Hye Young Son

University of Seoul, Sŏul, Seoul, South Korea

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Publications (9)18.2 Total impact

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    ABSTRACT: Somatic cell nuclear transfer (SCNT) is an alternative potential tool for the conservation of endangered. In this study, somatic cells were collected from a purebred 9-month-old male mastiff and an 11-month-old female mastiff. Oocytes that had been matured in vivo were retrieved from outbred dogs by laparoscopy. We used cycling cells as donor cells for SCNT. A total of 289 oocytes were reconstructed with each male or female somatic cell and then fused/activated simultaneously by electrical stimulation. Finally, 224 embryos were transferred to 16 recipients that had been synchronized naturally. The efficiency of delivery of cloned dogs (7.1%) was threefold higher than in previous reports. Moreover, one surrogate delivered four identical cloned female Tibetan Mastiff puppies; another three surrogates each delivered triplets. Microsatellite analysis demonstrated the genotypic identity of the cloned puppies. Thus, our study has demonstrated techniques that improve significantly the overall efficiency of SCNT in the canine species.
    No preview · Article · Jan 2012 · Pakistan Veterinary Journal
  • Wan Soon Kim · Na Hee Yoon · Hye young Son · Ji Hyun Seo
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    ABSTRACT: The objective of this study was to determine the effects of light intensity and nutrient solution on the growth of indoor plants in the vertical gardening system. The four common indoor plants of Hedera helix, H. canariensis 'Variegata', Epipremnum aureum, and E. aureum 'Lime' were used in this experiment. The experiment was conducted eight weeks under the two different intensity of fluorescent lighting, 40 and 80 PAR, for 12 hours a day in the indoor condition. The nutrient treatment consisted of three levels: no supply (tap water below EC 0.1 as control) and EC 0.5 and EC 1.0 adjusted by Hoagland's solution modified for foliar plants. As results, the plant growth typified by leaf number and leaf area was better in the low light intensity with nutrient supplement of EC 0.5 or EC 1.0 in both of Epipremnum spp. and Hedera spp. than in the high light intensity without any nutrient supply (control). Under the same light intensity of 40 or 80 PAR, Epipremnum showed the largest biomass in EC 0.5 nutrient solution while Hedera did in EC 1.0 nutrient solution. Total nitrogen content was more in low light intensity-EC 0.5 and EC 1.0 than in high light intensity without any nutrient supply (control). In conclusion, the nutrient supplement at the relatively low light intensity was more effective to plant growth than at no nutrient supply (control). These results suggest that supplemental nutrients could be compensate the poor light condition for the growth of indoor foliar plants.
    No preview · Conference Paper · Sep 2011
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    ABSTRACT: Some human embryonic stem cell lines have shown genomic instabilities over long-term culture. To study the controversial origin of the SCNT-hES-1 line, which was derived from autologous somatic cell nuclear transfer (SCNT), we compared the expression and methylation patterns of imprinted genes in the SCNT-hES-1 cells with the donor's somatic cells by semi-quantitative RT-PCR, real-time PCR and bisulfite sequencing. Examined imprinted genes were H19, GNAS, SLC22A18, UBE3A and ZNF264 for maternally expressed genes, and IGF2, SNRPN, PEG3, PEG10, MEST, MAGEL2 and ARHI for paternally expressed genes, respectively. We found that the expression of imprinted genes in the SCNT-hES-1 cell line is comparable to that in the donor's somatic cells, and that its methylation patterns are similar to those of other SCNT-products. Therefore, the present study indicates that the SCNT-hES-1 line was derived from SCNT.
    Full-text · Article · Aug 2011 · International Journal of Molecular Medicine
  • Hye Young Son · Hyo Won Jung · Won-Ki Kim · Yong-Ki Park
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    ABSTRACT: Endothelial dysfunction is involved in stroke. Recent therapeutic options for stroke have focused on the combination therapy with a polyherbal mixture. This study was designed to provide insight into the effects of JP05, a water extract of 12 herbs, on the levels of regulators in bEnd.3 mouse brain endothelial cells. Production of endothelial nitric oxide synthase (eNOS)-mediated nitric oxide (NO), the expression of vascular endothelial growth factor (VEGF) and the phosphorylations of eNOS, phosphatidylinositol 3-kinase (PI3K)/Akt, extracellular signal-regulated protein kinase (ERK) and cAMP response element binding protein (CREB) in JP05 were assayed in bEnd.3 cells, a mouse brain endothelial line. JP05 led to increase the levels of eNOS-mediated NO generation and VEGF expression in bEnd.3 cells. JP05 induced the phosphorylation of eNOS, Akt and ERK in bEnd.3 cells. As well, JP05 blocked the inhibition of PI3K/Akt and ERK activities by LY294002 (PI3K/Akt inhibitor) and PD98059 (mitogen-activated protein kinase inhibitor), respectively. JP05 also induced the phosphorylation of CREB, which plays an important role in endothelial cell function and blood vessel development. Taken together, these results indicate that JP05 can upregulate eNOS-mediated NO generation and VEGF expression through the ERK and/or PI3K/Akt activation, an upstream event of angiogenesis. JP05 with vasoprotective properties has a potential therapy for human brain diseases including stroke.
    No preview · Article · Aug 2010 · Journal of ethnopharmacology
  • Hyo Won Jung · Hye Young Son · Guang-Zhen Jin · Yong-Ki Park
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    ABSTRACT: Many current studies of Parkinson's disease (PD) suggest that inflammation is involved in the neurodegenerative process. PD-1, a traditional Korean medicine, used to treat various brain diseases in Korea. This study was designed to investigate the effect of PD-1 extract in the Parkinson's model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) lesioned mice. The MPTP administration caused the dopamine neuron loss in the striatum and substantia nigra pars compacta (SNpc), which was demonstrated by a depletion of tyrosine hydroxylase (TH). In addition, a reduction of bcl-2 expression with elevation of bax expression, caspase-3 activation, and release of cytochrome c into cytosol in dopaminergic neurons of SNpc were noted. Oral administration of PD-1 extract (50 and 100 mg kg(-1)) attenuated the MPTP-induced depletion of TH proteins in the striatum and SNpc and prevented the apoptotic effects. These results indicate that PD-1 extract is able to protect dopaminergic neurons from MPTP-induced neuronal death, with important implications for the treatment of PD.
    No preview · Article · Apr 2010 · Cell Biochemistry and Function

  • No preview · Article · Jan 2010 · Reproduction Fertility and Development
  • Hye Young Son · Hyung Soo Han · Hyo Won Jung · Yong-Ki Park
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    ABSTRACT: The roots of Panax notoginseng (PN) are commonly used as a therapeutic agent to stop hemorrhage and as a tonic to promote health in traditional Korean medicine. Stroke triggers an inflammatory response that not only plays a central role in the pathogenesis of cerebral ischemia, but also induces secondary damage. This study was designed to investigate the neuroprotective effects of the methanol extract of PN on the infarct volume induced by middle cerebral artery occlusion (MCAO) (90-min occlusion and 24-h reperfusion) in rat brains. The PN extract (50 mg/kg, i.p.) was administered 2 h after the onset of MCAO. The PN-treated groups had a reduction in infarct volume by 23.82 +/- 8.9%. In the PN extract-treated groups, the microglial density was significantly decreased in the peri-infarct region; the underlying mechanism was inhibition of inflammatory mediators, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, via blocking of the NF-kappaB pathway. Furthermore, in vitro studies showed that the PN extract significantly reduced the production of iNOS-derived NO and COX-2-derived prostaglandin E(2) through the regulation of gene transcription levels in primary microglia and BV-2 cells. These results suggest that anti-inflammatory and microglial activation inhibitory effects of the PN extract may contribute to its neuroprotective effects in brain ischemia.
    No preview · Article · Apr 2009 · Journal of Pharmacological Sciences
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    ABSTRACT: Excessive production of inflammatory mediators, nitric oxide (NO) and proinflammatory cytokines from activated microglia has been implicated in neurodegeneration in human brain diseases. Recently, it seems possible that treatment with antiinflammatory agents, including Oriental medicinal plants, might delay the progression of neurodegeneration through the inhibition of microglial activation. The present study evaluated the effect of a methanol extract of Ficus religiosa leaf (MFL) on lipopolysaccharide (LPS)-induced production of NO and proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-beta (IL-1beta) and IL-6 in BV-2 cells, a mouse microglial line. MFL inhibited LPS-induced production of NO and proinflammatory cytokines in a dose-dependent manner. MFL also attenuated the expression of mRNA and proteins of inducible nitric oxide synthase (iNOS) and proinflammatory cytokines, suggesting the blockage of transcription levels, respectively. The molecular mechanism of MFL-mediated attenuation underlies the down-regulation of the extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) signaling pathway, and suppresses the nuclear factor kappaB (NF-kappaB) activation. The results suggest that MFL exhibits antiinflammatory properties in LPS-induced activation of BV2 microglial cells, and that might have a therapeutic potential for various neurodegenerative diseases.
    No preview · Article · Aug 2008 · Phytotherapy Research
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    ABSTRACT: Xenotransplantation is a rapidly expanding field of research and cloned miniature pigs have been considered as a model animal for it. However, the efficiency of somatic cell nuclear transfer (SCNT) is extremely low, with most clones resulting in early lethality and several kinds of aberrant development. A possible explanation for the developmental failure of SCNT embryos is insufficient reprogramming of the somatic cell nucleus by the oocyte. In order to test this, we analyzed the reprogramming capacity of differentiated fibroblast cell nuclei and embryonic germ cell nuclei with Oct-4 and Oct-4 related genes (Ndp5211, Dppa2, Dppa3, and Dppa5), which are important for embryonic development, Hand1 and GATA-4, which are important for placental development, as molecular markers using RT-PCR. The Oct-4 expression level was significantly lower (P<0.05) in cloned hatched blastocysts derived from fibroblasts and many of fibroblast-derived clones failed to reactivate at least one of the tested genes, while most of the germ cell clones and control embryos correctly expressed these genes. In conclusion, our results suggest that the reprogramming of fibroblast-derived cloned embryos is highly aberrant and this improper reprogramming could be one reason of the early lethality and post-implantation anomalies of somatic cell-derived clones.
    No preview · Article · Oct 2006 · Biochemical and Biophysical Research Communications

Publication Stats

95 Citations
18.20 Total Impact Points


  • 2011
    • University of Seoul
      • Department of Environmental Horticulture
      Sŏul, Seoul, South Korea
  • 2010-2011
    • Sooam Biotech Research Foundation
      Sŏul, Seoul, South Korea
  • 2008-2010
    • Dongguk University
      • Oriental Medicine R&D Center
      Sŏul, Seoul, South Korea
  • 2006
    • Seoul National University
      • Department of Agricultural Biotechnology
      Sŏul, Seoul, South Korea