Yonghao Li

Sun Yat-Sen University, Shengcheng, Guangdong, China

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Publications (12)16.38 Total impact

  • Jinglin Zhang · Yonghao Li · Xiujuan Zhao · Xiling Yu · Lin Lu
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    ABSTRACT: Purpose: To compare the clinical features after 20-gauge (20G) versus 23-gauge (23G) pars plana vitrectomy (PPV). Design: This was a prospective observational study. Methods: Patients who underwent 20G or 23G PPV participated in the study. Sutures were used in all patients who had 20G and as necessary in patients who had 23G. All patients were examined with ultrasound biomicroscopy and tonometry for intraocular pressure (IOP) preoperatively and postoperatively at 1 day, 1 week, 1 month, and 6 months. Results: Forty-nine eyes underwent 20G PPV and 97 eyes underwent 23G PPV. Hypotony appeared more frequently in the 23G group (9 patients of 97, 9%) than in the 20G group (1 patient of 49, 2%) 1 day after surgery. Mean IOP was statistically significantly lower in the 23G group 1 day after surgery (P = 0.000). Postoperatively, choroidal detachment (CD) was found in both the 23G group (22/97, 23%) and the 20G group (1/49, 2%). In the 23G group, the mean IOP of eyes with CD was significantly lower than those without CD. There was no statistically significant difference in vitreous incarceration between the 2 groups (P = 0.317). Conclusions: Choroidal detachment and hypotony were common complications in the early stages after 23G PPV. The incidence of postoperative vitreous incarceration was similar in both groups.
    No preview · Article · Dec 2015
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    ABSTRACT: To investigate SHH expression and its effects on retinal Müller cells in diabetic rats and in vitro culture systems under high glucose stress. Diabetic rats were induced by intraperitoneal injection of streptozotocin. Primary rat retinal Müller cells were grown in medium containing 5mM glucose or 35mM glucose with SHH or/and cyclopamine. Retinas and Primary Müller cells expression of SHH pathway components protein and mRNA were determined by Western blot analysis and real-time PCR. The effects of exogenous SHH and its inhibitor cyclopamine on retinal ganglion cells (RGCs) survival after 3-month diabetes were examined by the counting of Brn-3a-labled RGCs. PI3K, ERK1/2 and P38 were detected by Western blot. Both mRNA and protein expression of SHH, SMO, GLI1 and GFAP in the retinas of diabetic rats and high glucose cultured Müller cells increased in a time-dependent manner. Exogenous SHH increased the mRNA and protein expression of SHH, SMO and GLI1 and cyclopamine reversed that effect. Three months after onset of diabetes, administration of SHH inhibited gliosis significantly and promoted RGC survival. Exogenous SHH upregulated the phosphorylation of phosphoinositide 3-kinase (PI3K) and downregulated extracellular signal regulated kinases 1 and 2 (ERK1/2), but did not affect the expression of P38. SHH signaling pathway was upregulated in diabetic rats retina and high glucose cultured Müller cells, and SHH exerted neuroprotective effects on damaged RGCs in a rat diabetes model. The neuroprotective effects of SHH may act indirectly, via Müller cells, through PI3K or ERK1/2 pathways. Copyright © 2015 by Association for Research in Vision and Ophthalmology.
    No preview · Article · Mar 2015 · Investigative ophthalmology & visual science
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    ABSTRACT: Background: Diabetes-related pathogenic factors can cause retinal ganglion cell (RGC) apoptosis, but the specific mechanism is not very clear. The aim of this study is to investigate the correlation between glycogen synthase kinase-3 (GSK-3) activation and retinal neuron apoptosis. Methods: In an in vitro experiment, the number of apoptotic RGC-5 cells differentiated by staurosporine was evaluated via flow cytometry and nuclei staining using Hoechst 33258. GSK-3 phosphorylation and caspase-3 activation in RGC-5 cells after serum deprivation were determined using Western blotting. Mitochondrial membrane potential was detected using the dye 5, 5', 6, 6'tetrachloro-1, 1', 3, 3'-tetrethyl benzimidalyl carbocyanine iodide, and reactive oxygen species (ROS) levels were measured with dihydroethidium. In an in vivo experiment, the number of apoptotic retinal neurons was evaluated via terminal transferase dUTP nick-end labeling (TUNEL), and GSK-3 phosphorylation was determined using Western blotting, in the retinal nerve epithelial tissue of rats in which diabetes was induced by intravenous tail-vein injection of streptozotocin for 4 weeks. Results: The levels of phosphorylated Ser21/9 in GSK-3α/β and p-T308/S473-AKT were lower and the cleaved caspase-3 levels were higher in the serum-deprived model (P < 0.05). Lithium chloride treatment was associated with a slower rate of apoptosis, increased mitochondrial membrane potential, and decreased ROS levels in differentiated RGC-5 cells (P < 0.05). The level of blood glucose and the number of TUNEL-positive cells in the whole-mounted retinas were higher (P < 0.01), and the levels of phosphorylated Ser21/9 in GSK-3α/β and body weight were lower (P < 0.05). However, the thickness of the retinal nerve epithelial layer was not significantly less in diabetic rats compared with control group. Lithium chloride intravitreal injection increased the levels of phosphorylated Ser21/9 in GSK-3α/β and decreased TUNEL-positive cells in the whole-mounted retinas. Conclusion: GSK-3 kinase is closely related to retinal neuron apoptosis, and the application of the GSK-3 inhibitor lithium chloride can reduce retinal neuron apoptosis in early diabetic retinopathy.
    No preview · Article · Oct 2014 · Chinese medical journal
  • Yonghao Li · Jost B Jonas · Lin Lu

    No preview · Article · Jan 2014 · Acta ophthalmologica
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    Jinglin Zhang · Xiujuan Zhao · Yu Cai · Yonghao Li · Xiling Yu · Lin Lu
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    ABSTRACT: Studies have shown that mini-αA can protect retinal pigment epithelium (RPE) cells from apoptosis. However, no in vivo study concerning the anti-apoptotic function of mini-αA has been conducted yet. MTT assay, HE staining and TUNEL assay were used to assess levels of cells, and an animal model was established to examine the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Western blot analysis and RT-qPCR were performed to explore the possible mechanism of mini-αA's protective function against NaIO3-induced RPE cell apoptosis. RESULTS from in vivo and animal experiments showed that mini-αA antagonized NaIO3-induced RPE cell apoptosis. Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy. In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. mini-αA can antagonize RPE cell apoptosis induced by NaIO3. A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.
    Preview · Article · Jan 2014 · International Journal of Molecular Sciences
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    ABSTRACT: Locating the medial cut end of the severed canaliculus is the most difficult aspect of canalicular repair, especially in patients with more medial laceration, severe oedema, persistent errhysis and a narrow canaliculus. Irrigation is a widely used technique to identify the cut end; however, we found that air injected through the intact canaliculus with a straight needle failed to reflux when the common canaliculus or lacrimal sac was not blocked. We describe a simple, safe and efficient air-injection technique to identify the medial cut edge of a lacerated canaliculus. In this method, we initially submersed the medial canthus under normal saline, then injected filtered air through the intact canaliculus using a side port stainless steel probe with a closed round tip. The tip was designed to block the common canaliculus to form a relatively closed system. The efficiency of this novel air-injection technique was equivalent to the traditional technique but does not require the cooperation of the patient to blow air. Using this technique, the medial cut end was successfully identified by locating the air-bubble exit within minutes in 19 cases of mono-canalicular laceration without any complication.
    No preview · Article · Sep 2013 · The British journal of ophthalmology
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    ABSTRACT: : To investigate the characteristics of rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) in a Chinese population by using ultrasound biomicroscope (UBM). : A random prospective study was used to assess 1,389 patients (1,408 eyes) with rhegmatogenous retinal detachment. Comprehensive standardized ophthalmic examinations included intraocular pressure (IOP) by Goldmann applanation tonometer and the entire ocular fundus by Goldmann three-mirror lens, B-type ultrasound, and UBM. : A total of 1,389 patients with rhegmatogenous retinal detachment participated in the study, including 906 men and 483 women. Two hundred and sixty-three eyes (18.79%) had RRDCD and the age of patients ranged from 9 to 82 years (median, 50.8 ± 17.7 years). Patients with high myopia (-6.00 diopters or more) represented 31.94% and more frequently experienced low intraocular pressure and multiple retinal breaks. In 263 RRDCD eyes, the results of UBM showed that ranges of choroidal detachment (CD) were <1 quadrant in 9 (3.42%) eyes, from 1 to 2 quadrants in 37 (14.07%) eyes, from 2 to 3 quadrants in 13 (4.94%) eyes, and from 3 to 4 quadrants in 204 (77.57%) eyes. ultrasound biomicroscope also showed that the angular ranges of CD were between 3.31° and 45.65° (mean, 15.98 ± 9.29°). In addition, UBM showed the mean values of CD angle for the superior (15.42 ± 9.40°), nasal (14.08 ± 8.66°), inferior (15.03 ± 9.05°), and temporal (15.96 ± 9.22°) sides. No statistically significant differences were found among any of these comparisons (F value = 2.011, P > 0.05). : Retinal detachment associated with CD occurs more frequently in older people or in patients with high myopia, who usually present with low intraocular pressure and multiple retinal breaks. UBM examination can reduce false-negative rate of RRDCD, determine CD degree quantitatively, and guide classification and treatment. UBM combined with B ultrasound testing can provide a precise diagnosis of rhegmatogenous retinal detachment degree and may have important clinical significance for preoperative diagnosis of anterior proliferative vitreoretinopathy and determining operative strategy and postoperative prognosis.
    No preview · Article · May 2012 · Retina (Philadelphia, Pa.)
  • Lingyan Chen · Lin Lü · Ping Zhang · Yonghao Li · Jianxian Lin
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    ABSTRACT: Vitreous amyloidosis is a rare condition that mainly occurs in Familial Amyloidotic Polyneuropathy (FAP). In some cases, it may be the only symptom without systemic disorders. One case of familial vitreous amyloidosis was reported here, with white, wispy opacities in vitreous cavity in both eyes. Pars plana vitrectomy and histopathological examination of the vitreous specimens were performed. The vitreous specimens showed typical microscopic features of amyloidosis with Congo red stain and non-branching fibrils on a transmission electron microscope. Transthyretin (TTR) gene was amplified with DNA isolated from the peripheral blood cells. Bi-directional sequencing of exon 3 showed a single base-pair substitution, which results in an amino acid substitution at position83, glycine to arginine (TTR Arg-83). TTR Arg-83 may be a new pathologic mutation in vitreous amyloidosis.
    No preview · Article · Apr 2008 · Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
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    ABSTRACT: To investigate the levels of stromal cell-derived factor-1 (SDF-1) and vascular endothelial growth factor (VEGF) in the vitreous of patients with proliferative diabetic retinopathy. The levels of SDF-1 and VEGF in the vitreous of 30 eyes of 30 patients with proliferative diabetic retinopathy (PDR) and 12 eyes of 12 patients with idiopathic macular hole (MH) were measured by enzyme-linked immunosorbent assay. Vitreous fluid samples were obtained by vitrectomy. The vitreous concentration of VEGF was significantly higher in eyes with PDR (2,865.87 +/- 387.85 pg/ml) than in eyes with idiopathic macular hole (142.42 +/- 21.03 pg/ml, P < 0.0001). The vitreous level of SDF-1 was also significantly higher in eyes with PDR (298.40 +/- 24.57 pg/ml) than in eyes with idiopathic macular hole (86.91 +/- 15.89 pg/ml, P < 0.0001). The vitreous concentration of SDF-1 correlated significantly with that of VEGF in eyes with PDR ([correlation coefficient] r = 0.62, P < 0.001). Vitreous levels of both SDF-1 and VEGF in patients with PDR are significantly higher than those of nondiabetic patients. SDF-1 may be correlated with VEGF in angiogenesis in PDR.
    No preview · Article · Mar 2008 · Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
  • Jinglin Zhang · Lin Lu · Xiulan Lu · Yonghao Li
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    ABSTRACT: To evaluate the nibble technology in the therapy of diabetic retinopathy. Eighty-three patients (ninety-two eyes) with proliferative diabetic retinopathy were treated in our department. All the patients received a standard pars plana vitrectomy. Fifty-two of them (fifty-eight eyes) were treated with nibble technology, while the other thirty-one (thirty-four eyes), who were treated with common procedure to tear the proliferative membrane, were served as a control group. The operative complication of iatrogenic retinal tears was observed in 2 eyes of the test group and 7 eyes of the control group (P < 0.05). Another operative complication of hemorrhage was observed in 4 eyes of the test group and 15 eyes of the control group (P < 0.01). There were 6 eyes of the test group and 5 eyes of the control group presented with grade-III postoperative responds (P > 0.01). The nibble technology is safe in the therapy of proliferative diabetic retinopathy and could reduce the complications during or after the operation.
    No preview · Article · Oct 2006 · Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
  • Xiulan Lu · Lin Lu · Yonghao Li · Jinglin Zhang · Shiyi Li
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    ABSTRACT: To obtain the gene encoding retinal specific Rho promoter therefore to prepare for exogenous gene transcription and expression in retina. Extract the genomic DNA of a BLAB/c mice . The Rho promoter DNA were then amplified by PCR, the product of which was subcloned into PcDNA3.1 +vector. After identification by restriction enzymes, the recombinant PcDNA3.1+vectors were subjected to sequence analysis. The fragments of the promoter as amplified by PCR were of predicted length. Digestion with HindIII and NheI proved correct insertion of the target fragments with expected length into the recombinant PcDNA3.1+vectors. As indicated by homology analysis the Rho promoter was highly conservative, and the Rho promoter we got had hole homology as reported by Boulanger A etc. Retinal specific Rho promoter has been successfully cloned, thus making possible the subsequent research as the mechanism and gene therapy of retina.
    No preview · Article · Jul 2005 · Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
  • Lin Lu · Yonghao Li · Changxian Yi · Mei Li · Xiulan Lu · Jinglin Zhang
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    ABSTRACT: To document the anatomic and functional improvement of six patients with branch retinal vein occlusion (BRVO) following successful arteriovenous adventitial sheathotomy (AAS). Retrospective study of 6 patients (6 eyes) with BRVO treated with AAS. All patients were not eligible for laser photocoagulation and had both macular edema and intraretinal hemorrhage. The visual acuity was in the range of 0.4 to 0.02. All patients underwent pars plana vitrectomy and AAS. The clinical improvement was determined by fundus photograph, fluorescein angiography (FAG), optical coherence tomography (OCT) and multifocal electroretinography (ERG). All patients were followed postoperatively for an average of 20 months ranging from 12 to 24 months. Sheathotomy and decompression of the arteriole/venule (A/V) crossing were achieved in all 6 patients. 5 patients have improved their best-corrected visual acuity 4 lines or more. The best one could reach to 1.0. One month after the operation, fundus photograph and FAG demonstrated the resolution of intraretinal hemorrhage, reduction of non-perfusion area and apparent resolution of retinal venous dilation and tortuosity. OCT confirmed remarkable reduction of retinal thickness. The microcysts at the fovea diminished. Multifocal ERG showed the recovery of the central peak at the macular and the peripheral response density. However, capillary nonperfusion area and microaneurysm were found out by FAG in four patients at the points distal to the sheathotomy three months after the operation. Anatomic and functional improvement of retina can be achieved in patients with BRVO through AAS. However, the capillary nonperfusion and microaneurysm may follow this surgical procedure in some cases that need further treatment with laser photocoagulation. The better visual improvement may be expected in the patients with earlier surgical intervention.
    No preview · Article · Apr 2003 · Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu

Publication Stats

22 Citations
16.38 Total Impact Points


  • 2012-2015
    • Sun Yat-Sen University
      • State Key Laboratory of Oncology
      Shengcheng, Guangdong, China
  • 2005
    • Guangzhou First People's Hospital
      Shengcheng, Guangdong, China