the hypothesis, the potentials of MGF24E, BMP2 and BMP2/MGF24E combination treatments on the phosphorylation of Smad 1/5/8, the downstream osteogenesis-related gene expression and osteoblasts mineralization, are investigated with or without the blocking of Smad 5 siRNA. Furthermore, 15-mm rabbit radial bone defects were healed with the cytokine treatments and then evaluated by radiographic examination, histological assessment and immunohistochemical analysis. MGF24E could enhance the BMP2-induced Smad signaling pathway by upregulating the p-Smad protein expression and the downstream osteogenic gene expression. An amount of 5 nM BMP2 in a sub-25 nM concentration of MGF24E medium achieved a higher expression for ALP mRNA and a greater calcium mineral content compared with BMP2 alone. Nevertheless, the inhibition of the MGF24E-regulated BMP pathway could block osteogenesis induced by the dual treatment. In vivo, MGF24E treatment upregulated the endogenous BMP2 expression and the addition of MGF24E into the BMP2 treatment remarkably enhanced the bone mineral density (BMD), the radiographic scores and the histological restoration of the regenerated tissue against BMP2 treatment, suggesting a new strategy for BMP2 in bone defect healing.
    No preview · Article · Apr 2015 · Cell and Tissue Research
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    Yan Li · Liqian Wang · Jinchuan Wu · Yufei Ma · Jinfeng Wang · Yuanliang Wang · Yanfeng Luo

    Preview · Article · Jan 2015 · Materials Letters
  • Xudong Tu · Yuanliang Wang · Maolan Zhang · Jinchuan Wu

    No preview · Article · Jan 2015 · IEEE/ACM Transactions on Computational Biology and Bioinformatics
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    ABSTRACT: Low fluid shear stress (FSS) is the mechanical environment encountered by osteoblasts in implanted bones or native bones of bed-rest patients. High sensitivity of osteoblasts to low FSS is beneficial to osteogenesis. We hypothesize that this sensitivity might be regulated by chemical microenvironment provided by scaffolds. To confirm this hypothesis, self-assembled monolayers (SAMs) were used to provide various surface chemistries including OH, CH3 , and NH2 while parallel-plate fluid flow system produced low FSS (5 dynes/cm(2) ). Alterations in S-phase cell fraction, alkaline phosphatase (ALP) activity, fibronectin (Fn) and collagen type I (COL I) secretion compared to those without FSS exposure were detected to characterize the sensitivity. Osteoblasts on OH and CH3 SAMs demonstrated obvious sensitivity while on NH2 SAMs negligible sensitivity was observed. Examination of the cell aspect ratio, orientation and focal adhesions before and after FSS exposure indicates that the full spreading and robust focal adhesions on NH2 SAMs should be responsible for the negligible sensitivity through increasing the cell tolerance to low FSS. Despite the higher sensitivity, the Fn and COL I depositions on both OH and CH3 SAMs after FSS exposure were still less than on NH2 SAMs without FSS exposure. These results suggest that elaborate design of surface chemical compositions is essential for orchestration of surface chemistry with low FSS to realize both high sensitivity and high matrix secretion, facilitating formation of functional bone tissues in implanted bone.
    No preview · Article · Nov 2014 · Journal of Biomedical Materials Research Part A
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    Yan Li · Liqian Wang · Jinchuan Wu · Yufei Ma · Jinfeng Wang · Yuanliang Wang · Yanfeng Luo
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    ABSTRACT: Poly(vinyl alcohol) and hyaluronic acid derived hydrogel was synthesized by a novel synthesis route using click reaction under mild condition. First, we synthesized two kinds of soluble polymers by linking propargylamine and l-cysteine on the poly(vinyl alcohol) and hyaluronic acid, respectively. The polymers were named as PVAPA and HASH, and characterized by 1H NMR and Ellman’s test. The hydrogels were obtained through thiol-yne click reaction in the presence of photoinitiator after 3 min UV light. The swelling ratio in various pH solution and rheology of hydrogels were detected to confirm that the hydrogels process a certain pH-sensitive property and a stable network system. The click reaction of PVAPA and HASH provided a simple and versatile method to get hydrogel network under a mild condition and have a vast potential for biomedical applications.
    Preview · Article · Nov 2014 · Materials Letters
  • Kun Peng · Jiaoxia Sun · Yuanliang Wang · Yuxiao Li · Bingbing Zhang · Bing Wang
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    ABSTRACT: In this study, novel disulfide cross-linked hydrogels were designed and synthesized. First, ethylenediaminetetraacetic dianhydride reacted with butanediamine and amino-terminated polyethylene glycol via N-acylation reaction to give biocompatible poly(amic acid) (PAA) with pendant carboxyl groups; then, the amino groups of cystamine reacted with carboxyl groups of PAA to generate disulfide cross-linked network polymer (PAA-SS). Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance imaging, gel permeation chromatography with multiangle laser light scattering, potentiometric titration, rheology, hydrolytic degradation, morphology, porosity, and in vitro biocompatibility studies were used to qualitatively and quantitatively characterize the obtained polymers. The results indicated that the equilibrium swelling ratio of the PAA-SS decreased with the increase in Rm. The PAA-SS provided good mechanical strength to maintain their integrity, and the storage modules (G′) of the hydrogels can be adjusted by Rm. The PAA-SS presented co-continuum pores, and the pore size correlated with the cross-linking degree. The degradation of PAA-SS could be controlled by regulating the concentration of dithiothreitol. Particularly, the PAA-SS possessed an excellent biocompatibility, as the average proliferating rate of osteoblasts on PAA-SS was appreciably higher than that on PAA and glass coverslips. In conclusion, the above obtained results demonstrate that the performance of the PAA-SS outbalance and facilitate the application in biomedical region, particularly in bone tissue regeneration. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40930.
    No preview · Article · Oct 2014 · Journal of Applied Polymer Science
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    Xufeng Niu · Pin Chen · Xiaoling Jia · Lizhen Wang · Ping Li · Li Yang · Yuanliang Wang · Yubo Fan
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    ABSTRACT: Mechano growth factor (MGF) and its C-terminal E-peptide with 24 amino acids, MGF-Ct24E, have superiority in resolving the delayed or failed bone repair derived from shortness of suitable biomechanical stimulation. The chitosan/tripolyphosphate microspheres encapsulated with MGF-Ct24E (CS/TPP/MGF-Ct24E) are prepared using emulsion-ionic cross-linking method in order to achieve the sustained release and preserve the bioactivity of MGF-Ct24E. The microspheres are micron-sized and spherical in shape with smooth surface morphology. The TPP component disintegrates in advance of CS matrix and the MGF-Ct24E maintains sustained delivery during in vitro hydrolytic degradation. With the disappearance of TPP, the total weight loss of CS/TPP/MGF-Ct24E is 32% and the release amount of MGF-Ct24E reaches 84.6% after degrading for 2 weeks. In vitro bioactivity assays reveal that the MGF-Ct24E can accelerate MC3T3-E1 cells proliferation and delay their differentiation as well. The encapsulated MGF-Ct24E shows long-term effects after being loaded in the CS/TPP microspheres and the cells exhibit excellent morphology on the surface of microspheres. The continuous delivery of MGF-Ct24E provides a new perspective on resolving the unsatisfactory bone reconstruction associated with microgravity and stress shielding.
    Full-text · Article · Apr 2014 · International Journal of Pharmaceutics
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    ABSTRACT: Piperazine-based polyurethane ureas (P-PUUs) with piperazine (PP) units as the functional ingredients have been designed to explore a novel polymeric biomaterial with the controllable degradation for bone repair in this study. P-PUUs were prepared upon the chain-growth polymerization of poly (D, L -lactic acid) diol (PDLLA diol), 1, 6-hexamethylene diisocyanate (HDI) where PP functioned as the chain extender. The investigation of in vitro degradation of P-PUUs was performed and the mechanical properties of P-PUUs during degradation were calculated. The results revealed that manipulating the ratio of PDLLA diol: HDI: PP could obtain the precise number of PP units (Num(pp)) in P-PUUs which may significantly impact the degradation stability and the degradation rate of P-PUUs. The negative linear correlation between the degradation rate and the Num(pp) of P-PUUs has been proved, suggesting the P-PUUs were endowed with controllable degradation. Such a linear correlation is expected to be applied to bone repair where the specific degradation rate of P-PUUs for the specific bone defect can be precisely predicted once the Num(pp) and the bone repairing period are known. Additionally, P-PUUs with higher Num(pp) exhibited remarkable mechanical properties during degradation which allowed polymers to provide more persistent mechanical support to bone repair.
    Full-text · Article · Feb 2014 · Polymer
  • Yan Li · Yanfeng Luo · Ke Huang · Juan Xing · Zhao Xie · Manping Lin · Li Yang · Yuanliang Wang
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    ABSTRACT: Natural bone tissue receives chemical and mechanical stimuli in physiological environment. The effects of material chemistry alone and mechanical stimuli alone on osteoblasts have been widely investigated. This study reports the synergistic influences of material chemistry and flow shear stress (FSS) on biological functions of osteoblasts. Self-assembled monolayers (SAMs) on glass slides with functional groups of OH, CH3, and NH2 were employed to provide various material chemistries, while FSS (12 dynes/cm(2)) was produced by a parallel-plate fluid flow system. Material chemistry alone had no obvious effects on the expressions of ATP, nitric oxide (NO), and prostaglandin E2 (PGE2), whereas FSS stimuli alone increased the production of those items. When both material chemistry and FSS were loaded, cell proliferation and the expressions of ATP, NO and PGE2 were highly dependent on the material chemistry. Examination of the focal adhesion (FA) formation and F-actin organization of osteoblasts before FSS exposure indicates that the FA formation and F-actin organization followed similar chemistry-dependence. The inhibition of FAs and/or disruption of F-actins eliminated the material dependence of FSS-induced ATP, PGE2 and NO release. A possible mechanism is proposed: material chemistry controls the F-actin organization and FA formation of osteoblasts, which further modulates FSS-induced cellular responses.
    No preview · Article · Sep 2013 · Archives of Biochemistry and Biophysics
  • Yan Li · Yanfeng Luo · Zhao Xie · Juan Xing · Manping Lin · Li Yang · Yuanliang Wang · Ke Huang
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    ABSTRACT: Osteoblasts on implanted biomaterials sense both substrate chemistry and mechanical stimulus. The effects of substrate chemistry alone and mechanical stimulus alone on osteoblasts have been widely studied. This study investigates the optimal combination of substrate chemistry and 12dyn/cm(2) physiological flow shear stress (FSS) by examining their influences on primary rat osteoblasts (ROBs), including the releases of ATP, nitric oxide (NO), and prostaglandin E2 (PGE2). Self-assembled monolayers (SAMs) on glass slides with -OH, -CH3, and -NH2 were employed to provide various substrate chemistries, whereas a parallel-plate fluid flow system produced the physiological FSS. Substrate chemistry alone exerted no observable effects on the releases of ATP, NO, and PGE2. Nevertheless, when ROBs were exposed to both substrate chemistry and FSS, the ATP releases of NH2 were upregulated about 12-fold compared to substrate chemistry alone, while the ATP releases of CH3 and OH was similarly increased 7-fold at the peak. Similar trends were observed for the releases of NO and PGE2. The expressions of ATP, NO, and PGE2 followed the pattern of NH2-FSS>Glass-FSS>CH3-FSS≈OH-FSS. ROBs on NH2 produced the optimal combination of substrate chemistry with the physiological FSS. The F-actin organization and focal adhesion (FA) formation of ROBs on various SAMs without FSS were examined. NH2 produced the best results whereas CH3 and OH produced the worst ones. Inhibition of FAs and/or disruption of F-actin significantly decreased the releases of FSS-induced PGE2, NO, and/or ATP. Consequently, a mechanism was proposed that the best F-actin organization and FA formation of ROBs on NH2 lead to the optimal combination of substrate chemistry with the 12dyn/cm(2) physiological FSS. This mechanism gives guidance for the design of implanted biomaterials and bioreactors for bone tissue engineering.
    No preview · Article · Jul 2013 · Colloids and surfaces B: Biointerfaces
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    ABSTRACT: As a growth repair factor, mechano-growth factor (MGF) and its 24 amino acid peptide analogs corresponding to the unique C-terminal E-domain (MGF-Ct24E) positively regulate bone regeneration. MGF-Ct24E was introduced into the poly(D, L-lactic acid) (PDLLA) to improve bone regeneration in our previous study. MGF-Ct24E-grafted PDLLA was chemically characterized and showed potential as a biofunctional polymer. In this study, we evaluated the cytocompatibility of MGF-Ct24E chemically grafted and physically blended with maleic anhydride modified PDLLA, relative to maleic anhydride modified PDLLA (MPLA) as the control. The surface properties of these three polymer films were characterized with scanning electron microscopy and X-ray photoelectron spectroscopy. Rat calvarial osteoblasts were seeded on the three polymer films, and cell adhesion, spreading, and proliferation were assessed with an inverted microscope, laser scanning confocal microscope, and a cell counting kit-8, respectively. The alkaline phosphatase activity and extracellular calcium production were exploited to characterize the differentiation and mineralization of rat calvarial osteoblasts on various polymer films. The results revealed that compared with MPLA, MGF-Ct24E-MPLA, and MGF-Ct24E/MPLA blends promoted adhesion, spreading, proliferation, and the later differentiation and mineralization process of rat calvarial osteoblasts. In addition, the positive effect of MGF-Ct24E-MPLA on rat calvarial osteoblasts was maintained longer than the MGF-Ct24E/MPLA blends. In conclusion, MGF-Ct24E-MPLA synthesized chemically might be a promising biomaterial for bone tissue engineering.
    No preview · Article · May 2013 · Journal of Biomaterials Science Polymer Edition
  • Maolan Zhang · Yuanliang Wang · Jiaoxia Sun · Jinchuan Wu · Weiwei Yan · Yuxi Zheng
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    ABSTRACT: A novel antibacterial piperazine derivative (PE-B) has been prepared via the nucleophilic substitution reaction of poly(ethylenediaminetetraacetic dianhydride-co-piperazine) (PE) with n-butyl bromide. PE was synthesized by a condensation polymerization reaction of ethylenediaminetetraacetic dianhydride (EDTAD) and piperazine (PA). The thermoresponsive test results showed that the PE was thermoresponsive; however, when it was quaternized by n-butyl bromide, this performance disappeared. The antibacterial test results showed that both the PE and PE-B exhibited potent inhibitory activity on S. aureus and E. coli. Specifically, PE had better antibacterial ability against S. aureus, but PE-B showed similar antibacterial activity against S. aureus and E. coli.
    No preview · Article · Mar 2013 · Chemistry Letters
  • Ya Fu · Meina Huang · Yanfeng Luo · Chengbo Hu · Yuanliang Wang
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    ABSTRACT: RGDS-PDLLA is a biomimetic material obtained by covalently incorporating RGDS adhesion peptide. RGDS-PDLLA was prepared on basis of BDPLA (butanediamine-grafted polyactic acid). In present work, we prepared RGDS-PDLLA with gradient concentration RGDS and evaluated the cytocompatibility of gradient RGDS-PDLLA and took a novel method for studying the effective concentration of RGDS after grafting on BDPLA. When the concentration of RGDS in the RGDS-grafted-PDLLA (RGDS-PDLLA) varied from 0.142 to 0.302 μmol/g, cell cytocompatibility of osteoblasts was altered depending on the concentration of RGDS. 0.1 μmol/g RGDS on RGDSPDLLA (RGDS-PDLLA) membrane can effectively promoted cell attachment, cell spreading. The concentration of RGDS increased from 0.142 to 0.302 μmol/g, the adhesion of osteoblasts on RGDS-PDLLA membrane increased slightly. However, we can find that when the RGDS increased to 2.42 μmol/g, the cell on films increased. These results suggest that the cell adhesion is sensitive to the RGDS concentration. By the study, we can confirm the optimal concentration of RGDS on RGDS-PDLLA.
    No preview · Article · Feb 2013 · Asian Journal of Chemistry
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    Meina Huang · Yuanliang Wang · Zexuan Yan · Yanfeng Luo · Fusheng Pan
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    ABSTRACT: Shape memory poly(urethane urea) (SMPUU) can recover its preinstalled shape on body temperature and fill the gap between body and graft. This may be one key factor for preventing non-union and generate evident “shape memory deformation”. In the present paper, deformed-SMPUU was prepared, and atom force microscope, methylthiazolyl tetrazolium assay, laser scanning confocal microscope were used to characterize and to evaluate the effects of “shape memory deformation” on cytocompatibility. It was shown that “shape memory deformation” made micro scale “grooves” on SMPUU, promoting osteoblasts adhesion and proliferation, and even induced oriented spreading. This is beneficial to bone repair processs.
    Full-text · Article · Dec 2012 · Procedia Engineering
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    ABSTRACT: Mechano-growth factor (MGF) is an alternative splicing variant of Insulin-like growth factor I. MGF and its 24 amino acid peptide analog corresponding to the unique C-terminal E-domain (MGF-Ct24E) are the positive regulator for tissue regenesis in bone. A novel biomimetic poly(D, L-lactic acid) (PDLLA) modification was designed and synthesized based on MGF-Ct24E grafted maleic anhydride modified PDLLA (MPLA). MGF-Ct24Es were grafted into the side chain of MPLA via a stable covalent amide bond using 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as the condensing agent to produce biomimetic MPLA materials (MGF-Ct24E-MPLA). Fourier transform infrared spectrometry, amino acid analyzer, and elementary analysis were used to characterize the MGF-Ct24E-MPLA. The hydrophilicity of MGF-Ct24E-MPLA was evaluated by means of the water-uptake ratios and static water contact angle. Data revealed that the grafting efficiency of MGF-Ct24E was about 29.9%. MGF-Ct24E-MPLA had better hydrophilicity than PDLLA and MPLA. The osteoblasts behavior of proliferation, differentiation, and mineralization on PDLLA, MPLA, and MGF-Ct24E-MPLA films was investigated and the results indicated that the introduction of MGF-Ct24E could improve osteoblasts proliferation, mineralization, and delay differentiation. The MGF-Ct24E modified MPLA with higher bioactivity may have potential application for bone tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3496-3502, 2012.
    No preview · Article · Dec 2012 · Journal of Biomedical Materials Research Part A
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    Chengbo Hu · Yuanliang Wang · Ya Fu · Jiaoxia Sun · Moyuan Deng · Xiongzhao Xiang
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    ABSTRACT: The novel {2,4-bis(1,1-dimethylethyl)-6-{[(2-hydroxyethyl)imino]methyl}phenoxy}2 titanium(IV) complex {[(dME)2(HIM)P]2Ti(IV)} had been synthesized and characterized by EA, XRF, IR, and NMR methods. The [(dME)2(HIM)P]2Ti(IV) complex was reactive and promoted ring-opening polymerization (ROP) of d,l-lactide in the presence of benzyl alcohol (BnOH). And the results showed that ROP of d,l-lactide were produced upon addition of an excess (1–8 equiv.) of external BnOH. The complex showed a living and controlled fashion (M w/M n = 1.15–1.32) for ROP of d,l-lactide and could produce the poly(d,l-lactide) with average molecular weight (M n) up to 8.87 × 104 g mol−1. The mechanism study by 1H NMR spectrum of poly(d,l-lactide) with terminal benzyl ester group and [(dME)2(HIM)P]2Ti(IV) complex revealed that the polymerization proceeded through the traditional activated monomer mechanism and the acyl-oxygen bond cleavage mode of monomer. And the 13C NMR spectra and TG/DSC analysis showed that poly(d,l-lactide) was essential atactic and thermal stable polymer.
    Preview · Article · Sep 2012 · Polymer Bulletin
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    ABSTRACT: Folate-conjugated Dol-poly(d,l-lactic acid)-b-poly (ethylene glycol)-folate (Dol-PLA-PEG-FA), was synthesized from dodecanol-poly(d,l-lactic acid), amino-terminated poly(ethylene glycol) and folate. 1H NMR proved the successful synthesis of Dol-PLA-PEG-FA. Nanoparticles (NPs) were further fabricated from Dol-PLA-PEG-FA by using solvent evaporation-induced interfacial self-assembly method. The size, critical micelle concentration (CMC), cytotoxicity and selecting capability to cancer cells in vitro were examined for Dol-PLA-PEG-FA NPs. The size of NPs showed polymer concentration-dependent phenomenon in the fabrication process, and its polydispersity index (PDI) was very narrow. The CMC was determined as 1.995 × 10−4 g/L in aqueous solution, which is much lower than the reported CMC of block copolymer self-assemble micelles. The cytotoxicity evaluation revealed that the obtained NPs2 are non-toxic to either breast cancer cell or normal endothelial cells, and the cell uptake of NPs indicated that the NPs demonstrated much higher selecting capability to breast cancer cells compared to normal fibroblast cells. The possible receptor-mediated endocytosis pathway mechanism was proposed. Based on the above results, it could be concluded that Dol-PLA-PEG-FA polymer and its nanoparticles can be potentially used as a safe drug carrier with strong tumor cells targeting capability for tumor chemotherapy.
    No preview · Article · Jul 2012 · Colloids and surfaces B: Biointerfaces
  • Qin Peng · Juhui Qiu · Jiaoxia Sun · Li Yang · Bingbing Zhang · Yuanliang Wang
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    ABSTRACT: Mechano-growth factor (MGF) has emerged as an important mechanosensitive player in bone repair, but understanding of MGF function is hampered by the fact that MGF receptor and the underlying pathways remain unknown. In this study, fluorescein isothiocyanate (FITC)-labeled MGF-Ct24E (FITC-MGF) was used to determine the subcellular localization of MGF receptor in osteoblasts. After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis. Our results showed that the fluorescence intensity and cell population internalizing FITC-MGF increased with the concentration of FITC-MGF. And all the cells were labeled with fluorescence at 1 mg/ml. Notably, FITC-MGF had nuclear localization when osteoblasts were exposed to stretch and/or 50 ng/ml MGF-Ct24E added, compared to the evident cytoplasmic localization in the static culture group. The nuclear localization of FITC-MGF in response to mechanical loading was found to associate with high expression of proliferating cell nuclear antigen, suggesting MGF and its receptor could serve as potential messengers that replay information in nuclei to control cell proliferation.
    No preview · Article · Jul 2012 · Molecular and Cellular Biochemistry
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    ABSTRACT: Angiogenesis is crucial in wound healing. The administration of the C-terminal 24-a.a. peptide of mechano growth factor (MGF24E) has been previously demonstrated to induce more blood vessels in regenerating bone around defective areas compared with the control. Accordingly, this study aims to determine whether MGF24E promotes bone defect healing through MGF24E-increased angiogenesis and whether MGF24E has positive effects on angiogenesis in vitro. The roles of MGF24E on angiogenesis and the underlying mechanisms were investigated. The cell proliferation, migration, and tubulogenesis of the human vascular endothelial EA.hy926 cells co-treated with 2% serum and MGF24E were determined to assess angiogenesis in comparison with 100 ng/ml of vascular endothelial growth factor 165 (VEGF(165))-positive control or vehicle control (phosphate-buffered saline). MGF24E treatment (10 ng/ml) significantly promoted the biological processes of angiogenesis on EA.hy926 cells compared with the vehicle control. The suppression of vascular endothelial growth factor and angiopoietin-I expressions by 2% serum starvation was reversed by the addition of 10 ng/ml of MGF24E in 2% serum medium. This result suggests that MGF24E has a protective effect on angiogenesis. Moreover, the inhibition of ERK due to PD98050 pretreatment completely abolished and mostly blocked MGF24E-induced proliferation and migration, respectively, whereas the MGF24-induced tubulogenesis and the angiogenic factor expression were only partially inhibited. These new findings suggest that MGF24E promotes angiogenesis by enhancing the expression of angiogenic cytokines which involves the MAPK/ERK-signaling pathway.
    Preview · Article · Mar 2012 · Acta Biochimica et Biophysica Sinica

Publication Stats

415 Citations
128.13 Total Impact Points


  • 2003-2015
    • Chongqing University
      • • Department of Biorheological Science and Technology
      • • School of Bioengineering
      • • Key Laboratory for Biomechanics and Tissue Engineering under the State Ministry of Education
      Ch’ung-ch’ing-shih, Chongqing Shi, China
  • 2008
    • Government of the People's Republic of China
      Peping, Beijing, China