Luciano Scarponi

Università degli Studi di Perugia, Perugia, Umbria, Italy

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Publications (60)119.03 Total impact

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    Daniele Del Buono · Gerardina Ioli · Luciano Scarponi
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    ABSTRACT: To evidence a possible mechanism of defense toward oxidative stress induced by herbicides in plants, an investigation was carried on the activity of glutathione peroxidase (GPX) in Italian ryegrass (Lolium multiflorum) and in Festuca (Festuca arundinacea) in response to atrazine (6-chloro-N-ethyl-N'-isopropyl-1,3,5-triazine-2,4-diamine) and fluorodifen (4-nitrophenyl α,α,α-trifluoro-2-nitro-p-tolyl ether). In general, the herbicide treatments significantly induced GPX activity in the shoots of Italian ryegrass, whereas inhibited it in Festuca. These opposite behaviors are examined, taking into account the accumulation and persistence of the two herbicides in the plants, and they are discussed in terms of GPX counteraction to oxidative stress in the first case, and of a lower detoxification rate unable to prevent a deleterious effect on the GPX activity in the second case. Further information on the properties of Italian ryegrass and Festuca GPX were achieved by purification and isolation of the enzymes, performed by protein liquid chromatography and by electrophoretic analyses. GPX of both the plants were found to be heterodimer with multiple function in showing also glutathione S-transferase (GST) activity.
    Full-text · Article · Nov 2011 · Journal of Environmental Science and Health Part B Pesticides Food Contaminants and Agricultural Wastes
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    Luciano Scarponi · Daniele Del Buono
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    ABSTRACT: The expression of glutathione S-transferase (GST) activity in Festuca arundinacea was investigated in response to the following herbicide safeners: benoxacor, cloquintocet-mexyl, fenchlorazol-ethyl, fenclorim, fluxofenim and oxabetrinil. All the above compounds enhanced the GST activity tested towards the "model" substrate 1-chloro-2,4-dinitrobenzene (CDNB). Assays of GST activity towards the herbicides terbuthylazine (N(2)-tert-butyl-6-chloro-N(4)-ethyl-1,3,5-triazine-2,4-diamine) and butachlor (N-butoxymethyl-2-chloro-2',6'-diethylacetanilide) as substrates also showed the ability of the safeners to enhance the enzyme activity towards both these herbicides, with the exception of cloquintocet-mexyl for the enzyme activity towards butachlor. As a consequence of the above effects at a macro-scale level, decreased herbicide accumulation and persistence were ascertained in response to the addition of the safener benoxacor to both terbuthylazine and butachlor treatments. These results are discussed in terms of capacity of benoxacor to induce herbicide detoxification in Festuca arundinacea with a view to utilizing them in reducing herbicide pollution.
    Full-text · Article · Nov 2009 · Journal of Environmental Science and Health Part B Pesticides Food Contaminants and Agricultural Wastes
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    Daniele Del Buono · Bhakti Prinsi · Luca Espen · Luciano Scarponi
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    ABSTRACT: The effect of treatments with four herbicides and a safener on the activity of triosephosphate isomerase (TPI) extracted from shoots of Italian ryegrass was investigated. It was found that atrazine and fluorodifen, herbicides which interfere with photosynthesis, caused a decrease in measured enzyme activity. In addition, the in vitro effect of oxidized glutathione (GSSG), a compound produced in situations of oxidative stress, on TPI activity was investigated. It was shown that GSSG was a strong inhibitor of enzyme activity, at low concentrations in a dose-time-dependent manner. The enzyme extracts were submitted to chromatographic purifications and to two-dimensional electrophoresis. Some spots had molecular masses ranging between 20 and 30 kDa and were characterized and identified by LC-ESI-MS/MS as TPIs. The mass spectrometry also made it possible to identify the presence of cysteine residues that could be subjected to S-glutathionylation, which regulate the enzyme activity.
    Full-text · Article · Sep 2009 · Journal of Agricultural and Food Chemistry
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    ABSTRACT: Buffer strips are uncultivated zones left along the boundaries of crops. Buffer strips are used to eliminate or reduce the environmental impact of herbicides. As the efficiency of buffer strips is improved by the presence of non-crop vegetation, we studied the possible role of growing the perennial grass Festuca arundinacea. We investigated the activity in festuca of glutathione S-transferase (GST), which is an enzyme very active in metabolising herbicides. Our results evidence GST activity, which is enhanced by the following compounds: benoxacor, cloquintocet-mexyl, fenchlorazol-ethyl, fenclorim, fluxofenim and oxabetrinil. These synthetic compounds are named herbicide safeners because they protect crop plants against injury from some herbicides without reducing the action of herbicides against the target weeds. The increases in GST activity were found to be concomitant with changes in Vmax and KM, that are kinetic constants related directly to the enzyme concentration in the protein “pull” and inversely to the substrate-enzyme affinity, respectively. In particular, Vmax increase with KM decrease was observed in response to benoxacor, Vmax increases were found in response to fenchlorazol-ethyl, fenclorim, fluxofenim and oxabetrinil, and KM decrease was observed in response to cloquintocet-mexyl. The GST activity was also found to be enhanced by the safeners when it was tested toward the herbicides terbuthylazine and butachlor as substrates. In particular, the increases in GST toward terbuthylazine ranged in the following decreasing order: 154.6%, 91.7%, 89.2%, 88.3%, 82.5% and 30.8% in response to fluxofenim, fenchlorazol-ethyl, fenclorim, oxabetrinil, benoxacor and cloquintocet-mexyl, respectively. The increases in GST toward butachlor ranged in the following decreasing order: 77.0%, 71.2% 59.0%, 41.0% and 33.1% in response to oxabetrinil, benoxacor, fenclorim, fluxofenim and fenchlorazol-ethyl, respectively. A further test, performed to evaluate the relevance of the above effects on a macro-scale level, evidenced 10.1% and 32.7% increased amounts of metabolised terbuthylazine and butachlor, respectively, in response to the addition of benoxacor safener to the herbicide treatments. These results indicate that herbicide diffusion following the runoff of surface waters can be prevented or significantly reduced by vegetating buffer strips with festuca and by the combination of herbicide and a suitable safener. Festuca arundinacea –glutathione S-transferase–terbuthylazine–butachlor–safeners–detoxification–buffer strips
    Full-text · Article · Apr 2009 · Agronomy for Sustainable Development
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    Daniele Del Buono · Luciano Scarponi · Luca Espen
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    ABSTRACT: Over recent years it has emerged how certain no crop-species can be employed in phytoremediating contaminated soils or preventing herbicide pollution; in this contest Festuca arundinacea was investigated. Shoots of Festuca were submitted to fast protein liquid chromatography in order to identify their glutathione S-transferases (GST; EC 2.5.1.18), by a combination of anionic, affinity and RP-HPLC chromatography. The chromatographic procedure revealed satisfactory yield and four GSTs were identified: they were named FaGST I, FaGST II, FaGST III and FaGST IV. Among these, significant differences were observed in the chromatographic behaviours, structure, activity toward a "model" substrate, 1-chloro-2,4-dinitrobenzene, and responsiveness to the herbicide safener benoxacor. FaGST I showed the highest activity toward the above substrate, and this activity was up-regulated by the herbicide safener. Therefore, FaGST I was purified till homogeneity and was determined to be an heterodimer consisting of two subunits of 28.0 and 27.2kDa. Each subunit of FaGST I was further characterized by means of LC-ESI-MS/MS and immunoblotting analysis, which revealed that both the subunits belong to the tau subclass.
    Full-text · Article · Dec 2007 · Phytochemistry
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    Luciano Scarponi · Elisa Quagliarini · Daniele Del Buono
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    ABSTRACT: The expression of glutathione S-transferase (GST) activity in wheat and maize shoots was investigated in response to treatments with the herbicide safeners benoxacor, cloquintocet-mexyl, fenchlorazole-ethyl, fenclorim, fluxofenim and oxabetrinil. These safeners significantly enhanced the GST activity towards 1-chloro-2,4-dinitrobenzene (CDNB) as a 'standard' substrate, with the exception of oxabetrinil in maize. The enhancements of GST (CDNB) activity were found to be concomitant with increases in V(max) (the reaction rate when the enzyme is fully saturated by the substrate) in wheat following cloquintocet-mexyl and fenchlorazole-ethyl treatments, and in maize following fenchlorazole-ethyl treatment. Otherwise, decreases in V(max) were observed in wheat and maize following fenclorim and fluxofenim treatments. With the exception of oxabetrinil, all the safeners significantly reduced the apparent K(M) (the substrate concentration required for 50% of maximum GST activity) of both wheat and maize GST. The V(max) and K(M) variations following safener treatments are discussed in terms of an increased expression of GST enzymes and an increased affinity for the CDNB substrate. The activity of wheat and maize GST was also assayed towards butachlor and terbuthylazine respectively; the results indicate the ability of cloquintocet-mexyl, fenchlorazole-ethyl and fluxofenim to enhance the enzyme activity in wheat and of benoxacor and fenchlorazole-ethyl to do so in maize.
    Full-text · Article · Oct 2006 · Pest Management Science
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    ABSTRACT: A procedure was developed to obtain non-embryogenic callus and regenerated lines from root segments of Zea mays grown in aseptic conditions. The activity of glutathione-S-transferases (GSTs), for non-embryogenic callus, was determined toward 1-chloro-2,4-dinitrobenzene (CDNB) and it was compared with that obtained for corn seedlings grown without hormones. For the callus masses, increases of specific activity toward CDNB and the kinetic parameter Vmax were observed with respect to corn seedlings. The procedure permitted the regenerating of tissues from callus explants, therefore the GST(CDNB) activity and the effect of the safener benoxacor on its expression were investigated for the regenerated tissues grown in agarized substrate and in liquid medium. These explants showed a constitutive GST(CDNB) activity higher than corn seedlings and this activity was increased, for both tissues, in response to the presence of the safener benoxacor in the growing medium. The GST activity for the above tissues was also assayed toward benoxacor and terbuthylazine, metolachlor and fluorodifen herbicides. Measurable GST activity was found toward some of the above chemicals and it was found to be significantly enhanced in response to benoxacor treatment.
    Full-text · Article · Jun 2006 · Pesticide Biochemistry and Physiology
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    ABSTRACT: Herbicide runoff and the effects of a narrow vegetative filter strip (VFS) were studied on an arable field in the low-lying plains of the Veneto Region (north-east Italy). Cultivated plots were compared with and without a 6m wide VFS composed of trees, shrubs and grass. Natural and simulated runoff were monitored during 2000 and 2001. Herbicides applied on the field were: metolachlor (2184-2254 g ha(-1)), terbuthylazine (1000-1127 g ha(-1)) and isoproturon (1000 g ha(-1)). The VFS reduced both runoff depth (10.2-91.2%) and herbicide losses (85.7-97.9%) in the monitored rainfall events. Total herbicide loss with runoff was low (0.69-3.98 g ha(-1) without VFS, less than 0.27 g ha(-1) with VFS), but concentrations were sometimes very high, especially of terbuthylazine and isoproturon during the first events after treatment. In these events there was a high probability of exceeding the ecotoxicological endpoint for algae, but the VFS helped to reduce the potential risk. Two VFS effectiveness mechanisms were identified: (i) dilution, and (ii) a "sponge-like" effect, which temporarily trapped chemicals inside the VFS before releasing them.
    Preview · Article · Dec 2005 · Chemosphere
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    Daniele Del Buono · Luciano Scarponi · Roberto D'Amato
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    ABSTRACT: Butachlor is a chloroacetanilide herbicide successfully employed in weeding some important crops, and benoxacor is a safening compound able to induce the enzymatic mechanism of chloroacetanilide detoxification in plants. A practical method for a simultaneous detection of butachlor and benoxacor residues in wheat and in soil is described. The procedure can be performed by GC and HPLC. They were extracted with methanol and cleaned up by solid phase extraction (SPE). The analytes were satisfactorily separated via both GC and HPLC techniques, and no interferences were observed coming from plant or soil matrixes or reagents. The limit of quantitation was found to be 5.0 ng by GC and 20.0 ng by HPLC for butachlor and 2.5 ng by GC and 15.0 ng by HPLC for benoxacor. Butachlor recovery tests ranged from 85.4% to 91.7% in wheat shoots and 84.0% to 93.2% in soil; benoxacor recovery tests ranged from 86.5% to 90.8% in wheat shoots and 85.7% to 90.7% in soil. The reproducibility and the accuracy make this method a selective and sensitive tool for routine analyses.
    Full-text · Article · Jul 2005 · Journal of Agricultural and Food Chemistry
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    Luciano Scarponi · Daniele Del Buono
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    ABSTRACT: The action of safener benoxacor on the detoxification of terbuthylazine (TBA) in Zea mays and Festuca arundinacea was ascertained by the investigation of the effects of benoxacor on the activity of glutathione-S-transferases (GSTs) in the shoots of the two plant species. TBA treatment generally reduced GST activity toward 1-chloro-2,4-dinitrobenzene (CDNB) in corn and did not affect the enzyme activity in festuca. When applied alone, benoxacor stimulated GST activity in both plants; however, when it was applied in mixture with TBA, generally, an enhancement of the enzyme activity was found in corn but not in festuca in comparison with the respective TBA-treated samples. The enhancement of GST activity in response to the benoxacor treatment in both corn and festuca resulted to be concomitant with decreases in apparent K(M) in both the plant species, with V(max) unaffected, and with an increased expression of proteins having molecular masses in the characteristic range of plant GSTs. After the benoxacor treatment, increased GST activity toward TBA as a substrate was observed in both corn and festuca. As a consequence, lesser amounts and persistence of TBA residues were found in shoots of corn and festuca treated with the TBA and benoxacor mixture compared to TBA-only-treated samples. Therefore, benoxacor enhances TBA detoxification in both corn and festuca; the induction of detoxifying activity in a nondomesticated grass is discussed in view of its use in vegetating buffer strips around crops to prevent TBA pollution.
    Full-text · Article · May 2005 · Journal of Agricultural and Food Chemistry
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    Luciano Scarponi · Daniele Del Buono · Costantino Vischetti
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    ABSTRACT: The persistence of pretilachlor applied to rice singly or in combination with the safener fenclorim was investigated in connection with starch, glucose and protein formation. The addition of fenclorim to pretilachlor did not reduce the accumulation of the latter, but reduced its persistence in rice shoots, while the presence of pretilachlor did not affect the persistence of fenclorim, but significantly increased its accumulation in the shoots. Therefore the safening effect of fenclorim consisted of a more rapid detoxification of pretilachlor. Over the period of pretilachlor and fenclorim detoxification, decreases in starch content, partially counterbalanced by increases of free glucose, and decreases in total protein content were observed in pretilachlor-treated shoots; decreases in both starch and free glucose, as well as in total protein content, were observed in fenclorim -treated shoots compared with the untreated controls. The decreases in starch and total glucose appeared to be a direct consequence of reduced glucokinase and ribulose-1,5-bisphosphate carboxylase activity, and the decrease in total protein an indirect consequence of reduced glutamine synthetase and glutamate synthase activity, in response to pretilachlor and fenclorim treatments.
    Full-text · Article · Apr 2005 · Pest Management Science
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    ABSTRACT: A tool has been designed to calculate the leaching potential of pesticide on a catchment scale using the one-dimensional pesticide leaching model MACRO. The tool is able to collect the input data of the study area in a grid with homogeneous cells and prepare spatially distributed data required by MACRO. Successively, the tool can run multiple MACRO simulations for each cell of the grid and organise output reports following the user's preference, as well as generate output files compatible with GIS and other environmental software applications. The tool was tested to simulate the behaviour in the unsaturated zone of water and pesticide isoproturon used on winter wheat at 1.75 kg ha(-1) of active ingredient in a German study area for 10 years of meteorological data and for different crop-soil combinations. Maps of isoproturon concentration in the liquid phase were created using the spatialised output data from the tool.
    Full-text · Article · Apr 2005 · Agronomy for Sustainable Development
  • Vischetti C · Del Buono D · Scarponi L
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    ABSTRACT: HPLC method to determine simultaneously pretilachlor and fenclorim in soil and in rice plants is reported. The determination is also carried out by GC and GC-MS technique for the identification of the analytes. The method allows for a rapid and quantitative detection of the two compounds both in soil and rice plants. The limit of detection was 5 ng for HPLC and 10 ng for GC. Recoveries were satisfactory and varied from 79.9% to 93.0% in soil and 77.1% to 95.8% in rice plants. Their reproducibility and accuracy make these methods a selective and sensitive tool for routine analyses.
    No preview · Article · Sep 2003 · Fresenius Environmental Bulletin
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    Luciano Scarponi · Daniele Del Buono · Costantino Vischetti
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    ABSTRACT: The contents and persistence of pretilachlor (P) and of the safener fenclorim (F) in rice treated with P, F and their mixture were investigated in relation to the expression of glutathione-S-transferase (GST) activity in response to treatments. F failed to reduce the accumulation of P, however it was able to reduce its persistence in rice shoots. On the other hand, while P did not reduced F persistence, it determined a greater than two-fold F accumulation in plants. Therefore, a detoxification mechanism common to the two compounds but favourable to P was hypothesised. This mechanism consists in an activation of GST activity toward P as substrate [GST(P)] in response to all treatments. The activation levels were found to be proportional to the amount of persistent P and F, and to be additive following the P-F mixture treatment. The kinetic parameters V max and K m of GST(P) were increased and decreased, respectively, in rice shoots treated with P, F and particularly with P-F mixture. Therefore, a defence mechanism toward P, consisting in an increased expression of GST(P) in rice plant, both as the global amount of the enzyme in the protein "pull" and the induction of the enzyme isoforms more sensitive to P, in response to all treatments, especially the P-F mixture treatment, is confirmed. This finding is consistent with the observed effect of F on P persistence and detoxification in the rice shoots. Oryza sativa / pretilachlor / fenclorim / persistence / glutathione-S-transferase Résumé – Persistance et détoxication du pretilachlore et du fenclorim dans le riz (Oryza sativa). On a étudié la teneur et la persistence du pretilachlore (P) et du fenclorim (F), un antidote, dans le riz traité avec P, F et un mélange des deux par référence à l'activité de la glutathion-S-transférase (GST) en réponse aux traitements en question. F n'a pas réussi à réduire l'accumulation de P, mais a pu réduire sa persistance dans les pousses de riz. D'autre part, P n'a pas réduit la persistance de F, mais a déterminé une accumulation plus que double de F dans les plantes. Donc a-t-on soulevé l'hypothèse d'un mécanisme de détoxication commun aux deux composés, mais favorable à P. Ce mécanisme consiste en une activation de l'activité GST à l'égard de P comme substrat [GST(P)] en réponse à tous les traitements. On a relevé que les niveaux d'activation étaient proportionnels à la quantité de P et F persistants, et additifs dans le cas du traitement avec le mélange P-F. Les paramètres cinétiques V max et K m de [GST(P)] ont été respectivement augmentés et diminués dans les pousses de riz traitées avec P, F et particulièrement avec le mélange P-F. On a eu donc confirmation d'un mécanisme de défense à l'égard de P, consistant en une expression accrue de [GST(P)] dans la plante de riz, en ce qui concerne tant la quantité globale de l'enzyme dans le «pull» protéique que l'induction des isoformes enzymatiques plus sensibles à P, en réponse à tous les traitements et surtout au traitement à base de mélange P-F. Ce résultat concorde avec ce qui a été observé quant à l'effet de F sur la persistance et la détoxication de P dans les pousses de riz. Oryza sativa / pretilachlore / fenclorim / persistence / glutathion-S-transférase
    Full-text · Dataset · Jun 2003
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    Luciano Scarponi · Daniele Del Buono · Costantino Vischetti

    Full-text · Article · May 2003 · Agronomy for Sustainable Development
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    Luciano Scarponi · Daniele Del Buono · Costantino Vischetti
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    ABSTRACT: The contents and persistence of pretilachlor (P) and of the safener fenclorim (F) in rice treated with P, F and their mixture were investigated in relation to the expression of glutathione-S-transferase (GST) activity in response to treatments. F failed to reduce the accumulation of P, however it was able to reduce its persistence in rice shoots. On the other hand, while P did not reduced F persistence, it determined a greater than two-fold F accumulation in plants. Therefore, a detoxification mechanism common to the two compounds but favourable to P was hypothesised. This mechanism consists in an activation of GST activity toward P as substrate [GST(P)] in response to all treatments. The activation levels were found to be proportional to the amount of persistent P and F, and to be additive following the P-F mixture treatment. The kinetic parameters V-max and K-m of GST(P) were increased and decreased, respectively, in rice shoots treated with P, F and particularly with P-F mixture. Therefore, a defence mechanism toward P, consisting in an increased expression of GST(P) in rice plant, both as the global amount of the enzyme in the protein "pull" and the induction of the enzyme isoforms more sensitive to P, in response to all treatments, especially the P-F mixture treatment, is confirmed. This finding is consistent with the observed effect of F on P persistence and detoxification in the rice shoots.
    Preview · Article · May 2003 · Agronomie
  • L. Scarponi · C. Vischetti · N.M. Hassan
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    ABSTRACT: The paper describes an experiment undertaken to ascertain the effects of Propachlor (Pr) on the formation of carbohydrates and proteins in Vicia faba, and to evaluate the level of tolerance to the herbicide by verifying a possible defence mechanism of the plant. In response to treatment with three doses of Pr (recommended field dose -f.d.-, half f.d. and double f.d.) significant decreases of total soluble carbohydrates, starch and total protein, which were proportional to the applied dosage, were observed in the shoots during a 16 day period following treatment. This was correlated to reductions in the extractable activity of ribulose 1,5 bisphosphate carboxylase (EC 4.1.1.39) as well as of glutamine synthetase (EC 6.3.1.2) and glutamate synthase (EC 1.4.1.14), which were monitored as parameters of CO2 and ammonia-N assimilation, respectively. Despite these effects, the seedlings managed to survive with an almost negligible reduction in dry weight. This seems to be due to the herbicide-induced activity of glutathione S-transferase (EC 2.5.1.18), which was checked as a parameter of the capacity of the plant to detoxify the herbicide through conjugation with glutathione or homoglutathione.
    No preview · Article · May 2002 · Agrochimica -Pisa-
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    ABSTRACT: The capability to predict the fate of pesticides in a southern Italian scenario was evaluated for three Pesticide Leaching Models (PLMs) (VARLEACH, PELMO and PESTLA), using a data set previously achieved for this purpose. The measurement of actual evapotranspiration in field experiments allowed a calibration of the three PLMs for the hydrological component in the scenario studied, where evapotranspiration was very high in the hotter months. While PELMO and PESTLA were easily calibrated, VARLEACH did not give satisfactory results, maybe due to its intrinsic deficiency. After calibration, the three PLMs simulated well the movement of the two herbicides imazethapyr and bentazone along the soil profile. Agreement could be casual for VARLEACH, since it did not simulate well the movement of water and bromide (non-interfering ion). PESTLA simulated well the total herbicide amount leached below 80 cm depth, while VARLEACH and PELMO failed at this. The three calibrated PLMs could be used to perform simulations in the same climatic conditions as the experiment.
    No preview · Article · May 2002 · Agronomie
  • Scarponi L · Vischetti C · Del Buono D

    No preview · Article · Mar 2002 · Italian Journal of Food Science
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    ABSTRACT: The ability of acibenzolar-S-methyl to induce resistance in pepper plants against Xanthomonas campestris pv. vesicatoria was investigated in both growth chamber and open field conditions. Growth chamber experiments showed that acibenzolar-S-methyl (300M) treatment protects pepper plants systemically and locally against X. campestris pv. vesicatoria. Evidence for this was a reduction in the number and diameter of bacterial spots and bacterial growth in planta. Systemic protection was also exerted by the acibenzolar-S-methyl acid derivative, CGA 210007, which may be produced by hydrolysis in the plant. The efficacy of acibenzolar-S-methyl was also found in open field conditions, where both leaves and fruit were protected from the disease. The highest efficacy (about 67%) was obtained by spraying the plants 6–7 times every 8–12 days with a mixture of acibenzolar-S-methyl and copper hydroxide (2.5 + 40ghl–1 active ingredient). Persistence and translocation data obtained from the growth chamber experiments revealed a persistence of acibenzolar-S-methyl lasting five days after treatment with rapid translocation and negligible levels of acid derivative formation. Since the protection exerted by acibenzolar-S-methyl against bacterial spot disease was observed when the inducer was completely degraded, it would appear to be due to SAR activation.
    Full-text · Article · Dec 2001 · European Journal of Plant Pathology

Publication Stats

577 Citations
119.03 Total Impact Points

Institutions

  • 1975-2011
    • Università degli Studi di Perugia
      • • Department of Environmental and Agricultural Sciences
      • • Faculty of Agriculture
      Perugia, Umbria, Italy
  • 2002
    • Mansoura University
      • Department of Botany
      El-Manṣûra, Muḩāfaz̧at ad Daqahlīyah, Egypt
  • 1988
    • Università degli Studi della Basilicata
      Potenza, Basilicate, Italy