Anna Goździcka-Józefiak

Adam Mickiewicz University, Posen, Greater Poland Voivodeship, Poland

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Publications (84)72.97 Total impact

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    ABSTRACT: Background: The exact route of human papillomavirus (HPV) and herpes simplex virus (HSV) transmission from a pregnant woman to her fetus has not been clearly established thus far. The data of many studies raise the possibility of intrauterine infection. In order to clarify our knowledge about virus vertical transmission in pregnant women, viral prevalence in amniotic fluid cannot. To the best of our knowledge, this is the first study on HPV DNA and HSV DNA detection in amniotic fluid in Poland. Material and methods: The study covered 138 samples of amniotic fluid from patients undergoing invasive prenatal diagnostic procedures (for medical indications) during the second trimester of gestation. The aim of the study was to assess the prevalence of HPV and HSV in the amniotic fluid samples obtained from asymptomatic women with intact amniotic membranes. To identify viral DNA of HPV and HSV in collected material, polymerase chain reaction (PCR) was performed. Results: We did not find HPV or HSV DNA in any of the examined specimens of amniotic fluid. Conclusion: Our investigation did not confirm the prenatal transmission of HPV and HSV to the amniotic fluid.
    No preview · Article · Dec 2015 · Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine)
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    Mariusz Skoczyński · Anna Goździcka-Józefiak · Anna Kwaśniewska
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    ABSTRACT: The impact of human papillomavirus (HPV) infection on pregnancy is a major problem of medicine. The transmission of the virus from mother to fetus is a process yet unresolved. The immune response and changed hormonal status of pregnant women might facilitate infection. A research on the prevalence of HPV infection was conducted at the Clinic of Obstetrics, Medical University of Lublin (Poland). The studied group included 152 randomly selected women. The material was tested for the presence of HPV DNA by means of polymerase chain reaction (PCR). The aim of the research was to assess the relation between HPV infections detected in the buccal smears of the neonates and the incidence of such infections in the cervical/buccal smears of their mothers. In the group of 152 infants HPV was found in 16 (10.53%). Among the cervical/buccal smears, HPV was isolated, respectively, in 24 (15.79%) and in 19 (12.5%) pregnant women. Statistically significant differences in the prevalence of HPV swabs from the newborns and the cervical/buccal smears of their mothers were found ( p < 0.001). The identification of mothers in whose buccal smears HPV was detected can help develop a group of children who run a relatively significant risk of being infected.
    Full-text · Article · Dec 2015
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    Julia Durzyńska · Anna Goździcka-Józefiak
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    ABSTRACT: Many attempts have been made to define nature of viruses and to uncover their origin. Our aim within this work was to show that there are different perceptions of viruses and many concepts to explain their emergence: the virus-first concept (also called co-evolution), the escape and the reduction theories. Moreover, a relatively new concept of polyphyletic virus origin called "three RNA cells, three DNA viruses" proposed by Forterre is described herein. In this paper, not only is each thesis supported by a body of evidence but also counter-argued in the light of various findings to give more insightful considerations to the readers. As the origin of viruses and that of living cells are most probably interdependent, we decided to reveal ideas concerning nature of cellular last universal common ancestor (LUCA). Furthermore, we discuss monophyletic ancestry of cellular domains and their relationships at the molecular level of membrane lipids and replication strategies of these three types of cells. In this review, we also present the emergence of DNA viruses requiring an evolutionary transition from RNA to DNA and recently discovered giant DNA viruses possibly involved in eukaryogenesis. In the course of evolution viruses emerged many times. They have always played a key role through horizontal gene transfer in evolutionary events and in formation of the tree of life or netlike routes of evolution providing a great deal of genetic diversity. In our opinion, future findings are crucial to better understand past relations between viruses and cells and the origin of both.
    Full-text · Article · Oct 2015 · Virology Journal
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    Full-text · Dataset · Oct 2015
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    Full-text · Dataset · May 2015
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    ABSTRACT: Efficient delivery of heterologous molecules for treatment of cells is a great challenge in modern medicine and pharmacology. Cell penetrating peptides (CPPs) may improve efficient delivery of a wide range of macromolecular cargos including plasmid DNA, small interfering RNA, drugs, nanoparticulate pharmaceutical carriers and anticancer drugs. In this paper, we present history of CPPs' discovery with a special attention drawn to sequences of viral origin. We also describe different CPP families with regard to their physicochemical properties and numerous mechanisms of CPP cell uptake by direct penetration and endocytotic pathways. A detailed description is focused on formation of carrier-cargo complexes, which are needed for practical use of CPP in medicine and biotechnology. Examples of successful application of CPPs in treatment of human diseases are also presented, including decreased tumor growth and induction of cancer cell death. Finally, we review modern design approaches to novel CPPs and prediction of their activity. To sum up, the current review presents a thorough and up-to-date knowledge about CPPs and may be a valuable source of information for researchers in pharmacology designing new therapeutic agents. The American Society for Pharmacology and Experimental Therapeutics.
    Full-text · Article · Apr 2015 · Journal of Pharmacology and Experimental Therapeutics
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    ABSTRACT: Microbial communities living in stratified lakes are known to be early indicators of ecosystem disturbances. Based on information available up to date it was hypothesized that structures of heterotrophic bacteria and phytoplankton assemblages in the eutrophic lake are shaped by different, yet interrelated physicochemical parameters and both groups significantly influence each other. To verify this hypothesis, the species distribution of these groups along the environmental gradients in a eutrophic lake (Lake Góreckie, NW Poland) was investigated. During the study 198 phytoplankton and 26 bacteria taxa were detected in 84 samples. The analysis of their temporal and spatial distribution revealed that phytoplankton and bacteria respond to ecological gradients in a roughly independent manner. Temperature, NH4+ and total phosphorus turned out to be the most important environmental gradients for the phytoplankton, while of concentrations oxidized nitrogen forms (NO3-, NO2-) were more relevant for heterotrophic bacteria. The results obtained in this study allowed us to identify cluster of culturable bacteria connected with high concentrations of chlorophyll a (and thus with the presence of phytoplankton). The group of phytoplankton-associated bacteria includes strains identified as Bacillus megaterium, B. simplex, Paenibacillus amylolyticus, Enterobacter cancerogenus and all the detected members of genus Exiguobacterium. These results are the next step towards an understanding of the microbial food web functioning in eutrophic lakes; an understanding which is crucial in the context of the protection and management of these ecosystems.
    Full-text · Article · Feb 2015 · Carpathian journal of earth and environmental sciences
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    Grzegorz Nowicki · Jakub Barylski · Natalia Kujawa · Anna Goździcka-Józefiak
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    ABSTRACT: The genus Lelliottia was recently created from the group of environmental gammaproteobacteria previously included in the genus Enterobacter. Here, we report the complete genome sequence of phD2B, the first (according to our current knowledge) known phage that infects bacterium from the taxon. Copyright © 2014 Nowicki et al.
    Full-text · Article · Nov 2014 · Genome Announcements
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    ABSTRACT: Recent data demonstrate that human papilloma virus (HPV) plays a role in pathologies other than ano-genital cancers, specifically head and neck malignancies, and non-cancerous conditions such as recurrent respiratory papillomatosis (RRP). High-risk HPV16 and 18, and low risk HPV6 and 11 play the main role in HPV-related pathologies. As more and more information about the role of HPV infection in non-cervical diseases is amassed, additional questions about whether prophylactic HPV vaccines will effectively prevent these conditions are raised. HPV vaccination programs for the cervical pathology are being implemented worldwide. In the United States, the US Food and Drug Administration (FDA) approved the quadrivalent HPV vaccine for girls in 2006 and for boys in 2011. These vaccination programs were aimed at the genital, HPV-related lesions, and there was not much recognition at that time of how HPV vaccination programs might affect oral HPV infection, which is a risk factor for the development of HPV-related head and neck cancers. Vaccination has proved to be a successful policy, and an extant recommendation is aimed at preventing HPV and associated cervical and other anogenital cancers with the routine use of HPV vaccines for males and females. However, HPV vaccines are presently not recommended for preventing oropharyngeal cancer (OPC), although they have been shown to be highly effective against the HPV strains that are most commonly found in the oropharynx. This review is aimed at presenting the evidence-based knowledge concerning HPV vaccination and highlighting the trials and strategies for vaccine administration in HPV-dependent head and neck pathologies.
    Full-text · Article · Jul 2014 · Otolaryngologia polska. The Polish otolaryngology
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    Justyna Broniarczyk · Martina Bergant · Anna Goździcka-Józefiak · Lawrence Banks
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    ABSTRACT: Infection with human papillomaviruses (HPV) requires the minor capsid component L2, which plays an essential role in directing appropriate endosomal trafficking. Previous studies have indicated an infection route involving multi-vesicular bodies (MVBs), and an essential element in their biogenesis is the ESCRT machinery. Here we show that the ESCRT component TSG101 is required for optimal infection with both HPV-16 and BPV-1, with loss of TSG101 resulting in a decrease in viral infection, whereas overexpressed TSG101 increases rates of infection. We find that L2 proteins from multiple PV types interact with TSG101 and show that this interaction contributes to an alteration in the subcellular distribution of L2. In addition, TSG101 can modulate the levels of L2 polyubiquitination. These results demonstrate that TSG101 plays an important part in infection with diverse PVs, and suggests that trafficking of HPV through the ESCRT machinery and MVBs is part of infectious virus entry.
    Preview · Article · Jul 2014 · Virology
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    ABSTRACT: Our previous study demonstrated a decreased expression of tumor susceptibility gene 101 (TSG101) in cervical cancer cells. To identify the mechanism responsible for TSG101 downregulation during cervical cancer development, we analyzed the TSG101 promoter using cis-element cluster finder software. One of the transcription factors whose binding site was detected in the TSG101 promoter was late SV40 factor (LSF). The aim of this study was to analyze the TSG101 protein and LSF expression levels during cervical cancer development. Immunohistochemical analysis confirmed a previously observed decreased expression of TSG101, whereas quantitative polymerase chain reaction (qPCR) and immunohistochemistry analysis revealed high expression of LSF in cervical, precancer and cancer cells compared with human papillomavirus (HPV)-negative non-cancer samples. High expression of LSF in cervical cancer HPV-positive cells suggests that this protein may be important in the regulation of TSG101 expression, as well as in cervical carcinogenesis. The role of LSF as a mediator in cervical cancer development must be confirmed in future studies.
    Full-text · Article · May 2014 · Oncology letters
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    Jakub Barylski · Grzegorz Nowicki · Anna Goździcka-Józefiak
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    ABSTRACT: The Bacillus phage phiAGATE is a novel myovirus isolated from the waters of Lake Góreckie (a eutrophic lake in western Poland). The bacteriophage infects Bacillus pumilus, a bacterium commonly observed in the mentioned reservoir. Analysis of the phiAGATE genome (149844 base pairs) resulted in 204 predicted protein-coding sequences (CDSs), of which 53 could be functionally annotated. Further investigation revealed that the bacteriophage is a member of a previously undescribed cluster of phages (for the purposes of this study we refer to it as "Bastille group") within the Spounavirinae subfamily. Here we demonstrate that these viruses constitute a distinct branch of the Spounavirinae phylogenetic tree, with limited similarity to phages from the Twortlikevirus and Spounalikevirus genera. The classification of phages from the Bastille group into any currently accepted genus proved extremely difficult, prompting concerns about the validity of the present taxonomic arrangement of the subfamily.
    Full-text · Article · Jan 2014 · PLoS ONE
  • Wojciech Kwaśniewski · Józef Kotarski · Grzegorz Polak · Anna Goździcka-Józefiak · Jan Kotarski
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    ABSTRACT: The human insulin-like growth factor (IGF) system has attracted significant researcher interest due to its endocrine and autocrine / paracrine activities, mitogenic effects and the involvement in the regulation of proliferation, differentiation and apoptosis. The signaling pathways used by the IGF system impact cellular metabolism in a complex manner complex and many details are still unclear. Understanding the molecular mechanism of action of IGF's and their effects on cellular activity may provide a basis to develop new anticancer drugs. This review focuses on recent studies that expand our knowledge of the signaling pathways of IGF system.
    No preview · Article · Jan 2014
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    ABSTRACT: Background/aims: The aim of this study was to associate children's growth disorders with polymorphisms detected in the P1 promoter region of IGF1 (including SNP and (CA) n microsatellite repeat polymorphism) and IGF1 and IGFPB3 levels. Methods: IGF-1 gene P1 promoter polymorphism was analyzed in DNA obtained from the blood of 51 children with growth disorders and 50 healthy children without growth disorders by means of PCR-SSCP and sequencing. Results: Among children with growth disorders and the control group we found previously described polymorphisms in the P1 promoter of the IGF-1 gene (rs35767, rs5742612) and different genotypes. The frequency of both detected polymorphisms was no significantly different in the study and the control groups. The CA repeat sequence within the group of children in the study ranged from 11 to 21. The most common were homozygote 19/19 (49.02%) and heterozygote 19/20 (27.45%). Our results did not show any association between polymorphisms in the P1 promoter and IGF-1 levels in the serum of children with growth disorders. Conclusions: This study demonstrated that SNP and (CA) n microsatellite repeat polymorphisms by themselves are not the primary regulatory elements of IGF-1 expression. However, our bioinformatics analysis has shown that the (CA) n microsatellite region in the P1 promoter of IGF-1 is able to form DNA loop structures which can modulate transcription.
    No preview · Article · Jan 2014 · Pediatric endocrinology
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    ABSTRACT: Plant antimicrobial peptides (AMPs) are a component of barrier defense system of plants. They have been isolated from roots, seeds, flowers, stems, and leaves of a wide variety of species and have activities towards phytopathogens, as well as against bacteria pathogenic to humans. Thus, plant AMPs are considered as promising antibiotic compounds with important biotechnological applications. Plant AMPs are grouped into several families and share general features such as positive charge, the presence of disulfide bonds (which stabilize the structure), and the mechanism of action targeting outer membrane structures.
    Full-text · Article · Oct 2013 · Folia Microbiologica
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    Mariusz Skoczyński · Anna Goździcka-Józefiak · Anna Kwaśniewska
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    ABSTRACT: Abstract Objective: The aim of this study was to analyze the relationship between the frequency of Human Papilloma Virus (HPV) isolation in pregnant women and their offspring, and to assess the risk of maternal-neonatal transmission. Study Design: The study included vaginal/buccal smears of 135 pregnant women, as well as the buccal smears and the respiratory discharge samples from their neonates. The material was tested for the presence of HPV DNA by means of PCR. Results: 22 HPV 11-positive cases (16.3%) were detected amongst pregnant women, along with 16 neonatal HPV 11-positive cases (11.85%). The concordance rate of maternal and neonatal HPV 11 DNA was 54.5%. Three maternal variables: the presence of HPV 11, less than 10 years of education, and common law proved significant risk factors for vertical transmission. Conclusion: Asymptomatic HPV infection of a pregnant woman rather than the mode of delivery or other obstetrical characteristics constitutes significant risk factor of vertical transmission.
    Full-text · Article · May 2013 · The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
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    Robert Nawrot · Łukasz Tomaszewski · Anna Czerwoniec · Anna Goździcka-Józefiak
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    ABSTRACT: The family of glycine-rich plant proteins (GRPs) is a large and complex group of proteins that share, as a common feature, the presence of glycine-rich domains arranged in (Gly)n-X repeats that are suggested to be involved in protein–protein interactions, RNA binding, and nucleolar targeting. These proteins are implicated in several independent physiological processes. Some are components of cell walls of many higher plants, while others are involved in molecular responses to environmental stress, and mediated by post-transcriptional regulatory mechanisms. The goals of this study are to identify the coding sequence of a novel glycine-rich RNA-binding protein from Chelidonium majus and to propose its structural model. DNA fragments obtained using degenerate PCR primers showed high sequence identities with glycine-rich RNA-binding protein coding sequences from different plant species. A 439-bp nucleotide sequence is identified coding for a novel polypeptide composed of 146 amino acids, designated as CmGRP1 (C. majus glycine-rich protein 1), with a calculated MW of 14,931 Da (NCBI GenBank accession no. HM173636). Using NCBI CDD and GeneSilico MetaServer, a single conserved domain, the RNA recognition motif (RRM), was detected in CmGRP1. The C-terminal region of CmGRP1 is a glycine-rich motif (GGGGxxGxGGGxxG), and it is predicted to be disordered. Based on a 1fxl crystal structure, a 3D model of CmGRP1 is proposed. CmGRP1 can be classified as a class IVa plant GRP, implicated to play a role in plant defense.
    Full-text · Article · Apr 2013 · Plant Molecular Biology Reporter
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    J Durzyńska · A Wardziński · M Koczorowska · A Goździcka-Józefiak · E R Barton
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    ABSTRACT: Several physiological activities have been assigned to E-peptides derived from pre-pro-insulin-like growth factor (IGF1) processing; however, the whole range of the E-peptides' functions is still unknown. The objective of this study was to investigate human Eb peptide (hEb) in terms of its bioactivity, cellular localization, and intracellular trafficking using human cancer cells. Human Eb fused with red fluorescence protein (RFP) or green fluorescence protein (GFP) localizes strongly to nucleoli and to a lesser extent to nuclei of HeLa and U2-OS cells. Mutagenesis of hEb nucleolus localization sequence (NoLS) leads to its partial delocalization from nuclei and nucleoli to cytoplasm of transfected cells. Thus, NoLS is not sufficient for the hEb to be localized in nucleoli of the cells and a different mechanism may be involved in hEb targeting. A BrdU ELISA showed that the proliferation index of cells expressing hEb hybrid proteins increased up to 28%. For comparison, the same assay was performed using HeLa cells treated extracellularly with synthetic hEb. A significant increase in the proliferation index was observed (41-58% for concentrations ranging from 10-100 nM, respectively). Additionally, a cell migration assay was performed using stable U2-OS cell lines expressing hEb fused with RFP or RFP alone as a negative control. The migration index of hEb expressing cells was 38.3% greater. The increase in cell proliferation index and in motile properties of hEb expressing cells demonstrate that hEb is more than a pre-pro-IGF1b processing product, and has intrinsic activity of biological significance.
    Full-text · Article · Jan 2013 · Hormone and Metabolic Research
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    ABSTRACT: insulin-like growth factor-I (IGF-I) is one of the proteins participating in the transfer of growth signals, which are responsible in certain cases for the etiology of a growth disorder. Aim of the study: The aim of the study was an analysis of the coding sequence of the extracellular and intracellular domains of IGF-IR responsible for ligand binding (IGF-I) and kinase activity in the DNA of children with growth disorders, who have normal or slightly decreased levels of plasma IGF-I. Material and methods: DNA isolated from the peripheral blood of 50 short-statured children was used as study material. DNA fragments of IGF-IR obtained as a result of PCR amplification were analyzed using single stranded conformation polymorphism (SSCP) and sequencing. Results: We did not observe any changes in the IGF-IR sequences, thus it can be excluded as a factor responsible for growth disorders. Conclusions: IGF-I receptor sequence changes are not the cause of growth disorders in the study group of children. To find the cause of growth disorders in the study group other proteins from somatotropic axis and/or signaling pathways should be studied in the future.
    Full-text · Article · Jan 2013 · Endokrynologia, diabetologia i choroby przemiany materii wieku rozwojowego: organ Polskiego Towarzystwa Endokrynologow Dzieciecych
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    ABSTRACT: To assess the prevalence of human papilloma virus (HPV) in esophageal squamous cell carcinoma (ESCC) in the south-eastern region of Poland. The study population consisted of 56 ESCC patients and 35 controls. The controls were patients referred to our department due to other non-esophageal and non-oncological disorders with no gross or microscopic esophageal pathology as confirmed by endoscopy and histopathology. In the ESCC patients, samples were taken from normal mucosa (56 mucosa samples) and from the tumor (56 tumor samples). Tissue samples from the controls were taken from normal mucosa of the middle esophagus (35 control samples). Quantitative determination of DNA was carried out using a spectrophotometric method. Genomic DNA was isolated using the QIAamp DNA Midi Kit. HPV infection was identified following PCR amplification of the HPV gene sequence, using primers MY09 and MY11 complementary to the genome sequence of at least 33 types of HPV. The sequencing results were computationally analyzed using the basic local alignment search tool database. In tumor samples, HPV DNA was identified in 28 of 56 patients (50%). High risk HPV phenotypes (16 or/and 18) were found in 5 of 56 patients (8.9%), low risk in 19 of 56 patients (33.9%) and other types of HPV (37, 81, 97, CP6108) in 4 of 56 patients (7.1%). In mucosa samples, HPV DNA was isolated in 21 of 56 patients (37.5%). High risk HPV DNA was confirmed in 3 of 56 patients (5.3%), low risk HPV DNA in 12 of 56 patients (21.4%), and other types of HPV in 6 of 56 patients (10.7%). In control samples, HPV DNA was identified in 4 of 35 patients (11.4%) with no high risk HPV. The occurrence of HPV in ESCC patients was significantly higher than in the controls [28 of 56 (50%) vs 4 of 35 (11.4%), P < 0.001]. In esophageal cancer patients, both in tumor and mucosa samples, the predominant HPV phenotypes were low risk HPV, isolated 4 times more frequently than high risk phenotypes [19 of 56 (33.9%) vs 5 of 56 (8.9%), P < 0.001]. A higher prevalence of HPV was identified in female patients (71.4% vs 46.9%). Accordingly, the high risk phenotypes were isolated more frequently in female patients and this difference reached statistical significance [3 of 7 (42.9%) vs 2 of 49 (4.1%), P < 0.05]. Of the pathological characteristics, only an infiltrative pattern of macroscopic tumor type significantly correlated with the presence of HPV DNA in ESCC samples [20 of 27 (74.1%) vs 8 of 29 (27.6%) for ulcerative or protruding macroscopic type, P < 0.05]. The occurrence of total HPV DNA and both HPV high or low risk phenotypes did not significantly differ with regard to particular grades of cellular differentiation, phases in depth of tumor infiltration, grades of nodal involvement and stages of tumor progression. Low risk HPV phenotypes could be one of the co-activators or/and co-carcinogens in complex, progressive, multifactorial and multistep esophageal carcinogenesis.
    Preview · Article · Oct 2012 · World Journal of Gastroenterology

Publication Stats

307 Citations
72.97 Total Impact Points

Institutions

  • 2000-2015
    • Adam Mickiewicz University
      • • Department of Molecular Virology
      • • Institute of Experimental Biology
      • • Department of Molecular and Cellular Biology
      • • Institute of Molecular Biology and Biotechnology
      Posen, Greater Poland Voivodeship, Poland
  • 2005
    • Institute of Plant Protection - National Research Institute
      • Institute of Plant Protection
      Posen, Greater Poland Voivodeship, Poland
  • 2003-2004
    • Poznan University of Medical Sciences
      • Department of Pediatrics I
      Posen, Greater Poland Voivodeship, Poland
  • 1997-2001
    • Poznan University of Economics
      Posen, Greater Poland Voivodeship, Poland