Publications (88)

  • Yaqing Chang · Zhe Zhang · Huiqing Liu · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Detection of specific DNA sequences is critical in life science. In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe. ssDNA could adsorb on the CoOOH surface via electrostatic interactions and therefore the fluorescence of FAM was quenched. However, upon addition of targets, ssDNA was hybridized with target DNA and the formed double-stranded DNA (dsDNA) had much weaker affinity to CoOOH, resulting in the retaining of fluorescence. Based on the affinity difference of CoOOH nanoflakes to ssDNA and dsDNA and fluorescence resonance energy transfer based fluorescence quenching, a mix-and-detect method was proposed for homogeneous detection of DNA. The proposed method is simple and can be finished in a few minutes with high sensitivity. Furthermore, it displays a wide linear range from 1 to 50 nM with a detection limit of 0.5 nM and is capable of detecting DNA in real biological samples.
    Article · Jun 2016 · The Analyst
  • Huiqing Liu · Haiyan Qiao · Daniela Krajcikova · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: The spore of Bacillus subtilis, a dormant type of cell, is surrounded by a complex multilayered protein structure known as the coat. It is composed of over 70 proteins and essential for the spore to withstand extreme environmental conditions and allow germination under favorable conditions. However, understanding how the properties of the coat arise from the interactions among all these proteins is an important challenge. Moreover, many specific protein–protein interactions among the coat proteins are crucial for coat assembly. In this study, atomic force microscopy (AFM) based single molecule force spectroscopy (SMFS) was applied to investigate the interaction as a dynamic process between two morphogenetic coat proteins, CotE and CotZ. The unbinding force and kinetic parameters characterizing the interaction between CotE and CotZ were obtained. It is found that there is a strong affinity between CotE and CotZ. Furthermore, the assembly behaviors of CotE and CotZ, individually or in combination, were studied by AFM at solid-liquid interfaces. Our results revealed that CotE–CotZ assembly is dependent on their molar ratios and the interaction between CotE and CotZ involves in the CotE–CotZ assembly.
    Article · Jun 2016 · Journal of Structural Biology
  • Yaqing Chang · Zhe Zhang · Jinhui Hao · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Rapid and sensitive detection of Ag+ in aquatic ecosystems is of great significance for public health and environmental security. In this report, a simple and feasible colorimetric method for the detection of Ag+ at an ultralow concentration is developed. Bovine serum albumin stabilized gold nanoclusters ([email protected]/* */) possess the peroxidase-like activity that could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2. Once Ag+ is introduced, Ag+ selectively reacts with Au° through redox reaction, which induces an apparent inhibition of the peroxidase-like activity of [email protected]/* */ On the basis of this effect, a colorimetric Ag+ sensor is established. The high specificity of the Ag-Au interaction provides excellent selectivity over the interfering metal ions. The lowest detectable concentration for Ag+ is 0.204 μM. And this method could be applied for the rapid analysis of Ag+ in lake water with satisfactory results. These advantages demonstrate that the proposed method is a promising candidate for the detection of Ag+ in water samples.
    Article · Apr 2016 · Sensors and Actuators B Chemical
  • Huiqing Liu · Daniela Krajcikova · Nan Wang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Spores are uniquely stable cell types that are produced when bacteria encounter nutrient limitations. Spores are encased in a complex multilayered coat, which provides protection against environmental insults. The spore coat of Bacillus subtilis is composed of around 70 individual proteins that are organized into four distinct layers. Here we explored how morphogenetic protein CotE guides formation of the outermost layer of the coat, the crust, around the forespore by focusing on three proteins: CotE, CotY and CotX. Single molecule force spectroscopy (SMFS) was used to investigate the interactions among CotE, CotY, and CotX at the single-molecule level. Direct interactions among these three proteins were observed. Additionally, the dissociation kinetics was also studied by measuring the unbinding forces of the complexes at different loading rates. A series of kinetic data of these complexes were acquired. It was found that the interaction of CotE and CotY was stronger than that of CotE and CotX.
    Article · Jan 2016 · The Journal of Physical Chemistry B
  • Yaqing Chang · Zhe Zhang · Jinhui Hao · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: The detection of glyphosate (Glyp) in water is of increasing importance because of its potential danger to environmental and health. In this study, a simple and label free colorimetric method for Glyp detection is proposed based on the inhibition of peroxidase-like activity of Cu2+. Cu2+ possesses the peroxidase-like activity that could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) in the presence of H2O2, resulting in a color change of the solution. However, the peroxidase-like activity of Cu2+ could be strongly hindered by Glyp due to the formation of Glyp-Cu2+ complexes. The color change of the solution or the absorbance change of oxTMB is utilized to monitor the concentration of Glyp. The linear range of the proposed method is 2 to 200 μM and the detection limit is 1 μM. Furthermore, as low as 10 μM Glyp can be clearly distinguished by naked eyes. Also this proposed method is successfully applied for screening Glyp in real samples and shows great promise for environmental monitoring owing to its simplicity, rapidness and high selectivity.
    Article · Jan 2016 · Sensors and Actuators B Chemical
  • Zhe Zhang · Jinhui Hao · Wenshu Yang · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: The development of an efficient and affordable bifunctional electrode for full water splitting in basic and neutral conditions is still a challenging issue in obtaining clean and sustainable chemical fuels. Herein, a unique bifunctional electrode consisting of 3D trinary transition-metal phosphide nanosheets with abundant reactive sites anchored on Ni foam, referred to as FexCoyNizP, has been developed through a facile two-step process of electrodeposition and low-temperature phosphidation. When used as a bifunctional electrode for the full water splitting, an applied voltage of 1.57 V is required for a current density of 10 mA cm-2.
    Article · Jan 2016 · RSC Advances
  • Huiqing Liu · Daniela Krajcikova · Zhe Zhang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Spores formed by Bacillus subtilis are surrounded by a protective and multilayered shell, termed the coat, which grants the spores resistance to various environmental stresses and facilitates spore germination. The spore coat consists of more than seventy different proteins, arranged into at least four distinct structural layers: the undercoat, inner coat, outer coat and crust. However, how these proteins, especially the morphogenetic proteins, interact to establish the organized, functional coat layers remains poorly understood. CotY and CotZ as the components of the crust, play a morphogenetic role in the crust assembly around the spore. In this study, the single molecule force spectroscopy was used to investigate the interaction an{Qiao, 2013 #44}d dynamics between CotY and CotZ at the single-molecule level. The results show that homotypic interactions of CotY and CotZ and the heterotypic interaction between CotY and CotZ exist. Furthermore, the dissociation kinetics of the complexes were studied by monitoring the relationship between the unbinding forces and the loading rates at different pulling velocities. In this way, a series of kinetic parameters regarding the three different complexes were obtained. It revealed the strong interactions between CotY and CotZ, CotY and CotY, and a relatively weak interaction of CotZ and CotZ. Copyright © 2015. Published by Elsevier Inc.
    Article · Sep 2015 · Journal of Structural Biology
  • Nan Wang · Huiqing Liu · Jinhui Hao · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Epithelial cell adhesion molecule (EpCAM) is a tumor-specific antigen for malignancies of epithelialis lineage. In this study the interaction of DNA-based EpCAM aptamer (SYL3C) and EpCAM was explored with single molecular recognition force spectroscopy (SMFS). The capability of aptamer SYL3C to recognize EpCAM protein and the kinetic parameters were investigated.
    Article · Jul 2015 · The Analyst
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    [Show abstract] [Hide abstract] ABSTRACT: Supporting Information
    Full-text Dataset · Jul 2015
  • Zhe Zhang · Jinhui Hao · Wenshu Yang · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: A defect-rich CoP/nitrogen-doped carbon composite is reported for the first time derived from ZIF-67 by means of low-temperature phosphidation process. As a hydrogen evolution reaction electrocatalyst, the obtained CoP-N-C has high HER activity and good stability with a low onset overpotential of 31mV, a small Tafel slope of 42mVdec-1, a large exchange current density of 1.6×10-1mAcm-2, and a 10mAcm-2 current density at overpotential 91mV. It's all for HER: A defect-rich CoP/nitrogen-doped carbon composite is synthesized for the first time from metal- organic frameworks. As a hydrogen evolution reaction electrocatalyst, this composite shows high activity and good stability with a small Tafel slope of 42mVdec-1, a low onset overpotential of 31mV, and a large exchange current density of 1.6×10-1mAcm-2.
    Article · Jun 2015 · ChemCatChem
  • Huiyan Li · Xiaojing Bai · Nan Wang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: We present an aptamer-based microcantilever biosensor for label-free detection of nucleolin. The sensor cantilevers in the microcantilever array were functionalized with nucleolin aptamer (AS1411) while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate environmental disturbances. The interaction between nucleolin and AS1411 induced surface stress changes, resulting in a differential deflection between sensor and reference cantilevers. The amplitude of differential cantilever deflection had a good linear relationship with the nucleolin concentration ranging from 10 nM to 250 nM with a correlation coefficient of 0.999. The detection limit was about 1.0 nM, at a signal-to-noise ratio of 3. The aptamer-based microcantilever sensor demonstrated good selectivity and was facile, rapid, and reagentless. Our results show the potential for the application of microcantilever biosensor system as a powerful tool to detect tumor markers with high sensitivity and specificity.
    Article · Jun 2015 · Talanta
  • Jinhui Hao · Wenshu Yang · Zhe Zhang · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Designing and developing active, cost-effective and stable electrocatalysts for hydrogen evolution reaction (HER) are still ongoing challenge. Herein, we report the synthesis of binary transition metal phosphides (CoxFe1-xP) nanocubes with different Co and Fe ratio through a phosphidation process by using metal−organic frameworks (MOFs) as templates. MOFs templates contribute well-defined nanocubes architecture features after phosphidation, while suitable phosphidation temperature could construct crystal structure and keep the well-defined structure. The incorporation of binary transition metal results in redistributions of the valence electrons in CoxFe1-xP. The changes imply anionic states of the P and Fe atoms, which act as active sites and thus stronger electron-donating ability. When CoxFe1-xP nanocubes are employed as electrocatalysts, these characteristic features facilitate the performance of HER. Remarkably, Co0.59Fe0.41P nanocubes prepared at 450 oC afford a current density of 10 mA cm-2 at a low overpotential of 72 mV in acidic condition and 92 mV in alkaline condition. Co0.59Fe0.41P nanocubes also exhibit a small Tafel slope of 52 mV decade−1 in acidic condition and 72 mV decade−1 in alkaline condition. Moreover, Co0.59Fe0.41P nanocubes show good stability in both acidic and alkaline conditions. Our method produces the highly active HER catalyst based on binary transition metal MOFs templates, providing a new avenue for designing excellent electrocatalysts.
    Article · May 2015 · Nanoscale
  • Jinhui Hao · Wenshu Yang · Zhe Zhang · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Three-dimensional Prussian blue-reduced graphene oxide hydrogel was synthesized with the assistant of sodium dodecyl sulfate (SDS) through a facile hydrothermal method. The hydrogel exhibited strong mechanical properties and was successfully applied as a self-propulsion motor for water treatment. During the self-propulsion degradation process, SDS helped oxygen bubbles quickly exhausting from motor and the oxygen bubbles assisted the rapid diffusion of hydroxyl radicals. In addition, the well-defined structure increased the number of reaction sites and the synergy between reduced graphene oxide and Prussian blue accelerated degradation efficiency. The self-propulsion motor had the average velocity of 0.026±0.013 cm s-1 in 7.5% H2O2 and 0.069±0.032 cm s-1 in 22.5% H2O2. Moreover, the self-propulsion motor keeps high degradation efficiency even after cycling for 9 times. These excellent properties make the motor an ideal candidate for self-propulsion water treatment.
    Article · May 2015 · Nanoscale
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    [Show abstract] [Hide abstract] ABSTRACT: Bacterial spores (endospores), such as those of the pathogens Clostridium difficile and Bacillus anthracis, are uniquely stable cell forms, highly resistant to harsh environmental insults. Bacillus subtilis is the best studied spore-former and we have used it to address the question of how the spore coat is assembled from multiple components to form a robust, protective superstructure. B. subtilis coat proteins (CotY, CotE, CotV and CotW) expressed in E. coli can arrange intracellularly into highly stable macro-structures through processes of self-assembly. Using electron microscopy we demonstrate the capacity of these proteins to generate ordered one dimensional fibres, two dimensional sheets and three dimensional stacks. In one case (CotY), the high degree of order favours strong, co-operative intracellular disulfide cross-linking. Assemblies of this kind could form exquisitely adapted building blocks for higher order assembly across all spore-formers. These physically robust arrayed units could also have novel applications in nano-biotechnology processes. This article is protected by copyright. All rights reserved.
    Full-text Article · Apr 2015 · Molecular Microbiology
  • Zhe Zhang · Jinhui Hao · Wenshu Yang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Developing inexpensive and highly efficient non-precious-metal electrocatalysts has been proposed as a promising alternative to platinum-based catalysts for the hydrogen evolution reaction (HER). Herein, we report novel FeP NPs supported on inexpensive and available candle soot (FeP-CS) derived from Fe3O4-CS hybrid precursors obtained after a phosphidation reaction. As HER electrocatalysts, the FeP-CS hybrids exhibit high electrocatalytic ability for HER with a Tafel slope of 58 mV dec(-1), a low onset overpotential of 38 mV, a large exchange current density of 2.2 × 10(-1) mA cm(-2) and an overpotential of 112 mV to obtain a current of 10 mA cm(-2). The present work shows significant advance in designing and developing non-precious-metal electrocatalysts for hydrogen evolution reaction.
    Article · Feb 2015 · Nanoscale
  • Jinhui Hao · Wenshu Yang · Zhe Zhang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: A facile hydrothermal method combined with a post solution reaction is developed to synthesize interconnected three-dimension (3D) hierarchical Co3-xFexO4 ferrite (CF) on nickel foam. By controlling the experimental parameters, the structures of the products are tailored from nanoflowers to microflowers with different sized void interiors. The obtained 3D hierarchical flower-like CF are characterized by field emission scanning electron microscopy, X-ray diffraction, and inductively coupled plasma mass spectrometer analysis. The 3D hierarchical flower-like CF-nickel foam with the rational structural feature could be used as binder and conductive agent-free supercapacitor electrodes directly. Such integrated electrodes exhibit a high specific capacitance and well cycling stability when the charge-discharge current density is high. Remarkably, the 3D hierarchical CF microflowers exhibit specific capacitance values of 768 F g−1 at a constant current density of 6 A g−1. The CF microflowers also show high charge-discharge reversibility with an efficiency of 79.2% after 5000 cycles.
    Article · Nov 2014 · Electrochimica Acta
  • Azhar Rasul · Xiaoyan Shen · Bingyu Wang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Tubeimoside-1 (TBMS1) is a triterpenoid saponin with potent anticancer properties. In this study, for the first, we examined the anti-proliferative effects of TBMS1 in human bladder cancer T24 cells and its ability to induce apoptosis and cell cycle arrest. Our results demonstrated that TBMS1 decreased the cell viability of bladder cancer T24 cells in a dose-dependent manner. Flow cytometric analysis showed that TBMS1 significantly triggered apoptosis in T24 cells and arrested cell cycle at G2/M phase in a dose-dependent manner. Further characterization demonstrated that TBMS1-induced apoptosis is associated with dissipation in mitochondrial membrane potential (ΔΨm), down-regulation of Bcl-2, and up-regulation of Bax and p21 in TBMS1-treated T24 cells. These in vitro results suggested that TBMS1 is an effective anti-bladder cancer natural compound that worth further mechanistic and therapeutic studies in human bladder cancer. © 2014 Bangladesh Pharmacological Society. All Rights reserved.
    Article · Sep 2014 · Bangladesh Journal of Pharmacology
  • Zhe Zhang · Baoping Lu · Jinhui Hao · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: A synthetic route to FeP-GS hybrid sheets that show good stability and high electrocatalytic activity for hydrogen evolution reaction is reported. The materials are prepared via thermal phosphidation of pre-synthesized Fe3O4-GS hybrid sheets.
    Article · Aug 2014 · Chemical Communications
  • Wenshu Yang · Jinhui Hao · Zhe Zhang · [...] · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: A facile approach was proposed for the synthesis of hierarchical CoxFe3−xO4 nanocubes (CF nanocubes), using Prussian Blue (PB) as precursor. The method consists of the synthesis of hierarchical cobalt–iron Prussian blue analogues (PBAs) through the reaction of PB nanocubes with CoCl2 in a water bath and subsequently calcining the corresponding PBA precursor. The obtained CF nanocubes were characterized using transmission electron microscopy, scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and N2 adsorption–desorption isotherm measurements. It is found that the hierarchical CF nanocubes have a large specific surface area (108 m2 g−1). Considering the hierarchical structure and the doping of Co in Fe3O4 are beneficial for the catalytic activity of the catalyst, an efficient and simple colorimetric biosensor for H2O2 and glucose was fabricated using CF nanocubes as a peroxidase mimetic. The good catalytic activity and low-cost make the hierarchical CF nanocubes a useful biocatalyst for a wide range of potential applications in medicine and biotechnology.
    Article · Aug 2014 · RSC Advances
  • Baoping Lu · Jilin Tang
    [Show abstract] [Hide abstract] ABSTRACT: Ultrathin two-dimensional (2D) nanosheets of layered transition-metal chalcogenide are theoretically and technologically intriguing. However, it still remains a great challenge to synthesize ultrathin nanosheets because of the lack of an intrinsic driving force for anisotropic growth of 2D superposed microstructures. Here we demonstrate, for the first time to our knowledge, the in situ synthesis of large-scale ultrathin Sb2S3 nanosheets on graphene sheets (G) by solvothermal method in the water/ethylene glycol mixed solvent. Owing to the synergetic chemical coupling effects between G and Sb2S3, Sb2S3–G hybrid nanosheets exhibit high catalytic performance for degradation of methylene blue in the presence of H2O2. In the meantime, it was found that the resulting Sb2S3–G displays good electrocatalytic activity towards the hydrazine oxidation. This work not only offers a low-cost and high performance alternative technology for synthesizing sheet-like Sb2S3, but also opens the door toward fabrication of varying types of metal sulfide–graphene nanomaterials that will have wide application in the catalysis, environmental, and new energy fields.
    Article · Jul 2014 · Dalton Transactions