Kui Jiao

Qingdao University of Science and Technology, Tsingtao, Shandong Sheng, China

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Publications (258)644.03 Total impact

  • Xinxing Wang · Wenqing Ma · Tong Ge · Tao Yang · Kui Jiao
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    ABSTRACT: Polymer inorganic nanosheet composites hold great promise in electrochemical sensing applications by improving the electrochemical performance and increasing the surface area. In this work, a reductively treated thin layer molybdenum disulfide nanosheet-poly(xanthurenic acid) (rTLMoS2-PXa) composite have been facilely prepared through a one-step electrosynthesis procedure. The TLMoS2 was used as building block for the construction of the composite with the PXa coated on it. The composite modified electrode possesses improved electron transfer capability and exhibits good electrochemical sensing performance towards several heterocyclic and aromatic ring compounds (2′-deoxyguanosine-5′-triphosphate trisodium salt, dGTP, bisphenol A, BPA and 2,4,6-trinitrotoluene, TNT), which have good affinity to the electroactive PXa and never or rarely been analyzed by MoS2-based sensing platform. This research provides a new electrochemical sensing platform for simple and sensitive detection of dGTP, BPA and TNT, and further extends the application of MoS2 in the field of electrochemical sensing.
    No preview · Article · Jan 2016
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    ABSTRACT: The low electronic conductivity limits the application of molybdenum disulfide (MoS2) in electrochemical sensing field, let alone the label-free and self-signal amplification DNA sensing. Aiming at this problem, a novel polyaniline-molybdenum disulfide (PANI-MoS2) nanocomposite through a facile oxidation polymerization aniline monomer on the thin-layer MoS2 matrix is prepared, which is preobtained via a simple ultrasonic exfoliation method. It is generally appreciated that conducting polymers, e.g., polyaniline (PANI), can work as a direct transducer based on themselves-signal without any label or indicator. Moreover, MoS2 not only serves as an excellent conductive skeleton provided a high electrolytic accessible surface area for redox-active PANI, but also supports a direct path for electron transfer. Therefore, the hybrid of PANI and MoS2 exhibits superior electrochemical performance to pure PANI and MoS2, and can be considered as a superior candidate for direct and label-free electrochemical DNA sensing. A series of experiments are performed to investigate the effect of synthetic conditions (component, dosage of MoS2, and reaction time) on probe DNA (pDNA) immobilization and hybridization adopting the PANI self-signal as the measure signal. The results show that the dosage of MoS2 has a more influence on pDNA immobilization and hybridization.
    No preview · Article · Jan 2016 · Advanced Materials Interfaces
  • Le Meng · Huaiyin Chen · Tong Ge · Tao Yang · Kui Jiao
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    ABSTRACT: A variety of sulfonated polyaniline-graphene oxide (SPAN-GNO) nanocomposites based on GNO, aniline (ANI) and m-aminobenzenesulfonic acid (ABSA) are prepared via changing the mole ratio of ANI to ABSA for the comparison of DNA sensing behavior. Self-signals of SPAN-GNO are employed for estimating the effect of preparation conditions [component, monomer composition (mole ratio of ANI to ABSA), and reaction time] on DNA immobilization and hybridization detection. Then, we find herein that the mole ratio of ANI to ABSA plays a lead role over other factors on hybridization efficiency. Meanwhile, the parallel experiments using methylene blue as the classic indicator verifies this conclusion. The results show that, by comparison with other mole ratio SPAN-GNO nanocomposites-modified electrodes, the mole ratio (2:3) exhibits the widest dynamic detection range from 1.0 × 10−14 to 1.0 × 10−6 M, as well as the lowest detection limit (3.06 × 10−15 M). © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016
    No preview · Article · Jan 2016 · Journal of Polymer Science Part A Polymer Chemistry
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    ABSTRACT: Since controlling substrates can program the electrochemical properties, many researchers have devoted to investigate the corresponding mechanism. However, up to now, the effects of substrate (such as morphology, size, material composition) on the performance of electrochemical sensors have been mainly focused on metal platform, while the investigation about metal oxide substrate has not been reported in detail. Zinc oxide (ZnO) is a technologically important semiconducting material with large band gap energy between 3.2 and 3.4 eV at room temperature which has attracted widespread attentions. In previous reports, the nanostructured ZnO was randomly formed on graphene layers. The control of morphology or size of nanostructures is a prerequisite for the nanostructure for fabricating various types of nanocomposites. Here, we used graphene oxide (GNO) as the supporting material for constructing a series of synchronously electrochemically reduced graphene oxide and zinc oxide composites (rGNO-ZnO) with different morphologies for comparing their morphology-dependent electrochemical sensing behaviors. The experimental parameters, such as the electrodeposition potential, time, and concentration of electrolyte, would influence the composite morphology and further bring different electrochemical sensing ability. Among them, rGNO-ZnO with nanowalls morphology (noted GZNWs), obtained from the conditions of 0.1 M Zn(NO3)2 electrolyte, -1.0 V electrodeposition potential and 30 min electrodeposition time, presented an optimal ability for DNA detection and 2, 4, 6-Trinitrotoluene (TNT) electrocatalysis. The fine nanowall structure may be able to provide more accessible sites, and the synergistic effect between rGNO and ZnO may enlarge the electrochemical activity. The electrochemical DNA sensor based on GZNWs exhibited the steepest slope with the detection range, as well as the highest sensitivity when compared with other electrodeposition potentials. In order to further explore the other electrochemical performances (for instance the electrocatalysis), the GZNWs was used to detect the nitroaromatic compound (such as 2, 4, 6-Trinitrotoluene, TNT). It had reached the aim of improving the sensitivity of their detection.
    No preview · Article · Oct 2015
  • Huai-Yin Chen · Jin Wang · Le Meng · Tao Yang · Kui Jiao
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    ABSTRACT: In this study, we synthesized molybdenum disulfide/polyaniline (MoS2/PANI) nanocomposite via in situ polymerization of aniline in the presence of thin-layered MoS2. The as-prepared MoS2/PANI nanocomposite obtained an improved electrochemical performance due to the physisorption interaction between aromatic aniline and the basal plane of MoS2. Furthermore, we constructed a new kind of electrochemical sensor based on MoS2/PANI nanocomposite for the detection of chloramphenicol, which showed an excellent performance. The sensor has a high sensitivity and wide detection range from 1 × 10−7 mol/L to 1 × 10−4 mol/L, with a low detection limit of 6.9 × 10−8 mol/L.
    No preview · Article · Sep 2015 · Chinese Chemical Letters
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    ABSTRACT: Thin-layered molybdenum disulfide (MoS2) was intercalated, via ultrasonic exfoliation, into self-doped polyaniline (SPAN). This material, when placed on a glassy carbon electrode (GCE), exhibits excellent electrical conductivity and synergistic catalytic activity with respect to the detection of bisphenol A (BPA). The electrochemical response of the modified GCE to BPA was investigated by cyclic voltammetry and differential pulse voltammetry. Under optimal conditions, the oxidation peak current (measured best at 446 mV vs. SCE) is related to the concentration of BPA in the range from 1.0 nM to 1.0 μM, and the detection limit is 0.6 nM. Graphical Abstract Thin-layered molybdenum disulfide (MoS2) was intercalated into self-doped polyaniline (SPAN) via ultrasonic exfoliation. The special conjugated structure and functional groups of MoS2-SPAN composite help to adsorb BPA easily. MoS2-SPAN has a synergistic effect for catalyzing the oxidation of BPA. The BPA electrochemical sensor based on MoS2-SPAN has a high sensitivity and low detection limit.
    No preview · Article · Aug 2015 · Microchimica Acta
  • Tao Yang · Huaiyin Chen · Tong Ge · Jin Wang · Weihua Li · Kui Jiao
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    ABSTRACT: The nanocomposite of molybdenum disulfide (MoS2) and polyaniline (PANI) was prepared through in situ polymerization of aniline on the surface and interlayer of thin-layered MoS2. Owing to the physisorption of aromatic aniline onto the basal plane of MoS2, the electrochemical properties of MoS2/PANI nanocomposite were improved. And a novel electrochemical sensor based on MoS2/PANI nanocomposite was used to determine chloramphenicol by differential pulse voltammetry, exhibiting excellent performance. The detection range was from 1×10-7 mol L-1 to 1×10-4 mol L-1, with a high sensitivity and a low detection limit of 6.9×10-8 mol L-1. In addition, this sensor can be used for the determination of chloramphenicol in real samples.
    No preview · Article · Aug 2015 · Talanta
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    ABSTRACT: Until now, researches on the preparation of MoS2-based polymer nanocomposites with the electropolymerization method are scarce. Herein, for the first time, a poly (xanthurenic acid, Xa) film based on thin-layer MoS2 support was electrochemically prepared to form a highly electroactive biosensing platform. The thin-layer MoS2 were obtained with a simple ultrasonic method from bulk MoS2. The physical adsorption between MoS2 and aromatic Xa improved the electropolymerization efficiency, accompanied with an increased electrochemical response of PXa. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were utilized to character the morphology and certify the electrochemical properties of the prepared interface. Compared with sole PXa or MoS2 modified electrode, the PXa-MoS2 hybrid interface exhibited the good electrocatalytic activity and the prominent synergistic effect on guanine and adenine. PXa-MoS2 nanocomposite owns the negative charge and specific structure, which obviously prompt the adsorption of the positively charged guanine and adenine. Moreover, this nanocomposite is a promising candidate in electrochemical sensing and other electrocatalytic applications.
    No preview · Article · Jun 2015 · Journal of Materials Chemistry B
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    ABSTRACT: Until now, morphology effects of 2-dimensional or 3-dimensional graphene nanocomposites and the effect of material composition on the biosensors have been rarely reported. In this paper, the various nanocomposites based on graphene oxide and self-doped polyaniline nanofibres for studying the effect of morphology and material composition on DNA sensitivity were directly reported. The isolation and dispersion of graphene oxide were realized via intercalated self-doped polyaniline and ultrasonication, where the ultrasonication prompts the aggregates of graphite oxide to break up and self-doped polyaniline to diffuse into the stacked graphene oxide. Significant electrochemical enhancement has been observed due to the existence of self-doped polyaniline, which bridges the defects for electron transfer and, in the mean time, increases the basal spacing between graphene oxide sheets. Different morphologies can result in different ssDNA surface density, which can further influence the hybridization efficiency. Compared with 2-dimensional graphene oxide, self-doped polyaniline and other morphologies of nanocomposites, 3-dimensional graphene oxide-self-doped polyaniline nanowalls exhibited the highest surface density and hybridization efficiency. Furthermore, the fabricated biosensors presented the broad detection range with the low detection limit due to the specific surface area, a large number of electroactive species, and open accessible space supported by nanowalls. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · May 2015 · Colloids and surfaces B: Biointerfaces
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    ABSTRACT: Recently, functional composites based on chemically modified graphenes (CMGs) and nanostructured conducting polymers have attracted wide interest in electrochemical biosensing field. However, comprehensive studies about the effect of various CMGs on the electrochemical properties and biosensing performance of the composites are scarce. Herein, for the first time, we fabricated and deeply evaluated three composites composed of CMGs and sulfonic acid-doped polyaniline nanofiber (namely CMG-SPAN composites). The CMGs (involving the unreduced form and reduced forms prepared by different reduction routes) were chosen to show the effect of reduction and different preparation routes on the morphologies, electrochemical properties and DNA biosensing performances of the composites. Notably, the self-redox signals of SPAN in these composites were significantly enhanced and have been adopted for rapid, direct and label-free DNA detection. Moreover, a preliminary study toward capacitive characteristics of thermally reduced graphene oxide-SPAN composite has been conducted at the end of this paper, owing to the potential benefits of the composite in supercapacitor which were surprisingly observed in this research. The findings in this work will provide useful guides for a better understanding of the interaction between CMG and SPAN, and for future development of high-performance functional materials for electrochemical sensors/biosensors and supercapacitors.
    No preview · Article · Apr 2015 · The Journal of Physical Chemistry C
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    ABSTRACT: In this paper, a novel molybdenum disulfide (MoS2) intercalated by self-doped polyaniline (SPAN) via ultrasonic exfoliating method was prepared to show outstanding conductivity and synergistic electrocatalytic activity using chloramphenicol (CAP) as a case. In the ultrasonic process, due to the strong π-π(⁎) stacking interaction and electrostatic repulsion, the negatively charged SPAN served as an intercalator to result in few-layers MoS2 nanosheets, which were exfoliated from bulk MoS2. This nanocomposite was characterized by scanning electron microscopy, transmission electron microscopy and differential pulse voltammetry. The obtained nanocomposite owns large conjugated structure and rich negative charge, which can improve the adsorption of conjugate structured CAP with the detection range from 0.1 to 1000μmolL(-1). The results also showed that the electrocatalytic responses were further affected by the mass ratio of SPAN-MoS2 and the ultrasonication time. Our electrocatalytic platform could be further applied in the adsorption and detection of other positively charged biomolecules or aromatic molecules.
    No preview · Article · Jan 2015 · Talanta
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    ABSTRACT: Recently, easy, green and low-cost liquild exfoliation of bulks materials to obtain thin-layered nanostructure significantly emerged. In this work, thin-layered molybdenum disulfide (MoS2) nanosheets were fabricated through intercalation of self-doped polyaniline (SPAN) to layer space of bulk MoS2 by ultrasonic exfoliating method to effective prevent re-aggregation of MoS2 nanosheets. The obtained hybrid showed specific surface area, a large number of electroactive species, and open accessible space, accompanied with rich negative charged and special conjugated structure, which was applied to adopt positively charged guanine and adenine, based on their strong π-π* interactions and electrostatic adsorption. And SPAN-MoS2 interface exhibited the synergistic effect and good electrocatalytic activity compared with the sole SPAN or MoS2 modified electrode.
    No preview · Article · Jan 2015 · ACS Applied Materials & Interfaces
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    ABSTRACT: One-step co-electrodeposition was applied to prepare graphene–zinc oxide nanowalls (GZNWs) composite, where graphene oxide was electrochemically reduced and zinc oxide was electrodeposited simultaneously. The morphologies and the electrochemical properties of GZNWs were obviously influenced by the electrodeposition time. The contrast experiments illuminate that GZNWs presented superior electrochemical activity.
    No preview · Article · Jan 2015 · Materials Letters
  • Tao Yang · Le Meng · Jinlong Zhao · Xinxing Wang · Kui Jiao
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    ABSTRACT: DNA detection sensitivity can be improved by carefully controlling the texture of the sensor substrate, which was normally investigated on metal or metal oxide nanostructured platform. Morphology effects on the biofunctionalization of polymer micro/nanoelectrodes have not been investigated in detail. To extend this topic, we used graphene oxide (GNO) as the supporting material to prepare graphene-based polyaniline nanocomposites with different morphologies as a model for comparing their DNA sensing behaviors. Owing to GNO serving as an excellent support or template for nucleation and growth of polyaniline (PANI), PANI nanostructures grown on GNO substrate were successfully obtained. However, if GNO supporting was absent, the obtained PANI nanowires showed a connected network. Furthermore, adjustment of reaction time can be used for dominating the topographies of PANI-GNO nanocomposites, meaning that different reaction times resulted in various formations of PANI-GNO nanocomposites, including small horns (5 and 12 h), vertical arrays (18 h), and nanotips (24 h). The next-step electrochemical data showed that the DNA electrochemical sensors constructed on the different morphologies possessed different ssDNA surface coverage and hybridization efficiency. Compared with other morphologies of PANI-GNO nanocomposite (5, 12, and 24 h), vertical arrays (18 h) exhibited the highest sensitivity (2.08 × 10(-16) M, 2 orders of magnitude lower than others). It is can be concluded that this nanocomposite with higher surface area and more accessible space can provide an optimal balance for DNA immobilization and DNA hybridization detection.
    No preview · Article · Oct 2014 · ACS Applied Materials & Interfaces
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    ABSTRACT: A label-free and ultrasensitive electrochemical DNA biosensor, based on thin-layer molybdenum disulfide (MoS2) nanosheets sensing platform and differential pulse voltammetry detection, is constructed in this paper. The thin-layer MoS2 nanosheets were prepared via a simple ultrasound exfoliation method from bulk MoS2, which is simpler and no distortion compared with mechanical cleavage and lithium intercalation. Most importantly, this procedure allows the formation of MoS2 with enhanced electrochemical activity. Based on the high electrochemical activity and different affinity toward ssDNA versus dsDNA of the thin-layer MoS2 nanosheets sensing platform, the tlh gene sequence assay can be performed label-freely from 1.0×10(-16)M to 1.0×10(-10)M with a detection limit of 1.9×10(-17)M. Without labeling and the use of amplifiers, the detection method described here not only expands the application of MoS2, but also offers a viable alternative for DNA analysis, which has the priority in sensitivity, simplicity, and costs. Moreover, the proposed sensing platform has good electrocatalytic activity, and can be extended to detect more targets, such as guanine and adenine, which further expands the application of MoS2.
    No preview · Article · Sep 2014 · Biosensors & Bioelectronics
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    ABSTRACT: In order to achieve the large direct electrochemical signals of guanine and adenine, an urgent request to explore novel electrode materials and interfaces has been put forward. In this paper, a poly(xanthurenic acid, Xa)-reduced graphene oxide (PXa-ERGNO) interface, which has rich negatively charged active sites and accelerated electron transfer ability, was fabricated for monitoring the positively charged guanine and adenine. Scanning electron microscopy, Fourier transform infrared spectroscopy, Raman spectra, X-ray photoelectron spectroscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry were adopted to characterize the morphology and prove the electrochemical properties of the prepared interface. The PXa-ERGNO interface with rich negative charge and large electrode surface area was an excellent sensing platform to prompt the adsorption of the positively charged guanine and adenine via strong π-π* interaction or electrostatic adsorption. The PXa-ERGNO interface exhibited prominent synergistic effect and good electrocatalytic activity for sensitive determination of guanine and adenine compared with sole PXa or ERGNO modified electrode. The sensing platform we built could be further applied in the adsorption and detection of other positively charged biomolecules or aromatic molecules.
    No preview · Article · Jul 2014 · ACS Applied Materials & Interfaces
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    ABSTRACT: A novel one-step electrochemical synthesis via a pulse potentiostatic method (PPM) was adopted to prepare a nanocomposite of poly(xanthurenic acid, Xa)–electrochemically reduced graphene oxide (PXa–ERGNO), which was applied for simultaneous detection of guanine and adenine. In the synthesis process, the graphene oxide (GNO) could be electrochemically reduced to reduced graphene oxide in the cathodic potential section; meanwhile, Xa (an unconventional and low toxicity biomonomer) could be electropolymerized in the anodic potential section. The optimization of fabrication was based on the electrooxidation signals of DNA bases. Since the negative charge and specific structure of the nanocomposite can prompt the adsorption of the electropositive guanine and adenine via strong π–π* interactions or electrostatic adsorption, the resulting nanocomposite shows high electrocatalytic ability for the detection of guanine and adenine.
    No preview · Article · Mar 2014
  • Tao Yang · Qian Guan · Le Meng · Ruirui Yang · Qianhe Li · Kui Jiao
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    ABSTRACT: In this paper, we report a simple and low-cost method to prepare large-area, wavy graphene oxide (GNO) nanowalls intercalated by sulfonated polyaniline (SPAN). Through ultrasonication of a mixed dispersion of graphite oxide (GO) and SPAN, the negatively charged SPAN continuously diffused and was adsorbed and intercalated into the simultaneously resulting GNO layers to form a homogenous and three-dimensional interconnected nanowall structure. This unique morphology has a large specific surface area and improves the electrochemical response of [Fe(CN)6]3−/4−, which has been widely adopted to monitor the immobilization and hybridization of DNA. The accessible space, large specific surface area and richly conjugated structures were beneficial to efficiently immobilize a probe DNA via π-π* interactions between the conjugated interface and the DNA bases, which also ensured a highly sensitive and freely switchable impedimetric DNA detection due to a hybridization that induces the dsDNA to be released from the conjugated surface.
    No preview · Article · Oct 2013 · RSC Advances
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    ABSTRACT: In this paper, a type of direct DNA impedance detection using the self-redox signal change of sulfonated polyaniline (SPAN) enhanced by graphene oxide (GNO) was reported, here SPAN is a copolymer obtained from aniline and m-aminobenzenesulfonic acid. The resulting nanocomposite was characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The π-π planar structure of GNO and the carboxyl groups on the surface of GNO ensured it could act as an excellent substrate for adsorption and polymerization of aniline monomer. Because of the existence of GNO, the electrochemical activities of SPAN were enhanced obviously. Owing to abundant sulfonic acid groups, the resulting nanocomposite showed obvious self-redox signal even at physiological pH, which is beneficial for biosensing field. DNA probes with amine groups could be covalently attached to the modified electrode surface through the acyl chloride cross-linking reaction of sulfonic groups and amines. When the flexible probe DNA was successfully grafted, the electrode was coated and electron transfer between electrode and buffer was restrained. Thus, the inner impedance value of SPAN (rather than using outer classic EIS probe, [Fe(CN)6]3-/4-) increased significantly. After hybridization, the rigid helix opened the electron channel, which induced impedance value decreased dramatically. As an initial application of this system, the PML/RARA fusion gene sequence formed from promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARA) was successfully detected.
    No preview · Article · Oct 2013 · ACS Applied Materials & Interfaces
  • Meng Du · QianQian Kong · Tao Yang · Kui Jiao
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    ABSTRACT: Herein, we present the electrochemical co-deposition of Al3+/graphene composites directly from an aqueous mixture containing graphene oxide (GO) and Al3+. The obtained Al3+/graphene composites with good electrochemical activity were regarded as an appropriate immobilization platform for double-stranded DNA (dsDNA). The nontoxic redox probe xanthurenic acid (XA) was successfully applied to recognize single-stranded DNA and dsDNA. We illustrated that the scission of dsDNA caused by GO combining with some metal ions could be detected by monitoring the electrochemical signals of XA.
    No preview · Article · Sep 2013 · Science China-Chemistry

Publication Stats

4k Citations
644.03 Total Impact Points


  • 2003-2016
    • Qingdao University of Science and Technology
      Tsingtao, Shandong Sheng, China
  • 2010
    • Ocean University of China
      • College of Chemistry and Chemical Engineering
      Tsingtao, Shandong Sheng, China
  • 2009
    • Zhangzhou Normal University
      Lunki, Fujian, China
  • 1999
    • Nanjing University
      • Department of Chemistry
      Nan-ching, Jiangsu Sheng, China