Xiumei Mo

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (94)348.94 Total impact

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    ABSTRACT: Biomaterials are playing a significant role in understanding and promoting the plasticity and repair of the nervous system. Biomimetic nanofibrous scaffolds mimicking important features of the native extracellular matrix provide a promising strategy to restore functions or achieve favorable responses for tissue regeneration and autograft nerve conduit is one of the most promising nerve regeneration strategies. The present study is based on novel fabrication method by using a special collector for 3D multichannel nerve conduit, longitudinally oriented with aligned electrospun nanofibers. The conduit contained a high number of channels (varying from 7 to 19) and each channel showed a separate morphology. Nerve channels were fabricated with the varying length ranging from 4 to 9 cm and total diameter ranging from 2200 ± 40 µm to 3951 ± 196 µm, while the channel diameter ranging from 350 ± 86 µm to 780 ± 20 µm. It has been clearly shown that the average porosity of nerve conduits has reached almost 89%. In this study, we optimized the parameters to control the structural stability, including the size and the number of channels in the nerve conduit. We also checked in vitro cell biocompatibility of multichannel nerve conduit and demonstrated that Schwann cells have the tendency to grow along the direction of nanofibers and high cell growth was observed in high number of channels compared to low number of channels. These results elaborated the potential use of this biocompatible multichannel nerve conduit for further in vivo testing.
    No preview · Article · May 2016 · International Journal of Polymeric Materials
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    ABSTRACT: Soft tissue adhesives made from natural hydrogel are attractive in clinical applications due to their excellent properties, such as high water content, good biocompatibility, low immune, good biodegradability. Hydrogels derived from natural polysaccharides and proteins are ideal components for soft tissue adhesive since they resemble the extracellular matrices of the tissue composed of various sugar and amino acids-based macromolecules. In this paper, a series of novel tissue adhesives mixed by aldehyde sodium alginate (ASA) with amino gelatin (AG) were developed and characterized. The effect of aldehyde content in ASA and amino group content in AG on the properties of ASA/AG cross-linked hydrogel was measured. The results showed the gelling time, swelling behavior and the bonding strength of the hydrogel can be tuned by varying the content of aldehyde groups in ASA and the content of amino groups in AG. The gelation time could be controlled within 4-18 min. When the aldehyde content of ASA is 75.24% and the amino content of AG is 0.61 mmol/g, the hydrogel almost has the adhesive strength equal to the commercially available adhesive fibrin glue. So, this tunable ASA/AG hydrogels in this study could be a promising candidate as soft tissue adhesive and have a wide range of biomedical applications.
    No preview · Article · Feb 2016 · Artificial Cells
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    ABSTRACT: The gelatin–glutaraldehyde (gelatin–GA) nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity. The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolytic resistance, mechanical property and biocompatibility of nanofiber scaffolds were tested and characterized. The gelatin–GA nanofiber has nice uniform diameter and more than 80% porosity. The hydrolytic resistance and mechanical property of the gelatin–GA nanofiber scaffolds are greatly improved compared with that of gelatin nanofibers. The contact angle, moisture absorption, hydrolysis resistance, thermal resistance and mechanical property of gelatin–GA nanofiber scaffolds could be adjustable by varying the gelatin solution concentration and GA content. The gelatin–GA nanofibers had excellent properties, which are expected to be an ideal scaffold for biomedical and tissue engineering applications.
    No preview · Article · Jan 2016 · Frontiers of Materials Science
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    ABSTRACT: The incorporation of microcarriers as drug delivery vehicles into polymeric scaffold for bone regeneration has aroused increasing interest. In this study, the aminated mesoporous silica nanoparticles (MSNs-NH2) were prepared and used as microcarriers for dexamethasone (DEX) loading. Poly(L-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) nanofibrous scaffold was fabricated via thermally induced phase separation (TIPS) and served as template, onto which the drug-loaded MSNs-NH2 nanoparticles were deposited by electrophoretic deposition (EPD). The physicochemical and release properties of the prepared scaffolds (DEX@MSNs-NH2/PLLA/PCL) were examined, and their osteogenic activities were also evaluated through in vitro and in vivo studies. The release of DEX from the scaffolds revealed an initial rapid release followed by a slower and sustained one. The in vitro results indicated that the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited good biocompatibility to rat bone marrow-derived mesenchymal stem cells (BMSCs). Also, BMSCs cultured on the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited higher degree of osteogenic differentiation than those cultured on PLLA/PCL and MSNs-NH2/PLLA/PCL scaffolds, in terms of alkaline phosphatase (ALP) activity, mineralized matrix formation and osteocalcin (OCN) expression. Furthermore, the in vivo results in a calvarial defect model of Sprague Dawley (SD) rats demonstrated that the DEX@MSNs-NH2/PLLA/PCL scaffold could significantly promote calvarial defect healing compared with PLLA/PCL scaffold. Thus, the EPD technique provides a convenient way to incorporate osteogenic agents-containing microcarriers to polymer scaffold, and thus prepared composite scaffold could be a potential candidate for bone tissue engineering application due to its capacity for delivery of osteogenic agents.
    No preview · Article · Jan 2016 · ACS Applied Materials & Interfaces
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    ABSTRACT: Electrospun gelatin(Gel) nanofibers scaffold has such defects as poor mechanical property and quick degradation due to high solubility. In this study, the in-situ crosslinked electrospinning technique was used for the production of gelatin nanofibers. Deionized water was chosen as the spinning solvent and Graphite Oxide (GO) was chosen as the enhancer.The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolysis resistance, mechanical property and biocompatibility of the produced nanofibers were investigated. Compared with in-situ crosslinked gelatin nanofibers scaffold, in-situ crosslinked Gel-GO nanofibers scaffold has the following features: (1) the hydrophilicity, moisture absorption and moisture retention performance slightly reduce, while the hydrolysis resistance is improved; (2) the breaking strength, breaking elongation and Young's modulus are significantly improved; (3) the porosity slightly reduces while the biocompatibility considerably increases. The in-situ crosslinked Gel-GO nanofibers scaffold is likely to be applied in such fields as drug delivery and scaffold for skin tissue engineering.
    No preview · Article · Jan 2016 · Journal of Biomaterials Science Polymer Edition
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    ABSTRACT: Injuries of the peripheral nerve occur commonly in various people of different ages and backgrounds. Generally, surgical repairing, such as suturing the transected nerve stumps and transplanting an autologous nerve graft, is the only choice. However, tissue engineering provides an alternative strategy for regeneration of neural context. Functional nerve conduits with three dimensional (3D) support and guidance structure are badly in need. Herein, a uniform PLLA nanofiber yarn constructed by unidirectionally aligned nanofibers was fabricated via a dual spinneret system, which was subsequently incorporated into a hollow poly(l-lactide-co-caprolactone) (P(LLA-CL)) tube to form a nerve conduit with inner aligned texture. The biocompatibility of the poly(l-lactic acid) (PLLA) yarn was assessed by in vitro experiments. Schwann cells (SCs) presented a better proliferation rate and spread morphology of the PLLA yarn than that of PLLA film. Confocal images indicated that the axon spreads along the length of the yarn. SCs were also cultured in the conduit. The data indicated that SCs proliferated well in the conduit and distributed dispersedly throughout the entire lumen. These results demonstrated the potential of the PLLA nanofiber yarn conduit in nerve regeneration.
    No preview · Article · Nov 2015 · Journal of Materials Chemistry B
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    ABSTRACT: Silk fibroin–poly(lactic-co-glycolic acid) fibrous membranes were electrospun by varying the weight ratios for silk fibroin to poly(lactic-co-glycolic acid). The hydrophilicity, mechanical property, and biodegradability of the fibrous in vitro were evaluated. Contact angle test demonstrated that the hydrophilicity of poly(lactic-co-glycolic acid) fibrous membrane could be improved by introducing silk fibroin ingredient. Mechanical test showed that the strain–elongation performances of silk fibroin–poly(lactic-co-glycolic acid) fibrous can be controlled by changing the silk fibroin percentage. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay test showed that the silk fibroin–poly(lactic-co-glycolic acid) 2:8 fibrous enhanced the nerve cell proliferation compared to poly(lactic-co-glycolic acid) fibrous. Silk fibroin–poly(lactic-co-glycolic acid) fibrous membrane has been made into the nerve guidance conduit by the reeling and the sewing processing. The poly(lactic-co-glycolic acid) nerve guidance conduit and silk fibroin–poly(lactic-co-glycolic acid) nerve guidance conduit were implanted into a 10-mm sciatic nerve defect part of mice for nerve regeneration and the nerve regenerated at 12 weeks. Nerve regeneration test showed that the regenerated nerve in the silk fibroin–poly(lactic-co-glycolic acid) nerve guidance conduit group was more organized and mature than that in the poly(lactic-co-glycolic acid) nerve guidance conduit group. The results suggest that the silk fibroin–poly(lactic-co-glycolic acid) (2:8) nerve guidance conduits have potential applications in nerve regeneration.
    No preview · Article · Oct 2015 · Journal of Bioactive and Compatible Polymers
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    ABSTRACT: Restenosis caused by thrombopoiesis is one of the biggest hinders of endovascular stent-graft used in small-diameter vessels. Rapid endothelialization of the lumen of stent is a promising approach to prevent thrombosis. In this study, we aimed at loading heparin, a potent anticoagulants, and vascular endothelial growth factor (VEGF) into the core of poly(L-lactide-co-caprolactone) nanofiber via emulsion electrospinning. The nanofiber was covered on the stent and applied in the treatment of vascular diseases such as aneurysm. The morphologies of the emulsion electrospun nanofibers and core–shell structure were observed by scanning electron microscope and laser scanning confocal microscope. The release profiles of heparin and VEGF, degradation rate of nanofiber mats and cell proliferation in vitro were investigated. It was found that the release of both heparin and VEGF from the nanofiber lasted for more than 30 days without serious initial burst release. The degradation rate of nanofiber mats containing heparin and VEGF was faster than that of pure PLCL nanofiber mats. Moreover, the released VEGF could promote the proliferation of Pig iliac endothelial cells (PIECs) cultured on the nanofiber mat, which was of great benefit to stent endothelialization. The results of digital subtraction angiography (DSA) follow-up indicated the aneurysm was obliterated by separating the aneurysm dome from the blood circulation and the parent artery kept long-term patency. Results of the study demonstrated that the heparin and VEGF loaded nanofiber could provide an approach to fabricate covered stent-graft with properties of anticoagulation and induction of rapid endothelialization.
    Full-text · Article · Oct 2015 · Journal of Biomedical Nanotechnology
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    ABSTRACT: Biocompatible polymer scaffolds are promising as potential carriers for the delivery of retinal progenitor cells (RPCs) in cell replacement therapy for the repair of damaged or diseased retinas. The primary goal of the present study was to investigate the effects of blended electrospun nanofibrous membranes of silk fibroin (SF) and poly(L-lactic acid-co-ε-caprolactone) (PLCL), a novel scaffold, on the biological behaviour of RPCs in vitro. To assess the cell-scaffold interaction, RPCs were cultured on SF/PLCL scaffolds for indicated durations. Our data revealed that all the SF/PLCL scaffolds were thoroughly cytocompatible, and the SF:PLCL (1:1) scaffolds yielded the best RPC growth. The in vitro proliferation assays showed that RPCs proliferated more quickly on the SF:PLCL (1:1) than on the other scaffolds and the control. Quantitative polymerase chain reaction (qPCR) and immunocytochemistry analyses demonstrated that RPCs grown on the SF:PLCL (1:1) scaffolds preferentially differentiated toward retinal neurons, including, most interestingly, photoreceptors. In summary, we demonstrated that the SF:PLCL (1:1) scaffolds can not only markedly promote RPC proliferation with cytocompatibility for RPC growth but also robustly enhance RPCs' differentiation toward specific retinal neurons of interest in vitro, suggesting that SF:PLCL (1:1) scaffolds may have potential applications in retinal cell replacement therapy in the future.
    Preview · Article · Sep 2015 · Scientific Reports
  • Jun Fang · Jing Wang · Tong Wu · Anlin Yin · Xiumei Mo

    No preview · Article · Sep 2015 · Journal of Controlled Release

  • No preview · Article · Sep 2015 · Journal of Controlled Release
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    ABSTRACT: Cartilage defects cause joint pain and loss of mobility. It is crucial to induce the chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by both biological and structural signals in cartilage tissue engineering. Sponge-like scaffolds fabricated using native cartilage extracellular matrix components can induce the BMSC differentiation by biological signals and limited structural signals. In this study, an oriented poly(L-lactic acid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I (Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/hyaluronate (HA) chondral phase (SPONGE) containing both structural and biological signals to guide BMSC chondrogenic differentiation. In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) promotes orientation, adhesion and proliferation of BMSCs better than SPONGE. Furthermore, BMSCs seeded on the Yarn-CH scaffold demonstrated a large increase in the glycosaminoglycan content and expression of collagen type II following a 21-day culture.
    No preview · Article · Aug 2015 · Materials Science and Engineering C
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    ABSTRACT: Bone morphogenetic protein-2 (BMP-2), a growth factor that induces osteoblast differentiation and promotes bone regeneration, has been extensively investigated in bone tissue engineering. The peptides of bioactive domains, corresponding to residues 73-92 of BMP-2 become an alternative to reduce adverse side effects caused by the use of high doses of BMP-2 protein. In this study, BMP-2 peptide functionalized mesoporous silica nanoparticles (MSNs-pep) were synthesized by covalently grafting BMP-2 peptide on the surface of nanoparticles via an aminosilane linker, and dexamethasone (DEX) was then loaded into the channel of MSNs to construct nanoparticulate osteogenic delivery systems (DEX@MSNs-pep). The in vitro cell viability of MSNs-pep was tested with bone mesenchymal stem cells (BMSCs) exposure to different particle concentrations, revealing that the functionalized MSNs had better cytocompatibility than their bare counterparts, and the cellular uptake efficiency of MSNs-pep was remarkably larger than that of bare MSNs. The in vitro results also show that the MSNs-pep promoted osteogenic differentiation of BMSCs in terms of the levels of alkaline phosphatase (ALP) activity, calcium deposition, and expression of bone-related protein. Moreover, the osteogenic differentiation of BMSCs can be further enhanced by incorporating of DEX into MSNs-pep. After intramuscular implantation in rats for three weeks, the computed tomography (CT) images and histological examination indicate that this nanoparticulate osteogenic delivery system induces effective osteoblast differentiation and bone regeneration in vivo. Collectively, the BMP-2 peptide and DEX incorporated MSNs can act synergistically to enhance osteogenic differentiation of BMSCs, which have potential applications in bone tissue engineering.
    No preview · Article · Jul 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: Multi-layered scaffolds are advantageous in vascular tissue engineering, in consideration of better combination of biomechanics, biocompatibility and biodegradability than the scaffolds with single structure. In this study, a bi-directional gradient electrospinning method was developed to fabricate poly(l-lactide-co-caprolactone) (P(LLA-CL)), collagen and chitosan based tubular scaffold with multi-layered symmetrical structure. The multi-layered composite scaffold showed improved mechanical property and biocompatibility, in comparison to the blended scaffold using the same proportion of raw materials. Endothelialization on the multi-layered scaffold was accelerated owing to the bioactive surface made of pure natural materials. hSMCs growth showed the similar results because of its better biocompatibility. Additionally, fibers morphology change, pH value balance and long term mechanical support results showed that the gradient structure effectively improved biodegradability. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Jun 2015 · Colloids and surfaces B: Biointerfaces
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    ABSTRACT: Cornea transplant technology has progressed markedly in recent decades, allowing surgeons to replace diseased corneal endothelium by a thin lamellar structure. A thin, transparent, biocompatible, tissue-engineered substratum with corneal endothelial cells for endothelial keratoplasty is currently of interest. Electrospinning a nanofibrous structure can simulate the extracellular matrix and have beneficial effects for cell culture. Silk fibroin (SF) has good biocompatibility but poor mechanical properties, while poly(l-lactic acid-co-ε-caprolactone) (P(LLA-CL)) has good mechanical properties but poor biocompatibility. Blending SF with P(LLA-CL) can maintain the advantages of both these materials and overcome their disadvantages. Blended electrospun nanofibrous membranes may be suitable for regeneration of the corneal endothelium. The aim of this study was to produce a tissue-engineered construct suitable for endothelial keratoplasty. Five scaffolds containing different SF:P(LLA-CL) blended ratios (100:0, 75:25, 50:50, 25:75, 0:100) were manufactured. A human corneal endothelial (B4G12) cell line was cultured on the membranes. Light transmission, speed of cell adherence, cell viability (live-dead test), cell proliferation (Ki-67, BrdU staining), and cell monolayer formation were detected on membranes with the different blended ratios, and expression of some functional genes was also detected by real-time polymerase chain reaction. Different blended ratios of scaffolds had different light transmittance properties. The 25:75 blended ratio membrane had the best transmittance among these scaffolds. All electrospun nanofibrous membranes showed improved speed of cell adherence when compared with the control group, especially when the P(LLA-CL) ratio increased. The 25:75 blended ratio membranes also had the highest cell proliferation. B4G12 cells could form a monolayer on all scaffolds, and most functional genes were also stably expressed on all scaffolds. Only two genes showed changes in expression. All blended ratios of SF:P(LLA-CL) scaffolds were evaluated and showed good biocompatibility for cell adherence and monolayer formation. Among them, the 25:75 blended ratio SF:P(LLA-CL) scaffold had the best transmittance and the highest cell proliferation. These attributes further the potential application of the SF:P(LLA-CL) scaffold for corneal endothelial transplantation.
    No preview · Article · May 2015 · International Journal of Nanomedicine
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    ABSTRACT: Although the thiol click reaction is an attractive tool for post-polymerization modification of thiolmers, thiol groups are easily oxidized, limiting the potential for covalent immobilization of bioactive molecules. In this study, a series of biodegradable polyurethane elastomers incorporating stable cyclic disulfide groups was developed and characterized. These poly(ester urethane)urea (PEUU-SS) polymers were based on polycaprolactone diol (PCL), oxidized DL-dithiothreitol (O-DTT), lysine diisocyanate (LDI) or butyl diisocyanate (BDI), with chain extension by putrescine. The ratio of O-DTT:PCL was altered to investigate different levels of potential functionalization. PEG acrylate was employed to study the mechanism and availability of both bulk and surface click modification of PEUU-SS polymers. All synthesized PEUU-SS polymers were elastic with breaking strengths of 38-45 MPa, while the PEUU-SS(LDI) polymers were more amorphous, possessing lower moduli and relatively small permanent deformations versus PEUU-SS(BDI) polymers. Variable bulk click modification of PEUU-SS(LDI) polymers was achieved by controlling the amount of reduction reagent, and rapid reaction rates occurred using a one-pot, two-step process. Likewise, surface click reaction could be carried out quickly under mild, aqueous conditions. Furthermore, a maleimide-modified affinity peptide (TPS) was successfully clicked on the surface of an electrospun PEUU-SS(BDI) fibrous sheet, which improved endothelial progenitor cell adhesion versus corresponding unmodified films. The cyclic disulfide containing biodegradable polyurethanes described provide an option for soft tissue regenerative medicine applications where a temporary, elastic scaffold with designed biofunctionality from a relatively simple click chemistry approach is desired.
    No preview · Article · Apr 2015 · Biomacromolecules
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    Xi Chen · Jing Wang · Qingzhu An · Dawei Li · Peixi Liu · Wei Zhu · Xiumei Mo
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    ABSTRACT: Emulsion electrospinning is a convenient and promising method for incorporating proteins and drugs into nanofiber scaffolds. The aim of this study was to fabricate a nanofiber scaffold for anticoagulation and rapid endothelialization. For this purpose, we encapsulated heparin and vascular endothelial growth factor (VEGF) into the core of poly(l-lactic acid-co-ɛ-caprolactone) (P(LLA-CL)) core-shell nanofibers via emulsion electrospinning. The fiber morphology, core-shell structure and hydrophilicity of the nanofiber mats were analyzed by scanning electron microscopy, transmission electron microscopy and water contact angle. The blood compatibility was measured by hemolysis and anticoagulation testing. A CCK-8 assay was performed to study the promotion of endothelial progenitor cell (EPC) growth and was complemented by immunofluorescent staining and SEM. Our study demonstrates that heparin and VEGF can be incorporated into P(LLA-CL) nanofibers via emulsion. The released heparin performed well as an anticoagulant, and the released VEGF promoted EPC growth on the fiber scaffolds. These results imply that electrospun P(LLA-CL) nanofibers containing heparin and VEGF have great potential in the development of vascular grafts in cases where antithrombogenicity and accelerated endothelialization are desirable. Copyright © 2015 Elsevier B.V. All rights reserved.
    Full-text · Article · Feb 2015 · Colloids and surfaces B: Biointerfaces
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    ABSTRACT: In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides and it's denaturation temperature was 44.99°C. It didn't change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4+/CD8+ lymphocytes and the level of IgG or IgM in Sprague-Dawley (SD) rat. The contact angle, tensile strength and weight loss temperature of collagen nanofibers were 21.2°, 6.72±0.44Mpa and 300°C, respectively. The nanofibers could promote the viabilities of human keratinocytes (HaCaTs) and human dermal fibroblasts (HDFs), inducing epidermal differentiation through the gene expression of involucrin, filaggrin and type I transglutaminase (TGase1) of HaCaTs. And they could also accelerate HaCaTs migration with the expression of matrix metalloproteinase (MMP)-9 and transforming growth factor (TGF)-β1. Besides, the nanofibers could upregulate the protien level of Col-I in HDFs both via direct effect and TGF-β1 secreted from HaCaTs, thus facilitating the formation of collagen fibers. Furthermore, the collagen nanofibers stimulated the skin regeneration rapidly and effectively in vivo. These biological effects could be explained as the contributions from the biomimic extracellular cell matrix structure, hydrophilicity and the multiple amino acids of the collagen nanofibers.
    Preview · Article · Jan 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: Particular attention has been given to three-dimensional scaffolds for bone tissue regeneration. In this study, poly(l-lactic acid-co-ε-caprolactone) (P(LLA-CL) nanoyarn scaffold and poly(l-lactic acid-co-caprolactone)/silk fibroin (P(LLA-CL)/SF) nanoyarn scaffold were fabricated by a dynamic liquid support electrospinning system; and then the three-dimensional (3D) nanoyarn scaffolds were prepared by freeze-drying processes. The results indicated the average diameter of P(LLA-CL) and P(LLA-CL)/SF nanoyarns were 29.44 ± 3.47 μm and 11.59 ± 0.46 μm, respectively. The yarn in the nanoyarn scaffold was twisted by many nanofibers as evidenced by scanning electron microscope (SEM) result. These nanoyarn scaffolds were biomineralized by alternatively immersing the nanoyarn scaffolds into phosphoric acid and calcium ion solutions. After biomineralization, the existence of hydroxyapatite (HA) particles on the scaffolds was confirmed using fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. In vitro study of cell proliferation was found to be higher on P(LLA-CL)/SF scaffold as compared to P(LLA-CL) scaffold after culturing for 14 days. H&E staining results showed that cells not only attached to the surface of 3D scaffold but also infiltrated into the scaffold. This study indicated that the electrospun P(LLA-CL)/SF scaffold with nanostructure morphology could improve cell adhesion and proliferation and electrospun P(LLA-CL)/SF scaffold with biomineralization has a potential application for bone tissue engineering.
    Full-text · Article · Dec 2014 · Iranian Polymer Journal
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    ABSTRACT: While surface modification is well suited for imparting biomaterials with specific functionality for favorable cell interactions, the modification of degradable polymers would be expected to provide only temporary benefit. Bulk modification by incorporating pendant reactive groups for subsequent functionalization of biodegradable polymers would provide a more enduring approach. Towards this end, a series of biodegradable poly(ester urethane)urea elastomers with variable amino content (PEUU-NH2 polymers) were developed. Carboxylated phosphorycholine was synthesized and conjugated to the PEUU-NH2 polymers for subsequent bulk functionalization to generate PEUU-PC polymers. Synthesis was verified by proton nuclear magnetic resonance, X-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy. The impact of amine incorporation and phosphorylcholine conjugation was shown on mechanical, thermal and degradation properties. Water absorption increased with increasing amine content, and further with PC conjugation. In wet conditions, tensile strength and initial modulus generally decreased with increasing hydrophilicity, but remained in the range of 5-30 MPa and 10-20 MPa, respectively. PC conjugation was associated with significantly reduced platelet adhesion in blood contact testing and the inhibition of rat vascular smooth muscle cell proliferation. These biodegradable PEUU-PC elastomers offer attractive properties for applications as non-thrombogenic, biodegradable coatings and for blood-contacting scaffold applications. Further, the PEUU-NH2 base polymers offer the potential to have multiple types of biofunctional groups conjugated onto the backbone to address a variety of design objectives.
    Full-text · Article · Nov 2014 · Acta Biomaterialia

Publication Stats

1k Citations
348.94 Total Impact Points

Institutions

  • 2015
    • Shanghai Jiao Tong University
      • Department of Ophthalmology
      Shanghai, Shanghai Shi, China
  • 2007-2015
    • Donghua University
      • • College of Chemistry, Chemical Engineering & Biotechnology
      • • State Key Laboratory for Modification of Chemical Fibers and Polymer Materials
      • • College of Materials Science and Engineering
      • • Institute of Biological Sciences and Biotechnology
      Shanghai, Shanghai Shi, China
  • 2011-2014
    • King Saud University
      • Department of Chemistry
      Ar Riyāḑ, Ar Riyāḑ, Saudi Arabia
  • 2000
    • East China University of Science and Technology
      Shanghai, Shanghai Shi, China