Frédéric Bonté

LVMH Recherche, Lutetia Parisorum, Île-de-France, France

Are you Frédéric Bonté?

Claim your profile

Publications (84)156.28 Total impact

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Vandaterosides are polar glucosyloxybenzyl eucomate derivatives found in Vanda teres (Orchidaceae), which display biological activities that slow the skin ageing process. In order to obtain larger quantities to allow us to go further in the bioassays, the hydroalcoholic extract of aerial parts (leaves and stems) of V. teres were fractionated by centrifugal partition chromatography, combining isocratic, gradient and dual elution modes. The first fractionation was performed on the extract maintained in the stationary phase as water saturated in butanol, while increasing the polarity of the mobile phase by changing the proportions of ethyl acetate/1-butanol/water, in order to obtain two enriched fractions. Vandateroside I was then purified by isocratic mode with ethyl acetate/ethanol/water (46:14:40), while vandateroside II was obtained by combining isocratic elution with ethyl acetate/isopropanol/water (30:20:50) followed by a multiple dual mode with ethyl acetate/ethanol/water (46:14:40). In this manner, hundreds of milligrams of vandateroside I and II were recovered from 10 g of V. teres extract. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Full-text · Article · Jun 2015 · Journal of Separation Science
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: (Myo)fibroblasts are key players for maintaining skin homeostasis and for orchestrating physiological tissue repair. (Myo)fibroblasts are embedded in a sophisticated extracellular matrix (ECM) that they secrete, and a complex and interactive dialogue exists between (myo)fibroblasts and their microenvironment. In addition to the secretion of the ECM, (myo)fibroblasts, by secreting matrix metalloproteinases and tissue inhibitors of metalloproteinases, are able to remodel this ECM. (Myo)fibroblasts and their microenvironment form an evolving network during tissue repair, with reciprocal actions leading to cell differentiation, proliferation, quiescence, or apoptosis, and actions on growth factor bioavailability by binding, sequestration, and activation. In addition, the (myo)fibroblast phenotype is regulated by mechanical stresses to which they are subjected and thus by mechanical signaling. In pathological situations (excessive scarring or fibrosis), or during aging, this dialogue between the (myo)fibroblasts and their microenvironment may be altered or disrupted, leading to repair defects or to injuries with damaged and/or cosmetic skin alterations such as wrinkle development. The intimate dialogue between the (myo)fibroblasts and their microenvironment therefore represents a fascinating domain that must be better understood in order not only to characterize new therapeutic targets and drugs able to prevent or treat pathological developments but also to interfere with skin alterations observed during normal aging or premature aging induced by a deleterious environment.
    Preview · Article · Nov 2014 · Clinical, Cosmetic and Investigational Dermatology

  • No preview · Article · Oct 2014 · Planta Medica
  • Source

    Full-text · Dataset · Oct 2014
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: IntroductionIn our continued efforts to contribute to the general knowledge on the chemical diversity of orchids, we have decided to focus our investigations on the Aeridinae subtribe. Following our previous phytochemical study of Vanda coerulea, which has led to the identification of phenanthrene derivatives, a closely related species, Aerides rosea Lodd. ex Lindl. & Paxton, was chosen for investigation.Objective To identify new secondary metabolites, and to avoid isolation of those already known, by means of the combined systems HPLC–DAD(diode-array detector) with high-resolution tandem mass spectrometry (HRMS/MS) and HPLC–DAD–MS–SPE(solid-phase extraction)–UV–NMR.MethodsA dereplication strategy was developed using a HPLC–DAD–HRMS/MS targeted method and applied to fractions from A. rosea stem extract. Characterisation of unknown minor compounds was then performed using the combined HPLC–DAD–MS–SPE–UV–NMR system.ResultsThe dereplication method allowed the characterisation of four compounds (gigantol, imbricatin, methoxycoelonin and coelonin), previously isolated from Vanda coerulea stem extract. The analyses of two fractions permitted the identification of five additional minor constituents including one phenanthropyran, two phenanthrene and two dihydrophenanthrene derivatives. The full set of NMR data of each compound was obtained from microgram quantities.Conclusion Nine secondary metabolites were characterised in A. rosea stems, utilising HPLC systems combined with high-resolution analytical systems. Two of them are newly described phenanthrene derivatives: aerosanthrene (5-methoxyphenanthrene-2,3,7-triol) and aerosin (3-methoxy-9,10-dihydro-2,5,7-phenanthrenetriol). Copyright © 2014 John Wiley & Sons, Ltd.
    Full-text · Article · Jul 2014 · Phytochemical Analysis
  • Frédéric Bonté · Bernard Kuballa · Annelise Lobstein
    [Show abstract] [Hide abstract]
    ABSTRACT: This article describes an unpublished correspondence between Augustin-Ambroise Delondre (1823- 1879), son of the famous pharmacist Augustin - Pierre Delondre and Friedrich August Flückiger, Swiss pharmacist (1828-1894), professor between 1873 to 1892 of the Chair in pharmacy at the university of Strasbourg and considered as the father of pharmacognosy. This set of 9 unique hand- written letters (1868 and 1869) allows to have an clearer idea of their scientific and human relations.
    No preview · Article · Jan 2014 · Revue d'histoire de la pharmacie
  • Frédéric Bonté · Alexis Desmoulière
    [Show abstract] [Hide abstract]
    ABSTRACT: Produced by bees, honey is a complex product, the fruit of the interactions between foraged flowers, the soil and metabolic systems linked to the genetic singularity of the bees. This mix of sugars, phenolic compounds, vitamins, amino acids, trace elements and specific molecules can bestow on it specific biological activities.
    No preview · Article · Dec 2013 · Actualités Pharmaceutiques
  • Source
    V Cakova · P Wehrung · P André · F Bonté · A Lobstein

    Full-text · Article · Aug 2013 · Planta Medica
  • Source

    Full-text · Article · Aug 2013 · Planta Medica
  • Source
    Marc Dumas · Frédéric Bonté · Alain Meybeck
    [Show abstract] [Hide abstract]
    ABSTRACT: Prunella vulgaris and Azadirachta indica extracts were shown to inhibit type I and II phospholipase A2 activity. These enzymes, involved in the biosynthesis of inflammation and allergy mediators and in the differentiation process of keratinocytes, are suitable for the screening of substances with a potent pharmacological activity.
    Preview · Article · Apr 2013 · Acta botanica Gallica: bulletin de la Société botanique de France
  • Source
    Frédéric Bonté · Marc Dumas · Patrick Barré · Alain Meybeck
    [Show abstract] [Hide abstract]
    ABSTRACT: Prunella vulgaris extracts have been shown to increase the keratinocytes differentiation in culture. The increase of the transglutaminase activity is one of the possible mecanisms involved in the activity.
    Preview · Article · Apr 2013 · Acta botanica Gallica: bulletin de la Société botanique de France

  • No preview · Article · Feb 2013 · Journal of Dermatological Science
  • Source
    V Cakova · A Urbain · A Sester · P André · F Bonté · A Lobstein

    Full-text · Article · Jul 2012 · Planta Medica
  • Source
    V Cakova · P Wehrung · A Urbain · P André · F Bonté · A Lobstein

    Full-text · Article · Jul 2012 · Planta Medica
  • Source
    V Cakova · P Wehrung · A Urbain · P André · F Bonté · A Lobstein

    Full-text · Article · Jul 2012 · Planta Medica
  • Frédéric Bonté
    [Show abstract] [Hide abstract]
    ABSTRACT: The 11(th) Annual LVMH Recherche Scientific Symposium was held in London on October 27(th), into the warmth of the distinguished British Library, with nearly 150 industry and research attendees. The meeting organized by LVMH Recherche was centered on the theme of skin rejuvenation. The current state of play for rejuvenation research was summarized, and then advances in the science of skin aging and rejuvenation therapies were discussed in detail. Personalized genomics and current and prospective translational therapies were presented, followed by a clever linking of multiple global theories towards a cohesive plan for future goals in rejuvenation research.
    No preview · Article · May 2012 · European journal of dermatology: EJD
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Since its first description in wound granulation tissue, the myofibroblast has been recognized to be a key actor in the epithelial-mesenchymal cross-talk that plays a crucial role in many physiological and pathological situations, such as regulation of prostate development, ventilation-perfusion in lung alveoli or organ fibrosis. The presence of myofibroblasts in the stroma reaction to epithelial tumors is well established and many data are accumulating which suggest that the stroma compartment is an active participant in tumor onset and/or evolution. In this review we summarize the evidence in favor of this concept, the main mechanisms that regulate myofibroblast differentiation and function, as well as the biophysical and biochemical factors possibly involved in epithelial-stroma interactions, using liver carcinoma as main model, in view of achieving a better understanding of tumor progression mechanisms and of tools directed toward stroma as eventual therapeutic target.
    Full-text · Article · May 2012 · Cell adhesion & migration
  • [Show abstract] [Hide abstract]
    ABSTRACT: The fibroblasts and the myofibroblasts are key players for maintaining skin homeostasis and for orchestrating physiological tissue repair. The (myo)fibroblasts are embedded in a sophisticated extracellular matrix (ECM) that they secrete, and a complex and interactive dialogue exists between (myo)fibroblasts and their microenvironment. The composition of the ECM around (myo)fibroblasts is variable depending on the situation and, in addition to the secretion of the ECM, the (myo)fibroblasts, by secreting matrix metalloproteinases and tissue inhibitors of metalloproteinases can remodel this ECM. The (myo)fibroblasts and their microenvironment form a changing network with reciprocal actions leading to cell differentiation, proliferation, quiescence or apoptosis, and also acting on growth factor biodisponibility. In pathological situations (such as chronic wounds or excessive scarring), or during ageing, especially due to ultraviolet exposition, this dialogue between the (myo)fibroblasts and their microenvironment is disrupted, leading to repair defects or to skin injuries with unaesthetic alterations such as wrinkles. Knowing the intimate exchanges between the (myo)fibroblasts and their microenvironment represents a fascinating domain important not only for characterizing new targets and drugs able to prevent pathological developments but also for interfering with skin alterations observed during ageing.
    No preview · Article · Nov 2011 · Pathologie Biologie
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Eucomic acid [(2R)-2-(p-hydroxybenzyl)malic acid)] (1) and three new glucopyranosyloxybenzyl eucomate derivatives, vandaterosides I (2), II (3), and III (4), were isolated and identified from the stems of Vanda teres. Their cellular antiaging properties were evaluated in a human immortalized keratinocyte cell line (HaCaT) by monitoring their effect on cytochrome c oxidase activity, implicated in mitochondrial respiratory function and cellular energy production. Eucomic acid (1) and vandateroside II (3) increased cytochrome c oxidase activity and/or expression, without enhancing cellular mitochondrial content. These two V. teres biomarkers apparently contributed to stimulate respiratory functions in keratinocytes. Since aging and its pathologies may be ascribed to a decline in mitochondrial functions, these biomarkers have the potential to become new natural ingredients for antiaging preparations to remedy age-related disorders such as skin aging.
    Full-text · Article · May 2011 · Journal of Natural Products
  • F Bonté
    [Show abstract] [Hide abstract]
    ABSTRACT: The main function of the skin is to protect the body against exogenous substances and excessive water loss. The skin barrier is located in the outermost layer of the skin, called the stratum corneum, which is composed of corneocytes, originating from the keratinocytes differentiation process, embedded in organized complex lipid domains. Moisturizing of the skin is recognized as the first anti-aging skin care. Skin moisturization is essential for its appearance, protection, complexion, softness and the reinforcement of its barrier properties against deleterious and exogenous environmental factors. The intrinsic water binding capacity of skin is not only due to the complex natural moisturizing factor present in corneocytes, but also to hyaluronic acid and a regulated water transport within the skin. Recent data shows that the water movements between the cells at the different levels of the epidermis are due to dedicated water and glycerol transport proteins named aquaporins. Their role in the skin moisturization is completed by corneodesmosomes and tight junctions. Water and pH are now shown to be of prime importance in the regulation of the epidermal enzymes linked to corneocytes desquamation and lipid synthesis. Furthermore, the level of moisturization of the skin is important in its protection against repeated exposure to various irritant agents or phenomena such as very frequent washing with strong tensioactive materials.
    No preview · Article · May 2011 · Annales Pharmaceutiques Françaises

Publication Stats

1k Citations
156.28 Total Impact Points

Institutions

  • 1999-2015
    • LVMH Recherche
      Lutetia Parisorum, Île-de-France, France
  • 2008
    • Université Paris-Sud 11
      Orsay, Île-de-France, France
    • Università degli Studi di Modena e Reggio Emilia
      • Department of Biomedical, Metabolical and Neurosciences
      Modène, Emilia-Romagna, Italy
  • 2005
    • Charité Universitätsmedizin Berlin
      Berlín, Berlin, Germany
  • 2004
    • Istituto Dermopatico dell'Immacolata
      Roma, Latium, Italy
  • 2002-2003
    • Claude Bernard University Lyon 1
      Villeurbanne, Rhône-Alpes, France
  • 1994
    • Harvard Medical School
      Boston, Massachusetts, United States