[Show abstract][Hide abstract] ABSTRACT: To investigate the expressions of Fas and Fas ligand (FasL) in human placenta,
we studied the expressions of Fas and FasL in placenta with RT-PCR, immunoblotting
and immunostaining. We observed amplified products of Fas and FasL transcripts,
the band of Fas (52 kDa) and multiple bands of FasL (42-52 kDa) in pla-centa.
Fas and FasL localized mainly on fetal vessels and on syncytiotrophoblasts
respectively. The differential distribution of Fas and FasL in human placenta may
reflect intrinsic expressions of them by trophoblasts during differentiation. The increased
expression of Fas in trophoblasts may promote apoptosis of placenta in
pathologic condition such as preeclampsia.
Full-text · Article · May 2002 · Journal of Korean Medical Science
[Show abstract][Hide abstract] ABSTRACT: This study was undertaken to investigate cyclin D2 mRNA and protein expression in human endometrium during the menstrual cycle.
Endometrial samples were obtained from 15 premenopausal nonpregnant women who had hysterectomies for benign gynecologic reasons. They were divided into the following five groups according to histologic dating: early proliferative (n = 3), mid to late proliferative (n = 3), early secretory (n = 3), mid secretory (n = 3), and late secretory (n = 3). Cyclin D2 mRNA and protein expression were analyzed using reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry.
Cyclin D2 mRNA and protein were expressed in human endometrial tissue throughout the menstrual cycle. Cyclin D2 mRNA and protein expression of proliferative phase endometrium were significantly higher than those of secretory phase endometrium (P <.05). The staining intensity of cyclin D2 in proliferative phase endometrium was higher than that in secretory phase (P <.05). Cyclin D2 mRNA level showed good correlation with cyclin D2 protein level (R = 0.579, P <.03), and cyclin D2 protein also showed good correlation with immunohistochemical staining intensity (R = 0.562, P <.03).
Cyclin D2 was expressed in human endometrium throughout the menstrual cycle. Cyclin D2 mRNA and protein were expressed at high levels in proliferative phase endometrium, especially in the early proliferative phase, and then decreased in the secretory phase.
No preview · Article · Feb 2002 · Journal of the Society for Gynecologic Investigation
[Show abstract][Hide abstract] ABSTRACT: Objective. Many investigators have studied the expression of G1 phase regulatory protein in uterine cervical cancer. However, it is unclear which step of the genetic expression participates in cyclin D1 expression and what its prognostic meaning is. The aims of this study were to evaluate the regulatory level of cyclin D1 expression and the relationship between the expression of cyclin D1 and its inhibitor, p21WAF1/CIP1, and to evaluate their impact on the prognosis of early stage cervical cancer.
No preview · Article · Jul 2001 · Gynecologic Oncology
[Show abstract][Hide abstract] ABSTRACT: Purpose: We carried out this study to evaluate the biological significance of phospholipase C β1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
Methods: Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) β1 and PLC β1−/− C57BL/6J × CBA F1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate–Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared.
Results: The mouse sperm null for the PLC β1 gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05). And the fertilization rate and the rate of embryos developed to the stage of morula or blastocyst were also lower in the group using PLC β1−/− mouse sperm compared to the intact group (P < 0.05; 73.5 vs 51.8% and 15.7 vs 4.3%, respectively).
Conclusions: Mutation of the PLC β1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC β1−/− mouse.
No preview · Article · Apr 2001 · Journal of Assisted Reproduction and Genetics
[Show abstract][Hide abstract] ABSTRACT: Purpose: This study was carried out to investigate the efficacyof electric stimulation before and/or after intracytoplasmicsperm injection (ICSI) on bovine oocyte activation andembryo development.
Methods: The oocytes were treated with electric shock before(B), before and after (B&A), and after (A) sperm injection.In each group, sham ICSI (ICSI-s) was performed to excludethe effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and AICSI-s). An electric pulse was applied with a single directcurrent (DC) pulse (0.8 kV/cm, 70 sec).
Results: One pronucleus (PN) formation in the B&A ICSI-sgroup was slightly higher than that found in B and B&AICSI group; however, the difference was not significant. TwoPN formation in B&A ICSI group was higher than that foundin sham ICSI groups (P < 0.05).="" there="" were="" no="" differencesamong="" treatment="" groups="" in="" the="" cleavage="" rate;="" however,="" morulaeand="" blastocyst="" formation="" in="" the="" b&a="" embryos="" wassignificantly="" higher="" than="" that="" of="" other="" groups="">P < 0.05)and="" got="">
Conclusions: Electric stimulation before and after injectionwas an effective method in inducing bovine oocyte activationand in sustaining embryo development to the morulae andblastocyst stage.
Full-text · Article · Jun 2000 · Journal of Assisted Reproduction and Genetics
[Show abstract][Hide abstract] ABSTRACT: Objectives. It has been shown that heat shock proteins (HSPs) protect cells from death caused by various noxious stimuli. Overexpression of HSP70 seems to be related to hormonal regulation of cell proliferation and/or down-regulation of sex steroid receptors. Wild-type p53 has been reported to repress HSP70 gene expression. It has been shown that mutant p53–HSP70 complex is highly expressed in cancer. However, the relationship between HSPs and steroid receptors or tumor suppressor gene products has not been well understood in uterine cervical carcinoma. This study was undertaken to examine the expression of HSP70, estrogen receptor (ER), and p53 in carcinoma of the uterine cervix. In addition, we analyzed HPV infection status and compared it to such immunohistochemical parameters. We also analyzed the relationship between these biological products and their clinicopathologic characteristics.Methods. Paraffin-embedded tissue sections were obtained from 84 patients with carcinoma of the uterine cervix. Expression of HSP70, p53, and ER was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody (SPA810), anti-p53 (BP53.12), and ER1D5 antibody, respectively. PCR HPV detection was done by dot hybridization method.Results. Positive staining of HSP70 was detected in 73% of the cases. HSP70 positivity was significantly higher in stage I cervical cancer than in stages II–IV (P = 0.02). This was associated with neither tumor size, lymph node status, parametrial involvement status, nor tumor markers (TA-4). Furthermore, there was no significant correlation between HSP70 positivity and the expression of p53 or ER or HPV infection status.Conclusion. These data suggested that HSP70 positivity was frequent in uterine cervical cancer, especially in the early stages. However, this was not significantly correlated with clinicopathologic characteristics nor with the expression of p53 or ER nor with HPV infection in carcinoma of the uterine cervix.
No preview · Article · Aug 1999 · Gynecologic Oncology