A Kasprowicz

Azienda Ospedaliera Santa Maria Nuova di Reggio Emilia, Reggio nell'Emilia, Emilia-Romagna, Italy

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Publications (56)48.54 Total impact

  • Olaf Zagolski · Pawel Strek · Andrzej Kasprowicz · Anna Bialecka
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    ABSTRACT: Background: Polyvalent bacterial lysate (PBL) is an oral immunostimulating vaccine consisting of bacterial standardized ly-sates obtained by lysis of different strains of bacteria. Autovaccines are individually prepared based on the results of smears obtained from the patient. Both types of vaccine can be used to treat an ongoing chronic infection. This study sought to determine which method is more effective against nasal colonization by potential respiratory tract pathogens. Material/Methods: We enrolled 150 patients with aerobic Gram stain culture and count results indicating bacterial colonization of the nose and/or throat by potential pathogens. The participants were randomly assigned to each of the following groups: 1. administration of PBL, 2. administration of autovaccine, and 3. no intervention (controls). Results: Reduction of the bacterial count in Streptococcus pneumoniae-colonized participants was significant after the autovaccine (p<0.001) and PBL (p<0.01). Reduction of the bacterial count of other beta-hemolytic streptococcal strains after treatment with the autovaccine was significant (p<0.01) and was non-significant after PBL. In Haemophilus influenzae colonization, significant reduction in the bacterial count was noted in the PBL group (p<0.01). Methicillin-resistant Staphylococcus aureus colonization did not respond to either treatment. Conclusions: The autovaccine is more effective than PBL for reducing bacterial count of Streptococcus pneumoniae and beta-he-molytic streptococci, while PBL was more effective against Haemophilus influenzae colonization.
    No preview · Article · Oct 2015 · Medical science monitor: international medical journal of experimental and clinical research
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    ABSTRACT: Staphylococcus aureus strains were isolated from mastitic milk of cows with infected mammary glands. The animals were living in 12 different farms near Lublin, in Central-Eastern Poland. A biochemical identification method based on enzymatic assay was performed, followed by haemolytic and proteolytic tests. PCR-RFLP targeted on the gap gene allowed the genetic identification of strains at the species level and verified phenotypic identification results. A molecular typing method using triplex PCR was performed to recognize the genetic similarity of the analyzed strains. DNA microarray hybridization (StaphyType, Alere Technologies) was used for detection of antibiotic resistance and virulence associated markers. The results obtained indicate high genetic similarity in strains isolated from the same sites. High genetic similarities were also detected between strains isolated from cows from different farms of the same region. A slightly lower similarity was noted however, in strains from various regions indicating that the strains are herd specific and that the cow's infections caused by S. aureus were of a clonal character. In 21 representative isolates selected for DNA-microarray testing, only fosfomycin (fosB) and penicillin resistance markers (blaZ, blaI, blaR) were detected. The presence of genes coding for haemolysins (lukF, lukS, hlgA, hla, hld, hlb), proteases (aur, sspA, sspB, sspP), enterotoxins (entA, entD, entG, entI, entJ, entM, entN, entO, entR, entU, egc-cluster), adhesins (icaA, icaC, icaD, bbp, clfA, clfB, fib, fnbA, map, vwb) or immune evasion proteins (scn, chp, sak) was common and, with exceptions, matched triplex PCR-defined clusters.
    Full-text · Article · Jul 2015 · Polish journal of veterinary sciences

  • No preview · Article · Apr 2015 · Advances in Experimental Medicine and Biology
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    ABSTRACT: Staphylococcus pseudintermedius is a veterinary pathogen that has seldom been described as an agent of human disease. Features of this probably underreported coagulase-positive Staphylococcus species are depicted here through the description of a graft-versus-host disease-related wound infection caused by a multidrug-resistant strain.
    Full-text · Article · Mar 2013 · Journal of clinical microbiology
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    Full-text · Article · Jan 2013 · Journal of Clinical Microbiology
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    ABSTRACT: Antibiotic resistance is a common problem accompanying biofilm-associated chronic infections. New therapeutic strategies are based on a combined application of antiseptics with anti-biofilm agents. Taurine haloamines, taurine chloramine (TauCl) and taurine bromamine (TauBr), show antimicrobial and anti-inflammatory properties, which have been examined in a variety of local infections, including biofilm-associated infections. In contrast to beneficial antimicrobial effects of taurine haloamines against the planktonic form of bacteria, their efficacy against bacteria hidden in biofilm need to be enhanced. One possibility is to use them together with agents capable of destroying components of biofilm matrix. In this study we ask a question whether TauCl or TauBr are effective in killing Streptococcus mutans and Porphyromonas gingivalis, major oral bacteria responsible for the development of dental plaque and pathogenesis of periodontal diseases. Moreover, we have examined TauBr and TauCl stability in the presence of N-acetylcysteine (NA C) and DNase, agents with known anti-biofilm activity. We have found that TauBr was much stronger than TauCl microbicidal agent against both tested bacterial strains. However, TauBr was less stable than TauCl. NAG readily decomposed TauBr but not TauCl. In addition, TauBr inhibited DNase activity, when used in excess. This preliminary study confirms previous opinions that taurine haloamines have great potential in killing oral bacteria. However, further studies are necessary to find anti-biofilm agent(s) which together with TauCl/TauBr will give at least an additive therapeutic effect in the treatment of chronic infections, to support or replace ineffective antibiotic therapy.
    No preview · Article · Jan 2013 · Central-European Journal of Immunology
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    Full-text · Article · Jul 2012 · Veterinary Microbiology
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    ABSTRACT: Propionibacterium acnes is dominating group of resident bacteria in skin biocenosis. These bacteria participate in autosterilisation of skin with the process of decomposition of triglycerides into free fatty acids and by keeping the pH of skin on the level 5.5. When the process goes out of control the excess of fatty acids in sebaceous glands leads to necrosis and inflammation. Apart of the presence on the skin P acnes also are present in mucous membranes of intestinal tract, eyes, internal ears channels, and in upper respiratory tracts. Traditionally they are regarded as anaerobes, but they tolerate oxygen atmosphere and are resistant to phagocytosis, surviving even in macrophages. These bacteria produce a number of enzymes and proinflammatory factors activating monocytes, stimulating mitogenic activity of lymphocytes T. According to common opinion they are responsible for disease acne vulgaris, but there are also researchers claiming their low pathogenicity. The list of the P acnes related diseases is not short, some of these diseases may be facilitated by predisposing factors as surgery interventions, diagnostic, or cosmetic procedures. The aim of the study was to compare standard biochemical analysis of P acnes strains to genotypic typing basing on the results from MP-PCR analyses. Relations of hemolysis activity to biochemical types or genetic types were also analysed. The analysis of 66 P acnes strains isolated from skin lesions of patients with acne vulgaris was performed. A collection of the strains was analysed biochemically according to Pulverer, Sourek and Hoffler method modified by Kasprowicz, and typed genetically by MP-PCR method. Relations of biochemical and genetic types to beta-hemolysis of strains were studied. Dominating biochemical type was type number I grouping 79% of all isolates, and dominant genotype was A which was detected in 75% of all collected strains. Beta-hemolysis was a feature present in 34% of strains, more frequently in type I (40%) than in type II (12%). beta-hemolysis was present only in A-genotype strains, but A-genotype by itself does not determine that reaction: 53% of A-genotype strains does not exhibit beta-hemolysis. All type I strains represent A-genotype. Type II was genotypicaly differentiated: all genotypes A, A', B, and C were present. The results obtained show genotypic heterogenity of P acnes strains and relations to biochemical types. Hemolysis was detected independently to biotype or genotype representation. The results confirm biochemical and genetic heterogenities of P acnes, but the observations also indicate necessity of further microbiological-molecular investigation of that bacteria group using other molecular techniques to the study.
    Full-text · Article · Jan 2012 · Medycyna doświadczalna i mikrobiologia
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    ABSTRACT: To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. A total number of 104 isolates representing different staphylococcal species were isolated and identified using routine biochemical methods applied in diagnostic laboratories. Among 17 isolated staphylococcal species, Staphylococcus intermedius was the most common species isolated from both healthy or diseased dogs living either in animal shelter or household environments. The pattern of Staphylococcus sp. colonization differs considerably for animals living in the two tested habitats. In particular, S. aureus MRSA and MSSA isolates were detected only in infected skin lesion samples from animals that dwelled in the animal shelter. As could be expected, S. intermedius was found to be a predominant causative agent in canine skin infections. In our study, we demonstrated that S. intermedius in its carrier-state, inhabits mainly the mucosal membrane of the nasal vestibule. It was also found in the samples taken from the skin, the lumbo-sacralis triangle and perineum, but was rarely isolated from the ears.
    Preview · Article · Jan 2011 · Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
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    ABSTRACT: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
    No preview · Article · Jan 2010 · ChemInform
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    ABSTRACT: We have reported a bacterial infection in a dog with progressive dysplasia of the hips. Orthopedic surgery was performed. Seven weeks prior to the surgery, the patient was bitten by another dog. The postimplantation wound exuded for four days after the surgery. Microbiological analysis performed by standard identification techniques showed the presence of Staphylococcus intermedius, but an additional molecular analysis indicated S. pseudintermedius. This was followed by an evaluation of antibiotic susceptibility of the strain which showed cefoxitin, ciprofloxacin, clindamycin, trimethoprim/sulfamethoxazole, doksycycline, erythromycin, and gentamicin resistance. Minimal inhibitory concentration (MIC) values for selected antibiotics were reported. Resistance for cefoxitin indicates that methicillin resistant S. pseudintermedius (MRSP) strains were present in individual macroorganisms, but they can expand and persist the colonization of other hosts.
    Full-text · Article · Jan 2010 · Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
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    ABSTRACT: Taurine bromamine (TauBr), the product of taurine and hypobromous acid (HOBr), exerts anti-inflammatory and antibacterial properties. Recently we have shown that Propionibacterium acnes, a potential pathogenic agent of acne, is extremely sensitive to TauBr. As topical antibiotics are associated with the emergence of resistant bacteria, TauBr seems to be a good candidate for topical therapy for acne vulgaris. In our double blind investigation, the efficacy and safety of 3.5 mM TauBr cream was evaluated. 1% Clindamycin gel (Clindacin T), one of the most common topical agents in the treatment of acne vulgaris, was used as a control. Forty patients with mild to moderate inflammatory facial acne vulgaris were randomly treated with either TauBr or clindamycin for 6 weeks, twice-a-day. More than 80% of the patients markedly improved with both treatments, without any adverse effects observed. Both TauBr and clindamycin produced a significant reduction in inflammatory skin lesion counts (papules/ pustules). After 6 weeks, comparable reductions of acne lesions, 65% and 68%, were observed in the TauBr and clindamycin groups, respectively. In conclusion, these data support our concept that TauBr can be used as a topical agent in the treatment of acne vulgaris, especially in patients who have already developed antibiotic resistance.
    No preview · Article · Jul 2008 · European journal of dermatology: EJD
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    ABSTRACT: Typing of Staphylococcus aureus isolates is a necessary procedure for monitoring the transmission of S. aureus among carriers and in epidemiology. Evaluation of the range of relationship among isolates rely on epidemiological markers and is possible because of the clonal character of S. aureus species. Effective typing shows the scheme of transmission of infection in a selected area, enables identifying the reservoir of the microorganism, and may enhance effective eradication. A set of typing methods for use in analyses of epidemiological correlations and the identification of S. aureus isolates is presented. The following methods of typing are described: biotyping, serotyping, antibiogram, protein electrophoresis, cell protein profiles (proteom), immunoblotting, multilocus enzyme electrophoresis (MLEE), zymotyping, and standard species identification of S. aureus in the diagnostic laboratory. Phenotyping methods for S. aureus isolates used in the past and today in epidemiological investigations and in analyses of correlations among S. aureus isolates are presented in this review. The presented methods use morphological characteristics, physiological properties, and chemical structures of the bacteria as criteria for typing. The precision of these standard methods is not always satisfactory as S. aureus strains with atypical biochemical characters have evolved recently. Therefore it is essential to introduce additional typing procedures using molecular biology methods without neglecting phenotypic methods.
    No preview · Article · Feb 2008 · Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine)
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    ABSTRACT: Taurine chloramine (TauCl) and taurine bromamine (TauBr) are the main haloamines produced by activated neutrophils. TauCl exerts both anti-inflammatory and microbicidal activities. Clinical studies showed that TauCl may be useful as an antimicrobial agent in the local treatment of infections. Much less is known about TauBr. Circumstantial evidence suggests that Propionibacterium acnes (PA) has a role in the inflammation of acne. Available topical therapies include antimicrobial agents which reduce total PA numbers and anti-inflammatory agents which suppress activity of the cells present in acne inflammatory lesions. In this study the bactericidal activities of TauBr and TauCl against PA and Staphylococcus epidermidis (SE), as a control strain, were investigated. Moreover, the influence of these haloamines on the generation of reactive oxygen species (ROS) by activated neutrophils was also tested. TauBr and TauCl were prepared by reaction of taurine with HOBr and HOCl, respectively. The reaction was monitored by UV absorption spectra. The bactericidal activities of TauBr and TauCl were determined by the pourplate method. The generation of ROS by neutrophils was determined by luminol chemiluminescence assay. In our experimental set-up, TauBr showed stronger antibacterial activity than TauCl. Interestingly, PA was significantly more susceptible to TauBr than SE was. Moreover, TauBr at non-cytotoxic concentrations significantly reduced ROS generation by neutrophils. Since PA is considered to be an etiological agent in acne and ROS are closely correlated with the pathogenesis of inflammatory skin diseases, the reported data suggest that TauBr may be a good candidate for the topical therapy for acne vulgaris.
    No preview · Article · Feb 2006 · Archivum Immunologiae et Therapiae Experimentalis
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    ABSTRACT: Staphylococcus aureus has important implications for the pathogenesis of atopic dermatitis (AD). In some patients S. aureus can be eradicated from the skin during anti-inflammatory treatment, while in others bacterial colonization is persistent. Potential mechanisms and features of these two distinct groups of patients are not known. Accordingly, we studied relationships between the ability to eliminate S. aureus during an anti-inflammatory treatment and selected clinical and immunological features. Quantitative assessment of S. aureus on the skin, in nasal vestibule and throat, serum IgE levels, CD4/CD8 T-cell ratio, lymphocyte proliferation and phagocyte oxidative burst were determined during the exacerbation and after 4 and 12 weeks of the treatment using topical steroid and oral antihistamine in 34 patients with AD. S. aureus was found on the skin of all 34 patients during exacerbation. Disease severity scoring of atopic dermatitis (SCORAD) correlated with the density of bacteria. Treatment with oral antihistamine and topical steroid resulted in a significant alleviation of symptoms, which correlated with the elimination of S. aureus from the skin in 70% of patients. In the remaining 30% of patients, dense (more than 10(10)/cm2) S. aureus skin colonization, persisted despite the treatment. Patients with persistent S. aureus presented with higher serum IgE levels, lower lymphocyte proliferation in response to staphylococcal enterotoxin B, phytohaemagluttinin and anti-CD3. Persistence of S. aureus was more common in men. Patients with AD differ in the ability to clear S. aureus from the skin during anti-inflammatory treatment, which appears to be related to the abnormalities in immunological parameters. Local antibiotic therapy should be considered only in patients with persistent S. aureus colonization.
    No preview · Article · May 2005 · Clinical & Experimental Allergy
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    ABSTRACT: Propionibacterium acnes (PA) and Staphyloccocus epidermidis (SE) are two major bacterial strains isolated from acne lesions. Nevertheless, only PA seems to be implicated in the pathogenesis of inflammatory acne vulgaris. Evidence for this, however, remains indirect and the precise role of PA in inflammatory acne is still a matter for conjecture. The aim of this study was to compare some pro-inflammatory and adjuvant properties of PA and SE. To determine some of the pathogenic, immunostimulatory, and pro-inflammatory proper of PA and SE, two experimental models of inflammation were used. In vivo; chronic inflammation was induced by intradermal injection of living bacteria into the ear. In vitro; peritoneal macrophages elicited by the bacteria were examined for their ability to generate reactive oxygen species (ROS), nitric oxide (NO), and cytokines. PA, but not SE, evoked mild local inflammation of infected ears. Macrophages elicited with PA produced more tumor necrosis factor alpha and interleukin IL-12 than those induced with SE, while SE was a stronger inducer of IL-10 production. Both bacteria equally induced the generation of NO and ROS. In contrast, only PA showed adjuvant proper-ties. The results of these studies indicate that SE, in contrast to PA, does not exert pro-inflammatory properties. Thus it is unlikely that SE may be implicated in the pathogenesis of inflammatory acne vulgaris.
    No preview · Article · Jan 2005 · Archivum Immunologiae et Therapiae Experimentalis
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    ABSTRACT: Propionibacterium acnes is a component of physiological flora of human skin. It colonizes the outlets of sebaceous glands and participates in the pathogenesis of inflammatory acne. Acne vulgaris is a common skin disease. It is found in more or less exacerbated form in approximately 85% of adolescent population. The main purpose of the research was to confirm the hypothesis of Propionibacterium bacteria participation in the aetiopathogenesis of acne vulgaris. The researches have proved the presence of Propionibacterium acnes on the surface of the skin both of people with acne-related changes and these with whom such changes were not found. Statistically significant differences were found in the number of P. acnes bacteria per 1 square centimeter of healthy and disease-affected skin as well as in the diversity of biochemical types. The highest number of P. acnes bacteria have been found in fresh changes with visible symptoms of inflammation. In order to confirm the hypothesis of the participation of Propionibacterium bacteria in the aetiopathogenesis of acne, a detailed phenotypical analysis of isolated P. acnes strains have been conducted. Type, biotype, resistance pattern, proteolytic and lipolytic properties have been determined.
    No preview · Article · Feb 2004 · Medycyna doświadczalna i mikrobiologia
  • J Miedzobrodzki · P Kaszycki · A Bialecka · A Kasprowicz
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    ABSTRACT: Staphylococcus aureus strains isolated from the colonized skin lesions of 26 patients with acute-phase atopic dermatitis were reported to produce various extracellular proteolytic enzymes. Using the skim-milk-agar culture plating method, it was shown that 97% of the strains (65 of 67 examined) produced proteolytic activity, with 61% (42 strains) producing activity comparable to that of the proteolytically hyperactive reference strain Staphylococcus aureus V8. This observation was confirmed by azocasein degradation with culture supernatants, which indicated that 91% of the strains produced extracellular proteinases and 43% exceeded the 2% activity threshold of the reference strain. Control strains were isolated from the nose vestibules of 18 healthy carriers; the proteolytic activity of these strains never exceeded 2.5% of the activity of the reference strain. In 54% of the patients examined ( n=14), the activity of the strains was higher than that determined for the isolates from the control group. The combined use of assays incorporating azocasein and a synthetic chromogenic substrate, N-CBZ-Phe-Leu-Glu- pNA, showed that two staphylococcal enzymes, Staphylococcus aureus metalloproteinase (SAMP) and Staphylococcus aureus serine proteinase (SASP), contributed to the total proteolytic activity released by the strains examined. The contribution of each of the two enzymes varied greatly between different isolates. The undamaged skin of the patients was not colonized with Staphylococcus aureus. The presence of several strains with atypical proteinase characteristics was also reported, suggesting the possible involvement of enzymes other than serine- and metallo-proteinases in the proteolytic activity of Staphylococcus aureus. Taken together, the results of the study imply that staphylococcal proteinases may contribute to the pathogenicity of atopic dermatitis.
    No preview · Article · May 2002 · European Journal of Clinical Microbiology
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    ABSTRACT: Various 5-chloroarylidene-2-amino substituted derivatives of imidazoline-4-one were synthesized and evaluated for their activity in vitro against Mycobacterium tuberculosis and other type strains of bacteria and fungi. 2-Chloro- and 2,4-dichlorobenzylidene substituted hydantoins exhibited antimycobacterial effect. The most potent compounds 3i, 3j, 3o, 3q and 3s were classified for further tests. The antimitotic effect of the investigated hydantoins was also examined.
    No preview · Article · Feb 2002 · Il Farmaco
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    ABSTRACT: Atopic dermatitis (AD) is a complex inflammatory skin disorder of unclear pathogenesis. Skin colonization with Staphylococcus aureus (S. aureus) may play an important role in the pathogenesis of AD due to its allergenic, as well as superantigenic properties. The aim of the present study was to analyze the effects of oral antihistamine and topical corticosteroids treatment on the presence and intensity of skin colonization with S. aureus in relation to the skin lesion intensity in AD. The study included 34 patients with long-duration atopic dermatitis during exacerbation of the disease and after 4 and 12 weeks of the treatment. At these timepoints bacteriological and clinical studies were conducted and total scrum IgE levels were measured. S. aureus skin colonization was found in all (100%) subjects during exacerbation of the disease, and its intensity was significantly correlated with the clinical severity of skin lesions and with IgE levels. In 70% of patients the treatment caused elimination of S. aureus from the skin of patients. In the remaining group (30%) the S. aureus colonization persisted during the treatment. This group was characterized by incomplete remission and significantly higher IgE levels than the remaining subjects eliminated S. aureus colonization. Additionally, the persistence of S. aureus colonization was associated with coexistence of inhalant allergen hypersensitivity. In conclusion, skin colonization with S. aureus plays important role in the development and persistence of skin lesions in AD patients. Patients who do not eliminate S. aureus during classical treatment with antihistamines and topical steroids may require additional antibiotic treatment.
    No preview · Article · Jan 2002 · Alergia Astma Immunologia

Publication Stats

326 Citations
48.54 Total Impact Points

Institutions

  • 2012
    • Azienda Ospedaliera Santa Maria Nuova di Reggio Emilia
      Reggio nell'Emilia, Emilia-Romagna, Italy
  • 2010
    • Jagiellonian University
      • Department of Chemical Technology
      Cracovia, Lesser Poland Voivodeship, Poland
  • 2005
    • Polish Academy of Sciences
      Warszawa, Masovian Voivodeship, Poland
  • 1977
    • University of Cologne
      Köln, North Rhine-Westphalia, Germany