[Show abstract][Hide abstract] ABSTRACT: Protein phosphorylation analysis is an enormous challenge. This review summarises the currently used techniques, which are based on radiolabelling and mass spectrometry as well as electrophoretic and chromatographic separation. Many methods exist, but there is still no single procedure applicable to all phosphoproteins. MS is able to deliver information about the location of phosphorylation sites, but phosphospecific properties with respect to ionisation present obstacles. Therefore, multidimensional approaches involving several analytical methods are often necessary to conquer phosphorylation site identification.
Preview · Article · Mar 2004 · Analytical and Bioanalytical Chemistry
[Show abstract][Hide abstract] ABSTRACT: Radioactive assays are commonly employed to monitor protein or peptide phosphorylation. They not only have all the disadvantages related to radioactivity, but also require large amounts of sample. An alternative is the use of mass spectrometric peptide mapping with sensitivities in the fmole range. We demonstrate here that desalting is a requirement for reproducible results, and we optimized the method for very hydrophilic peptide substrates. The method is very efficient with respect to time and effort.
No preview · Article · Mar 2003 · Journal of Experimental Therapeutics and Oncology
[Show abstract][Hide abstract] ABSTRACT: A long-chain derivative of 1,3-dioxolane sodium propyloxy sulfate, with similar denaturing and electrophoretic properties as SDS, and facilitated protein identification following polyacrylamide gel electrophoresis (PAGE) for Coomassie-stained protein bands, has been tested. Comparative acid-labile surfactant/sodium dodecyl sulfate two-dimensional (ALS/SDS 2-D)-PAGE experiments of lower abundant proteins from the proteomes of regenerating rat retina and mouse brain show that peptide recovery for mass spectrometry (MS) mapping is significantly enhanced using ALS leading to more successful database searches. ALS may influence some procedures in proteomic analysis such as the determination of protein content and methods need to be adjusted to that effect. The promising results of the use of ALS in bioanalytics call for detailed physicochemical investigations of surfactant properties.
[Show abstract][Hide abstract] ABSTRACT: Modern protein identification and analysis relies largely on proteolytic in-gel digestion of proteins separated during polyacrylamide gel electrophoresis (PAGE) followed by mass spectrometric (MS) measurement of the extracted peptides. Sodium dodecyl sulfate (SDS) is routinely used in nonnative PAGE. However, SDS can interfere with MS. We report the use of an acid-labile surfactant (ALS-I) in place of SDS. ALSI is a long chain derivative of 1,3-dioxolane sodium propyloxy sulfate and has similar denaturing and electrophoretic properties as SDS, but it decomposes at low pH and enhances MS detection of proteins.
[Show abstract][Hide abstract] ABSTRACT: In this paper, analysis strategies developed for a sequencing problem concerning the identification of an S100 protein isolated from human granulocytes are discussed. The analysis of a trypsinized lyophilized sample suggested the presence of a number of peptides which are non-tryptic in origin. During purification of proteins from cell lysates nonspecific cleavage can be observed which may reflect biological processes and can become an unavoidable analytical problem. Current mass spectrometric software is evaluated for the analysis of nonspecific digests in this context. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), high-performance liquid chromatography (HPLC)-MS/MS, and selected ion monitoring (SIM)-MS/MS have been used for peptide analysis and in addition HPLC-MS was carried out for protein analysis leading to the detection of an N-terminal modification of the protein. The success of the study is mainly due to the careful investigation of nonspecific cleavage products. Data obtained from the routine mass spectrometric analysis of an in-gel-digest allowed the identification of this protein as S100 calcium-binding protein A6-calcyclin whose expression in granulocytes has not been described so far.
Full-text · Article · Dec 2001 · Journal of the American Society for Mass Spectrometry