Yoshihisa Takahata

Hiroshima University, Hirosima, Hiroshima, Japan

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Publications (41)52.49 Total impact

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    ABSTRACT: We investigated the postprandial thermic effect of chicken and its mechanisms in rats. A chicken diet showed a strong thermic effect after consumption, and the removal of fat induced more rapid and stronger thermogenesis. Although thermogenesis induced by a purified chicken protein diet was also strong, the thermic reaction was not so rapid and a remarkable rise of peripheral temperatures was not observed. Defatted chicken and purified chicken protein activated the thyroid hormone system and up-regulated rate-limiting enzyme genes of glucose metabolism and the tricarboxylic acid (TCA) cycle in the liver. Moreover, chicken protein up-regulated the mRNA expression of a rate-limiting enzyme of hepatic lipid metabolism. It is possible that the mechanisms by which body temperature is raised are different between chicken protein and defatted chicken. On the other hand, it is possible that chicken fat suppressed the expression of energy metabolism-related genes that was induced by the consumption of lean chicken. As a result, a rise of postprandial body temperature might not have been induced after consumption of chicken fat. These results suggest that the consumption of lean chicken activates the thyroid hormone system and hepatic energy metabolism and consequently induces the postprandial thermic effect of chicken.
    No preview · Article · Dec 2013 · Journal of Nutritional Science and Vitaminology
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    ABSTRACT: We investigated the effects of a chicken collagen hydrolysate (CCH) on the circulation system in humans. A total of 58 subjects with either mild hypertension (systolic blood pressure (SBP) of 140-159 mm Hg or diastolic blood pressure (DBP) 90-99 mm Hg) or high-normal blood pressure (SBP 130-139 mm Hg or DBP 85-89 mm Hg) were assigned to two groups, one involving a placebo and the other, the test food (including CCH of 2.9 g/d). The parameters related to each subject's circulation system were monitored over the study period of 18 weeks. The Δbrachial-ankle pulse wave velocity (baPWV), an indicator of arterial stiffness and marker of vascular damage, was significantly lower in the test food group than in the placebo group during the treatment period. The blood pressure in the test food group was also significantly lower than that in the placebo group, while the serum nitrogen oxide (NOx) was higher in the test food group after the treatment. These results suggest that CCH exerted modulatory effects on the human circulation system.
    No preview · Article · Apr 2013 · Bioscience Biotechnology and Biochemistry
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    ABSTRACT: In this study, we investigated the anti-hypertensive effects of two enzymatic hydrolysates of chicken and pig collagens in spontaneously hypertensive rats (SHR). SHR were divided into three groups and administrated collagen peptides at 3 g/kg body weight, or water for 14 weeks. In the chicken collagen peptides group, systolic blood pressure was significantly reduced when compared with the control group, and diastolic blood pressure was also reduced when compared to the pig collagen group. Microscopic analysis revealed that inflammation in liver and heart was mildly ameliorated in the chicken collagen peptides group.
    No preview · Article · Jan 2013 · Nippon Shokuhin Kagaku Kogaku Kaishi
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    ABSTRACT: Several studies have shown a relationship between depression and animal protein intake. To evaluate whether the difference of dietary chicken protein levels induces an antidepressant-like effect and potentiates acute antidepressant effects, three levels of dietary chicken protein were used as the representative animal protein with imipramine used as the antidepressant. In addition, the effects of dietary chicken protein on brain metabolism were evaluated. Open field test (OFT) and forced swimming test (FST) were conducted on the 27th and 28th days, respectively. OFT and FST were not influenced by both imipramine and dietary protein levels. However, characteristic effects of imipramine treatment on brain monoamine metabolism were observed in the cerebral cortex and hypothalamus. In addition, dietary protein significantly increased taurine and L-ornithine levels even though these amino acids were not contained in the diets. In conclusion, the metabolism of several amino acids in the plasma and brain were altered by dietary chicken protein.
    No preview · Article · Dec 2012 · Animal Science Journal
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    ABSTRACT: Several amino acids have effects on mental function, including sedative, antidepressant-like and anxiolytic-like effects. However, the influence of integrated amino acid nutrition as protein constituents on mental function remains unclear. Therefore, the purpose of the present study was to compare the influence of chicken, pork and beef protein extracts on brain monoamine metabolism in mice. Changes in monoamine levels and their turnover rates in the brain were induced by different protein sources. In particular, chicken protein group showed the highest norepinephrine levels in the hippocampus and hypothalamus, and beef protein extract caused an activation of the serotonergic system in the hypothalamus, although there were no significant differences in amino acid compositions of these protein extracts. Therefore, it was revealed that amino acid compositions in dietary protein did not induce alteration in monoamine metabolism. However, there were differences in small molecular peptides, such as creatine, carnosine and anserine levels in animal protein extracts. In conclusion, monoamine metabolism was altered by dietary protein sources. However, it was indicated that the alteration in monoamine metabolism may be independent from amino acid compositions in dietary protein. In addition, alteration in monoamine metabolism depending on the dietary protein sources may be induced by small molecular peptides.
    No preview · Article · Jun 2012 · Animal Science Journal
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    ABSTRACT: Carnosine is a dipeptide that improves exercise performance. The carnosine synthesis mechanism through carnosine and ß-alanine ingestion remains unclear. Therefore, we investigated the tissue distribution of carnosine synthase, ATP-grasp domain-containing protein-1 (ATPGD1) mRNA, and ATPGD1 and carnosine specific dipeptidase (CN1) gene expression profiles in mice that were given carnosine or ß-alanine orally. ddY mice (7-week-old) were randomly divided into three groups (n = 6 to 8 animals per group) and were orally given 2 g/kg body weight of carnosine, ß-alanine, or water. After 15, 30, 60, 120, 180, or 360 min of treatment, the tissues (brain, blood, liver, kidneys, olfactory bulbs, hindleg muscles) were collected. The obtained tissues measured the expression of ATPGD1 and CN1 genes using quantitative PCR methods. The ATPGD1 gene was expressed in muscle and to a lesser extent in brain. The expression of ATPGD1 in the vastus lateralis muscle increased significantly at 180 min (P = 0.023) after carnosine ingestion and 60 (P = 0.023) and 180 min (P = 0.025) after ß-alanine ingestion. Moreover, the carnosine group showed a significantly increased renal expression of the CN1 gene 60 min after ingestion (P = 0.0015). The ATPGD1 gene showed high expression levels in brain and muscle. The ß-alanine or carnosine administration significantly increased ATPGD1 and CN1 expression in mice.
    Preview · Article · Apr 2012 · Journal of the International Society of Sports Nutrition
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    Tomomi Kouguchi · Youzuo Zhang · Mikako Sato · Yoshihisa Takahata · Fumiki Morimatsu

    Preview · Chapter · Jan 2012
  • Mikako Sato · Hirohiko Maemura · Yoshihisa Takahata · Fumiki Morimatsu · Yuji Sato
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    ABSTRACT: The effects of carnosine and anserine rich chicken extract on muscular power and fatigue sensation in middle and advanced age groups were investigated. Twenty middle and advanced aged healthy volunteers were divided into two groups and administered chicken extract at 1500 mg/day (225 mg/day as carnosine + anserine) or 0 mg/day for 4 weeks. Before and after the administration period, isokinetic knee extension power, flexion power, and one-leg standing time with eyes open were assessed. Chicken extract administration significantly increased knee extension power and one-leg standing time. These results suggest that carnosine- and anserine-rich chicken extract administration effectively improves muscular power in middle and advanced age.
    No preview · Article · Jan 2012 · Nippon Shokuhin Kagaku Kogaku Kaishi
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    ABSTRACT: Novel peptides derived from chicken collagen hydrolysate (CCH) were confirmed to have a vasoprotective effect after ingestion and absorption to vessels; however, their endovascular mechanism of action required clarification. Therefore, using human umbilical vein endothelial cells (HUVECs), in which inflammation had been induced by tumor necrosis factor-α (TNF-α), we investigated the mechanism by which CCH-derived peptides inhibited the vascular inflammatory response. Expression of soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), and interleukin-8 (IL-8) in HUVECs was increased significantly by the addition of TNF-α, whereas treatment with proline-hydroxyproline (Pro-Hyp) and hydroxyproline-glycine (Hyp-Gly), which are present in human peripheral blood after ingestion of CCH, inhibited these increases. These peptides also reduced the mRNA expression of inducible nitric oxide synthase (iNOS) up-regulated by TNF-α. These results suggest that the vasoprotective effect of CCH-derived peptides is due to inhibition of endothelial inflammation.
    No preview · Article · Jan 2012 · Food Science and Technology Research
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    ABSTRACT: The effect of oral administration of elastin peptide (hydrolysate of elastin from porcine aorta) on human skin elasticity was studied. First, five healthy volunteers were administered elastin peptide after fasting for 12 h. A significant increase in total amino acid concentration in blood was observed. The composition of increased amino acids in blood was similar to the amino acid composition of the elastin peptide. In addition, the concentrations of peptide forms of hydroxyproline and arginine significantly increased in blood. These results suggest that a portion of the administered elastin peptide migrates into blood in peptide form. Second, thirty-nine middle-aged volunteers were divided into three groups and administered 0, 100, or 200 mg/day of elastin peptide for 8 weeks. Significant increases in skin elasticity were observed in the 100- and 200-mg groups after 8 weeks. Increase in skin elasticity of the 200-mg group was significantly higher than that in the 0-mg group. These results suggest that ingestion of elastin peptide increases skin elasticity.
    No preview · Article · Jan 2011 · Nippon Shokuhin Kagaku Kogaku Kaishi
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    ABSTRACT: Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro (GAXGLXGP, X: Hyp), an octapeptide contained in chicken collagen hydrolysate, inhibits angiotensin I-converting enzyme activity in vitro. Intestinal Caco-2 and bovine aortic endothelial cells (BAECs) were used to investigate whether the transported GAXGLXGP improves vascular function. When GAXGLXGP was added to the apical side of Caco-2 monolayers, the intact form of GAXGLXGP was released to the basolateral side without incorporation into the cells. This transport was energy-independent but was associated with tight junction permeability. GAXGLXGP was then added to BAECs, and endothelial nitric oxide (NO) synthase (eNOS) activation was examined. GAXGLXGP at a concentration of 10 microM stimulated production of NO during a 1 h incubation. This event involved phosphorylation of eNOS at Ser(1179) without a change in the total eNOS protein level. These findings indicate that GAXGLXGP absorbed intact through the intestinal epithelium has direct effects on eNOS activity in vascular endothelial cells, leading to NO synthesis, thereby suggesting the potential for improvement in vascular function.
    No preview · Article · Jun 2010 · Journal of Agricultural and Food Chemistry
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    ABSTRACT: Effects of chicken collagen hydrolysate (CCH) on atherogenesis in apolipoprotein E-deficient C57BL/6.KOR-ApoE(shl) mice were investigated in this paper. The mice were fed on either a normal CE-2 diet (control group) or a diet containing 10% CCH (CCH group) for 12 wk. Compared with that of the control, the amount of total plasma cholesterol, total hepatic cholesterol and hepatic triglycerides in the CCH group was reduced by 14.4, 24.7 and 42.8%, respectively. Histological analysis results showed that the abundance of diffuse hepatic lipid droplets and fat vacuoles was decreased in the CCH group. Meanwhile, the concentration of proinflammatory cytokines in the CCH group plasma, including interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1), and tumor necrosis factor-alpha (TNF-alpha), was downregulated by 43.4, 17.9 and 24.1%, respectively. The present results suggested that CCH treatment might help prevent atherosclerosis through not only its lipid-lowering effects but also inhibiting expression of inflammatory cytokines.
    No preview · Article · Jun 2010 · Journal of Nutritional Science and Vitaminology
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    ABSTRACT: Intestinal permeability to size-classified fish collagen peptides (FCPs) was examined using Caco-2 monolayers. Minimum-sized FCPs were most efficiently transported across the Caco-2 monolayers. Permeability depended on peptide size. It was independent of the H(+)-coupled peptide transport system but was associated with tight junction permeability, suggesting that the paracellular pathway is responsible for transepithelial transport of collagen peptides.
    No preview · Article · May 2010 · Bioscience Biotechnology and Biochemistry
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    ABSTRACT: We previously reported that chicken collagen hydrolysate (CCH) has strong angiotensin I converting enzyme (ACE) inhibitory activity and antihypertensive effects on spontaneously hypertensive rats. Here, we investigated the chronic therapy effects of CCH on blood pressure and vascular relaxation in a cardiovascular damage model of Wistar-Kyoto rats induced by N-nitro-l-arginine methyl ester (L-NAME). Following co-treatment with CCH for 4 weeks, the increment of systolic blood pressure was suppressed significantly. At 8 weeks, the vasorelaxation of thoracic aorta increased significantly, and cardiovascular damage was ameliorated. The concentration of soluble intercellular adhesion molecule-1 (ICAM-1) in blood was reduced significantly by long-term administration of CCH, whereas the nitric oxide concentration was increased significantly at 1 hour post-treatment. The results suggest that beneficial effects of CCH result from antihypertensive function, but also from inhibition of cardiovascular damage to the endothelial cells via its ACE inhibitory activity and regulation of nitric oxide and ICAM-1, which suggests that CCH may be useful as a medicinal food for patients with cardiovascular disease.
    No preview · Article · Feb 2010 · Journal of medicinal food
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    ABSTRACT: The effect of porcine artery elastin on serum cholesterol level was investigated in rats fed a cholesterol-free diet. Rats were fed for 4 weeks, with a diet (ED) containing 15% casein and 5% of porcine artery elastin in comparison with a diet (CD) containing 20% casein. The total serum and non-HDL-cholesterol concentrations were lower (P < 0.001) in ED-fed group than the CD-fed group at the end of the experiment. Caecal propionate concentration and Bifidobacterium and Lactobacillus population were higher (P < 0.05) in ED-fed group than the CD-fed group. The results of this study suggest that porcine artery elastin could be considered as a functional dietary protein with hypocholesterolaemic ability. Favourable amino acid composition and lysine derived cross links may at least be partially responsible for the hypocholesterolaemic ability of ED. Moreover, the higher caecal propionic acid concentration in the ED-fed group may have suppressed the cholesterol synthesis in the liver, and reduced the serum cholesterol level.
    Full-text · Article · Oct 2009 · Journal of Functional Foods
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    ABSTRACT: Elastic fibers in the dermis play an important role in the elasticity of the skin. The desmosine crosslinking structures produced by lysyl oxidase (LOX) in the elastic fibers are partly responsible for the elasticity. The degradation of elastic fibers by matrix metalloproteinases (MMPs), MMP-12, also plays an important role in the maintenance of elasticity. In the present study, we investigated whether the gene expression in skin for these enzymes can be modified by ingestion of elastin peptide. Mice were given elastin peptides orally by single administration. The mRNA expression of tropoelastin, LOX and MMP-12 in mice skin was then quantified by the real-time reverse transcriptase-polymerase chain reaction. Ingestion of elastin peptides caused an increase in tropoelastin and LOX, but not in MMP-12, mRNA expression. In conclusion, elastin peptides may be effective in prompting the synthesis of elastic fibers in mice.
    No preview · Article · Aug 2009 · Current Topics in Nutraceutical Research
  • Ai Saiga-Egusa · Koji Iwai · Toru Hayakawa · Yoshihisa Takahata · Fumiki Morimatsu
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    ABSTRACT: Chicken collagen hydrolysate was given to 15 mildly hypertensive subjects for 4 weeks. Blood pressure was significantly decreased by 11.8 mmHg (P<0.01). A reduction in plasma renin activity was observed in blood test after intake. A colony assay of endothelial progenitor cells in blood samples from non-smokers revealed an approximately 30% increase in the number of colonies.
    No preview · Article · Feb 2009 · Bioscience Biotechnology and Biochemistry
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    ABSTRACT: In this study, we investigated the concentration of food derived-peptides in blood following oral intake of chicken collagen hydrolysate (chicken-collagen octa peptides ; C-COP) and measured the angiotensin-converting enzyme (ACE) inhibitory activity of these peptides. Healthy volunteers ingested the C-COP solution (25g/60kg body weight) after fasting for 12 h. The peptide-formed hydroxyproline (Hyp) significantly increased and reached maximum levels (91.1 μ M) 2 h after consumption. Pro-Hyp, Leu-Hyp, Pro-Hyp-Gly, Phe-Gly, Ala-Hyp, Hyp-Gly, Glu-Hyp-Gly, Ala-Hyp-Gly and Ser-Hyp-Gly were detected in human blood, possessing ACE-inhibitory activity. Of them, Ala-Hyp (IC50: 0.177 mM) had the strongest ACE inhibitory activity. Thus, C-COP appears to absorb with the peptides by enzymatic digestion in blood, thereby inhibiting ACE, suggesting that food-derived collagen peptides are related to antihypertensive effects.
    No preview · Article · Jan 2009 · Nippon Shokuhin Kagaku Kogaku Kaishi

  • No preview · Article · Jan 2009 · Nihon Chikusan Gakkaiho
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    ABSTRACT: Elastic fibers in the dermis play an important role in skin elasticity. The desmosine crosslinking structure constructed of lysyl oxidase (LOX) in elastic fibers contributes to elasticity, while elastic fibers are primarily degraded by one of the matrix metalloproteinases (MMPs), MMP-12. We investigated the gender differences and diurnal variation of these enzymes. Gender-based differences in LOX mRNA expression were detected, and were significantly lower in females. In contrast, higher MMP-12 mRNA expression was observed in the light period, suggesting that elastic fibers might be degraded in the light rather than the dark period.
    No preview · Article · Dec 2008 · Bioscience Biotechnology and Biochemistry