Sema Bircan

T.C. Süleyman Demirel Üniversitesi, Hamitabat, Isparta, Turkey

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Publications (39)42.25 Total impact

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    ABSTRACT: EGFR and KRAS mutation profile in non-small cell lung cancers (NSCLCs) shows wide variations due to geographic and ethnic background. We aimed to determine the frequency and types of EGFR and KRAS mutations in a sample group of Turkish NSCLC cases. The study included 14 adenocarcinomas (ACs), 11 squamous cell carcinoma (SCC) patients selected from archival material including small biopsy or surgical specimens. Their formalin fixed paraffin-embedded tumor tissues were used for genomic DNA extraction for EGFR exon 19 and 21, and KRAS exon 2 mutations. Eleven NSCLCs (44 %) had EGFR mutations. Exon 19 and 21 mutations were found in 8 (32 %) and 5 (20 %) cases. Two cases showed double EGFR mutations. In ACs, 5 (35.7 %) patients had EGFR gene mutation, 3 in exon 19 and 3 in exon 21. In SCCs, 6 (54.5 %) cases had EGFR mutation, 5 in exon 19 and 2 in exon 21. All exon 19 mutations were deletion-type mutations. For exon 21, 3 cases had L858R point mutation (CTG>CGG) and two cases showed deletion-type mutations. Six (24 %) NSCLCs showed KRAS mutations (three ACC, three SCC), 5 codon 12 mutations (G>T, T>C, G>A) and one codon 13 mutation (G>T). Three NSCLC cases showed both EGFR and KRAS mutations together. The profile of KRAS mutation in our AC cases was quite similar to those seen in the Western countries; however, frequency and clustering of EGFR mutations were similar to those seen in the Eastern countries.
    No preview · Article · Aug 2014 · Medical Oncology
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    ABSTRACT: The aim of the present study was to evaluate the expressions of beclin 1 and bcl-2 in prostate cancer (PC) and high grade prostatic intraepithelial neoplasia (HGPIN), and to investigate their relationship with clinicopathological parameters. The study included 30 benign prostatic hyperplasia (BPH), 40 HGPIN and 106 primary PC cases. The expressions of beclin 1 and bcl-2 were assessed semiquantitatively based on both the percentage and intensity of positive staining cells. Beclin 1 was positive in 27 (90%) BPH, 37 (92.5%) HGPIN, and 90 (84.9%) PC cases (p>0.05). Bcl-2 immunostaining was detected in 99 (93.4%) PC, 37 (92.5%) HGPIN, and 9 (30%) BPH cases (p<0.0001). Regarding expression scores, beclin 1 was significantly lower in PC cases than in the HGPIN and BPH groups (p<0.0001), and it was also negatively correlated with Gleason score (p=0.004, r=-0.274). Bcl-2 expression score was significantly higher in PC than in the other groups (p<0.0001), and also positively correlated with Gleason score (p<0.0001, r=0.425). Furthermore, a negative correlation was found between bcl-2 and beclin 1 expression scores in PC cases (p=0.006, r=-0.265). Our results suggest an association between bcl-2 and beclin 1 expressions in malignant transformation of prostate tissue and also in regulating PC cell differentiation, progression and the agressiveness of PC.
    No preview · Article · Jul 2014 · Pathology - Research and Practice
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    ABSTRACT: Purpose: Vasectomy is one of the most common urological operations performed, and provides permanent contraception. Many vasectomized men ultimately seek vasectomy reversal because of unforeseen changes in lifestyle. Vasovasostomy has varying rates of success. In this study, we utilize vas deferens (VD), artery, and vein grafts to reconstruct 30% and 50%defects of the total vas deferens length. Materials and methods: Forty two male Wistar rats were divided into three groups as VD graft, carotid artery and external jugular vein transplantations. Each group was equally divided into 2 different subgroups according to the length of transplant material as 1.0 cm (n = 7) and 1.5 cm (n = 7). To evaluate whether these materials may be used for long segment vas deferens reconstruction, the patency rate, partial or total graft occlusion, and histologic examination of all specimens were examined. Results: No patency was found in any of the grafts and many of them suffered destructive changes in anatomic structure. Sperm granulomas were determined around the testicular side anastomosis due to accumulated semen fluid which was in our belief, a result of aperistaltic zone caused by the grafts. Conclusion: When the poor results obtained in our study are put into perspective, vasoepididymostomy is the only treatment method to date for reconstruction of large segment vas deferens defects.
    No preview · Article · May 2014 · Urology journal
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    ABSTRACT: Beclin 1 plays a critical role in the regulation of autophagy, apoptosis, differentiation, as well as in the development and progression of cancer. The aim of this study was to examine the expression of beclin 1 and bcl-2 in bladder urothelial tumors, and to investigate the relationship between these two markers and clinicopathological parameters. Our study included 84 bladder urothelial tumors and 10 non-tumoral bladder tissues. Immunohistochemistry was performed on tissue microarray (TMA) sections and was evaluated semiquantitatively on the basis of the percentage of positively stained cells (proportion) and staining intensity. A significant association was found between the expression score of beclin 1 and pT stages of the urothelial tumors (p=0.012). Also, the level of beclin 1 expression inversely correlated with histological grade and pT stages (p=0.009, r=-0.284; p=0.001, r=-0.361, respectively). The bcl-2 expression level positively correlated with histological grade and pT stages of the urothelial tumors (p=0.026, r=0.243; p<0.0001, r=0.491, respectively). In addition, the level of beclin 1 expression tended to be inversely correlated with the bcl-2 expression level in urothelial tumors (p=0.055, r=-0.210). According to our data, down-regulation of beclin 1 expression and also bcl-2 overexpression seem to play an important role in the progression and aggressiveness of bladder urothelial tumors.
    No preview · Article · Apr 2013 · Pathology - Research and Practice
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    ABSTRACT: Objective: The aim of our study was to determine the incidence and clinicopathological features of incidental prostate carcinoma in patients who underwent radical cystoprostatectomy for bladder carcinoma. Material and Methods: Radical cystoprostatectomy materials of 59 patients with urothelial carcinoma were included in this study. There was no preoperative evidence of prostate carcinoma in any case. The clinicopathological features of the bladder tumors and accompanying prostate carcinomas were obtained retrospectively from the pathology reports, and their relations were investigated. Results: Nine out of 59 (15.3%) cases had incidental prostate adenocarcinoma. The median age was significantly higher in cases with accompanying prostate adenocarcinoma (71.0) compared to those without (65.0) (p=0.012). Gleason scores of these cases were 6 in 8 (88.9%) cases and 7 (3+4) in 1 (11.1%) case and pathologic tumor (pT) stages were distributed as pT2a in 3 (33.3%) cases, pT2b in 1 (11.1%) case, pT2c in 3 (33.3%) cases, pT3a in 1 (11.1%) case, and pT3b in 1 (11.1%) case. Two (22.2%) out of 9 cases of co-existing prostate adenocarcinoma had low-grade, and 7 (77.8%) had high-grade urothelial carcinoma; their pT stages were distributed as pT1, pT2, pT3 and pT4 in 1(11.1%), 5 (55.6%), 1 (11.1%) and 2 (22.2%) cases, respectively. The pT stage of bladder carcinomas with accompanying prostate carcinomas tended to have a lower stage than the cases without prostate cancer, although the difference was not significant (p=0.057). Moreover, no significant relation was found regarding histologic grade (p=1.000). Conclusion: Accompanying prostate adenocarcinoma should be considered in patients who underwent cystoprostatectomy due to bladder tumor. We suggest further investigation of a relationship if any between prostate and bladder carcinoma in larger patient groups.
    No preview · Article · Jan 2013 · Turkiye Klinikleri Journal of Medical Sciences
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    ABSTRACT: Lymphomas originating from lung constitute less than 1% of all non-Hodgkin lymphomas. Most of them are MALT (mucosa-associated lymphoid tissue) lymphomas originating from bronchial mucosa-related lymphoid tissue. Extranodal MALT lymphomas are slowly progressing lymphomas involving mucosa-related lymphoid tissue (MALT), they remain as a localized mass for a long time and bone marrow and peripheral lymph node involvements are seen very rarely. Patient survival is long and the response rate to treatment is high. In this paper, we present a 56-year- old male patient in whom an incidental mass lesion was detected in the right lung on chest radiography obtained when he was being followed up for brain edema. Widespread lymphoid cell infiltration was seen in the initial bronchoscopic tissue biopsy, and he was diagnosed as MALT- type extranodal marginal zone lymphoma after examination of the wedge resection material. The case was discussed in the lightof radiological, clinical and pathological findings.
    Preview · Article · Jan 2012 · Turkiye Klinikleri Journal of Medical Sciences
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    ABSTRACT: A considerable number of recent reports have documented mycosis fungoides resembling many other dermatoses. Due to highly variable presentations and the sometimes non-specific nature of histological findings, an accurate diagnosis of mycosis fungoides can be difficult. Erythema annulare centrifugum-like mycosis fungoides with a variety of annular, polycyclic erythematous skin lesions is a recently recognized atypical manifestation of mycosis fungoides, and only a few cases have been reported to date.
    No preview · Article · Nov 2011 · Australasian Journal of Dermatology
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    ABSTRACT: A 23-year-old man was admitted to our outpatient clinic complaining of infertility and bilateral testicular masses. At physical examination nodular lesions were detected in bilateral testes. The medical history revealed congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency since the age of 2 years. Serum tumor markers were within the normal range. The patient underwent high inguinal testicular exploration; and since differential diagnosis of testicular tumor could not be made with frozen section, high inguinal orchiectomy was performed. The diagnosis of testicular adrenal rest tumor had been confirmed by clinical, morphological, immunohistochemical, laboratory, and endocrinological evaluations, and the patient was referred to endocrinology clinic for future treatment. Clinical control was recommended once a year for testicular masses in left testes.
    No preview · Article · Sep 2011 · Turk Uroloji Dergisi
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    ABSTRACT: Primary non-Hodgkin lymphoma of testis is an rare disease. It is usually seen in elderly patients. Diffuse large B cell lymphoma is the most common histological type. Clinical stage is the most important prognostic factor. Primary testicular lymphoma (PTL) is infrequent whereas involvement of testis is common in nodal lymphomas. Fifty seven percent of cases with PTL present with stage I, 22% with stage II and 21% with stage III-IV. Randomized prospective controlled prospective studies are not available for treatment of PTL due to rarity of the disease. The most common presentation is painless scrotal mass. We hereby, report a case with PTL and reviewed the literature since it is a rare entity, and there are no standard treatment modalities.
    Full-text · Article · Jan 2011 · Turkiye Klinikleri Journal of Medical Sciences
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    ABSTRACT: Pulmonary carcinosarcoma, belonging to sarcomatoid carcinomas, is a quite rare tumor that contains both malignant epithelial and mesenchymal elements. This tumor has different phenotypic characteristics and clinical course compared to non-small cell lung tumors. A case diagnosed as carcinosarcoma is presented and its clinical and pathological features and the differential diagnosis are discussed. The case was a 74-year-old male admitted with shortness of breath and cough. The chest x-ray showed a left lung mass and a bronchoscopic examination was performed. Histopathological examination of the bronchoscopic biopsy showed necrosis and a malignant tumor consisting of diffuse infiltrative anaplastic cells. Surgery was performed and the case was diagnosed as carcinosarcoma in the resection material. Pulmonary carcinosarcoma is a rare lung tumor. Determination of tumoral cells and performing advanced investigations in resection material seem to be relatively easier than in small biopsies. However, this type of tumor can be encountered in small biopsy materials as in the presented case and should be kept in mind in relation to the differential diagnosis as small tissues can have only one, particularly mesenchymal, tumoral component.
    Full-text · Article · Jan 2011 · Turk Patoloji Dergisi
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    ABSTRACT: Maspin, one of the serine protease inhibitors, has been shown to inhibit tumor progression and metastasis. We aimed to investigate maspin, p53 and VEGF expression in patients with squamous cell carcinoma (SCC), adenocarcinoma (AC) and small cell lung carcinoma (SCLC). The study included 28 SCC, 18AC, 17 SCLC biopsy samples. We used the streptavidin biotin immunoperoxidase method to test for maspin, p53 and VEGF antibodies. Medical records of these patients were reviewed from archival files. Cytoplasmic maspin expression was detected in 89.3%, 77.8%, 52.9% of SCC, AC and SCLC, respectively. The rate was significantly higher in non-small cell lung cancer (NSCLC) and SCC than SCLC (p = 0.013, p = 0.021, respectively). The mean percentages of maspin expression were significantly higher in NSCLC, SCC and AC than in SCLC (p = 0.0001, p = 0.0001, p = 0.038, respectively). In ACs, maspin and p53 expressions were correlated, although this was not statistically significant (p = 0.053, r = 0.464), and maspin positive cases had a significantly higher T status compared to negative cases (p = 0.036). In SCC, the stage of disease was positively correlated with p53 (p = 0.007, r = 0.536) and negatively correlated with VEGF expression (p = 0.013, r = -0.498). Multivariate analysis demonstrated that stage of disease was a significant independent prognostic parameter in NSCLC (95% confidence interval: 1.067-3.969; p = 0.031). Although maspin expression is higher in SCC and AC, and is related with higher T status in AC, our data did not indicate its prognostic significance. Larger scale studies are needed to reveal the exact role of maspin in lung cancer pathogenesis.
    No preview · Article · Mar 2010 · Pathology & Oncology Research
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    ABSTRACT: Neurofibromatosis (NF) is a genetic disorder of the nervous system that primarily affects the development and growth of neural cell tissues. This disorder is characterized by the development of various tumors, including neurofibromas, neuroniomas, malignant and benign peripheral nerve sheath tumors, and meningiomas. Accompanying skin changes and bone deformities are also common in NF. However, genitourinary involvement in NF is a rare condition, and penile enlargement has been reported only in a few males with plexiform NF. We report a 6-year-old boy with chronic renal failure associated with plexiform neurofibromas of the bladder and prostatic urethra which led to urinary obstruction and macrogenitalia due to genitourinary NF.
    Full-text · Article · Oct 2009 · Pediatric Nephrology
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    ABSTRACT: Idiopathic hypereosinophilic syndrome, a rarely seen systemic disease, may cause cardiac valvular lesions by eosinophilic infiltration. This report describes management of a 25-year-old woman with idiopathic hypereosinophilic syndrome, severe mitral stenosis, and pulmonary arterial hypertension. The patient was presented with haemoptysia and dyspnea on exertion. Echocardiography showed severe mitral stenosis and pulmonary arterial hypertension. After hematological stabilization, she underwent mitral valve replacement using a No. 27 bovine pericardial valve. In the intensive care unit she had a pulmonary hypertensive crisis, which ameliorated gradually with sedation and nitroglycerin. She was extubated and discharged on the second and seventh days, respectively. Surgical experience for the patients with mitral dysfunction caused by idiopathic hypereosinophilic syndrome is limited. When mitral valve replacement is needed, the ideal type of prosthesis remains unclear and the presence of pulmonary arterial hypertension further complicates the management. We think that bioprosthetic valves would be the appropriate choice in eosinophilic mitral dysfunction requiring valve replacement.
    No preview · Article · Jan 2009 · Journal of Cardiac Surgery
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    ABSTRACT: Galectin-3 is a ss-galactoside-binding lectin. It participates in a variety of normal and pathologic processes, including cancer progression. In this study, we evaluated the pattern of expression of galectin-3 in cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC), and its correlation with the grade of differentiation in SCC and tumor size. Galectin-3 expression was evaluated by immunohistochemistry in 31 SCCs, 30 BCCs, and 29 non-tumoral skin samples. Galectin-3 expression was higher in normal epidermis than in non-melanoma skin cancers, except for cytoplasmic immunoreactivity in SCC. Cytoplasmic galectin-3 immunoreactivity was significantly higher than nuclear immunoreactivity in non-melanoma skin cancers. Cytoplasmic galectin-3 immunoreactivity was significantly higher in SCC than in both circumscribed and infiltrative BCCs, but no difference was detected between these two types of BCC. Cytoplasmic galectin-3 immunoreactivity predominated within SCCs (p=0.000), and a positive correlation was detected between tumor size and cytoplasmic immunoreactivity (r=0.385, p=0.043). There was no correlation between galectin-3 staining and tumor differentiation and lymph node metastasis. Decreased nuclear galectin-3 expression and cytoplasmic immunoreactivity in tumors are important factors in the progression from the normal to the cancerous state in non-melanoma skin cancers. We speculate that cytoplasmic galectin-3 expression may be one of the factors that contribute to tumor aggressiveness in SCC.
    No preview · Article · Nov 2008 · Pathology - Research and Practice
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    ABSTRACT: Süleyman Demirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2008 Mart; 15 (1) Primer akciğer tümörlerinde prebronkoskopik ve postbronkoskopik balgam sitolojileri Kemal K. Bozkurt*, Korkut Bozkurt*, Şirin Başpınar**, I. Metin Çiriş*, Sema Bircan*, Nermin Karahan*, F. Nilgün Kapucuoğlu* * Süleyman Demirel Üniversitesi Tıp Fakültesi Patoloji AD. **Eğirdir Kemik Eklem Hastalıkları Tedavi ve Rehabilitasyon Hastanesi. Özet Amaç: Primer akciğer tümörü ön tanısıyla bronkoskopi yapılarak biyopsi alınan olguların prebronkoskopik ve postbronkoskopik balgam sitolojisi sonuçlarını histopatoloji sonuçlarıyla karşılaştırarak fakültemizde balgam sitolojilerinin etkinliğinin araştırılması. Materyal ve Metod: SDÜ Tıp Fakültesinde 2002-2005 yılları arasında bronkoskopi yapılarak biyopsi alınan 64 hastaya ait prebronkoskopik ve/veya postbronkoskopik balgam sitolojisi sonuçları histopatoloji sonuçlarıyla karşılaştırıldı. Sitoloji sonuçları benign, malignite kuşkulu, malign ve yetersiz olarak sınıflandırıldı. Bulgular: Incelenen 48 adet prebronkoskopik balgam örneğinin 37.si (%77.1) benign, 2.si (%4.2) yetersiz, 5.i (%10.4) malignite kuşkulu, 4.ü (%8.3) malign olarak rapor edilmiştir. Prebronkoskopik balgam sitolojisinin duyarlılığı %28.6, özgüllüğü %84.4, tanı değeri %67.4.dür. Incelenen 42 adet postbronkoskopik balgam örneğinden 32.si (%76.2) benign, 3.ü (%7.2) yetersiz, 6.sı malignite kuşkulu (%14.2), 1.i (%2.4) malign olarak rapor edilmiştir. Postbronkoskopik balgam sitolojisinin duyarlılığı %29.2, özgüllüğü %100, tanı değeri %56.4.dür. Sonuç: Balgam sitolojisi primer akciğer tümörlerinde ucuz ve noninvaziv bir tanı yöntemidir. Anahtar kelimeler: Akciğer kanseri, bronkoskopik biyopsi, prebronkoskopik balgam, postbronkoskopik balgam, duyarlılık Abstract Prebronchoscopic and postbronchoscopic sputum cytologies in primary lung tumors Purpose: To determine the utility of the sputum cytology in the evaluation of the primary lung tumors in our faculty by comparing the cytology results and histopathology results of patients who underwent bronchoscopy. Material and Method: We compared the prebronchoscopic and postbronchoscopic sputum cytology results and histopathology results of 64 patients who underwent bronchoscopy between 2002 and 2005 in Süleyman Demirel University School of Medicine. Cytology results were categorized as benign, suspicious for malignancy, malignant and insufficient. Results: The diagnosis of prebronchoscopic sputum cytology were benign, insufficient, suspicious for malignancy and malignant in 37(77.1%), 2(4.2%), 5(10.4%), 4(8.3%) cases respectively. Sensitivity, specificity, accuracy of the prebronchoscopic sputum cytology were 28.6%, 84.4%, 67.4% respectively. The diagnosis of postbronchoscopic sputum cytology were benign, insufficient, suspicious for malignancy and malignant in 32(76.2%), 3(7.2%), 6(14.2%), 1(2.4%) cases respectively. Sensitivity, specificity, accuracy of the prebronchoscopic sputum cytology were 29.2%, 100%, 56.4% respectively. Conclusion: Sputum cytology is a cheap and noninvazive diagnostic method for primary lung tumors. Keywords: Lung cancer, bronchoscopic biopsy, prebronchoscopic sputum, postbronchoscopic sputum, Sensitivity
    Full-text · Article · Jan 2008
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    ABSTRACT: PTEN is a tumor suppressor gene that is frequently mutated in type I endometrioid endometrial carcinomas (EECs), and is involved in the control of cell proliferation, differentiation, and apoptosis. In this study, we aimed to assess the relationship between PTEN expression and estrogen, progesterone receptors (PRs), other apoptosis-related proteins, such as bcl-2 and bax, and apoptotic index (AI) in EEC, its precursor lesion hyperplasia, and cyclical endometrium. We also evaluated the relationship between PTEN expression and clinicopathologic parameters. PTEN, estrogen receptor (ER), PR, and bcl-2 and bax expressions were evaluated immunohistochemically, and AI was evaluated in hematoxylin and eosin (HE)-stained slides in 23 cyclical and 37 hyperplastic endometria and in 35 EECs. PTEN expression was higher in cyclical endometrium than in the carcinomas (p<0.05). The PTEN expression level was significantly higher in non-atypical hyperplasias than in EEC, but there were no differences between atypical complex hyperplasia (ACH) and EEC and between hyperplasias. In the carcinomas, there was a negative correlation between grade and PTEN expression (r=-0.338, p=0.047). In conclusion, we presume that PTEN is involved in the early phases of endometrial tumorigenesis, and it can be speculated that decreased PTEN expression with loss of differentiation in carcinoma can contribute to the emergence of tumors with a more aggressive phenotype.
    No preview · Article · Mar 2007 · Pathology - Research and Practice
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    ABSTRACT: Anthracotic pigmentation in the bronchial mucosa has been regarded as a bronchoscopic finding of pneumoconiosis or evidence of heavy atmospheric soot. Anthracotic pigmentation with bronchial narrowing or obliteration, surrounded by calcified or noncalcified lymph nodes is typical finding of anthracofibrosis. There is a potential relationship between bronchial anthracofibrosis and tuberculosis. Tuberculous lymphadenopathy of superior mediastinum presentation with hoarseness is very rare. The paper reports a case of tuberculous mediastinal lymphadenitis with anthracosis causing vocal cord paralysis. A 66-year-old woman was admitted to our clinic with the symptoms of dry cough, hoarseness, malaise, anorexia, night sweats and with the multiple mediastinal lymphadenopathy. Fiberoptic bronchoscopy revealed left vocal cord paralysis, bronchial mucosal inflammation and multiple anthracotic plaques. Bronchial lavage and mucosal biopsy were negative for malignancy and tuberculosis. The thoracotomy was performed and a mediastinal lymph node showing caseating granulomatous inflammation with anthracosis and parenchymal anthracosis were detected. The diagnosis of anthracosis and mediastinal tuberculous lymphadenitis was made and the patients put on antituberculous treatment. But she unfortunately died in the second month of the treatment because of the abdominal complication of gastric adenocarcinoma operation.
    No preview · Article · Feb 2007 · Tuberkuloz ve toraks
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    ABSTRACT: The aims of this study were to investigate the expression of CD10 in normal bladder tissue and urothelial bladder carcinomas and to clarify its association with histopathological variables. A total of 79 urothelial bladder carcinomas were selected from routine archival material. All cases were reevaluated histopathologically and graded according to the World Health Organization (WHO) 1973, WHO/ISUP 1998, and WHO 1999 systems. The TNM system was used for their pathological staging. CD10 immunohistochemical staining was performed in selected slides. Tumoral cases consisted of 74 men (93.7%) and 5 women (6.3%). According to the pathological stage, 25 (31.6%), 33 (41.8%), and 21 (26.6%) cases had pTa, pT1, and pT2-3 carcinomas, respectively. 34 of 79 (43%) urothelial carcinomas and only 1 of 11 (9.1%) nontumoral cases showed positive CD10 immunostaining. It was a cytoplasmic diffuse or granular immunostaining pattern both in nontumoral and tumoral urothelia. There was no statistically significant difference between tumoral and nontumoral cases with respect to CD10 reactivity (p = 0.051), but there was a trend toward significance. In urothelial tumors, there was a significant inverse correlation between pathological stages and CD10 immunoreactivity (p = 0.036, r = -0,237). There was also a statistically significant difference between pTa and pT2-3 urothelial tumors in relation to the CD10 expression (p = 0.034). No association was detected between CD10 expression and grades according to all systems used (p > 0.05). According to our findings, the CD10 expression in noninvasive carcinomas showed a higher level than that in invasive carcinomas, and it is inversely correlated with the pathological stage. CD10 may play an important role in the progression of urothelial bladder carcinomas, and downregulation probably facilitates invasion, especially muscle invasion.
    No preview · Article · Aug 2006 · Urologia Internationalis
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    ABSTRACT: We aim to examine p63 expression in basal cell carcinomas (BCCs) and to investigate association with their histopathological differentiation subtypes. Eighty-four BCCs were classified according to the histopathologic differentiation subtypes. Immunohistochemistry using monoclonal antibody against p63 was performed. In nontumoral skin, p63 expression was consistently seen in basal/suprabasal cells of epidermis, hair matrix cells, and outer root sheath of the hair follicle. In BCCs, the cases were distributed as 47 undifferentiated, 28 differentiated (16 adenoid and 12 keratotic), and nine superficial. The nuclear p63 expression was negative in two cases, whereas 64 BCCs (76.2%) showed homogeneous p63 immunostaining. There was no statistically significant difference between p63 expression and histological differentiation subtypes (p > 0.05). The expression of p63 was found strongly and diffuse in 72.3% of solid undifferentiated and 82.1% differentiated and in 77.8% of superficial type BCCs. p63 is consistently expressed in epidermal basal/suprabasal and adnexal basal cells. Most BCCs have higher homogeneous p63 expression than nontumoral epidermis, which is not changed according to histological differentiation subtypes. Thus, overexpression of p63 in all histological subtypes may confirm that basaloid progenitor cells are linked tumor-cell lineage and have a role in the tumorigenesis of BCC.
    No preview · Article · Apr 2006 · Journal of Cutaneous Pathology
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    ABSTRACT: CD24 is a small, heavily glycosylated cell surface protein, that is expressed in a large variety of solid tumors. It is considered to play an important role in tumor progression and metastasis. We aimed to evaluate CD24 expression in invasive ductal carcinomas (IDCa), ductal carcinoma in situ (DCIS) and non-tumorous breast tissues, and to investigate the relationship between histopathological parameters, estrogen and progesterone receptors, and c-erbB2 expressions. The study included 34 IDCa, 25 DCIS, and 13 non-tumorous breast tissues. All cases were reevaluated histopathologically, and immunohistochemistry was performed with monoclonal CD24 antibody. The results clearly demonstrated that CD24 expression, including membranous and cytoplasmic staining, was significantly higher in DCIS and IDCa than in the non-tumorous breast (p=0.001, p=0.000, and p=0.035, p=0.000, respectively). Cytoplasmic staining was detected predominantly in neoplastic tissues and was significantly increased in high grade DCIS (p=0.013). In invasive carcinomas, although the level of membranous staining was significantly positively correlated with tumor grade (p=0.040), there was no such an association with the cytoplasmic level. However, it showed a trend towards pT (p=0.089). In conclusion, our results suggest that higher CD24 expression may be associated with malignant transformation and progression in breast cancer biology. Furthermore, higher membranous expression and, in particular, cytoplasmic staining seem to predict malignant transformation, and different patterns of CD24 expression may be associated with different pathological features in breast tumors.
    No preview · Article · Feb 2006 · Pathology - Research and Practice