Adile Cevikbaş

Marmara University, İstanbul, Istanbul, Turkey

Are you Adile Cevikbaş?

Claim your profile

Publications (26)39.15 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Encapsulation of vancomycin (VANCO) into biodegradable levan microparticles was achieved using a simple preparation technique. Microparticles were prepared by using levan polysaccharide produced by a halophilic bacterium Halomonas smyrnensis AAD6(T). To optimize efficiency of encapsulation process by precipitation method, three parameters were studied: drug and polymer concentrations and preparation rotating speed. The particles were characterized in vitro. The size of levan microparticles was changed between 0.404 μm and 1.276 μm. The surface charge was detected between +4.1 mV and +6.5 mV. The highest drug encapsulation capacity of the system was 74.7% and was depending on the polymer concentration. In dissolution studies, initial burst effect around 10-20% from all the formulations was observed and then the release was slowed down and continued at a constant level. In vitro antibiotic release from the microparticles was controlled with the drug carrier system and release fit to Higuchi kinetic model. All the released samples collected at different time intervals during dissolution studies have exhibited intrinsic bactericidal activity against Bacillus subtilis ATCC 6633. WST-1 cell proliferation and viability studies showed that VANCO-loaded levan microparticles at concentrations between 100 μg/mL and 1000 μg/mL were nontoxic to L929 cells. As conclusion, levan microparticulate system could be a potential carrier of antibiotic drugs such as VANCO.
    No preview · Article · Nov 2015 · Pharmaceutical Development and Technology
  • [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the effect of ciprofloxacin, rifampicine and doxycycline on myeloperoxidase (MPO) activity, glutathione (GSH) and malondialdehyde (MDA) levels in allergic asthma patients and healthy volunteers. Polymorphonuclear leukocytes (PMNs) were isolated with ficoll-hypaque gradient centrifugation method. MPO activity was assayed with modified o-dianisidine, GSH by Ellman's and MDA levels by Beuge's method. PMN functions and MDA levels of patients significantly decreased when compared with healthy volunteers. Ciprofloxacin significantly increased PMN functions, MPO activity and MDA levels of both groups. We have demonstrated that ciprofloxacin has beneficial effects on MPO activity and PMN functions in allergic asthma patients and healthy volunteers.
    No preview · Article · Jun 2015 · Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
  • Source

    Preview · Article · Jan 2014 · Marmara Pharmaceutical Journal
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Today it is known that frequently used antineoplastic agents, analgesics and antibiotics affect the immune system cells and can modify polymorphonuclear leukocyte (PMN) functions such as phagocytosis, lymphocyte proliferation and production of various cytokines. In this study we investigated the effect of antidepresssant drugs on PMN functions, serum folic acid, vitamin B12, zinc, copper levels and hematological parameters in the patients with major depression (MD). Material and Method: PMNs were isolated by Ficoll-Hypaque gradient centrifugation method. Phagocytosis and intracellular killing activity were assayed by modifying Alexander's method. Serum folic acid, vitamin B12, copper and hematology parameters were detected with the biochemical analyzer. Serum zinc levels were measured by flame atomic absorption spectrometer. Results: White blood cell (WBC), neutrophil counts (NE) significantly increased after 2 months of antidepressant drug treatment (p<0.05). The phagocytic activity of PMN of MD patients was found same as healthy volunteers, the intracellular killing activity was found lower than that of healthy volunteers. The PMN's phagocytic activity after one and two months of antidepressant drug treatment insignificantly increased and the intracellular killing activity also insignificantly increased after one month of treatment when compared with those before treatment. The results were not found statistically significant. Serum folic acid, vitamin B12, zinc and copper levels were found in reference value intervals compared to those before treatment. Conclusions: This study showed that WBC, NE counts in patients with MD significantly increased in the reference value intervals after antidepressant drug treatment (p<0.05). We conclude that antidepressant drugs have not deleterious effects on PMN functions, serum folic acid, vitamin B12, zinc, copper levels of patients with MD.
    Full-text · Article · Apr 2013 · Nobel medicus
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, mice were infected with Candida albicans at 07:00 h or 19:00 h. After 24 h, the subgroups of mice received either 0.2/ml saline (as control) or one of two doses (0.5 or 1.0 mg/kg) of amphotericin B (AmB) at 0 h or 12 h for three consecutive days. A second set of uninfected mice received a single dose of either saline or AmB (5 mg/kg) at 0 h or 12 h for 4 days to study only about nephrotoxicity. For uninfected controls and AmB-treated (5 mg/kg) mice, serum blood urea nitrogen (BUN), creatinine and total protein tended to be higher at 0 h vs. 12 h, as was the histopathology score in treated mice (3.60 vs. 1.20). Serum levels changed in treated mice when compared to the control mice. The BUN levels increased whereas serum creatinine levels decreased at 12 h compared to 0 h. C. albicans colony forming units per milliliter (CFU/ml) was high in the kidneys of infected mice. Compared with the control, after treatment for 3 days with 0.5 mg AmB lowered CFU by 48% at 0 h and by 75% at 12 h. However, for the higher dose 1.0 mg AmB, CFU was lowered more or less equally at both test times: 51% at 0 h and 46% at 12 h.
    No preview · Article · Jan 2012 · Biological Rhythm Research
  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the effects of Nigella sativa seed supplementation on symptom levels, polymorphonuclear leukocyte (PMN) functions, lymphocyte subsets and hematological parameters of allergic rhinitis. Twenty-four patients randomly selected from an experimental group of 31 (mean age 34 years) sensitive to house dust mites with allergic rhinitis and a control group of 8 healthy volunteers (mean age 23 years) were treated with allergen-specific immunotherapy in conventional doses for 30 days. After a month of immunotherapy, 12 of the 24 patients and the 8 healthy volunteers were given N. sativa seed supplementation (2 g/day orally) for 30 days. The remaining 12 patients continued only on immunotherapy during the same period. The other 7 patients were given 0.1 ml saline solution subcutaneously once a week as a placebo. The symptom scores, PMN functions, lymphocyte subsets and other hematological parameters were evaluated before and after all treatment periods. There was a statistically significant increase in the phagocytic and intracellular killing activities of PMNs of patients receiving specific immunotherapy, especially after the addition of N. sativa seed. The CD8 counts of patients receiving specific immunotherapy plus N. sativa seed supplementation significantly increased compared to patients receiving only specific immunotherapy. PMN functions of healthy volunteers significantly increased after N. sativa seed supplementation compared to baseline. N. sativa seed supplementation during specific immunotherapy of allergic rhinitis may be considered a potential adjuvant therapy.
    No preview · Article · Mar 2010 · Medical Principles and Practice

  • No preview · Conference Paper · Apr 2009
  • [Show abstract] [Hide abstract]
    ABSTRACT: Zinc supplementation can stimulate polymorphonuclear leukocyte (PMN) functions (phagocytosis and intracellular killing activity) in older hypertensive patients and healthy young volunteers. The aim of this study is to investigate the effect of zinc supplementation on polymorphonuclear leukocyte (PMN) functions (phagocytosis and intracellular killing activity) in vitro in 13 older hypertensive patients and compare these functions with those of 10 healthy young volunteers. PMNs were isolated by ficoll-hypaque gradient centrifugation method from venous blood with EDTA (0.1g/ml).Phagocytosis and intracellular killing activity were assayed by modifying Alexander's method. The subjects were given zinc supplementation 22mg/daily/30 days. Serum zinc levels were measured by flame atomic absorption spectrometer. The serum zinc levels and the PMN's intracellular killing activities of older hypertensive patients before zinc supplementation were significantly low when compared with that of healthy young volunteers (p<0.001, p=0.001 respectively). After zinc supplementation the PMN's phagocytic and intracellular killing activities of older hypertensive patients were significantly lower than those of healthy young volunteers (p<0.001, p<0.05 respectively). However the serum zinc level of the elderly increased after zinc supplementation when compared with that before supplementation . The serum zinc levels and PMN's phagocytic activity of healthy young volunteers significantly increased after zinc supplementation (p<0.05). Consequently, the serum zinc levels of the older hypertensive patients and young volunteers increased after zinc supplementation. Adequate zinc supplementation may improve PMN functions both in older hypertensive patients and healthy young volunteers.
    No preview · Article · Jan 2009 · Turkish Journal of Pharmaceutical Sciences
  • Source

    Full-text · Article · Jan 2009
  • E. Tuna · P. Rayaman · A. Çevikbaş · E. Rayaman · U. Soyoǧul Gürer
    [Show abstract] [Hide abstract]
    ABSTRACT: In our study the effects of the antidepressant drugs buspirone (0.0025/μg/ml), sertraline (0.19/μg/ml), citalopram (0.286/μg/ml, 0.502/μg/ml, 0.703/μg/ml) and fluoxetine (0.015/μg/ml, 0.035/μg/ml, 0.055/μg/ml) on polymorphonuclear leukocyte (PMN) function of 20 healthy young volunteers, whose mean age was 25 were investigated in vitro. PMNs (l×l07cell/ml) were isolated by ficoll-hypaque gradient centrifugation method from venous blood with EDTA. Phagocytosis and intracellular killing activity were assayed by modifying Alexander's method. Fluoxetine at 0.035/μg/ml concentration has significantly increased the PMN's phagocytic activity, of the healthy young volunteers but did not effect their PMN's intracellular killing activity. The results might bring a new immunotherapeutic approach to the therapy of the major depressant patients whose immune system is suppressed.
    No preview · Article · Jan 2009 · Turkish Journal of Pharmaceutical Sciences
  • Ç. Ünsal · G. Sarıyar · B. Gürbüz Akarsu · A. Çevikbaş
    [Show abstract] [Hide abstract]
    ABSTRACT: Three alkaloids, cheilantifoline, mecambrine, and laudanosine, and two flavonoids, luteoline and tricine, have been isolated from two samples of Papaver macrostomum. Boiss. & Huet ex Boiss. (Papaveraceae) of Turkish origin. Antimicrobial tests have been performed on the extracts obtained from these species. It has been found that diethyl ether and acetone extracts of two samples obtained from the aerial parts of the plant have antimicrobial activity against almost all bacteria tested. The existence of flavonoids and the antimicrobial activity of this species are reported for the first time.
    No preview · Article · Oct 2008 · Pharmaceutical Biology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In order to investigate the effect of kefir consumption on mucositis induced by 5-FU based chemotherapy (CT), we monitored the systemic immune response by measurement of the serum proinflammatory cytokine levels and we evaluated the anti-microbial effect of kefir with an agar diffusion method. Forty patients with colorectal cancer were included in this randomized prospective study. On the first 5 days of each CT cycle, the study group received oral lavage with kefir and then swallowed 250 ml of kefir while control group received oral lavage with 0.09% NaCl twice a day. Before and after every cycle of CT, the oral mucosa was assessed. Serum proinflammatory cytokine levels were evaluated before the initiation and after the third and the sixth cycle. Kefir was administered in 99 out of 205 courses. Mucositis developed in 27.3% of the courses given with kefir administration and in 21.7% of the courses given with 0.9% NaCl oral rinses. The difference between the two groups was not statistically significant (p > 0.05). When we compared the serum proinflammatory cytokine levels of the two groups at the baseline and following the third and the sixth cycles, we again found no statistically significant difference (p > 0.05). Kefir consumption at the mentioned doses made no statistically significant effect on serum proinflammatory cytokine levels and on the incidence of mucositis development in cancer patients. Under in vitro conditions, kefir inhibits only Staphylococcus epidermidis.
    Full-text · Article · Sep 2008 · Investigational New Drugs
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate changes in phagocytic activity of neutrophils of type 2 diabetic patients with foot infections over short treatment courses. The potential utility of the phagocytic index in determining the efficacy of treatment modalities and it's relationship with metabolic control parameters were evaluated. The phagocytic activity of neutrophils was determined in blood samples of 38 type 2 diabetic patients with foot infections (14 women and 24 men). Mean age and mean duration of diabetes were 66.3+/-9.4 and 19.1+/-11.2 (yrs), respectively. All patients received standard treatment (intensive insulin therapy, antibiotherapy, hyperbaric oxygen therapy and surgical debridement). Phagocytic activity of neutrophils was determined by a standard method. Phagocytic activity of neutrophils, acute phase proteins (C-reactive protein) and glycosylated haemoglobin was determined before therapy and two weeks later. The phagocytic index before and after therapy were 47.7+/-11.4 and 62.5+/-15.6, respectively (p<0.05). There was a significant correlation between phagocytic index and both CRP and HbA1c (r=0.52, p<0.05 and r=-0.41, p<0.05, respectively). Derangement of carbohydrate metabolism may underlie the impairment of bactericidal activity of neutrophils of poorly controlled diabetic patients. These data reveal that phagocytic activity improves during short-course standard therapy and might enable monitoring of efficacy of treatment modalities in diabetic patients with foot infections.
    No preview · Article · Oct 2007 · The Journal of infection
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study was carried out prospectively to determine the effect on prognosis of phagocytic activity index (PAI) and intracellular killing activity (IKA) of polymorphonuclear leukocytes (PMNL), and the levels of interleukin-1 beta (IL-1beta) on prognosis in patients with diabetic foot infection (DFI). The evaluation of PAI and IKA in PMNL and the levels of IL-1beta were performed at the beginning and in the second and fourth weeks of therapy in all diabetic patients, who were categorized into a healing group (HG) and a non-healing group (NHG) on the basis of therapy results. Sixty-six cases (38 diabetic patients and 28 non-diabetic controls) were included in the study. Full recovery was observed in 23 HG patients, whereas 15 (NHG) patients were unresponsive to treatment and nine patients were subjected to amputation at the end. At the baseline, PAI, IKA and IL-1beta levels in HG were not significantly different compared to those of NHG, but at weeks 2 and 4, PAI and IKA levels were significantly higher and IL-1beta levels were significantly lower than those in NHG. On the other hand, at the baseline, PAI and IKA values in HG were significantly lower and IL-1beta levels were significantly higher in comparison with the controls. However, no significant difference was observed at week 2 or 4. Our results suggest that the PMNL functions and IL-1beta regulation deteriorated in patients with DFI, and that such deteriorations might indicate inefficient therapeutic responses in patients with diabetes mellitus.
    No preview · Article · Apr 2007 · The Journal of infection
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effects of ciprofloxacin, cefodizime, rifampicine, doxycycline and cefodizime + rifampicine combination on polymorphonuclear leukocyte (PMN) functions (phagocytosis and intracellular killing activity) were investigated in vitro in elderly patients and compared with those of healthy young volunteers before and after zinc supplementation. PMNs of 13 elderly hypertensive patients and 10 healthy young volunteers were isolated by Ficoll-Hypaque gradient centrifugation method from venous blood with EDTA. The subjects were given 22 mg/daily/oral zinc supplementation for 1 month. Serum zinc levels before and after supplementation were measured by flame atomic absorbtion spectrophotometer and the effects of each drug on PMN functions at therapeutic concentrations were investigated. Ciprofloxacin significantly increased the PMN's phagocytic activity of elderly patients (p = 0.002) before zinc supplementation and significantly increased both PMN functions of elderly patients (p = 0.002) after zinc supplementation. The same antibiotic significantly increased both PMN functions of healthy young volunteers (p = 0.005 and p<0.05, respectively) before and after zinc supplementation when compared with the control (drug-free). Cefodizime significantly increased the PMN's phagocytic activity of elderly patients (p = 0.003, p = 0.002) before and after zinc supplementation when compared with the control (drug-free). It also significantly increased both PMN functions of healthy young volunteers (p = 0.005 and p<0.05, respectively) before and after zinc supplementation when compared with the control (drug-free). Doxycycline significantly increased PMN's intracellular killing activity of healthy young volunteers before zinc supplementation (p<0.05) when compared with the control (drug-free) values. Rifampicine significantly decreased PMN's phagocytic activity of elderly patients (p<0.05) after zinc supplementation. Cefodizime+rifampicine combination significantly increased PMN's phagocytic activity at therapeutic concentrations of healthy young volunteers (p = 0.005) before zinc supplementation and PMN's phagocytic activity of elderly patients (p<0.05) after zinc supplementation when compared with the control (drug-free). Consequently, in the present study from the antibiotics ciprofloxacin, cefodizime and cefodizime + rifampicine combination, which are accepted as biological response modifiers have demonstrated stimulatory effects by significantly increasing polymorphonuclear leucocyte functions (phagocytosis and/or intracellular killing activity) of elderly patients and healthy young volunteers in vitro before and after zinc supplementation. Additionally zinc supplementation has more immunostimulatory effects on PMN functions of healthy young volunteers than elderly patients.
    No preview · Article · May 2006 · International Immunopharmacology
  • [Show abstract] [Hide abstract]
    ABSTRACT: To compare the polymorphonuclear leukocyte (PMN) functions (phagocytosis and intracellular killing activity) of elderly patients with healthy young volunteers. Fifty-nine elderly patients who had various diseases (cancer, hypertension and diabetes mellitus, DM) and 10 healthy young volunteers were included in this study. Ficoll-Hypaque gradient centrifugation was used to isolate PMNs from venous blood containing EDTA (0.1 g/ml). Phagocytosis and intracellular killing activity of neutrophils were assayed using a modification of Alexander's method, in which serum opsonins, number of neutrophils and number of microorganisms are standardized in order to detect both increases and decreases in phagocytosis and intracellular killing as well as combined abnormalities of these two functions. The least significant difference test was used to compare the results in the two groups. Phagocytic activity of PMNs from patients with cancer was significantly higher than that of healthy young volunteers (p < 0.05) and elderly patients with hypertension and DM (p < 0.05). There was no statistical difference between the phagocytic activity of PMNs from elderly patients with hypertension and DM and healthy young volunteers (p > 0.05). The intracellular killing activity of PMNs from elderly patients with hypertension, DM and cancer was significantly lower than that of healthy young volunteers (p = 0.001, p < 0.0001, p = 0.003, respectively). The intracellular killing activity of PMNs from elderly patients was significantly decreased when compared with that of healthy young volunteers. Ageing, chronic diseases and drugs used in the treatment of these elderly patients may be the cause for decreased intracellular killing activity.
    No preview · Article · Nov 2005 · Medical Principles and Practice
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the study was to investigate antituberculous drugs effects on polymorphonuclear leukocyte (PMN) functions (phagocytic activity and intracellular killing activity) in vitro. PMNs obtained from healthy volunteers were incubated with antituberculous drugs (isoniazid [INH], rifampin [RIF], pyrazinamide [PZA], ethambutol [EMB], streptomycin [S], amikacin [A], ofloxacin [OFLX], prothionamide [PTH] and cycloserine [CyC]) and different combinations at therapeutic serum concentrations. Phagocytic activity of PMNs was significantly increased when compared with controls by PTH (p<0.001), A (p<0.001), OFLX (p<0.001), INH+RIF+S combination (p<0.01), A+OFLX combination (p<0.05), A+OFLX+CyC combination (p<0.01) and A+OFLX+CyC+PTH+EMB combination (p<0.01). Intracellular killing activity of PMNs was significantly increased by OFLX when compared with the control (p<0.05). No significant difference was observed in functions of PMN for other drugs when compared with control (p>0.05). Functions of PMN were significantly increased by OFLX when compared with A+OFLX combination (p<0.05). Phagocytic activity of PMNs was significantly increased by A+OFLX+CyC combination and A+OFLX+CyC+PTH+EMB combination when compared with A+OFLX+CyC+PTH combination and A+OFLX+CyC+PTH+PZA combination (p<0.05). No significant difference was found in functions of PMN between the other groups (p>0.05). In conclusion, some antituberculous drugs alone or in combination enhanced PMN functions, although in combination no additive or synergistic effects were detected. Moreover, none of the antituberculous drugs alone or in combination significantly decreased PMN functions. The drugs having adverse effects on immune functions would better be replaced with equally effective drugs or drug combinations having positive effects on PMN functions.
    No preview · Article · Jul 2005 · International Immunopharmacology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Immunotherapy plays an important role in the therapy of allergic rhinitis and bronchial asthma. However, there is not much information about the effects of allergen-specific immunotherapy (SIT) on the polymorphonuclear leukocyte functions. The aim of this study was to investigate the effects of specific immunotherapy on phagocytic and intracellular killing activities of polymorphonuclear leukocytes (PMN) derived from patients with seasonal allergic rhinitis. Twenty-four patients with seasonal allergic rhinitis documented to be sensitive to grass pollen were included in this study. Patients were divided into 3 groups. Group 1 (n=7) received conventional immunotherapy whereas patients in Group 2 (n=7) were treated with short-term immunotherapy and the third group (n=10) were given placebo during the study process. Both phagocytic and intracellular killing activities were significantly increased (p=0.002, p<0.0001, respectively) by conventional immunotherapy when compared to the first determination. In the short-term immunotherapy group, phagocytic activity was increased very significantly (p=0.0001), whereas intracellular killing activity was not affected (p=0.252). There were no changes in these parameters in the placebo group. These results suggest that allergen-specific immunotherapy has an enhancing effect on PMNs functions in the patients with seasonal allergic rhinitis. It should be clarified by further studies whether this enhancement might be considered as another beneficial effect of the immunotherapy.
    No preview · Article · May 2005 · International Immunopharmacology
  • Necdet Duman · Adile Cevikbaş · Candan Johansson
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study was to determine the activities of rifampicin, ciprofloxacin and sparfloxacin against M. tuberculosis H37Rv in human macrophages. The minimal inhibitory concentrations (MIC) were determined by agar macrodilution and broth microdilution methods and were 0.5, 1 and 0.125 mg/l, respectively. Concentrations of rifampicin at 0.5 and 2.5 microg (P<0.001), ciprofloxacin at 4 and 8 microg (P<0.001) and sparfloxacin at 0.125 microg (P<0.05), 0.625 microg (P<0.001) and 1.25 microg (P<0.001) were found to be effective against intracellular bacteria. Ciprofloxacin and especially sparfloxacin were effective in macrophages and may be useful in the treatment of tuberculosis particularly infections caused by multiply drug resistant strains.
    No preview · Article · Jan 2004 · International Journal of Antimicrobial Agents
  • [Show abstract] [Hide abstract]
    ABSTRACT: Leukotriene receptor antagonists are being used widely in the treatment of bronchial asthma. They have been shown to possess anti-inflammatory properties, but there is no sufficient data about their effects on polymorphonuclear leukocyte functions. The aim of this study was to investigate the effects of montelukast, a specific cysteinyl leukotriene-1 receptor antagonist, on human polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) in asthmatic patients. Fifteen mild to moderate asthmatic patients were included in the study. They were treated with montelukast (10 mg/day per os) in addition to their previous medications for 2 weeks. Whole blood samples of patients were taken before and after this treatment period. Phagocytic activities and intracellular killing activities of polymorphonuclear leukocytes isolated from whole blood samples were tested by using appropriate technics. Phagocytic and intracellular killing activities of PMNs were significantly increased (p<0.001, p<0.05) by montelukast compared to those before treatment. These results show that montelukast has an enhancing effect on PMN functions in asthmatic patients.
    No preview · Article · Sep 2003 · International Immunopharmacology