Thomas F Baumert

Institut de Recherche contre les Cancers de l'Appareil Digestif, Strasburg, Alsace, France

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Publications (272)2055.2 Total impact

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    ABSTRACT: Following the discovery of the hepatitis C virus (HCV) more than 25 years ago the field has succeeded to develop methods that have changed the safety of blood products, understand the molecular virology, epidemiology and clinical disease of HCV, and identify specific targets for the development of direct-acting antivirals for HCV cure. Nevertheless major clinical and scientific challenges remain: Therapy is still only available to a fraction of infected patients worldwide and many patients remain undiagnosed and/or live in countries where therapy is unattainable. An urgently needed HCV vaccine to eradicate infection remains still elusive. Scientifically, major questions remain regarding the life cycle, pathogenesis and mechanisms of viral clearance and persistence. Addressing these challenges, this meeting report reviews key findings of the 22nd International Symposium on Hepatitis C Virus and Related Viruses in Strasbourg, France from October 9 to 13, 2015.
    No preview · Article · Jan 2016 · Journal of Hepatology
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    ABSTRACT: Conclusion: Although mAbs AP33 and 3/11 target the same region in E2, only mAb AP33 can efficiently protect from challenge with a heterologous HCV population in vivo. Since mAb AP33 efficiently neutralizes viral variants that escaped the humoral immune response and re-infected the liver graft of transplant patients, it may be a valuable candidate to prevent HCV recurrence. In addition our data is valuable for the design of a prophylactic vaccine. This article is protected by copyright. All rights reserved.
    No preview · Article · Dec 2015 · Hepatology
  • Laurent Mailly · Mirjam B Zeisel · Thomas F Baumert

    No preview · Article · Dec 2015
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    ABSTRACT: Chronic hepatitis C virus (HCV) infection is a major cause of liver cirrhosis and hepatocellular carcinoma (HCC) which are leading indications of liver transplantation (LT). To date, there is no vaccine to prevent HCV infection and LT is invariably followed by infection of the liver graft. Within the past years, direct-acting antivirals (DAAs) have had a major impact on the management of chronic hepatitis C, which has become a curable disease in the majority of DAA-treated patients. In contrast to DAAs that target viral proteins, host-targeting agents (HTAs) interfere with cellular factors involved in the viral life cycle. By acting through a complementary mechanism of action and by exhibiting a generally higher barrier to resistance, HTAs offer a prospective option to prevent and treat viral resistance. Indeed, given their complementary mechanism of action, HTAs and DAAs can act in a synergistic manner to reduce viral loads. This review summarizes the different classes of HTAs against HCV infection that are in preclinical or clinical development and highlights their potential to prevent HCV infection, e.g., following LT, and to tailor combination treatments to cure chronic HCV infection.
    Preview · Article · Nov 2015 · Viruses
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    ABSTRACT: Background & aims: Follicular T-helper (Tfh) cells contribute to pathogen-specific antibody responses by providing maturation signals to B cells. In mice with viral infections, virus-specific Tfh cells expand and are required to contain the infection. However, less is known about human virus-specific Tfh cells or their functions during infection. We investigated whether virus-specific CD4+ T cells from patients with HCV infection had phenotypic or functional features of Tfh cells and contribute to the production of HCV-specific antibodies METHODS: We collected blood samples from patients with acute and chronic HCV infection and healthy individuals (controls). We performed MHC class II tetramer analyses, assays to detect intracellular cytokines in response to HCV exposure, and analyses to quantify HCV-specific antibodies. In addition, we collected liver tissues from patients with chronic HCV infection or non-viral liver disease to analyze markers of Tfh cells. Results: HCV-specific CD4+ T cells from patients with acute HCV infection expressed markers of Tfh cells and secreted interleukin-21 in response to HCV exposure. Longitudinal analyses of HCV-specific T-cell responses and antibody responses revealed an association between expression of inducible T cell co-stimulator (ICOS) and induction of virus-specific antibodies in patients with acute HCV infection. Markers of Tfh cells were barely detectable in the peripheral blood samples from patients with chronic HCV infection, but were detected in liver tissues. Conclusions: Virus-specific Tfh cells can be detected in blood samples from patients with acute HCV infection; ICOS expression correlates with production of HCV-specific antibodies. In patients with chronic infection, Tfh cells seem to disappear from the blood but are detectable in the liver.
    No preview · Article · Nov 2015 · Gastroenterology
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    Full-text · Dataset · Oct 2015
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    ABSTRACT: Hepatitis B virus (HBV) is one of the world's major unconquered infections, resulting in progressive liver disease and current treatments rarely cure infection. A major limitation to discovering new therapies is our limited knowledge of HBV entry and dissemination pathways that hinders the development of in vitro culture systems. To address this gap in our understanding we optimised the genesis of infectious lentiviral pseudoparticles (HBVpp). The recent discovery that the bile salt transporter NTCP acts as a receptor for HBV enabled us to assess the receptor dependency of HBVpp infection. HBVpp preferentially infect hepatoma cells expressing NTCP, whereas other non-liver cells engineered to express NTCP do not support infection, suggesting that additional hepatocyte-specific factors are required for HBVpp internalisation. These results highlight the value of the HBVpp system to dissect the pathways of HBV entry and dissemination.
    Full-text · Article · Oct 2015 · Journal of General Virology
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    ABSTRACT: We evaluated the possibility of introducing a combination of six oncogenes into primary porcine hepatocytes (PPH) using a lentiviral vector (LV)-mediated gene transfer in order to develop a porcine hepatocellular carcinoma (HCC) model based on autologous transplantation of ex vivo-transformed hepatocytes. The six oncogenes were introduced into three plasmids, hence enabling the production of LVs encoding a luciferase reporter gene and hTERT+p53DD, cyclinD1+CDK4R24C, and c-mycT58A+HRasG21V genes respectively. In order to improve the protection of the laboratory personnel manipulating such LVs, we used a compact cell culture cassette, (CliniCell® device) as a closed cell culture system. We demonstrated that the CliniCell® device allows to produce lentiviral vectors, through plasmid transfection of 293T cells, and, after transfer to a second cassette, to transduce PPH with a similar efficacy as conventional open cell culture systems such as flasks or Petri dishes. Additionally, it is possible to cryopreserve at minus 80°C the transduced cells, directly in the CliniCell® device used for the transduction. In conclusion, the use of a closed culture system for the safe handling of oncogene-encoding LVs lays the foundation for the development of porcine tumor models based on the autologous transplantation of ex vivo-transformed primary cells.
    No preview · Article · Oct 2015 · Human Gene Therapy Methods
  • Che C Colpitts · Thomas F Baumert
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    ABSTRACT: Despite advances in hepatitis C treatment, substantial clinical hurdles remain to achieve universal cure and global control of infection. Saeed et al. identified SEC14L2 as a host factor permitting replication of clinical HCV isolates in cell culture, providing a novel system to model infection of patient-derived viruses.
    No preview · Article · Oct 2015 · Nature Reviews Gastroenterology &#38 Hepatology
  • Joachim Lupberger · Nicolaas Van Renne · Thomas F. Baumert

    No preview · Chapter · Oct 2015
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    ABSTRACT: Hepatitis C virus (HCV) remains a major global health burden, with more than 130 million individuals chronically infected and at risk for the development of hepatocellular carcinoma (HCC). The recent clinical licensing of direct-acting antivirals enables viral cure. However, limited access to therapy and treatment failure in patient subgroups warrants a continuing effort to develop complementary antiviral strategies. Furthermore, once fibrosis is established, curing HCV infection does not eliminate the risk for HCC. High-throughput approaches and screens have enabled the investigation of virus-host interactions on a genome-wide scale. Gain- and loss-of-function screens have identified essential host-dependency factors in the HCV viral life cycle, such as host cell entry factors or regulatory factors for viral replication and assembly. Network analyses of systems-scale data sets provided a comprehensive view of the cellular state following HCV infection, thus improving our understanding of the virus-induced responses of the target cell. Interactome, metabolomics and gene expression studies identified dysregulated cellular processes potentially contributing to HCV pathogenesis and HCC. Drug screens using chemical libraries led to the discovery of novel antivirals. Here, we review the contribution of high-throughput approaches for the investigation of virus-host interactions, viral pathogenesis and drug discovery.
    Full-text · Article · Sep 2015 · Virus Research
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    ABSTRACT: Background & aims: Efforts to develop an effective vaccine against hepatitis C virus (HCV) have been hindered by the propensity of the virus to evade host immune responses. HCV particles in serum and in cell culture (HCVcc) associate with lipoproteins, which contribute to viral entry. Lipoprotein association has also been proposed to mediate viral evasion of the humoral immune response, though the mechanisms are poorly defined. Methods: We used small interfering RNAs to reduce levels of apolipoprotein E (APOE) in HCVcc-producing Huh7.5-derived hepatoma cells and confirmed its depletion by immunoblot analyses of purified viral particles. Before infecting infection of naïve hepatoma cells, we exposed HCVcc strains of different genotypes, subtypes, and variants to serum and polyclonal and monoclonal antibodies isolated from patients with chronic HCV infection. We analyzed the interaction of APOE with viral envelope glycoprotein E2 and HCV virions by immunoprecipitation. Results: Through loss-of-function studies on patient-derived HCV variants of several genotypes and subtypes, we found that the HCV particle APOE allows the virus to avoid neutralization by patient-derived antibodies. Functional studies with human monoclonal antiviral antibodies showed that conformational epitopes of E2 envelope glycoprotein domains B and C were exposed following depletion of APOE. The level and conformation of virion-associated APOE affected the ability of the virus to escape neutralization by antibodies. Conclusions: In cell infection studies, we found that HCV-associated APOE helps the virus avoid neutralization by antibodies against HCV isolated from chronically infected patients. This method of immune evasion poses a challenge for the development of HCV vaccines.
    No preview · Article · Sep 2015 · Gastroenterology
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    ABSTRACT: Cellular translation is down-regulated by host antiviral responses. Picornaviridae and Flaviviridae including hepatitis C virus (HCV) evade this process using internal ribosomal entry sequences (IRESs). Although HCV IRES translation is a prerequisite for HCV replication, only few host factors critical for IRES activity are known and the global regulator network remains largely unknown. Since signal transduction is an import regulator of viral infections and the host antiviral response we combined a functional RNAi screen targeting the human signaling network with a HCV IRES-specific reporter mRNA assay. We demonstrate that the HCV host cell cofactors PI4K and MKNK1 are positive regulators of HCV IRES translation representing a novel pathway with a functional relevance for the HCV life cycle and IRES-mediated translation of viral RNA.
    Full-text · Article · Sep 2015 · Scientific Reports
  • Joachim Lupberger · Nicolaas Van Renne · Thomas F. Baumert
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    ABSTRACT: This chapter describes how hepatitis C virus (HCV) interacts with host kinases and modulates the activities of signaling pathways. HCV makes use of signal transduction in order to maintain and promote viral entry, replication, and assembly and to evade the cellular antiviral response. These events contribute to the chronification of HCV infection and virus-induced pathogenesis. A detailed knowledge of virus-induced changes in signaling patterns will make it possible to develop new therapeutic strategies against HCV infection.
    No preview · Article · Aug 2015
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    ABSTRACT: Hepatitis B virus (HBV) infection is a major cause of chronic liver disease, including liver cirrhosis, liver failure and hepatocellular carcinoma (HCC)-the second leading and fastest rising cause of cancer death world-wide. While de novo infection can be efficiently prevented by vaccination and chronic infection can be controlled using antivirals targeting the viral polymerase, the development of efficient antiviral strategies to eliminate the virus and thus to cure infection remains a key unmet medical need. The recent progress in the development of robust infectious HBV cell culture models now enables the investigation of the full viral life cycle, including a more detailed study of the molecular mechanisms of virus-host interactions responsible for viral persistence. The understanding of these virus-host interactions will be instrumental for the development of curative treatments. Host-dependency factors have recently emerged as promising candidates to treat and prevent infection by various pathogens. This review focuses on the potential of host-targeting agents (HTAs) as novel antivirals to treat and cure HBV infection. These include HTAs that inhibit de novo and re-infection, synthesis and spread of cccDNA as well as development of immune-based approaches eliminating or curing infected hepatocytes, including the eradication of viral cccDNA. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Aug 2015
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    ABSTRACT: Lipoprotein components are crucial factors for hepatitis C virus (HCV) assembly and entry. As hepatoma cells producing cell culture-derived HCV particles (HCVcc) are impaired in some aspects of lipoprotein metabolism, it is of upmost interest to biochemically and functionally characterize the in vivo-produced viral particles, particularly regarding how lipoprotein components modulate HCV entry by lipid transfer receptors such as scavenger receptor BI (SR-BI). Sera from HCVcc-infected liver humanized FRG mice were separated by density gradients. Viral subpopulations, termed HCVfrg particles, were characterized for their physical properties, apolipoprotein association and infectivity. We demonstrate that in contrast to the widely-spread distribution of apolipoproteins across the different HCVcc subpopulations, the most infectious HCVfrg particles are highly enriched in ApoE, suggesting that such apolipoprotein enrichment plays a role for entry of in vivo-derived infectious particles, likely via usage of apolipoprotein receptors. Consistent with this salient feature, we further reveal previously undefined functionalities of SR-BI in promoting entry of in vivo-produced HCV. First, unlike HCVcc, SR-BI is a particularly limiting factor for entry of HCVfrg subpopulations of very low density. Second, HCVfrg entry involves SR-BI lipid transfer activity, but not its binding capacity to the viral glycoprotein E2. In conclusion, we demonstrate that composition and biophysical properties of the different subpopulations of in vivo-produced HCVfrg particles modulate their levels of infectivity and receptor usage, hereby featuring divergences with in vitro-produced HCVcc particles and highlighting the powerfulness of this in vivo model for the functional study of the interplay between HCV and liver components. Copyright © 2015, The American Society for Biochemistry and Molecular Biology.
    No preview · Article · Jul 2015 · Journal of Biological Chemistry
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    ABSTRACT: Chronic hepatitis B and D infections are major causes of liver disease and hepatocellular carcinoma worldwide. Efficient therapeutic approaches for cure are absent. Sharing the same envelope proteins, hepatitis B virus (HBV) and hepatitis D virus (HDV) use the sodium taurocholate co-transporting polypeptide (NTCP, a bile acid transporter) as a receptor to enter hepatocytes. However, the detailed mechanisms of the viral entry process are still poorly understood. Here, we established a high-throughput infectious cell culture model enabling functional genomics of HDV entry and infection. Using a targeted RNAi entry screen we identified glypican 5 (GPC5) as a common host cell entry factor for HBV and HDV. These findings advance our understanding of virus cell entry and open new avenues for curative therapies. Since glypicans have been shown to play a role in the control of cell division and growth regulation, virus-GPC5 interactions may also play a role in the pathogenesis of virus-induced liver disease and cancer. This article is protected by copyright. All rights reserved. © 2015 by the American Association for the Study of Liver Diseases.
    Full-text · Article · Jul 2015 · Hepatology
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    ABSTRACT: Hepatitis C virus (HCV) enters human hepatocytes through a multistep mechanism involving, among other host proteins, the virus receptor CD81. How CD81 governs HCV entry is poorly characterized, and CD81 protein interactions after virus binding remain elusive. We have developed a quantitative proteomics protocol to identify HCV-triggered CD81 interactions and found 26 dynamic binding partners. At least six of these proteins promote HCV infection, as indicated by RNAi. We further characterized serum response factor binding protein 1 (SRFBP1), which is recruited to CD81 during HCV uptake and supports HCV infection in hepatoma cells and primary human hepatocytes. SRFBP1 facilitates host cell penetration by all seven HCV genotypes, but not of vesicular stomatitis virus and human coronavirus. Thus, SRFBP1 is an HCV-specific, pan-genotypic host entry factor. These results demonstrate the use of quantitative proteomics to elucidate pathogen entry and underscore the importance of host protein-protein interactions during HCV invasion. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
    Preview · Article · Jul 2015 · Cell Reports
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    Full-text · Dataset · Jun 2015
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    Eloi R Verrier · Stefan Wieland · Thomas F Baumert

    Full-text · Article · Jun 2015 · Hepatology

Publication Stats

7k Citations
2,055.20 Total Impact Points

Institutions

  • 2015
    • Institut de Recherche contre les Cancers de l'Appareil Digestif
      Strasburg, Alsace, France
    • Institut Hospitalo-Universitaire
      Pessac, Aquitaine, France
  • 2014-2015
    • Harvard University
      Cambridge, Massachusetts, United States
  • 2007-2015
    • French Institute of Health and Medical Research
      Lutetia Parisorum, Île-de-France, France
    • University of Strasbourg
      • Laboratoire de BioVectorologie
      Strasburg, Alsace, France
    • Institut Pasteur de Lille
      Lille, Nord-Pas-de-Calais, France
  • 1996-2015
    • Harvard Medical School
      Boston, Massachusetts, United States
    • Massachusetts General Hospital
      • Department of Medicine
      Boston, Massachusetts, United States
  • 2013
    • Imperial College London
      Londinium, England, United Kingdom
    • Institut de France
      Lutetia Parisorum, Île-de-France, France
  • 2012
    • University of Birmingham
      • School of Biosciences
      Birmingham, England, United Kingdom
    • Johns Hopkins University
      • Department of Medicine
      Baltimore, Maryland, United States
  • 2010
    • Stanford University
      • Department of Pathology
      Palo Alto, California, United States
  • 2009-2010
    • Unité Inserm U1077
      Caen, Lower Normandy, France
  • 2001-2009
    • University of Freiburg
      • • Department of Immunology
      • • Faculty of Biology
      • • Department of Internal Medicine
      Freiburg, Baden-Württemberg, Germany
  • 2006-2007
    • Humboldt-Universität zu Berlin
      Berlín, Berlin, Germany
    • Rensselaer Polytechnic Institute
      • Department of Chemical and Biological Engineering
      Troy, New York, United States
  • 2004
    • Technische Universität München
      München, Bavaria, Germany
  • 1998-2001
    • National Institutes of Health
      • National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
      Maryland, United States
  • 2000
    • The National Institute of Diabetes and Digestive and Kidney Diseases
      베서스다, Maryland, United States