- [Show abstract] [Hide abstract] ABSTRACT: The article discusses a possible participation of E. coli in the pathogenesis of inflammatory bowel diseases. Bacteria, especially opportunistic, may play a role as one of the possible aetiologic agents in the initiation of the inflammatory reaction in the colon. E. coli is predominant microorganism in the initiation of early and chronic ileal lesions of Crohn disease, ulcerative colitis and colorectal carcinoma. E. coli strains isolated from biopsy specimens of colon of patients with inflammatory and tumor bowel diseases are often associated to mucosa of colon. They are able both to adhere and invade epithelial cells of colon in vitro. These strains belong to a potentially pathogenic group of invasive E. coli, which was designated as a group of adherent-invasive E. coli. Mechanism of their pathogenicity reflects their capability to adhere onto epithelial cells of colon and following mucosal colonisation. The colonisation is mediated by adhesins at the surface of the bacteria, especially type 1 pili and specific receptors at the surface of the host cells. E. coli strains enter the host cells using a way similar to macropinocytosis and multiply in the cytoplasm. They are able to replicate within macrophages without their killing. The invasive process of adherent-invasive E. coli is original, in that they possess none of the known genetic invasive determinants.
- [Show abstract] [Hide abstract] ABSTRACT: In total, 201 alpha-haemolytic Escherichia coli isolates from various clinical materials (urine samples and vaginal and rectal swabs) were examined by PCR for the presence of genes for the virulence factors alpha-haemolysin (hly), cytotoxic necrotising factor type 1 (cnf1), P-fimbriae (pap), S/F1C-fimbriae (sfa/foc), aerobactin (aer) and afimbrial adhesin (afaI). Among vaginal isolates, 96% were positive for cnf1, compared with 80% of urine strains (p 0.02) and 63% of rectal strains (p 0.0001). Similarly, sfa/foc-specific DNA sequences were found in 97% of vaginal isolates compared with 75% of rectal strains (p 0.004). The afa1 and aer genes were associated more with rectal alpha-haemolytic E. coli strains than with extra-intestinal isolates. The results suggested that CNF1 and/or S/F1C-fimbriae contribute to colonisation and persistence of alpha-haemolytic E. coli strains in the vaginal environment.
- [Show abstract] [Hide abstract] ABSTRACT: In this work we point out an occurrence of non-albicans Candida species isolated from catheters, cannulas and drains. We detected eight non-albicans Candida species in 49 examined samples: C. parapsilosis (n = 26), C. tropicalis (n = 12), C. krusei (n = 4), C. claussenii, C. mesenterica (n = 2 for each), C. guilliermondii, C. kefyr and C. lusitaniae (n = 1 for each). Material examined from children hospitalized in intensive care units was the most frequent. Eight samples were from the oncology department, seven from the surgery department, six from the anaesthesiology department, four from the dialysis unit, two from the hematology department, one from the internal medicine department and one from the stomatology department. We examined cannula scraping 26 times (53%), catheter scraping 14 times (28.6%) and drain scraping nine times (18.4%).
Article: Candidosis in neonates[Show abstract] [Hide abstract] ABSTRACT: Neonatal candidosis is the main mycotic infection in infants calling for intensive care. Subjects of the presented study were preterm infants admitted to the neonatal intensive care unit between January 1995 and June 1998. The yeast-like organisms were diagnosed by their morphological and biochemical properties. From the clinical material most frequently C. albicans (42.7 %), C. parapsilosis (35.7 %), C. tropicalis (6.9 %), C. claussenii and C. guilliermondii (3.5 % each) were isolated. In 4.7 % other species were detected. The highest incidence of yeast-like, microorganisms was recorded in the oral cavity, on the skin and rectum.
- [Show abstract] [Hide abstract] ABSTRACT: In Escherichia coli strains isolated from faeces and smears from the rectum in haemolytic-uraemic syndrome of a child and healthy relatives the authors assessed the biochemical activity, antigenic characteristic, sensitivity to twelve antimicrobial substances and factors of virulence. Sorbitol negative strains were isolated from the ill child and his father. The strains belonged into serogroup 016. The remaining strains were sorbitol positive. They belonged into serogroups 02, 012 and 019. Nineteen sorbitol negative strains of E. coli from the sick child caused mannose sensitive agglutination of chicken and guinea pig erythrocytes, they produced colicin V and aerobactin. Except for one strain they were serum resistant. Fourteen strains induced the oedema on the foot of mice and eleven produced a lethal effect in mice. Except for three ampicillin resistant strains the remaining were sensitive to the examined antibacterial substances. Two sorbitol negative strains of E. coli isolated from the asymptomatic father produced aerobactin and one strain was serum resistant.
- [Show abstract] [Hide abstract] ABSTRACT: In a group of 50 strains of E. coli isolated from infants hospitalized on account of diarrhoea the authors investigated, based on the phenotypic manifestation and in a selected number of strains also genotypic factors of virulence: fimbrial adhesins, aerobactin, haemolysins, colicins, toxic and lethal action in mice, seroresistence and resistance against tetracycline, streptomycin, chloramphenicol, kanamycin, ampicillin and sulfamethoxidine. All strains had one to eight factors of virulence. The presence of plasmid DNA was confirmed in 14 of 16 tested strains. From this the authors conclude that the investigated virulence factors were in the majority coded plasmids.