Nelly Panté

University of British Columbia - Vancouver, Vancouver, British Columbia, Canada

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Publications (78)473.65 Total impact

  • Nikta Fay · Nelly Panté
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    ABSTRACT: The nuclear import of viral genomes is an important step of the infectious cycle for viruses that replicate in the nucleus of their host cells. Although most viruses use the cellular nuclear import machinery or some components of this machinery, others have developed sophisticated ways to reach the nucleus. Some of these have been known for some time; however, recent studies have changed our understanding of how some non-enveloped DNA viruses access the nucleus. For example, parvoviruses enter the nucleus through small disruptions of the nuclear membranes and nuclear lamina, and adenovirus tugs at the nuclear pore complex, using kinesin-1, to disassemble their capsids and deliver viral proteins and genomes into the nucleus. Here we review recent findings of the nuclear import strategies of three small non-enveloped DNA viruses, including adenovirus, parvovirus, and the polyomavirus simian virus 40. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · Jun 2015 · Current Opinion in Virology
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    ABSTRACT: Gp78, an ERAD-associated E3 ubiquitin ligase, localizes to mitochondria-associated ER and targets the mitofusin (Mfn1/Mfn2) mitochondrial fusion proteins for degradation. Gp78 is also the cell surface receptor for autocrine motility factor (AMF) that prevents Gp78-dependent mitofusin degradation. Gp78 ubiquitin ligase activity promotes ER-mitochondria association and ER-mitochondria calcium coupling, processes that are reversed by AMF. Electron microscopy of HT-1080 fibrosarcoma cancer cells identified both smooth (∼8 nm) and wider (∼50-60 nm) rough ER-mitochondria contacts. Gp78 shRNA knockdown and AMF treatment selectively reduced the extent of rough ER-mitochondria contacts without impacting smooth ER-mitochondria contacts. Concomitant siRNA knockdown of Mfn1 increased smooth ER-mitochondria contacts in both control and shGp78 cells while knockdown of Mfn2 increased rough ER-mitochondria contacts selectively in shGp78 HT-1080 cells. The mitofusins therefore inhibit ER-mitochondria interaction. Regulation of close ER-mitochondria contacts by Mfn1 and of rough ER-mitochondria contacts by AMF-sensitive Gp78 degradation of Mfn2 define novel mechanisms that regulate ER-mitochondria interactions. © 2015. Published by The Company of Biologists Ltd.
    No preview · Article · Jun 2015 · Journal of Cell Science
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    Nikta Fay · Nelly Panté
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    ABSTRACT: DNA viruses undertake their replication within the cell nucleus, and therefore they must first deliver their genome into the nucleus of their host cells. Thus, trafficking across the nuclear envelope is at the basis of DNA virus infections. Nuclear transport of molecules with diameters up to 39 nm is a tightly regulated process that occurs through the nuclear pore complex (NPC). Due to the enormous diversity of virus size and structure, each virus has developed its own strategy for entering the nucleus of their host cells, with no two strategies alike. For example, baculoviruses target their DNA-containing capsid to the NPC and subsequently enter the nucleus intact, while the hepatitis B virus capsid crosses the NPC but disassembles at the nuclear side of the NPC. For other viruses such as herpes simplex virus and adenovirus, although both dock at the NPC, they have each developed a distinct mechanism for the subsequent delivery of their genome into the nucleus. Remarkably, other DNA viruses, such as parvoviruses and human papillomaviruses, access the nucleus through an NPC-independent mechanism. This review discusses our current understanding of the mechanisms used by DNA viruses to deliver their genome into the nucleus, and further presents the experimental evidence for such mechanisms.
    Preview · Article · May 2015 · Frontiers in Microbiology
  • Pierre O Garcin · Nelly Panté
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    ABSTRACT: The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epithelial-mesenchymal transition through polyomavirus middle T antigen transformation in transgenic mice. By a combination of immunofluorescence and electron microscopy, we found that MVMp endocytosis occurs at the leading edge of migrating cells in proximity to focal adhesion sites. By using drug inhibitors of various endocytic pathways together with immunofluorescence microscopy and flow cytometry analysis, we discovered that MVMp can use a number of endocytic pathways, depending on the host cell type. At least three different mechanisms were identified: clathrin-, caveolin-, and clathrin-independent carrier-mediated endocytosis, with the latter occurring in transformed cells but not in LA9 fibroblasts. Copyright © 2015 Elsevier Inc. All rights reserved.
    No preview · Article · Apr 2015 · Virology
  • Pierre O Garcin · Ivan R Nabi · Nelly Panté
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    ABSTRACT: Galectin-3 has previously been found to be required by the parvovirus minute virus of mice prototype strain (MVMp) for infection of mouse fibroblast cells. Since MVMp is an oncotropic virus, and galectin-3 is a multifunctional protein implicated in cancer metastasis, we hypothesized that galectin-3 and Mgat5, the Golgi enzyme that synthesizes high-affinity glycan ligands of galectin-3, might play a role in MVMp infection. Using siRNA-mediated knockdown of galectin-3 in mouse cells transformed with polyomavirus middle T antigen and Mgat5(-/-) mouse mammary tumor cells, we found that galectin-3 and Mgat5 are both necessary for efficient MVMp cell entry and infection, but not for cell binding. Moreover, we found that human cancer cells expressing higher levels of galectin-3 were more efficiently infected with MVMp than cell lines expressing lower galectin-3 levels. We conclude that galectin-3 and Mgat5 are involved in MVMp infection, and propose that galectin-3 is a determinant of MVMp oncotropism. Copyright © 2015 Elsevier Inc. All rights reserved.
    No preview · Article · Mar 2015 · Virology
  • Nelly Panté · Birthe Fahrenkrog
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    ABSTRACT: Xenopus oocytes are large in size and perfectly suited for microinjection experiments. Their nuclei, which can be readily isolated manually, are characterized by an extremely high density of nuclear pore complexes (NPCs). Therefore, Xenopus oocytes are an excellent system to study NPC structure and molecular architecture, as well as nucleocytoplasmic transport on an ultrastructural level. A wide range of electron microscopy (EM) techniques can be employed to do so and thin-sectioning immuno-EM has been proven to be a powerful tool in this context. NPCs are composed of multiple copies of a set of about 30 different nucleoporins, which are often large, multidomain proteins. Their complex organization within NPCs can be unraveled by using domain-specific antibodies to individual nucleoporins in combination with microinjection and expression of epitope-tagged nucleoporins. Here, we describe the immuno-EM methods using Xenopus oocyte that allow for precise ultrastructural localization of nucleoporins within the structure of the NPC.
    No preview · Article · Dec 2014 · Methods in cell biology
  • Pierre O. Garcin · Nelly Panté
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    ABSTRACT: The parvovirus minute virus of mice, prototype strain (MVMp), preferentially infects and kills cancer cells. This intrinsic MVMp oncotropism may depend in part on the early stages of MVMp infection. To test this hypothesis, we investigated the early events of MVMp infection in mouse LA9 fibroblasts and a highly invasive mouse mammary tumor cell line derived from polyomavirus middle T antigen-mediated transformation. Using a combination of fluorescence and electron microscopy, we found that various parameters of the cell migration process affect MVMp infection. We show that, after binding to the plasma membrane, MVMp particles rapidly cluster at the leading edge of migrating cells, which exhibit higher levels of MVMp uptake than non-motile cells. Moreover, promoting cell migration on a fibronectin matrix increased MVMp infection, and induction of epithelial–mesenchymal transition allowed MVMp replication in non-permissive epithelial cells. Hence, we propose that cell migration influences the early stages of MVMp infection.
    No preview · Article · Nov 2014 · Virology
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    Full-text · Article · Jan 2014 · Biophysical Journal
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    ABSTRACT: Disassembly of the nuclear lamina is essential in mitosis and apoptosis requiring multiple coordinated enzymatic activities in nucleus and cytoplasm. Activation and coordination of the different activities is poorly understood and moreover complicated as some factors translocate between cytoplasm and nucleus in preparatory phases. Here we used the ability of parvoviruses to induce nuclear membrane breakdown to understand the triggers of key mitotic enzymes. Nuclear envelope disintegration was shown upon infection, microinjection but also upon their application to permeabilized cells. The latter technique also showed that nuclear envelope disintegration was independent upon soluble cytoplasmic factors. Using time-lapse microscopy, we observed that nuclear disassembly exhibited mitosis-like kinetics and occurred suddenly, implying a catastrophic event irrespective of cell- or type of parvovirus used. Analyzing the order of the processes allowed us to propose a model starting with direct binding of parvoviruses to distinct proteins of the nuclear pore causing structural rearrangement of the parvoviruses. The resulting exposure of domains comprising amphipathic helices was required for nuclear envelope disintegration, which comprised disruption of inner and outer nuclear membrane as shown by electron microscopy. Consistent with Ca(++) efflux from the lumen between inner and outer nuclear membrane we found that Ca(++) was essential for nuclear disassembly by activating PKC. PKC activation then triggered activation of cdk-2, which became further activated by caspase-3. Collectively our study shows a unique interaction of a virus with the nuclear envelope, provides evidence that a nuclear pool of executing enzymes is sufficient for nuclear disassembly in quiescent cells, and demonstrates that nuclear disassembly can be uncoupled from initial phases of mitosis.
    Full-text · Article · Oct 2013 · PLoS Pathogens
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    Nikta Fay · Nelly Panté
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    ABSTRACT: Intermediate filaments (IFs) have recently been shown to serve novel roles during infection by many viruses. Here we have begun to study the role of IFs during the early steps of infection by the parvovirus minute virus of mice (MVM). We found that during early infection with MVM, after endosomal escape, the vimentin IF network was considerably altered, yielding collapsed immunofluorescence staining near the nuclear periphery. Furthermore, we found that vimentin plays an important role in the life cycle of MVM. The number of cells, which successfully replicated MVM, was reduced in infected cells in which the vimentin network was genetically or pharmacologically modified; viral endocytosis, however, remained unaltered. Perinuclear accumulation of MVM-containing vesicles was reduced in cells lacking vimentin. Our data suggests that vimentin is required for the MVM life cycle, presenting possibly a dual role: (1) following MVM escape from endosomes and (2) during endosomal trafficking of MVM.
    Preview · Article · Jul 2013 · Virology
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    Shelly Au · Wei Wu · Nelly Panté
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    ABSTRACT: Baculoviruses are one of the largest viruses that replicate in the nucleus of their host cells. During infection, the rod-shape, 250-nm long nucleocapsid delivers its genome into the nucleus. Electron microscopy evidence suggests that baculoviruses, specifically the Alphabaculoviruses (nucleopolyhedroviruses) and the Betabaculoviruses (granuloviruses), have evolved two very distinct modes for doing this. Here we review historical and current experimental results of baculovirus nuclear import studies, with an emphasis on electron microscopy studies employing the prototypical baculovirus Autographa californica multiple nucleopolyhedrovirus infecting cultured cells. We also discuss the implications of recent studies towards theories of nuclear transport mechanisms.
    Full-text · Article · Jul 2013 · Viruses
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    ABSTRACT: Cellular factors associated with the parvovirus minute virus of mice (MVM) during infection are thought to play important roles in the MVM life cycle but only a few of these have been identified. Here we used a proteomic-based approach in order to identify host-binding partners of MVM. Using purified MVM as bait for immunoprecipitation assays, a total of 150 proteins were identified in MVM immunoprecipitates by quantitative liquid chromatography-tandem mass spectrometry. Galectin-3 was one of six proteins showing a statistically significant enrichment across replicates. Small interfering RNA depletion studies revealed an important role for Galectin-3 in MVM endocytosis and infectivity in LA9 mouse fibroblast cells. Galectin-3-depleted cells were less susceptible to MVM infection than control cells and showed a significant reduction of MVM cellular uptake, but not of MVM binding to the cell surface. Our results indicate an important role for Galectin-3 in the cellular uptake of MVM. We propose that Galectin-3 facilitates the access of MVM to its receptor(s) at the plasma membrane and in this way promotes MVM endocytosis.
    No preview · Article · Dec 2012 · Journal of proteomics
  • Sarah Cohen · Igor Etingov · Nelly Panté
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    ABSTRACT: The nuclear envelope (NE) is a vital structure that separates the nucleus from the cytoplasm. Because the NE is such a critical cellular barrier, many viral pathogens have evolved to modulate its permeability. They do this either by breaching the NE or by disrupting the integrity and functionality of the nuclear pore complex (NPC). Viruses modulate NE permeability for different reasons. Some viruses disrupt NE to deliver the viral genome into the nucleus for replication, while others cause NE disruption during nuclear egress of newly assembled capsids. Yet, other viruses modulate NE permeability and affect the compartmentalization of host proteins or block the nuclear transport of host proteins involved in the host antiviral response. Recent scientific advances demonstrated that other viruses use proteins of the NPC for viral assembly or disassembly. Here we review the ways in which various viruses affect NE and NPC during infection.
    No preview · Article · Sep 2012 · International review of cell and molecular biology
  • Wei Wu · Nelly Pante
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    ABSTRACT: Background: Influenza A virus poses a serious threat to world public health, particularly the pandemic H1N1 and avian H5N1. Currently, the major anti-influenza strategies are vaccination and antiviral drugs. However, both of these approaches target structural components of the virus which undergo mutation and become resistant to these antiviral approaches. An alternative way to inhibit influenza infection is to interfere with the viral entry into the nucleus, since this process is necessary for viral replication. So far, there are at least two stretches of amino acids sequences located on influenza nucleoprotein (NP) that are know to mediate the nuclear import of the viral ribonucleoproteins (vRNPs) containing the viral genome: NLS1 at the N-terminus of NP, and NLS2 spanning residues 198-216. Sequence allignment shows both NLSs on NP are highly conserved between different strains of influenza A, suggesting that NLSs are ideal candidate for novel antiviral approaches. Methods: To assess this, we are first defining the contribution of the NLSs of NP to nuclear import. The functional role of NLS1 has been very well characterized in previous studies. The specific role of the NLS2 is, however, ill-defined. In our study, we generated chimeric protein by fusing NLS1 or NLS2 to a heterologous protein and characterize the contribution of these NLSs to nuclear import. To analyzed the contribution of the NLS2’s basic amino acids to the nuclear import of the chimera protein, we did site-directed mutagenesis towards NLS2 in chimeric protein. Further analyzation of NLSs's antiviral function was performed by competition assay. Results: Our results showed that NLS2 renders a weak nuclear import behavior compared to NLS1. Interestingly, with only one basic amino acid difference (lysine to arginine) the NLS2 of seasonal flu (H1N1 and H5N1) contributes stronger to the nuclear import of the chimera protein than the NLS2 from pandemic H1N1 and avian H5N1. Using site-directed mutagenesis we further analyzed the contribution of the NLS2’s basic amino acids to the nuclear import of the chimera protein. Our results suggest that NLS2 from pandemic H1N1 and avian H5N1 function as a classical bipartite NLS. However, NLS2 from seasonal flu (H1N1 and H5N1) behaves as a monopartite NLS instead of a bipartite NLS. By performing competition assays using NLS1 and NLS2 chimeras in infected cells, we found that the competing NLSs were able to successfully delayed infection of influenza A virus. Conclusions: NLS2 showed strain-dependent function in nuclear import and contributed less comparing to NLS1. Competition assay results clearly indicate that a good strategy to employ in the development of new influenza antiviral drugs is to interfere with the function of NLSs of influenza NP.
    No preview · Conference Paper · Feb 2012
  • Shelly Au · Nelly Panté
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    ABSTRACT: Baculoviruses are one of the largest viruses that replicate in the nucleus of their host cells. During an infection the capsid, containing the DNA viral genome, is released into the cytoplasm and delivers the genome into the nucleus by a mechanism that is largely unknown. Here, we used capsids of the baculovirus Autographa californica multiple nucleopolyhedrovirus in combination with electron microscopy and discovered this capsid crosses the NPC and enters into the nucleus intact, where it releases its genome. To better illustrate the existence of this capsid through the NPC in its native conformation, we reconstructed the nuclear import event using electron tomography. In addition, using different experimental conditions, we were able to visualize the intact capsid interacting with NPC cytoplasmic filaments, as an initial docking site, and midway through the NPC. Our data suggests the NPC central channel undergoes large-scale rearrangements to allow translocation of the intact 250-nm long baculovirus capsid. We discuss our results in the light of the hypothetical models of NPC function.
    No preview · Article · Nov 2011 · Journal of Structural Biology
  • Anne Monette · Nelly Panté · Andrew J Mouland
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    ABSTRACT: A hallmark of HIV type 1 and other lentiviruses is their ability to infect and replicate in nondividing cells by commandeering host nuclear transport factors. During the early stages of infection, this is expected to permit the safe passage of viral preintegration complexes (PICs) through nuclear pores. Numerous nuclear transport factors have been identified as essential for HIV-1 infection by genome-wide small interfering RNA screens, and many of these are currently under investigation. Here, using knockdown studies, Matreyek and Engelman further characterize the importance of transportin-3 and nuclear pore complex component nucleoporin 153 for the early stages of HIV-1 infection and show that these two proteins operate synergistically. Also, as was previously observed for transportin-3, they show that the requirement of nucleoporin 153 for PIC nuclear entry is determined by the HIV-1 Capsid protein. The refinement of the list of key nuclear pore complex and transport proteins required for PIC entry, along with a better understanding of the specific mechanisms employed, will undoubtedly lead to the development of future antiretroviral therapies that will have the potential to block HIV-1 viral DNA integration.
    No preview · Article · Nov 2011 · Future Microbiology
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    ABSTRACT: Protozoan parasites, such as Leishmania, still pose an enormous public health problem in many countries throughout the world. Current measures are outdated and have some associated drug resistance, prompting the search into novel therapies. Several innovative approaches are under investigation, including the utilization of host defence peptides (HDPs) as emerging anti-parasitic therapies. HDPs are characterised by their small size, amphipathic nature and cationicity, which induce permeabilization of cell membranes, whilst modulating the immune response of the host. Recently, members of the cathelicidin family of HDPs have demonstrated significant antimicrobial activities against various parasites including Leishmania. The cathelicidin bovine myeloid antimicrobial peptide 28 (BMAP-28) has broad antimicrobial activities and confers protection in animal models of bacterial infection or sepsis. We tested the effectiveness of the use of BMAP-28 and two of its isomers the D-amino acid form (D-BMAP-28) and the retro-inverso form (RI-BMAP-28), as anti-leishmanial agents against the promastigote and amastigote intracellular Leishmania major lifecycle stages. An MTS viability assay was utilized to show the potent antiparasitic activity of BMAP-28 and its protease resistant isomers against L. major promastigotes in vitro. Cell membrane permeability assays, caspase 3/7, Tunel assays and morphologic studies suggested that this was a late stage apoptotic cell death with early osmotic cell lysis caused by the antimicrobial peptides. Furthermore, BMAP-28 and its isomers demonstrated anti-leishmanial activities against intracellular amastigotes within a macrophage infection model. Interestingly, D-BMAP-28 appears to be the most potent antiparasitic of the three isomers against wild type L. major promastigotes and amastigotes. These exciting results suggest that BMAP-28 and its protease resistant isomers have significant therapeutic potential as novel anti-leishmanials.
    Full-text · Article · May 2011 · PLoS Neglected Tropical Diseases
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    Anne Monette · Nelly Panté · Andrew J Mouland
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    ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) commandeers host cell proteins and machineries for its replication. Our earlier work showed that HIV-1 induced the cytoplasmic retention of nucleocytoplasmic shuttling and ribonucleic acid (RNA)-binding proteins. This retention is dependent on nuclear export of the viral genomic RNA and on changes in the localization and expression level of the nucleoporin (Nup) p62 (Nup62). To further characterize the extent of perturbation induced by HIV-1, we performed proteomics analyses of nuclear envelopes (NEs) isolated from infected T cells. Infection induced extensive changes in the composition of the NE and its associated proteins, including a remarkable decrease in the abundance of Nups. Immunogold electron microscopy revealed the translocation of Nups into the cytoplasm. Nup62 was identified as a component of purified virus, and small interfering RNA depletion studies revealed an important role for this Nup in virus gene expression and infectivity. This detailed analysis highlights the profound effects on NE composition induced by HIV-1 infection, providing further evidence of the magnitude of viral control over the cell biology of its host.
    Preview · Article · May 2011 · The Journal of Cell Biology
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    ABSTRACT: Parvoviruses are small, nonenveloped, single-stranded DNA viruses which replicate in the nucleus of the host cell. We have previously found that early during infection the parvovirus minute virus of mice (MVM) causes small, transient disruptions of the nuclear envelope (NE). We have now investigated the mechanism used by MVM to disrupt the NE. Here we show that the viral phospholipase A2, the only known enzymatic domain on the parvovirus capsid, is not involved in causing NE disruption. Instead, the virus utilizes host cell caspases, which are proteases involved in causing NE breakdown during apoptosis, to facilitate these nuclear membrane disruptions. Studies with pharmacological inhibitors indicate that caspase-3 in particular is involved. A caspase-3 inhibitor prevents nuclear lamin cleavage and NE disruption in MVM-infected mouse fibroblast cells and reduces nuclear entry of MVM capsids and viral gene expression. Caspase-3 is, however, not activated above basal levels in MVM-infected cells, and other aspects of apoptosis are not triggered during early MVM infection. Instead, basally active caspase-3 is relocalized to the nuclei of infected cells. We propose that NE disruption involving caspases plays a role in (i) parvovirus entry into the nucleus and (ii) alteration of the compartmentalization of host proteins in a way that is favorable for the virus.
    Full-text · Article · Mar 2011 · Journal of Virology
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    Sarah Cohen · Shelly Au · Nelly Panté
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    ABSTRACT: Many viruses depend on nuclear proteins for replication. Therefore, their viral genome must enter the nucleus of the host cell. In this review we briefly summarize the principles of nucleocytoplasmic transport, and then describe the diverse strategies used by viruses to deliver their genomes into the host nucleus. Some of the emerging mechanisms include: (1) nuclear entry during mitosis, when the nuclear envelope is disassembled, (2) viral genome release in the cytoplasm followed by entry of the genome through the nuclear pore complex (NPC), (3) capsid docking at the cytoplasmic side of the NPC, followed by genome release, (4) nuclear entry of intact capsids through the NPC, followed by genome release, and (5) nuclear entry via virus-induced disruption of the nuclear envelope. Which mechanism a particular virus uses depends on the size and structure of the virus, as well as the cellular cues used by the virus to trigger capsid disassembly and genome release. This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import.
    Full-text · Article · Dec 2010 · Biochimica et Biophysica Acta

Publication Stats

5k Citations
473.65 Total Impact Points


  • 2000-2015
    • University of British Columbia - Vancouver
      • Department of Zoology
      Vancouver, British Columbia, Canada
    • The University of Calgary
      Calgary, Alberta, Canada
    • Eawag: Das Wasserforschungs-Institut des ETH-Bereichs
      Duebendorf, Zurich, Switzerland
  • 2002
    • Justus-Liebig-Universität Gießen
      Gieben, Hesse, Germany
  • 1999
    • ETH Zurich
      • Institute of Biochemistry
      Zürich, ZH, Switzerland
  • 1998
    • Universität Heidelberg
      Heidelburg, Baden-Württemberg, Germany
  • 1994-1998
    • Universität Basel
      • Department of Biophysical Chemistry
      Bâle, Basel-City, Switzerland
  • 1995
    • Kyushu University
      • Graduate School of Medical Sciences
      Hukuoka, Fukuoka, Japan
    • The Scripps Research Institute
      • Department of Cell and Molecular Biology
      لا هویا, California, United States