[Show abstract][Hide abstract] ABSTRACT: Mycobacterium heraklionense sp. nov. (M. heraklionense) is a novel non-tuberculous mycobacterium belonging to the Mycobacterium terrae complex that has recently been described. It has a world-wide distribution. Recently, a case of tenosynovitis in an immunocompetent individual caused by M. heraklionense was reported, indicating that it has the ability to cause diseases. In the present study, in order to provide a more detailed profile of this mycobacterium and to obtain a more complete overall picture of its clinical significance, we report all available data regarding the initial 12 cases of its isolation. Of the 12 patients, 5 (42%) eventually died within a period of 3 months following the isolation of the mycobacterium. However, any connection between the presence of M. heraklionense and these deaths could not be documented. These 5 patients were all males with a mean age of 74.6 years suffering from serious underlying diseases, which most probably were the cause of death. Additional data from possible new cases of M. heraklionense isolation are anticipated.
Preview · Article · Oct 2015 · Experimental and therapeutic medicine
[Show abstract][Hide abstract] ABSTRACT: The aim of the present study was to evaluate CD105 tissue marker in the bone marrow (BM) of multiple myeloma (MM) patients. CD105 was evaluated using immunohistochemical method. An effort was made to correlate this marker with BM microvascular density (MVD) along with other known markers of angiogenesis in order to evaluate its clinical significance.
BM MVD was estimated by CD31. CD105 in BM was estimated by immunohistochemical method in 54 newly diagnosed patients with MM. Circulating levels of known angiogenic factors such as basic fibroblast growth factor (b-FGF) and soluble CD105 (sCD105) were measured by ELISA in the same group of patients. All these factors were also measured in 20 age- and sex-matched healthy controls.
We found that CD105 MVD, along with the expected CD31 MVD, and serum levels of sCD105 and bFGF were increased, also in parallel with disease stage, and all were decreased after effective treatment. Moreover, CD105 MVD correlated with all the aforementioned markers of angiogenesis.
Our results indicated that CD105 MVD is following the behavior of CD31 MVD in MM, suggesting being a valid marker of BM neoangiogenesis in MM. Its prognostic impact remains to be proven.
No preview · Article · Mar 2015 · Journal of Cancer Research and Clinical Oncology
[Show abstract][Hide abstract] ABSTRACT: In multiple myeloma the angiogenic process is enhanced by various mediators. Among them interleukin-10 (IL-10), secreted mainly by myeloma-associated macrophages seems to participate in myeloma progression with variable manners. The aim of the study was to measure serum levels of IL-10 in various stages of MM patients and to correlate them with various angiogenic cytokines, such as vascular endothelial growth factor and angiopoietin-2 and with known proliferation parameters, such as serum levels of B-cell activating factor and bone marrow infiltration by myeloma plasma cells, in order to explore their clinical significance. We measured serum levels of the above parameters by ELISA in 54 newly diagnosed MM patients. All of them were higher in MM patients and were increasing in parallel with disease progression. Furthermore, IL-10 correlated positively with both angiogenic cytokines and proliferation markers. This correlation of IL-10 with both angiogenic cytokines and markers of disease activity implicates that they all have an important role in MM pathogenesis and progression.
No preview · Article · Mar 2015 · Pathology & Oncology Research
[Show abstract][Hide abstract] ABSTRACT: Intravesical instillation of Bacillus Calmette-Guerin (BCG) is the treatment of choice for superficial bladder carcinoma. Disseminated BCG infection presenting as granulomatous hepatitis or pneumonitis is a very rare complication of this treatment. Here we report a case series of seven patients previously treated with BCG presenting with pneumonitis. In two of the cases, identification of Mycobacterium bovis was achieved with molecular methods.
No preview · Article · Oct 2014 · Indian Journal of Medical Microbiology
[Show abstract][Hide abstract] ABSTRACT: Objective:
Interleukin-22 (IL-22) is a cytokine participating in many aspects of inflammation. Multiple myeloma (MM) is a malignant disease of plasma cells with characteristic immune deregulation. We estimated serum levels of IL-22 in MM patients, both in activity and remission, in order to apprehend its possible participation in MM biology.
We measured serum levels of IL-22 along with beta-2 microglobulin (B2M), paraprotein, and interleukin-1beta (IL-1beta), as well as degree of bone marrow infiltration, in 51 patients with active MM and in 22 of them in remission.
We found that IL-22 was higher in active MM patients, compared to both controls and patients in remission, and also in patients in remission compared to controls. Moreover, IL-22 was increasing in parallel with the disease stage and also correlated with B2M, IL1-beta, and degree of infiltration.
We suggest that the elevated levels of IL-22 in active MM patients, in parallel with disease activity, and in positive correlation with IL-1beta, may represent the inflammatory element of the disease. This increased occurrence of IL-22 may enhance myeloma proliferation and growth, and moreover, may participate in the mechanisms of immune deregulation.
[Show abstract][Hide abstract] ABSTRACT: Background
Fusarium spp. can cause disseminated infections, particularly in immunocompromised patients. Fusarium verticilliodes is a human pathogen, and sporadic cases of fusariosis have been reported.
To report a nosocomial cluster of F. verticilliodes bloodstream infections among seven immunocompetent inpatients following reconstruction works.
Identification was performed using macroscopic and microscopic morphology, and molecular assays (sequencing the nuclear ribosomal internal transcribed spacer region and translation elongation factor-1α gene). Susceptibility testing was performed in accordance with the guidelines of the Clinical and Laboratory Standards Institute. Environmental surveillance specimens were taken and cultured on Sabouraud dextrose agar plates.
In total, 16 blood cultures obtained from the seven patients were positive for F. verticillioides. All surveillance cultures were negative.
In order to prevent fungaemia, it is important to implement effective infection control measures, before, during and after demolition and construction activities in healthcare settings.
Full-text · Article · Apr 2014 · The Journal of hospital infection
[Show abstract][Hide abstract] ABSTRACT: Minocycline is an old, safe, second-line antimicrobial agent that has drawn attention over the last few years as a possible therapeutic option against multidrug-resistant Acinetobacter baumannii (MDRAB) clinical isolates. Recent in vitro and in vivo results indicate that minocycline is a valid, alternative treatment option for minocycline-susceptible MDRAB. Although effective alone, its administration as monotherapy should be avoided. Combinations with other antimicrobials can reduce the MIC of each component, present synergism and minimize the risk for drug resistance. Owing to its limited solubility in urine, it should be avoided for urinary pathogens. The present article reports all available information regarding its use as a therapeutic option against MDRAB.
Full-text · Article · Mar 2014 · Future Microbiology
[Show abstract][Hide abstract] ABSTRACT: A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs.) within the species known to belong to the complex. In the species Mycobacterium arupense were detected 3 sqvs. in the 16S rRNA gene, 6 sqvs. in the hsp65 gene and 15 sqvs. in the rpoB gene; in Mycobacterium senuense 2 sqvs. were present in each of the three genetic regions; in Mycobacterium kumamotonense 4, 2 and 10 sqvs. respectively and in M. terrae 3, 4 and 6 sqvs. respectively were found. The extraneousness of Mycobacterium triviale with the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acids analyses for the differentiation of the members of M. terrae complex was confirmed as well. The survey allowed furthermore to recognize three previously undescribed species characterized by unique sequences in 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov., a species proposed 40 years ago but never validly published, is characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it is related to Mycobacterium hiberniae. The type strains of this species, of which eight strains were investigated, is ATCC27353=DSM45694 A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov. which has intermediate growth rate and is unpigmented, the nitrate reductase activity is typically strong. Closely related to M. arupense in the 16S rRNA gene, M. heraklionense is clearly differentiable from the latter species in the other genetic regions. The type strain is NCTC13432=LMG2473= CECT7509. Mycobacterium longobardum sp. nov., represented in the study from seven strains, is characterized by a unique phylogenetic location within the M. terrae complex, evidently divergent from any other species. The type strain is DSM45394=CCUG58460.
Full-text · Article · Mar 2012 · International Journal of Systematic and Evolutionary Microbiology
[Show abstract][Hide abstract] ABSTRACT: This report describes the detection of Citrobacter koseri carrying K. pneumoniae carbapenemase (KPC-2) isolated in July 2011 from a Greek patient, who was also colonised by a Klebsiella pneumoniae strain coproducing KPC-2 and Verona integron-encoded metallo-beta-lactamase (VIM)-1.
Full-text · Article · Oct 2011 · Eurosurveillance: bulletin europeen sur les maladies transmissibles = European communicable disease bulletin
[Show abstract][Hide abstract] ABSTRACT: Female genital tuberculosis is an uncommon type of tuberculosis that can lead to infertility. The present review describes the disease, reports available epidemiological data, and focuses on examinations and procedures necessary for the early diagnosis and the management of this curable disease.
No preview · Article · Mar 2011 · Scandinavian Journal of Infectious Diseases
[Show abstract][Hide abstract] ABSTRACT: Multidrug-resistant Acinetobacter baumannii (MDR-AB) infections are difficult to treat owing to the extremely limited armamentarium. The present review reports all available treatment options against MDR-AB, including single molecules, combination schemes, and alternative modes of antimicrobial administration. Additionally, a group of recently reported peptides with anti-MDR-AB activity is described.
Full-text · Article · Feb 2011 · International journal of antimicrobial agents
[Show abstract][Hide abstract] ABSTRACT: Loop-mediated isothermal amplification (LAMP) is a recently developed molecular method that has been successfully implemented in the detection of Mycobacterium tuberculosis in clinical specimens. LAMP has several advantages, such as rapidity, high sensitivity, ease of application and cost-effectiveness. As a result, it is anticipated that its use for the detection of M. tuberculosis is likely to become widespread, especially in low-resource countries. The present review aimed to present this method and all of the available information on its implementation in the detection of M. tuberculosis in clinical specimens.
Full-text · Article · Feb 2011 · European Journal of Clinical Microbiology
[Show abstract][Hide abstract] ABSTRACT: Due to its increased non-susceptibility rates, Klebsiella pneumoniae has emerged as one of the most problematic pathogens.
The level of resistance to 25 antimicrobials of K. pneumoniae isolates from a teaching hospital in Greece and the evolution trends during 2 decades were examined.
A statistically significant increase in non-susceptibility rates was found for almost all antimicrobials examined. During 2008, the isolates presented non-susceptibility rates to aminoglycosides >50% and to quinolones >60%. Nowadays, 1 out of 10 isolates is non-susceptible to colistin. Moreover, the isolates non-susceptible to imipenem were almost doubled between 2007 (29%) and 2008 (50%). Among the imipenem-resistant isolates, 1 out of 4 was also resistant to colistin.
The effectiveness of carbapenems has been compromised and the increase in resistance to colistin is rapid and steep.
[Show abstract][Hide abstract] ABSTRACT: Mycobacterium arupense is a novel mycobacterium species. It was first identified from clinical specimens in 2006 and since then there have been only two reports of its recovery from clinical samples. In the present case M. arupense was isolated from the sputum of a 62-year-old man with a malignant mass in his left kidney, who presented with a one-month history of recurrent fever, dyspnea and haemoptysis. M. arupense was identified with sequencing of hsp65 and 16S rRNA genes. In the present study, its biochemical profile along with its resistance status and hsp65 RFLP analysis is presented.
No preview · Article · Apr 2010 · Indian journal of medical microbiology