[Show abstract][Hide abstract] ABSTRACT: Background:
Human brucellosis is a preventable zoonoses that may become persistent, causing, if left untreated, severe localized disease. Occupational exposure to infected animals or animal products and consumption of fresh contaminated dairy are main risk factors.
One hundred farmworkers employed at two cattle farms one in Khartoum North and one in Omdurman were screened for the presence of specific antibodies and seropositive workers were invited to donate a blood sample for blood culture. Molecular typing was used to characterize Brucella isolates.
Ten percent of farmworkers tested seropositive and while Brucella melitensis biovar 1 was isolated from the blood of three individuals, an isolate identical to the B. abortus S19 vaccine strain was isolated from a fourth person. All four bacteremic individuals were employed as milkers and did not have obvious disease.
The isolation of the highly infectious pathogen B. melitensis from seropositive workers is consistent with the notion that the pathogen may persist in the blood without causing overt disease. While vaccination with strain S19 is essential for the control of bovine brucellosis the vaccine strain may be transmitted to the human population and protective measures remain important to prevent exposure also in view of the presence of B. melitensis. To create awareness for this potentially severe disease more information on the prevalence of the pathogen in different risk groups and in livestock in the Sudan is needed.
[Show abstract][Hide abstract] ABSTRACT: Brucellosis is a major cause of infertility and reproductive failure in livestock. While cattle in the Eastern Indonesian archipelago suffers from reproductive problems information on bovine brucellosis in the region is fragmentary. The control of brucellosis requires a major and prolonged effort and confirmation of the infection by isolation with detailed knowledge of the spread of the infection is essential when planning a control program.
Serological investigation of Brucella infection in beef cattle tended under extensive farming conditions revealed a high seroprevalence (19.3%; 95%CI, 17--22) in the compliment fixation tests. The results of a rapid and simple field test correlated well with the Rose Bengal test (kappa, 0.917) and indicated an acceptable sensitivity (87.5%) and specificity (98.1%) compared with the complement fixation test. Reproductive failure was reported for 39.0% of the cows with a loss of calves due to abortion or early death amounting to 19.3%. Past reproductive failure did not, however, correlate with seropositivity in the complement fixation test (RP = 1.21; P = 0.847). B. abortus biovar 1 was freshly isolated from the hygromas of two cows and together with thirty banked isolates collected since 1990 from different parts of Sulawesi and Timor eight related genotypes could be distinguished with one genotype being identical to that of an isolate (BfR91) from Switzerland. The Indonesian genotypes formed together with BfR91 and one African and one North American isolate a distinct branch on the B. abortus biovar 1 dendogram.
Bovine brucellosis appears to be widespread in the Eastern Indonesian archipelago and calls for urgent intervention. The fresh isolation of the pathogen together with the observed high seroprevalence demonstrates the presence and frequent exposure of cattle in the area to the pathogen. The application of a rapid and simple field test for brucellosis could be very useful for the quick screening of cattle at the pen side.
Full-text · Article · Nov 2013 · BMC Veterinary Research
[Show abstract][Hide abstract] ABSTRACT: The traditional lifestyle and beliefs of pastoralists and small-scale farmers with confined livestock, together with certain farming environments, create favourable conditions for the spread and transmission of brucellosis. The risks associated with these practices are difficult to control because of a lack of alternatives and simple and/or affordable solutions. Brucellosis affects the health and productivity of livestock as well as that of their owners and caretakers and can have a deep economic impact. The control of brucellosis is likely to be cost effective. Good quantitative information on brucellosis in livestock and the human population is essential for demonstrating the benefits of intervention. Effective vaccines for the control of brucellosis in cattle and small ruminants are available and cheap, and in high-risk areas there is an urgent need to start large-scale vaccination programmes. Risks for the spread and transmission of brucellosis, such as the migration of herds with frequent contacts with other herds at common feeding grounds and near water sources, are inherent in the way of life of pastoralists. Such risks may need to be accepted when developing a control programme. Thus, the control of brucellosis by vaccination is expected to be more effective for confined livestock. Essential to the success of mass vaccination in controlling brucellosis is achieving a high degree of protection of adult livestock in a very short period and vaccinating young stock before natural infection can occur. To reduce the risk of transmission of infection from neighbouring areas where animals are not vaccinated, a region-wide approach is important. Because shepherds and farmers may have very little knowledge of infectious diseases and the consequences of infection, providing disease information and education is important to help them understand the need for control measures. Public health services can also assist in encouraging acceptance of control programmes in livestock by creating awareness of brucellosis as a human disease. To reduce costs, brucellosis control programmes can be combined with other veterinary or public health activities or interventions. An up-to-date livestock census and an effective surveillance system are crucial for the control of brucellosis, as the disease may quickly re-emerge from remaining foci of infection. Although test and slaughter may be an option for the management of remaining or re-emerging foci of infection, such a strategy is frequently not an option because of the cost.
No preview · Article · Apr 2013 · Revue scientifique et technique (International Office of Epizootics)
[Show abstract][Hide abstract] ABSTRACT: Evaluation of: Siba V, Horwood PF, Vanuga K et al. Evaluation of serological diagnostic tests for typhoid fever in Papua New Guinea using a composite reference standard. Clin. Vaccine Immunol. 19(11), 1833-1837 (2012). The study under review evaluated serological tests for typhoid fever against PCR as a reference test. While laboratory testing is essential for the confirmation of this severe disease, the low bacterial load and the low level of specific antibodies in the blood of typhoid patients combined with its acute character make interpretation of laboratory testing cumbersome. Validation of an index test requires good understanding of the diagnostic performance and assay characteristics of the reference test, and criteria and principles for study design and reporting outlined by the Quality Assessment of Diagnostic Accuracy Studies and the Standards for Reporting Studies of Diagnostic Accuracy should be followed. Described PCR assays for typhoid fever have not been validated against bone marrow culture, the gold standard, and their diagnostic utility remains to be established.
No preview · Article · Mar 2013 · Expert Review of Molecular Diagnostics
[Show abstract][Hide abstract] ABSTRACT: Brucella melitensis is highly infectious for humans and can be transmitted to humans in a number of epidemiological contexts. Within the context of an ongoing brucellosis surveillance project, an outbreak at a Peruvian police officer cafeteria was discovered, which led to active surveillance (serology, blood culture) for additional cases among 49 police officers who had also eaten there. The cohort was followed up to 18 months regardless of treatment or symptoms. Active surveillance estimated the attack rate at 26.5% (13 of 49). Blood cultures from four cases were positive; these isolates were indistinguishable using multiple locus variable number tandem repeat analysis. This investigation indicates the importance of case tracking and active surveillance for brucellosis in the context of potential common source exposure. These results provide rationale for public health investigations of brucellosis index cases including the bioterrorism-related dissemination of Brucella.
Full-text · Article · Feb 2013 · The American journal of tropical medicine and hygiene
[Show abstract][Hide abstract] ABSTRACT: Nigeria is the largest cattle-rearing nation in Africa with most animals kept under traditional husbandry practices. While bovine brucellosis does not receive much attention, a relatively high seroprevalence is found in samples submitted for laboratory testing. The aim of the study was to provide serological evidence of brucellosis in cattle from some of the main cattle-rearing states of the country and to validate a simple and rapid field test for the serodiagnosis of bovine brucellosis.
Serum samples collected in various states of Nigeria from cattle because of suspicion of brucellosis were investigated in the Rose Bengal plate test, and results were compared with a newly developed rapid field test for the detection of Brucella-specific antibodies.
Serological evidence for the presence of brucellosis in cattle was obtained for all states included in the study and a high herd prevalence was observed. The seroprevalence was also high among trade and slaughter animals. Results of a rapid field test for the serodiagnosis of bovine brucellosis correlated well with the Rose Bengal plate test (agreement, 95.7%; kappa value, 0.80).
The results indicate that bovine brucellosis is an important veterinarian problem in Nigeria. The easy-to-use and robust field test is most promising for field-based surveillance as it provides an immediate result allowing the prompt instigation of control measures.
Full-text · Article · Nov 2011 · Tropical Animal Health and Production
[Show abstract][Hide abstract] ABSTRACT: Multi-locus variable-number tandem repeat analysis differentiated 297 Salmonella enterica serovar Typhi blood culture isolates from Makassar in 76 genotypes and a single unique S. Typhi genotype was isolated from the cholecystectomy specimens of four patients with cholelithiasis. The high diversity in S. Typhi genotypes circulating in Makassar indicates that the number of carriers could be very large, which may complicate disease prevention and control.
[Show abstract][Hide abstract] ABSTRACT: There is an urgent need for affordable point-of-care diagnostics for the differentiation of febrile illnesses and the confirmation of typhoid in endemic countries.
Blood samples were collected from febrile patients with clinical suspicion of typhoid and screened for typhoid fever using the Widal and Typhi Dri Dot tests, while stool and blood samples were screened for Salmonella Typhi using the culture method as well as PCR as a confirmatory test.
A high proportion of febrile patients from Lagos with clinical suspicion of typhoid fever reacted positively in a simple and rapid latex agglutination assay for typhoid fever, indicating that this illness is a common and presumably under-diagnosed health problem in this metropolis. Seropositivity was 19.2% in the rapid test compared with 22.9% in the classical Widal test. The confirmation of typhoid in these seropositive patients appeared cumbersome because of negative blood cultures and low DNA yield in molecular testing. A review of the literature revealed that in Nigeria seroprevalence rates can be high in the normal population and that pathogens other than S. Typhi are often isolated from the blood of seropositive febrile patients.
The simplicity and the relatively high specificity (97.8%) of the rapid test as determined in a study performed in Indonesia calls for a further validation of this promising test for use in Africa.
Full-text · Article · Jul 2011 · The Journal of Infection in Developing Countries
[Show abstract][Hide abstract] ABSTRACT: Brucella melitensis biovar 1 was isolated from bovine milk samples from a herd in central Kenya, and Brucella abortus biovar 3 was isolated from aborted fetus materials and vaginal discharge fluids from cattle in central and eastern provinces of Kenya. All infections including those with B. melitensis were in cattle with reproductive problems kept in mixed herds indicating that cross infection occurs from small ruminants. Multiple-locus variable-number tandem repeat analysis genotyping revealed a close molecular homology of the B. melitensis isolates with an isolate from Israel and a close homology of the B. abortus isolates with an isolate from Uganda indicating that these genotypes have a wide geographic distribution. Infection of cattle with B. melitensis may complicate the control of brucellosis in this country.
No preview · Article · Jun 2011 · Tropical Animal Health and Production
[Show abstract][Hide abstract] ABSTRACT: Phase variation is a property unique of some Salmonella enterica serovar Typhi strains from Indonesia. Salmonella Typhi isolates from Indonesia have been described that in addition to the phase 1 Hd flagellin gene contain a second flagellin gene named z66. S. Typhi isolates from Indonesia with a mutant Hd gene named Hj have also been described. Here, we have identified another flagellin gene of S. Typhi, named Ind, showing a closest homology with the flagellin gene of Serratia marcescens. The Ind gene was detected in 21.8% of the S. Typhi isolates from the East Indonesian archipelago, all of which contained the Hd gene. The Hj gene was not detected. The z66 gene was present in 15.4% of the isolates. The presence of these "foreign" flagellin genes could be associated with an increased risk for developing severe disease.
Full-text · Article · Mar 2011 · The American journal of tropical medicine and hygiene
[Show abstract][Hide abstract] ABSTRACT: Background: Micronutrients such as zinc (Zn) and copper (Cu) have a modulatory effect on immune system. Altered serum concentrations of these nutrients have been described in patients with specific disease conditions. The aim of this study was to evaluate serum Zn and Cu level alterations in patients with brucellosis in comparison with healthy individuals. Patients and methods: Serum Zn and Cu level of 43 patients with brucellosis (34 men and 9 women) were compared with 43 matched healthy controls. Serum micronutrient concentrations were measured by automatic absorptive spectrophotometry. Results: Mean serum Cu concentration was significantly higher in subjects with brucellosis when compared with age-matched healthy controls (p<0.05). Mean serum Zn level was decreased in female patients compared with controls (p<0.05), however, there was no significant difference between male patients and controls. Conclusion: Serum Zn and Cu concentrations may alter in patients with brucellosis during the period of infection. Further studies are needed to determine whether these micronutrients have an effect on disease severity and outcome. Measuring serum Cu level may be suggested as a complementary screening tool for brucellosis.
Full-text · Article · Jan 2010 · Iranian Journal of Clinical Infectious Diseases
[Show abstract][Hide abstract] ABSTRACT: Serum samples from all patients with culture-confirmed brucellosis including those with chronic disease from Kazakhstan tested positive in the serum agglutination test for titers > or = 1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays. The strong reactivity in the LFA observed for the majority (92.1%) of the samples from the patients with culture-confirmed brucellosis together with the user-friendliness of the assay procedure makes the LFA ideal for the confirmation of brucellosis in endemic areas in Kazakhstan. The Rose Bengal test lacked sensitivity in particular for patients with chronic brucellosis therefore limiting its value as a quick screening assay. The study emphasizes the importance of the LFA as a useful, rapid, and easy-to-perform tool in the diagnostic testing of brucellosis.
Full-text · Article · Oct 2009 · Diagnostic microbiology and infectious disease
[Show abstract][Hide abstract] ABSTRACT: The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of
16 loci investigated, of which 1 (Bruce42) also is used for species identification. Ten genotypes were identified, separated
by the number of Bruce42 repeats into two groups that may have distinct phenotypic characteristics. Whereas genotypes with
five or six Bruce42 repeats were cultured mainly from adult patients, genotypes with three Bruce42 repeats were isolated from
children and young adolescents as well as from adults. In addition, the isolates with three Bruce42 repeats were obtained
more often from patients with splenomegaly (P = 0.02) or hepatomegaly (P = 0.006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh
dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.
[Show abstract][Hide abstract] ABSTRACT: The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier than the Ruiz-Castaneda method. This lysis method is inexpensive and easier to use and may be used in laboratories with limited expertise or equipment if all safety precautions are taken.
No preview · Article · May 2009 · The American journal of tropical medicine and hygiene
[Show abstract][Hide abstract] ABSTRACT: The prospects for the control of neglected tropical diseases, including soil-transmitted helminthiasis, shistosomiasis, lymphatic filariasis, onchocerciasis and trachoma, through mass drug administration, are exemplified by the elimination of the trachoma as a public-health problem in Morocco. In spite of this and other striking successes, mass drug administration programs are faced with major challenges resulting from suboptimal coverage and lack of efficacy. At current suboptimal coverage rates, programs may need prolongation for an extended period, increasing costs and undermining sustainability. Community participation through health education and information appears to be crucial to improve coverage and to achieve sustainability. Implementation of complementary measures, such as vector control, improved hygiene and environmental sanitation, are important to further control transmission and to prevent re-emergence of the infection and, again, may only be achieved effectively through community-based initiatives. To reduce costs and to relieve pressure on the health system, combining neglected tropical disease programs in areas where diseases coexist and integration with existing control programs for malaria, tuberculosis and HIV/AIDS is advocated. The risk of developing drug resistance is of particular concern in view of the lack of alternative drugs, and reduced treatment efficacy due to emerging resistance is evident for the soil-transmitted helminths and onchocerciasis. Given the risk for the development of drug resistance and the need for a high degree of participation, close attention should be paid to the monitoring of the coverage and efficacy of the different program components.
Preview · Article · Mar 2009 · Expert Review of Anti-infective Therapy
[Show abstract][Hide abstract] ABSTRACT: Brucellosis is considered by the Food and Agricultural Organisation and the World Health Organisation as one of the most widespread zoonoses in the world. It is a major veterinary public health challenge as animals are almost exclusively the source of infection for people. It is often undiagnosed in both human patients and the animal sources and it is widely acknowledged that the epidemiology of brucellosis in humans and animals is poorly understood, particularly in sub-Saharan Africa. It is therefore important to develop better diagnostic tools in order to improve our understanding of the epidemiology and also for use in the field for disease control and eradication. As with any new diagnostic test, it is essential that it is validated in as many populations as possible in order to characterise its performance and improve the interpretation of its results. This paper describes a comparison between a new lateral flow assasy (LFA) for bovine brucellosis and the widely used cELISA in a no gold standard analysis to estimate test performance in this West African cattle population. A Bayesian formulation of the Hui-Walter latent class model incorporated previous studies' data on sensitivity and specificity of the cELISA. The results indicate that the new LFA is very sensitive (approximately 87%) and highly specific (approximately 97%). The analysis also suggests that the current cut-off of the cELSIA may not be optimal for this cattle population but alternative cut-offs did not significantly change the estimates of the LFA. This study demonstrates the potential usefulness of this simple to use test in field based surveillance and control which could be easily adopted for use in developing countries with only basic laboratory facilities.
[Show abstract][Hide abstract] ABSTRACT: Recent human Brucella melitensis isolates from Peru were genotyped by multiple locus variable number repeat analysis. All 24 isolates originated from hospitalized patients living in the central part of Peru and consisted of six genomic groups comprising two to four isolates and nine unique genotypes. The isolates were most closely related to the two previously genotyped isolates from Mexico, with a maximum distance of 2 to 4. The Peruvian strains were clearly distinct from the East and West Mediterranean groups of B. melitensis genotypes, suggesting that they may constitute a unique Latin American cluster.
No preview · Article · Dec 2008 · Transactions of the Royal Society of Tropical Medicine and Hygiene