G I Abelev

Russian Academy of Sciences, Moskva, Moscow, Russia

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Publications (114)222.89 Total impact

  • G.I. Abelev · E.R. Karamova
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    ABSTRACT: Discontinuous electrophoresis on cellulose acetate membranes with the use of 0.06 M Tris-HCl (pH 6.7) as the leading electrolyte and 0.012 M Tris-beta-alanine (pH 8.6) as the terminating one results in concentration of the proteins present in the system on the Cl-/beta-alanine- boundary. If the antigen solution is placed in a "pocket" ahead of the moving boundary, a counterflow to the cathode arises due to electroendosmosis. At constant voltage the migration rate of the boundary drops and that of electroendosmosis does not change until they become equal. In such a stationary position, the antigen-containing solution is passing through the Cl-/beta-alanine- boundary to the cathode, while all the proteins are completely "absorbed" on the boundary as highly concentrated bands. Addition of ampholytes to the antigen solution contributes to the isotachophoretic separation of a protein mixture on the strip. The concentrated and separated antigens can be revealed by immunofixation, immunodiffusion, or crossed immunoelectrophoresis in gel. The technique is approximately 100 times more sensitive compared to the usual immunodiffusion and immunoelectrophoresis methods on cellulose acetate membranes, and is applicable to the detection of trace amounts of antigens in the urine, liquor, amniotic fluid, tears, and other biological fluids with low protein contents.
    No preview · Article · Dec 1984 · Analytical Biochemistry
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    ABSTRACT: The localization of alpha-fetoprotein (AFP) and of antigens of liver-cell plasma membrane (Ag I, Ag II and Ag III) was studied in adult, postnatal and regenerating (CCl4 or paracetamol-treated mouse liver). Ag I, Ag II and Ag III were solubilized by Triton X-100 from ghosts of the mouse liver cells. The purification of antigens was performed by gel filtration on Sephadex G-200 or Ultrogel ACA-54 with subsequent treatment of antigenic fractions eluted from the column with 5% HClO4. Ag I and Ag II are common for liver and some other mouse organs, but Ag III is strictly specific for liver. The Ag I is predominantly found in the region of the bile capillaries. Ag II and Ag III are mainly present on membrane adjoining blood sinusoids. The distribution of these three antigens in newborn mouse liver is quite different from that in adult liver and reflects the specific arrangement of hepatocytes at different stages of development - the acinar structure in newborn liver and the plate structure in the adult organ. During regeneration as well as during postnatal development, AFP has been found in areas of marked tissue rearrangement. In both cases, these areas lose the plasma membrane antigens, especially Ag I - the antigen of bile capillary. The reappearance of Ag I on the surface of liver cells coincides with cessation of AFP synthesis and establishment of definitive plate structure. The possible role of liver plate structure in the regulation of AFP synthesis is discussed.
    No preview · Article · Jul 1983 · International Journal of Cancer
  • T L Eraĭzer · G I Abelev
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    ABSTRACT: Production of alpha-fetoprotein (AFP) was determined in single cells of hepatoma McA-RH7777 and in the clones of their progeny. To elucidate the heritability of this trait in a series of cell generations, a variety of local hemolysis in gel was devised. According to the method the cells and red cells conjugated with protein A were placed on the polylysine covered surface and layered with agarose gel containing antibodies. AFP production by single cells was determined from the formation of plaques--areas of red cell hemolysis. The cells forming the plaques (+AFP) and not forming them (-AFP) were distinguished and their reproduction was followed up. After 7-14 days the cells were fixed and stained by the immunoperoxidase technique with antibodies to AFP. High efficacy of the cloning has been demonstrated for both +AFP- and -AFP-cells (69 and 71%). Negative cells preserved their phenotype more frequently, producing homogenous negative clones, whereas +AFP cells gave "negative" clones in 1/3 of the cases. Both cells gave mixed clones in a small percentage of the cases. At present the AFP trait in these cells is being studied by recloning.
    No preview · Article · Nov 1982 · Biulleten' eksperimental'noĭ biologii i meditsiny

  • No preview · Article · Jul 1982 · Bulletin of Experimental Biology and Medicine
  • G I Abelev · D A El'gort · T L Eraĭzer
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    ABSTRACT: Clonal cell lines were isolated from rat hepatoma McA-RH7777 to study expression of alpha-fetoprotein (AFP) in these clones. AFP contained by the cells was determined by immunofluorescent and immunoperoxidase staining, while AFP secreted into the medium by aggregate-hemagglutination and immunoisotachophoresis. The existence of the clones that drastically differ in the intensity of AFP synthesis was demonstrated, with these clones being implicated in the monitoring of AFP synthesis. The differences between the clones gradually diminished in the course of long-term cultivation. It was also shown that the clones under consideration secreted, apart from AFP, at least 8 proteins of adult rat serum. One of the proteins was identified as transferrin. No albumin was detected in the cultural media tested. The level of AFP production did not correlate with the synthesis of other serum proteins and thus seems likely to be controlled by an independent mechanism.
    No preview · Article · Oct 1981 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • G. I. Abelev · D. A. Él'gort · T. L. Éraizer

    No preview · Article · Sep 1981 · Bulletin of Experimental Biology and Medicine
  • G.I. Abelev · D.A. El'gort · T.L. Eraǐzer
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    ABSTRACT: Clonal cell lines were isolated from rat hepatoma McA-RH7777 to study expression of alpha-fetoprotein (AFP) in these clones. AFP contained by the cells was determined by immunofluorescent and immunoperoxidase staining, while AFP secreted into the medium by aggregate-hemagglutination and immunoisotachophoresis. The existence of the clones that drastically differ in the intensity of AFP synthesis was demonstrated, with these clones being implicated in the monitoring of AFP synthesis. The differences between the clones gradually diminished in the course of long-term cultivation. It was also shown that the clones under consideration secreted, apart from AFP, at least 8 proteins of adult rat serum. One of the proteins was identified as transferrin. No albumin was detected in the cultural media tested. The level of AFP production did not correlate with the synthesis of other serum proteins and thus seems likely to be controlled by an independent mechanism.
    No preview · Article · Sep 1981
  • G I Abelev · E R Karamova
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    ABSTRACT: A variant of counter-flow isotachophoresis of proteins on cellulose acetate membranes is proposed. The liquid counter-flow is created by electroendosmosis in the membrane. Proteins are concentrated at the Kolrausch boundary during isotachophoresis in the presence of ampholytes. The method permits one to make microanalysis of proteinic mixtures in diluted solutions, and it can be used in combination with immunodiffusion and immunoelectrophoretic methods of antigenic protein detection.
    No preview · Article · Oct 1979 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • A A Sokolenko · G I Abelev
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    ABSTRACT: Two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "Cellogel" has been developed. The method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. The procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitates with antiglobulin antibodies, the complement or their combination. An increase in the method sensitivity up to 30-60 ng/ml in the visible zone of the reaction is achieved by simultaneous reduction in the antibody concentration and the growth of the absolute quantity of the antigen, subjected to electrophoresis, up to 50-100 microliters. The method has been applied to human alpha-fetoprotein.
    No preview · Article · Oct 1979 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • A. A. Sokolenko · G. I. Abelev
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    ABSTRACT: The method of two-stage rocket electrophoresis on gelatinized cellulose acetate film (Cellogel) was developed. The method is based on electroimmunodiffusion detection of antigen on cellulose acetate films containing monospecific antiserum of a test system, followed by detection of precipitation bands with the appearance of stained “rockets” if the reaction takes place in the visible zone, or with further treatment of cellulose acetate strips containing invisible precipitates with antiglobulin antibodies, complement, or a combination of both. The sensitivity of the method is increased to 30–60 ng/ml in the visible zone of reaction by simultaneously reducing the concentration of antibodies and increasing the absolute amount of antigen for electrophoresis to 50–100 λl. Details of the method were worked out for human α-fetoprotein.
    No preview · Article · Sep 1979 · Bulletin of Experimental Biology and Medicine
  • G. I. Abelev · É. R. Karamova
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    ABSTRACT: A method of counterflow isotachophoresis of proteins on cellulose acetate films is suggested. To produce a counterflow of fluid, an electroendosmotic flow formed in the film during passage of an electric current through it was used. During isotachophoresis of proteins in the presence of ampholytes, the proteins are concentrated on the boundary between the leading and closing ions and, at the same time, they are fractionated by electrophoretic mobility. Microanalysis of protein mixtures in dilute solutions can be undertaken by this method. It is readily combined with immunodiffusion and immunoelectrophoretic detection of protein antigens.
    No preview · Article · Sep 1979 · Bulletin of Experimental Biology and Medicine
  • G I Abelev · A A Sokolenko
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    ABSTRACT: A highly sensitive electro-immunodiffusion test suggested by the authors for antigen detection on cellulose-acetate films consists of three stages: antigen concentration in a discontinuous buffer system on cellulose-acetate films; antigen detection on the same films by immunodiffusion using standard test system; to detect the precipitation bands the washed films are stained with protein dyes in case the reaction takes place in the zone of vision, or subject to further treatment by means of "plating" the precipitates with antiglobulin antibody or by radioautography. The method permits one to reveal the nanogram levels of alfa-fetoprotein and it may be applied for detection of antigens with different molecular weights and electrophoretic mobility.
    No preview · Article · Aug 1977 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • T L Eraĭzer · D A El'gort · G I Abelev
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    ABSTRACT: Population aspects of specific secreted proteins (alpha-fetoprotein and serum albumin) were analyzed in the cultured human embryo hepatocytes (6 to 12 weeks' gestation). A method based on local hemolysis in gel of sheep erythrocytes conjugated with antibodies specific of proteins in question. The great majority of individual hepatocytes synthesized both proteins.
    No preview · Article · Jul 1977 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • T. L. Éraizer · D. A. Él'gort · G. I. Abelev
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    ABSTRACT: Population aspects of the production of specific secreted proteins (serum albumin and a-fetoprotein) were studied in cultures of hepatocytes from human embryos at 6–12 weeks of development. A method based on local hemolysis in gel using sheep's erythrocytes conjugated with antibodies against the proteins for testing, was used. The overwhelming majority of hepatocytes were shownto synthesize both proteins.
    No preview · Article · Jun 1977 · Bulletin of Experimental Biology and Medicine
  • S D Perova · T L Eraizer · G I Abelev
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    ABSTRACT: Alpha-fetoprotein (AFP) produced by individual hepatocytes and hepatocyte microcolonies was detected with microelectrophoresis-precipitation in polyacrylamide gel. Hepatic cells of 6--13-week-old human embryos were cultivated in vitro for 2 to 5 days. 23 of 28 individual cells, and 89 of 91 microcolonies, built up of 2--35 cells, were demonstrated to produce AFP within the range of 70--800 pg per cell.
    No preview · Article · May 1977 · Biulleten' eksperimental'noĭ biologii i meditsiny
  • S. D. Perova · T. L. Éraizer · G. I. Abelev
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    ABSTRACT: By a combination of microelectrophoresis and precipitation in polyacrylamide gel, a-fetoprotein (a-FP) produced by single hepatocytes and by microcolonies of hepatocytes was determined. Liver cells from 6–13-week-old human fetuses were cultivatedin vitro for 2–5 days. a-FP was found to be produced in amounts of between 70 and 800 pg per cell by 23 of 28 single hepatocytes and by 89 of 91 microcolonies consisting of 2 to 35 cells
    No preview · Article · Apr 1977 · Bulletin of Experimental Biology and Medicine
  • O M Lejneva · G I Abelev · N.A. Dorfman · M Strand · J T August
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    ABSTRACT: A protein analogous to a 15,000-dalton structural protein of Rauscher murine oncornavirus has been localized on cell membranes of Rauscher erythroblastosis and CC57BR murine lymphosarcoma cells by use of monospecific antiserum in conjunction with immunofluorescence and immunoelectron microscopy techniques. Antigenic determinants of this protein were present on the membranes of erythroblastosis and lymphosarcoma cells, but were absent from the membrane of budding or mature virions. Both typespecific and group-specific determinants of the protein were accessible to antibodies reacting at the cell surface. This viral protein, expressed on the cell membrane, appears to correspond to previously described cell surface antigens of virus-induced murine leukemias.
    No preview · Article · Jan 1977 · Virology
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    ABSTRACT: alpha-FOETOPROTEIN (AFP) is a serum protein present in large amounts during embryonic life and preserved in only nanogram quantities in adult mammals1. Increased levels of AFP are seen in adults in association with two forms of tumours-teratocarcinomas and hepatocarcinomas-and also during liver regeneration2. In early development AFP is synthesised in the yolk sac and in the liver2,3 where it has been demonstrated by immunofluorescence in the majority of the hepatocytes4. Detailed aspects of AFP synthesis by hepatocytes, however, remain to be established. It has been suggested that it is synthesised by a definite population of hepatocytes5, by one of the stages in their differentiation6,7, or by liver cell precursors8. Sufficient data to decide between these alternatives are not presently available. We describe here the immunohistochemical localisation of AFP in regenerating mouse livers, in which DNA-synthesising cells have been identified by thymidine incorporation. AFP was found in a minority of adult differentiated hepatocytes, many of which had not progressed through S phase. Thus DNA synthesis is not a prerequisite for the induction of AFP synthesis in the adult liver.
    No preview · Article · Oct 1976 · Nature
  • E S Ievleva · N V Engelhardt · G I Abelev
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    ABSTRACT: A new antigan has been revealed by means of antisera against Rauscher virus in mice with Rauscher virus-induced leukemia. This antigen appears to be different from both Rauscher type-specific antigen and MULV-gs-1 (p-30), as shown by studies of electrophoretic mobility and immunochemical specificity. Except in leukemic mice it was also found in low levels in both serum and spleen extracts of healthy mice of a number of strains. Furthermore, this antigen was regularly demonstrated by immunofluorescence on the surface of erythroblasts, but not on the surface of erythrocytes, lymphocytes, polymorphonuclear cells and thymocytes, and was shown to be different from fetal hemoglobin. Therefore, it is referred to as antigen of erythroblasts (Ag-Eb), which seems to represent a surface marker for a certain differentiation stage of erythroid cells.
    No preview · Article · Jun 1976 · International Journal of Cancer
  • A A Sokolenko · G I Abelev
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    ABSTRACT: Alpha-fetoprotein (AFP) was detected in the sera of adult healthy persons by the method of electrophoresis-precipitation in polyacrylamide gel; it was distinctly determined in about 50% of cases, and its concentration was not over 3ng/ml. AFP was determined all the 20 cases suffering from cirrhosis of the liver examined by the mentioned method.
    No preview · Article · Apr 1976 · Biulleten' eksperimental'noĭ biologii i meditsiny

Publication Stats

3k Citations
222.89 Total Impact Points

Institutions

  • 2008
    • Russian Academy of Sciences
      • The N. N. Blokhin Cancer Research Center
      Moskva, Moscow, Russia
  • 1983-2008
    • N.N. Blokhin Cancer Research Center
      Moskva, Moscow, Russia
  • 1967-2002
    • Russian Academy of Medical Sciences
      Moskva, Moscow, Russia
  • 1962-1976
    • Research Institute of Epidemiology and Microbiology n.a. N. F. Gamalei, Russian Academy of Medical Sciences
      Moskva, Moscow, Russia
  • 1970-1972
    • International Agency for Research on Cancer
      Lyons, Rhône-Alpes, France