Ewa Ochwanowska

The Jan Kochanowski University in Kielce, Kel'tsy, Świętokrzyskie, Poland

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Publications (19)9.04 Total impact

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    ABSTRACT: Health-harming alcohol drinking leads to metabolic changes and, consequently, to an emergence of chemical compounds in the blood that can be considered as biological indicators of alcohol intake. These markers indicate both acute and chronic ethanol consumption. The aim of the present study was to determine the changes in the activity of the basic biochemical parameters indicative of alcohol abuse on admission and after 5-week hospitalisation assigned to subgroups by age and clinical variables.
    Preview · Article · Dec 2015 · Alkoholizm i narkomania
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    ABSTRACT: Combination therapy with pegylated interferon, ribavirin and a first-generation NS3/4A protease inhibitor, telaprevir or boceprevir, is the new strategy for treatment of genotype 1 chronic hepatitis C virus infection. This combination improves therapeutic efficacy but it also increases the risk of adverse events. The aim of the study was to analyze frequency and severity of dermatological adverse events during protease inhibitor-based therapy and to evaluate the risk factors for their development. This is a retrospective study of 109 patients with genotype 1 chronic hepatitis C treated with boceprevir (n=33) or telaprevir (n=76) based triple therapy. A logistic regression for relationship between clinical, demographic and laboratory factors and cutaneous adverse events was performed. Dermatological adverse events (skin rash, pruritus, anorectal paresthesia) occurred in both treatments (boceprevir and telaprevir) with similar frequency: 28% in telaprevir and 21% in boceprevir. In patients treated with telaprevir, men were more predisposed to develop skin rashes compared to women (OR 4,1 p=0,014) and age above 45 years was associated with occurrence of pruritus in men (OR 8,16 p=0,014). Being a female, coexistence of autoimmune thyroiditis and advanced liver fibrosis were independent factors predisposing to development of anorectal paresthesia (OR 4,13 p=0,041, OR 4,25 p=0,029, OR 4,54 p=0,018 respectively) in this group. In patients treated with boceprevir, coexistence of autoimmune thyroiditis predisposed to skin rashes (OR 10,22 p=0,017) and being a female predisposed to pruritus (OR11,2 p=0,033). The adverse events occurred after a mean time of 8,6 (range 1-24) weeks after initiation of therapy. In patients with chronic hepatitis C who received the triple therapy, the anorectal paresthesias were observed only in patients treated with telaprevir. The predisposing factors for this adverse event were: female gender and advanced liver fibrosis. The risk factors for other dermatological adverse were: 1) being a male over 45 years, for skin rashes and pruritus (for telaprevir), 2) coexistence of autoimmune thyroiditis for skin rashes (for boceprevir), 3) being a female, for pruritus (for boceprevir).
    No preview · Article · Dec 2014 · Journal of Dermatological Case Reports
  • Bozena Witek · Ewa Ochwanowska · Artur Wrobel · A. Kolła̧taj
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    ABSTRACT: The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experimental groups (A-F) with 250 μl 0.9% NaCl solution containing 20 or 30 mg morphine hydrochloride per kg body weight. The injections were given intramuscularly once a day between 9:00-10:00 a.m. for 4, 10 and 14 days. In the lysosomal fraction of the liver and kidney the activities of acid phosphatase, lysosomal esterase, β-glucuronidase, β-galactosidase, and β-N-acetyl-hexosaminidase were estimated. Morphine increased the activity of all examined enzymes except EL, which activity was statistically proven to decrease in liver and kidneys after 10 days morphine administration in both doses.
    No preview · Article · Jan 2011 · Animal science papers and reports
  • Bozena Witek · Ewa Ochwanowska · Artur Wrobel · A. Kołataj
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    ABSTRACT: Used were ninety Swiss mice males divided into 9 groups (n=10). Three control groups [I, II, III] were injected with 0.9% NaCl solution, while six experimental groups (A, B, C, D, E, F) with the morphine hydrochloride. Mice from groups A, C and E were injected with a dose of 20 and those from B, D and F with a dose of 30 mg morphine per kg body weight. In each group, both solutions were administered intramuscularly once a day from 9:00 to10:00 a.m. for 4, 10 and 14 days. In the lysosomal fraction of the liver and kidney the activities of cathepsin D, cathepsin L, alanine aminopeptidase, leucine aminopeptidase, lysosomal lipase, and β-glucosidase were estimated. Morphine led to the increased activity of all examined enzymes except lysosomal lipase, the activity of which dropped in both organs examined.
    No preview · Article · Jan 2010 · Animal science papers and reports
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    Ewa Ochwanowska · Bożena Witek · Adam Kołątaj
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    ABSTRACT: The experiment was carried out on 20 Swiss male mice divided into experimental (E, n=10) and control (C, n=10) group. E mice were intraperitoneally injected with glucagon (15 μg/kg b.w.) twice daily for eight days, while mice C with 250 μl 0.9% NaCl/mouse (to exclude the injection effect only). In the lysosomal fraction of the liver the activities of acid phosphatase, β-N-acetyl-hexosaminidase, β-glucuronidase, β-glucosidase, lysosomal arylesterase, lysosomal lipase, leucine aminopeptidase and alanine aminopeptidase were determined. Glucagon caused an increase of activity of all studied enzymes except β-glucuronidase and β-glucosidase, where reduced activities were observed. KEY WORDS: adaptation / glucagon / glucose / lysosomal enzymes / mice Endocrine pancreatic functions in the course of diabetes were a subject of numerous studies [Cryer 2008, Henkel et al. 2005]. The lysosomal enzymes are important in the cell degradation processes [Beaujouin and Liaudet-Coopman 2008, Minazaki et al. 2008, Witek et al. 2007, 2008]. The participation of lysosomal enzymes in glucagon-induced autophagocytosis was first suggested by Ashford and Porter [1962]. It has been found that the formation of autophagic vacuoles after glucagon treatment in vivo was accompanied by an increase in fragility and osmotic sensitivity of lysosome membranes [De Duve and Wattiaux 1966, Deter and De Duve 1967].
    Full-text · Article · Jan 2009 · Animal science papers and reports
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    Bożena Witek · Ewa Ochwanowska · Adam Kołątaj
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    ABSTRACT: The investigations were conducted on 20 Swiss male mice, fed a feed containing 16% (control group, n=10) or 10% protein (experimental group, n=10). After 14 days of feeding, sections of liver and kidneys were obtained from all animals and homogenized in order to obtain the lysosomal fraction, in which the activity of the following enzymes was determined: acid phosphatase, lysosomal esterase, lysosomal lipase, β-glucuronidase, β-galactosidase, β-glucosidase, β-N-acetyl-hexosaminidase, leucine aminopeptidase, alanine aminopeptidase and cathepsins D and L. Feed with the protein level reduced to 10% had, compared to the control, a significant and differentiated effect on the activity of enzymes examined, depending on the enzyme type and organ.
    Full-text · Article · Jan 2008 · Animal science papers and reports
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    ABSTRACT: The experiment was conducted on the influence of testosterone on the activity of ten lysosomal enzymes in the mouse liver and kidney. Mice were divided into two feeding group [control - 16% protein] and [10% protein] in diet. The animals of the both groups were injected with 0.05 mg/kg b.w. of testosterone intraperitoneally. In the lysosomal fraction of the liver and kidney the activity of lysosomal enzymes were estimated. Testosterone decreased activity of all investigated enzymes in the liver, except alanine aminopeptidase, cathepsins D and L, lysosomal lipase. In the lysosomal fraction of the kidney testosterone decreased of all lysosomal enzymes, too, except alanine aminopeptidase. The results suggest that exogenous testosterone and different proteins diet had a significant influence on the activity of investigated lysosomal enzymes.
    No preview · Article · Sep 2007 · Medycyna weterynaryjna
  • B. Witek · E. Ochwanowska · D. Baranowska · A. Koła̧taj
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    ABSTRACT: The study was conducted on 20 Swiss line male mice divided into two groups - control (n=10) comprised of unselected animals (group C), and experimental (n=10) comprised of mice selected over 24 generations for high live body weight gain (group S). Beginning from day 42 of life mice of both groups received over a period of 14 days a standard pelleted feed containing 16% protein. After 14 days the animals were killed, a lysosomal fraction of liver and kidney cells was obtained and analysed for the activity of acid phosphatase, lysosomal esterase, lysosomal lipase, β-glucuronidase, β-galactosidase, β-glucosidase, β-N-acetylo-hexosaminidase, leucine aminopeptidase, alanine aminopeptidase and cathepsins D and L. In S mice the activities of a majority of the lysosomal hydrolases examined were found to be higher than in C animals.
    No preview · Article · Jan 2007 · Animal science papers and reports
  • B. Witek · A. Graniczka · E. Ochwanowska · A. Kołataj
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    ABSTRACT: Changes in the activity of β-glucuronidase, N-acetyl-β- glucosaminidase, cathepsin D and cathepsin L in the liver and kidney of mice, injected intraperitonealy with 15 μg/kg b.w. of glucagon were investigated. The experiment was carried out on 24 8-week-old mice, whose parents were chosen from random match. The homogenates of the liver and kidney were subjected to differentiate centrifuging, sad in the lysosomal fractions of the liver and kidney the activities of β-glucuronidase [β-GlcUr], N-acetyl-β-glucosaminidase [NAG], cathepsin D [Cath. D], and cathepsin L [Cath. L] were estimated. Injection of glucagon caused a decrease in the activity of β-glucuronidase in the liver and kidney, cathepsin L in the liver, N-acetyl-β-glucosaminidase in the kidney, and an increase cathepsin D and cathepsin L in the kidney of mice. The results suggest that exogenous glucagon had a significant influence on the activity investigated lysosomal enzymes. The range of the reaction remained in a relationship with the kind of the organ and type of enzyme.
    No preview · Article · Jun 2006 · Medycyna weterynaryjna
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    ABSTRACT: The studies have been conducted on 40 male mice. The experiment was connected with the influence of B1, B2 and B6 vitamins on some lysosomal enzyme activities in the liver and kidney. The thiamin injection decreased lysosomal esterase, lysosomal lipase and alanine aminopeptidase activities in the liver; the ryboflavin injection - lysosomal esterase and alanine aminopeptidase, and pyridoxine injection - lysosomal esterase, cathepsin D and L and alanine aminopeptidase. Ryboflavin increased the activity of β-glucuronidase, N-acetyl-β-glucosaminidase, acid phosphatase and leucine aminopeptidase in the liver; pyridoxine - N-acetyl-β-glucosaminidase and leucine aminopeptidase activity. Thiamin increased β-glucuronidase, β-galactosidase, N-acetyl-β- glucosaminidase, acid phosphatase, lysosomal lipase, cathepsin D and L, leucine aminopeptidase and alanine aminopeptidase activity in the kidney; ryboflavin -β-glucuronidase, β-galactosidase, N-acetyl-β-glucosaminidase, acid phosphatase, lysosomal lipase, cathepsin D and L, leucine aminopeptidase and alanine aminopeptidase activity, while pyridoxine -β-glucuronidase, β-galactosidase, N-acetyl-β-glucosaminidase, acid phosphatase, leucine aminopeptidase and alanine aminopeptidase. Vitamins B1, B 2 and B6 only decreased lysosomal esterase activity in the kidney. In comparison with the liver, the kidney revealed the greater reactivity of the studied lysosomal enzymes.
    No preview · Article · Mar 2006 · Medycyna weterynaryjna
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    ABSTRACT: Observing the changes of activity of some lysosomal enzymes in blood serum of female rabbits subjected to injection of 10 microg of ghrelin/kg of body weight. In the blood serum the activity of cathepsins D and L, alanine aminopeptidase, acid phosphatase, lysosomal lipase and lysosomal esterase was determined. As a result of ghrelin injection the activity of all the enzymes examined in blood serum increased markedly. Changes of lysosomal enzymes activities in the blood serum caused by the effects of ghrelin should be regarded as the response of the lysosomal system.
    Full-text · Article · Sep 2005 · Neuro endocrinology letters
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    ABSTRACT: MALDI-TOF mass spectrometry, 1H NMR spectrometry, the continuous variation method and molecular modeling by MM3 calculation confirmed our earlier studies showing that gonadotropin-releasing hormone (GnRH) forms complex with copper(II) ion with the binding ratio 1:1. The copper(II) complex formed at physiological pH has a square planar configuration and GnRH complexes with nickel(II) and cobalt(II) ions are less stable than that of copper(II).
    Full-text · Article · Jul 2005 · Neuro endocrinology letters
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    ABSTRACT: Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w. insulin for 4 and 8 days. The homogenates of the liver and kidney were taken for examination. The activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L has been determined according to [1] method. The activity of alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L in the liver and kidney of male and female of mice decreased in effect of insulin injections for 4 and 8 days. The changes of enzyme activities showed a stimulating effect of the insulin injection on the labilization of lysosomal membranes. The range of the reaction remained in a relationship with the kind of the organ, the type of enzyme, time over which insulin introduced operates in the organism, and with the sex.
    Full-text · Article · Feb 2004 · Neuro endocrinology letters

  • No preview · Article · Feb 2004
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    Bozena Witek · Ewa Ochwanowska · Alina Slewa · Adam Kolataj
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    ABSTRACT: Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w. of hydrocortisone injection for 4 and 8 days. The homogenates of the liver and kidney were subjected to differentiated centrifuging and determination of studied enzymes. Injection of hydrocortisone caused an increase in the activity of all investigated lysosomal enzymes in the liver and kidney of mice. The reactions of selected mice were stronger in comparison with unselected ones. The highest increase in the activity investigated enzymes was observed after 8 days of hydrocortisone injection.
    Full-text · Article · May 2002 · Neuro endocrinology letters
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    B Witek · T Król · A Kołataj · E Ochwanowska · I Stanisławska · A Slewa
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    ABSTRACT: The study was carried out on fifty male rabbits of the New Zealand White breed. Diabetes was caused by a single, intravenous alloxan injection. Rabbits which had glycaemia 7th day after the alloxan administration higher than 11 millimol/litre were selected for the studies. They were divided into 5 groups: I - control (without diabetes); II - 3-week diabetes; III - 6-week diabetes; IV - 3-month diabetes; V - 6-month diabetes. In control and experimental rabbits the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, lysosomal acid phosphatase, leucine aminopeptidase, cathepsin D, and lysosomal arylesterase was determined in lysosomal fractions of the liver and kidney. Alloxan caused lowering of the activity of all the investigated enzymes in the kidney and liver except lysosomal arylesterase. Alloxan injection caused a significant increase in the activity of all the investigated enzymes. The advisable lysosomal enzymes may be useful for the monitoring of the course and effectiveness of diabetes therapy.
    Full-text · Article · Sep 2001 · Neuro endocrinology letters
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    B Witek · E Ochwanowska · A Kolataj · A Slewa · I Stanislawska
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    ABSTRACT: Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated. The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes. Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney. Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups.
    Full-text · Article · Jul 2001 · Neuro endocrinology letters
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    Bozena Witek · Ewa Ochwanowska · Adam Kolataj · Alina Slewa
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    ABSTRACT: OBJECTIVES: Changes in the activity of β-glucuronidase, N-acetyl-β- glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxy- tryptamine) for 7 and 14 days were investigated. METHODS: The slices of the liver and kidney were homogenized in 0. 1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes. RESULTS: Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney. CONCLUSION: Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups.
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  • AGATA GRANICZKA · EWA OCHWANOWSKA · ADAM KO
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    ABSTRACT: Witek B., Graniczka A., Ochwanowska E., Ko‡"taj A. Changes of the lysosomal enzyme activity in the liver and kidney of mice after glucagon injections Summary Changes in the activity of b-glucuronidase, N-acetyl-b-glucosaminidase, cathepsin D and cathepsin L in the liver and kidney of mice, injected intraperitonealy with 15 µg/kg b.w. of glucagon were investigated. The experiment was carried out on 24 8-week-old mice, whose parents were chosen from random match. The homogenates of the liver and kidney were subjected to differentiate centrifuging, and in the lysosomal fractions of the liver and kidney the activities of b-glucuronidase (b-GlcUr), N-acetyl-b-glucosaminidase (NAG), cathepsin D (Cath. D), and cathepsin L (Cath. L) were estimated. Injection of glucagon caused a decrease in the activity of b-glucuronidase in the liver and kidney, cathepsin L in the liver, N-acetyl-b-glucosaminidase in the kidney, and an increase cathepsin D and cathepsin L in the kidney of mice. The results suggest that exogenous glucagon had a significant influence on the activity investigated lysosomal enzymes. The range of the reaction remained in a relationship with the kind of the organ and type of enzyme.
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