Publications (2)10.18 Total impact
- [Show abstract] [Hide abstract] ABSTRACT: The aim of this study was to determine whether plasma superoxide dismutase (SOD) activity, in comparison with other oxidative parameters, is associated with mortality in humans with septic. We conducted a prospective observational study including 96 patients with septic. Blood samples were collected immediately after study inclusion and 24 hours after. We then determined plasma levels of thiobarbituric acid reactive species, protein carbonyls, SOD, and catalase activities. Plasma carbonyls and SOD activity, but not plasma thiobarbituric acid reactive species and catalase activity, were significantly higher in non-survivors. SOD activity significantly correlated with Acute Physiology and Chronic Health Evaluation II and Multiple Organ Dysfunction Score. In addition, SOD activity presented similar area under the receiver operator characteristic curve when compared with Acute Physiology and Chronic Health Evaluation II to predict mortality. A diminution of 25% or more on SOD activity between D1 and D2 was associated with a better outcome. Our data provide some new information on the use of plasma SOD activity as a biomarker in human sepsis.
- [Show abstract] [Hide abstract] ABSTRACT: To determine xanthine oxidase and superoxide dismutase activities, lipid peroxidation, protein carbonylation, and total radical-trapping antioxidant parameter in survivors and nonsurvivors patients with severe burn injury. Prospective, comparative observational study in an intensive care unit, burn division, in a trauma hospital. Twenty-five consecutive patients who met the established criteria for severe burn injury (total burn surface area of more than 30%). Plasma thiobarbituric acid reactive species and protein carbonyls levels were significantly higher in nonsurvivors than in survivors at 0 and 6 h. Elevated xanthine oxidase activity at 0 h was associated with adverse outcome after burn injury. In contrast, plasma superoxide dismutase activity and total radical-trapping antioxidant parameter did not differ significantly between nonsurvivors and survivors at any time point. For the first time we demonstrate the value of oxidative parameters, namely thiobarbituric acid reactive species, protein carbonyls, and xanthine oxidase activity, in identifying burn patients with a poor prognosis. Whether these parameters are merely markers of clinical course, or whether they signal specific deleterious effects of oxidative stress during the burn injury remains to be elucidated.
Universidade Federal do Rio Grande do Sul
Pôrto de São Francisco dos Casaes, Rio Grande do Sul, Brazil
- Departamento de Bioquímica