E Maran

Università degli Studi di Trieste, Trst, Friuli Venezia Giulia, Italy

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Publications (5)10.52 Total impact

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    ABSTRACT: Cytotoxic effects of aldicarb, its sulfone and sulfoxide, and propoxur, lipid peroxidation and antioxidant parameters in Chinese Hamster Ovary (CHO-K1) cells were determined. D,L-buthionine-(S,R)-sulfoximine (BSO) was assayed to determine the role of GSH in the protection against carbamate cytotoxicity. Pre-treatment with 60 microM BSO, induced a significant decrease in the glutathione reductase (GR; 64-141%), the glutathione peroxidase (GPx; 10-30%) and the glutathione S-transferase (GST; 59-93%) activities, and its GSH levels (79-85%), while the oxidized glutathione (GSSG) levels significantly increased (64-78%) respect to experiment non-BSO-pretreated. Carbamates BSO pre-treated vs. non-BSO pre-treated showed a significant increase in malondialdehyde (MDA) production (from 13% to 52% vs. 25% to 93%). These data suggest that carbamates could injure CHO-K1 cells via oxidative stress by the increase of MDA production; moreover, BSO enhance the oxidative damage caused by carbamates. However, the glutathione system protects cells from carbamates damage.
    No preview · Article · Mar 2010 · Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association
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    ABSTRACT: The effect of four carbamates, aldicarb and its metabolites (aldicarb sulfone and aldicarb sulfoxide) and propoxur on glutathione content and the activity of the enzymes involved in the sulfur-redox cycle in the mammalian cellular model CHO-K1 cells after 24-h exposure were determined. Carbamate exposure resulted in a depletion of intracellular reduced glutathione (GSH) content, no change was observed in oxidized glutathione (GSSG) and a decrease in GSH/GSSG ratio was detected. After carbamates exposition a GSH/GSSG decreases in ranged from 12.44% to 21.35% of control was observed. Depletion of GSH levels was accompanied by the induction of glutathione reductase (GR) after 24h exposure with each of the four carbamates to CHO-K1 cells. After aldicarb sulfone, aldicarb sulfoxide, and propoxur exposure, glutathione peroxidase (GPx) activity increased in CHO-K1 cells by 198%, 32%, and 228% of control, respectively. After aldicarb sulfone and propoxur exposure, glutathione transferase (GST) activities increased by 49% and 230% of control, respectively. Due to the role played by GSH in preventing cytotoxicity via free-radical scavenging, results obtained suggest that high concentrations of aldicarb sulfone and propoxur closely resembling oxidative stress in CHO-K1 cells.
    No preview · Article · Apr 2008 · Ecotoxicology and Environmental Safety
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    Full-text · Conference Paper · Jan 2007
  • M. J. Ruiz · E. Maran · H. Berrada · M. Fernández

    No preview · Article · Sep 2006 · Toxicology Letters
  • E Maran · M Novic · P Barbieri · J Zupan
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    ABSTRACT: The present study focuses on fish antibiotics which are an important group of pharmaceuticals used in fish farming to treat infections and, until recently, most of them have been exposed to the environment with very little attention. Information about the environmental behaviour and the description of the environmental fate of medical substances are difficult or expensive to obtain. The experimental information in terms of properties is reported when available, in other cases, it is estimated by standard tools as those provided by the United States Environmental Protection Agency EPISuite software and by custom quantitative structure-activity relationship (QSAR) applications. In this study, a QSAR screening of 15 fish antibiotics and 132 xenobiotic molecules was performed with two aims: (i) to develop a model for the estimation of octanol--water partition coefficient (logP) and (ii) to estimate the relative binding affinity to oestrogen receptor (log RBA) using a model constructed on the activities of 132 xenobiotic compounds. The custom models are based on constitutional, topological, electrostatic and quantum chemical descriptors computed by the CODESSA software. Kohonen neural networks (self organising maps) were used to study similarity between the considered chemicals while counter-propagation artificial neural networks were used to estimate the properties.
    No preview · Article · Oct 2004 · SAR and QSAR in Environmental Research