Zhi-Guo Shi

Wuhan University, Wu-han-shih, Hubei, China

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Publications (66)225.96 Total impact

  • Huan Sun · Qiong-Wei Yu · Haibo He · Qian Lu · Zhi-Guo Shi · Yu-Qi Feng
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    ABSTRACT: A novel nickel oxide nanoparticles-deposited silica composite (SiO2@NiO) was prepared via liquid phase deposition (LPD) and then employed as a solid phase extraction (SPE) sorbent. By coupling the SPE with liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS) analysis, an analytical platform for the sensitive determination of benzimidazole residues in egg and milk was established. The limits of detection of nine benzimidazoles were in the range of 0.8-2.2 ng/mL in milk and 0.3-2.1 ng/g in eggs, respectively, which was 5 to 10 times superior to the methods with other adsorbents for SPE. The recoveries of nine benzimidazoles spiked in milk and egg ranged from 70.8 to 118.7%, with relative standard deviations (RSDs) being less than 18.9%. This work presented the excellent extraction performance of NiO on benzimidazoles for the first time, and the applicability of LPD technique used as sorbents for trace analysis in complex matrices was also demonstrated.
    No preview · Article · Dec 2015 · Journal of Agricultural and Food Chemistry
  • Liyun Ma · Jing Li · Juan Zhao · Han Liao · Li Xu · Zhi-guo Shi
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    ABSTRACT: In the current study, novel featured silica, named penetrable silica, simultaneously containing macropores and mesopores, was immobilized with bovine serum albumin (BSA) via Schiff base method. The obtained BSA-SiO2 was employed as the high-performance liquid chromatographic (HPLC) stationary phase. Firstly, d- and l-tryptophan were used as probes to investigate the chiral separation ability of the BSA-SiO2 stationary phase. An excellent enantioseparation factor was obtained up to 4.3 with acceptable stability within at least 1 month. Next, the BSA-SiO2 stationary phase was applied to study the interaction between imatinib mesylate (IM) and BSA by frontal affinity chromatography. A single type of binding site was found for IM with the immobilized BSA, and the hydrogen-bonding and van der Waals interactions were expected to be contributing interactions based on the thermodynamic studies, and this was a spontaneous process. Compared to the traditional silica for HPLC stationary phase, the proposed penetrable silica microsphere possessed a larger capacity to bond more BSA, minimizing column overloading effects and enhancing enantioseparation ability. In addition, the lower running column back pressure and fast mass transfer were meaningful for the column stability and lifetime. It was a good substrate to immobilize biomolecules for fast chiral resolution and screening drug-protein interactions. Graphical Abstract The preparation of BSA-SiO2 column based on the penetrable silica and its application in chiral separation and frontal analysis
    No preview · Article · Nov 2015 · Analytical and Bioanalytical Chemistry
  • Zhi-Yuan Luo · Hai-Yan Liu · Zhi-Guo Shi
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    ABSTRACT: In the present study, a novel configuration of liquid-phase microextraction was proposed, in which a magnetic stirrer with a groove was used as the extractant phase holder. It was termed as magnetic stirrer liquid-phase microextraction. In this way, the stability of the organic solvent gained much improved under high stirring speed; the extraction efficiency was enhanced due to the enormously enlarged contact area between the organic solvent and aqueous phase. The extraction performance of the magnetic stirrer liquid-phase microextraction was studied using chlorobenzenes as the probe analytes. A wide linearity range (20 pg/mL to 200 ng/mL) with a satisfactory linearity coefficient (r(2) > 0.998) was obtained. Limits of detection ranged from 9.0 to 12.0 pg/mL. Good reproducibility was achieved with intra-day and inter-day relative standard deviations <4.8%. The proposed magnetic stirrer liquid-phase microextraction was simple, environmentally friendly and efficient; compared to single-drop microextraction, it had obvious advantages in terms of reproducibility and extraction efficiency. It is a promising miniaturized liquid-phase technology for real applications. This article is protected by copyright. All rights reserved.
    No preview · Article · Nov 2015 · Journal of Separation Science
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    ABSTRACT: Magnetic nanoparticles (MNPs), prepared via thiol-ene click chemistry and containing both diol and octadecyl groups, are shown to possess both hydrophobic and hydrophilic functionalities. They display excellent dispersibility in water and also are capable of extracting non-steroidal anti-inflammatory drugs (NSAIDs) from water samples. The MNPs can be magnetically separated, and the NSAIDs eluted with acetonitrile-water (9:1, v:v) and submitted to high performance liquid chromatographic analysis. Extraction variables, such as the kind of ion-pairing reagents, amount of MNPs, pH of sample solution, extraction and desorption time, volume of desorption solvent and salt addition, were optimized. Under optimum conditions, the method has a wide analytical range (from 5 to 800 ng∙mL‾1), good reproducibility with intra-day and inter-day relative standard deviations of <19.2 % (for n = 6), and low detection limits of 0.32 to 1.44 ng∙mL‾1 for water samples. The results demonstrate that the material possesses good water compatibility, thus warranting ease of operation and good reproducibility. [Figure not available: see fulltext.]
    No preview · Article · Sep 2015 · Microchimica Acta
  • Jian Li · Li Xu · Zhi-guo Shi · Min Hu
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    ABSTRACT: In this study, a novel two-dimensional liquid chromatographic (2D-LC) system was developed for simultaneous separation and toxicity prediction of pharmaceutical and its related substances. A conventional ODS column was used on the 1st-D to separate the sample; while, bio-partitioning micellar chromatography served as the 2nd-D to predict toxicity of the components. The established system was tested for the toxicity of ibuprofen and its impurities with known toxicity. With only one injection, ibuprofen and its impurities were separated on the 1st-D; and LC50 values of individual impurity were obtained based on the quantitative retention-activity relationships, which agreed well with the reported data. Furthermore, LC50 values of photolysis transformation products (TPs) of carprofen, ketoprofen and diclofenac acid (as unknown compounds) were screened in this 2D-LC system, which could be an indicator of the toxicity of these TPs and was meaningful for the environmental monitoring and drinking water treatment. The established 2D-LC system was cost-effective, time-saving and reliable, and was promising for fast online screening of toxicity of known and unknown analytes in the complex sample in a single step. It may find applications in environment, pharmaceutical and food, etc. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · Aug 2015 · Journal of hazardous materials
  • Qing Wang · Mao Ye · Li Xu · Zhi-Guo Shi
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    ABSTRACT: A mixed-mode chromatographic packing material, C18 and diol groups modified silica (C18-Diol), was prepared with controllable hydrophobicity and hydrophilicity. It demonstrated excellent aqueous compatibility and stability in aqueous mobile phase; compared to the traditional C18 column, improved peak shape of basic analytes was also obtained. Additionally, it exhibited both reversed-phase liquid chromatographic (RPLC) and hydrophilic interaction chromatographic (HILIC) performance; the analyte separation scope was thus enlarged, demonstrated by simultaneous separation of twenty acids, bases and neutrals. More interestingly, a novel on-line two-dimensional liquid chromatography on the single column (2D-LC-1C) was established by modifying the high performance liquid chromatographic instrument only with the addition of an extra six-port two-position valve. The early co-eluted components of the extract of Lonicera japonica on the 1st-dimension (RPLC) were collected for the online re-injection to the 2nd-dimension (HILIC) by conveniently varying the mobile phase components. Six more peaks were obtained. The established system was simple, easy operation and low cost, which had advantages in analyzing complicated samples. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · Aug 2015 · Analytica chimica acta
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    ABSTRACT: The present study described the preparation and application of zwitterionic stationary phases (ACS) with controllable ratio of positively charged tertiary amine groups and negatively charged carboxyl groups. Various parameters, including water content, pH values and ionic strength of the mobile phase, were investigated to study the chromatographic characteristics of ACS columns. The prepared ACS columns demonstrated a mix-mode retention mechanism composed of surface adsorption, partitioning and electrostatic interactions. The elemental analysis of different batches of the ACS phases demonstrated good reproducibility of the preparation strategy. Additionally, various categories of compounds, including nucleosides, water-soluble vitamins, benzoic acid derivatives and basic compounds were successively employed to evaluate the separation selectivity of the prepared ACS stationary phases. These ACS phases exhibited entirely different selectivity and retention behavior from each other for various polar analytes, demonstrating the excellent application potential in the analysis of polar compounds in HILIC. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Aug 2015 · Talanta
  • Shu-Wen Xue · Min-Qiong Tang · Li Xu · Zhi-Guo Shi
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    ABSTRACT: Magnetic nanoparticles (MNPs) featured with divinylbenzene (DVB) and sulfonate functionalities (Fe3O4-DVB-SO3(-)) were prepared via "thiol-ene" click chemistry. The hydrophobic DVB moieties were dedicated for extraction while the hydrophilic sulfonate groups were designed for dispersing the MNPs in aqueous sample solution. Thus, the specially designed material could ensure operational convenience and improve reproducibility during extraction. The application of the material was demonstrated by the extraction of polycyclic aromatic hydrocarbons (PAHs) from environmental water samples followed by gas chromatography-mass spectrometric analysis. The main factors influencing the extraction, including the type of the desorption solvent, the agitation mode, the amount of MNPs, extraction and desorption time and salt addition in sample solution, were investigated in detail. Under the optimized conditions, the proposed method showed satisfactory reproducibility with intra-day and inter-day relative standard deviations less than 16.5% and 21.2%, and low limits of detection of 1.1pgmL(-1), 0.8pgmL(-1), 1.1pgmL(-1), 1.4pgmL(-1), 0.6pgmL(-1), 2.1pgmL(-1) and 0.7pgmL(-1) for naphthalene, acenaphthene, fluorine, phenanthrene, anthracene, fluoranthene and pyrene, respectively. The developed method was also successfully used for determination of the PAHs in genuine lake and river environmental water samples by standard addition method. All the studied PAHs were detected in these waters with comparable results by the standard liquid-liquid extraction method. The developed MNPs with dual property of hydrophobicity and hydrophilicity were suitable for the treatment of water samples. The magnetic solid phase extraction based on this material was reliable and convenient. It has great potential in the preconcentration of trace analytes in complex matrix. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · Aug 2015 · Journal of Chromatography A
  • Jian Li · Li-Yun Ma · Li Xu · Zhi-Guo Shi
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    ABSTRACT: Benzophenone-type UV filters (BPs) are ubiquitous in the environment. Transformation products (TPs) of BPs with suspected toxicity are likely to be produced during disinfection of water by chlorination. To quickly predict the toxicity of TPs, in this study, a novel two-dimensional liquid-chromatography (2D-LC) method was established in which the objective of the first dimension was to separate the multiple components of the BPs sample after chlorination, using a reversed-phase liquid-chromatography mode. A biochromatographic system, i.e. bio-partitioning micellar chromatography with the polyoxyethylene (23) lauryl ether aqueous solution as the mobile phase, served as the second dimension to predict the toxicity of the fraction from the first dimension on the basis of the quantitative retention–activity relationships (QRARs) model. Six BPs, namely 2,4-dihydroxybenzophenone, oxybenzone, 4-hydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone and 2,2'-dihydroxy-4-methoxybenzophenone, were the target analytes subjected to chlorination. The products of these BPs after chlorination were directly injected to the 2D-LC system for analysis. The results indicated that most TPs may be less toxic than their parent chemicals, but some may be more toxic, and that intestinal toxicity of TPs may be more obvious than blood toxicity. The proposed method is time-saving, high-throughput, and reliable, and has great potential for predicting toxicity or bioactivity of unknown and/or known components in a complex sample. Graphical Abstract The scheme for the 2D-LC online prediction of toxicity of the transformation products of benzophenone-type UV filters after chlorination
    No preview · Article · Jun 2015 · Analytical and Bioanalytical Chemistry
  • Qing Wang · Zhi-Yuan Luo · Mao Ye · Yu-Zhuo Wang · Li Xu · Zhi-guo Shi
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    ABSTRACT: The zirconia-coated silica (ZrO2/SiO2) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol-gel technology, to take dual advantages of the suitable porous structure of SiO2 and basic resistance of ZrO2. Adenosine 5′-monophosphate (5′-AMP) was then self-assembled onto ZrO2/SiO2 via Lewis acid-base interaction, generating 5′-AMP-ZrO2/SiO2. The chromatographic properties of 5′-AMP-ZrO2/SiO2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5′-AMP-ZrO2/SiO2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO2/SiO2 and 5′-AMP-ZrO2/SiO2 was superior to the bare ZrO2, and different selectivity was obtained after the introduction of 5′-AMP. For acidic analytes, good resolution was obtained on 5′-AMP-ZrO2/SiO2 while the analysis failed on the bare ZrO2 column owing to strong adsorption. Hence, the proposed 5′-AMP-ZrO2/SiO2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO2 based SP.
    No preview · Article · Jan 2015 · Journal of Chromatography A
  • Qing Wang · Jie Chen · Kun Huang · Xin Zhang · Li Xu · Zhi-Guo Shi
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    ABSTRACT: A hydrophilic stationary phase (SP) was prepared through grafting N-methylene phosphonic acid chitosan on magnesia-zirconia particles (P-CTS-MgO-ZrO2) via Lewis acid-base interaction. The resulting material was characterized by thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscope and nitrogen adsorption analysis. The chromatographic performance of P-CTS-MgO-ZrO2 was systemically evaluated by studying effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the novel SP provided hydrophilic, electrostatic-repulsion and ion-exchange interactions. Compared to the bare MgO-ZrO2, P-CTS-MgO-ZrO2 exhibited superior peak shape, reasonable resolution and reduced analysis time in separation of basic analytes. Besides, remarkable resolving power of acids, i.e. six non-steroidal anti-inflammatory drugs which failed to be eluted from the bare MgO-ZrO2, was obtained with the theoretical plate number (N/m) of 4653-31313, asymmetry factor <1.21 and the resolution of 1.6-3.4. Finally, P-CTS-MgO-ZrO2 SP was applied to separate monosaccharides, phospholipids and peptides. P-CTS-MgO-ZrO2 was a promising hydrophilic SP for wide applications. Copyright © 2014 Elsevier B.V. All rights reserved.
    No preview · Article · Jan 2015 · Analytica Chimica Acta
  • Ting Zhang · Jun-Fang Guo · Li Bai · Zhi-Guo Shi · Li-Ming Qi
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    ABSTRACT: A simple dispersive liquid–liquid microextraction (DLLME) method, based on retrieval of the extraction solvent by magnetic particles and followed by HPLC–UV analysis, was proposed for the determination of UV filters in environmental water samples. The new method uses vortex agitation in the DLLME process to achieve rapid equilibrium. Another feature of the proposed method is that the retrieval of the extraction solvent is accomplished by functionalized magnetic particles rather than by special designed vessels or refrigeration and thawing process associated with DLLME when low-density extraction solvent was used. The parameters that may influence the extraction, including vortex time, volume of extraction solvent, weight of the magnetic particles, volume of desorption solvent, pH, and ionic concentration of the sample solution, were investigated in detail. Under the optimized conditions, the limits of detection for the target UV filters were in range of 0.7–12.3 ng mL−1. The results also demonstrated good linearity and precision, with the regression coefficients above 0.9989 and the RSDs below 4.4%, respectively.
    No preview · Article · Jan 2015 · Journal of Liquid Chromatography & Related Technologies
  • Minqiong Tang · Qing Wang · Ming Jiang · Li Xu · Zhi-Guo Shi · Ting Zhang · Yan Liu
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    ABSTRACT: In the present study, magnetic nanoparticles (MNPs) with phenyl functionalized core and a hydrophilic methylcellulose coating were synthesized. The functionalized MNPs showed excellent dispersibility in aqueous solution and they were applied to magnetic solid phase extraction (MSPE) of sildenafil and its metabolite, desmethyl sildenafil, from human urine and plasma samples followed by high performance liquid chromatographic analysis. The factors that may influence the extraction, including the amount of MNPs, pH and salt concentration of sample solution, extraction and desorption time, and the volume of desorption solvent, were investigated in detail. Under the optimum MSPE conditions, the developed method showed satisfactory reproducibility with intra-day and inter-day relative standard deviations less than 8.2% and low limits of detection of 0.41-0.96ngmL(-1) from urine and plasma samples. The proposed material possessed good water compatibility and demonstrated excellent applicability for biological samples.
    No preview · Article · Dec 2014 · Talanta
  • Qing Wang · Jing Li · Xin Yang · Li Xu · Zhi-Guo Shi · Lan-Ying Xu
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    ABSTRACT: In the current study, zirconia (ZrO2) and its composite, magnesia-zirconia (MgO-ZrO2), were prepared as the hydrophilic interaction chromatographic (HILIC) stationary phases (SPs). Different experimental variables including water content, pH and buffer concentration in the mobile phase (MP) as well as column temperature were systematically studied to permit an in-depth understanding of the chromatographic properties of the mentioned SPs and to explore the retention mechanism further on. The results were compared with a native SiO2 column. Adsorption was demonstrated as the main retention mechanism on the two ZrO2-based SPs. The transferring of the analytes from the MP to the ZrO2-based SPs was endothermic and high column temperature would facilitate the retention. In addition, the MgO-ZrO2 SP exhibited superior resolution, column efficiency as well as stronger retention in comparison to the bare ZrO2 SP, which demonstrated that the introduction of MgO could improve the structure and properties of the material. In conclusion, MgO-ZrO2 was a promising material for HILIC applications.
    No preview · Article · Nov 2014 · Talanta
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    ABSTRACT: Hybrid thiol-silica spheres constructed by interlacing arrays of submicrometer-sized blocks and large macropores were fabricated by a one-pot approach and were successfully used for the fast capture of heavy metal ions with a total capture time of less than 2 min.
    No preview · Article · Aug 2014 · Materials Letters
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    ABSTRACT: In this work, a tandem reversible addition fragmentation chain transfer (RAFT)/click chemistry method was developed to prepare amide-polystyrene-silica (NHCO-Psty-silica) stationary phase. Styrene was immobilized on amino-silica surface via an azide functionalized RAFT agent in a one-pot procedure. The resultant NHCO-Psty-silica column demonstrates better performance for shielding of residue silanols than traditional ODS column, which was ascertained by Engelhardt test (E test), Tanaka test (T test), Galushko test (G test), and Walters test (W test). Our results showed lower values of silanol activity calculated according to the formula in these standard tests for NHCO-Psty-silica column compared to the ODS column we tested. As a result, the NHCO-Psty-silica is suitable for the separation of basic compounds. The hydrophobic, anion exchanging and π-π interaction of the column toward analytes were also evaluated. Moreover, the NHCO-Psty-silica column also showed excellent stability with pure water as mobile phase.
    Full-text · Article · May 2014 · Journal of Chromatography A
  • Long Tao · Lan-Ying Xu · Ju-Ping Wang · Zhi-Guo Shi
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    ABSTRACT: Exposed heavy metal ions have led to serious pollution and significant health problems to human beings. To eliminate these ions from the environment, in this study, a remediation method based on recycling magnetic nanoparticles is proposed. Long alkyl chain derivatized magnetic nanoparticles were fabricated, and then dodecyl mercaptan was assembled onto the functional nanoparticles via hydrophobic interaction. Since mercapto moieties can form molecular complex with many heavy metal ions via chelation, the proposed material was used to retrieve these ions from water samples. It is noteworthy that, as dodecyl mercaptan was attached onto the magnetic particles via hydrophobic interaction, it can be easily removed by washing with methanol. As a result, the nanoparticles can be recovered for recycling usage, which would be economical for environmental remediation. The material was characterized by scanning electron microscopy, nitrogen sorption, infrared/Raman spectrometry and elemental analysis. Ag+and Pb2+were used as model analytes to evaluate the effectiveness of the material. It is found that, the maximum adsorption capacities of the particles for Ag+and Pb2+reach 43.6 and 38.2mg/g, respectively.
    No preview · Article · Dec 2013 · Materials and Manufacturing Processes
  • Chen-Ru Yin · Li-Yun Ma · Jian-Geng Huang · Li Xu · Zhi-Guo Shi
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    ABSTRACT: Penetrable silica possesses hierarchical pores, mesopores and penetrable macropores, offering fast mass transfer, satisfactory mechanical strength as well as low column pressure. In the present study, penetrable octadecyl-bonded silica (ODS) was for the first time used as biopartitioning micellar chromatography (BMC) stationary phase to profile ecotoxicity and skin permeability of benzophenone UV-filters. Mobile phase (MP) pH and concentration of polyoxyethylene(23)lauryl ether in the MP were systematically studied. Quantitative retention-activity relationships (QRARs) model was established to correlate retention factors (k) on BMC with bioconcentration factor (BCF) and transdermal rate (TR) of UV-filters. Coefficient of determination (r(2)) of the QRARs model between log BCF and logk were 0.9398-0.9753, while r(2) between TR and logk were 0.7569-0.8434, which demonstrated satisfactory predictive ability of the methodology. It was a powerful tool for fast screening by combining penetrable ODS with BMC, and avoiding column blockage often occurring in BMC.
    No preview · Article · Dec 2013 · Analytica chimica acta
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    ABSTRACT: Cytokinins (CKs), a vital family of phytohormones, play important roles in the regulation of shoot and root development. However, the quantification of CKs in plant samples is frequently affected by the complex plant matrix. In the current study, we developed a simple, rapid and efficient hydrophilic interaction chromatography-solid phase extraction (HILIC-SPE) method for CKs purification. CKs were extracted by acetonitrile (ACN) followed by HILIC-SPE (silica as sorbents) purification. The extraction solution of plant samples could be directly applied to HILIC-SPE without solvent evaporation step, which simplified the analysis process. Moreover, with HILIC chromatographic retention mechanism, the hydrophobic co-extracted impurities were efficiently removed. Subsequently, CKs were separated by RPLC, orthogonal to the HILIC pretreatment process, and detected by tandem mass spectrometry. The method exhibits high specificity and recovery yield (>77.0%). Good linearities were obtained for all eight CKs ranging from 0.002 to 100ngmL(-1) with correlation coefficients (r) higher than 0.9927. The limits of detection (LODs, signal/noise=5) for the CKs were between 1.0 and 12.4pgmL(-1). Reproducibility of the method was evaluated by intra-day and inter-day measurements and the results showed that relative standard deviations (RSDs) were less than 10.5%. Employing this method, we successfully quantified six CKs in 20mg Oryza sativa leaves and the method was also successfully applied to Brassica napus (flower and leaves).
    Full-text · Article · Oct 2013 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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    ABSTRACT: SiO2-TiO2 composite fibers, prepared by electrospinning, were successfully applied to the rapid enrichment of phosphopeptides using a lab-in-syringe approach for the first time. Because of their large surface area, mesoporous structure, extraordinary length and appropriate Lewis acidity, the as-prepared SiO2-TiO2 composite fibers exhibited high selectivity and capacity in the enrichment of phosphopeptides from the digestion mixture of β-casein and bovine serum albumin (BSA), as well as human blood serum and nonfat milk. The targeted phosphopeptides could be easily enriched and detected even when the total amount of β-casein was decreased to only 10 fmol, indicating the high detection sensitivity of this method. In addition, the whole enrichment extraction procedure can be finished in less than 3 min, which can avoid or decrease the degradation of endogenous phosphoproteins by proteases released ex vivo during time-consuming treatments. The developed method is rapid, cost-effective, selective, sensitive, operationally simple, and does not require any harsh conditions and intricate equipment, providing an ideal candidate for the enrichment of phosphopeptides from complex biological samples either in the lab or in the field.
    Full-text · Article · Jul 2013 · The Analyst