Publications (2)3.13 Total impact
- [Show abstract] [Hide abstract] ABSTRACT: The unsaturated polyphosphoester (UPPE) polymer is being investigated as an injectable and biodegradable system for alveolar bone repair in the treatment of periodontal diseases. The incorporation of beta-tricalcium phosphate (beta-TCP) particles into the UPPE polymer was previously shown to significantly increase the material's mechanical properties. Moreover, in vitro experiments demonstrated that the UPPE/beta-TCP composite was capable of zero-order release of tetracycline for over 2 weeks. In this study, we investigated the in vitro cytotoxicity of each individual component, the resulting cross-linked network and the degradation products of the UPPE/beta-TCP composite using an AlamarBlue viability assay. We confirmed that each individual component except beta-TCP and the in vitro degradation products of the composite displayed a dose-dependent cytotoxic response. Once cross-linked, however, the composite did not demonstrate an adverse response. Our results suggest that the UPPE/beta-TCP composite holds great promise for use as an injectable and biodegradable alveolar bone substitute.
- [Show abstract] [Hide abstract] ABSTRACT: The aim of this study was to investigate the in vitro cytotoxicity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer (0.01-10 mg/mL) for 24 h. The L-929 mouse fibroblasts were then exposed to the treated cell culture medium for 24 h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL (P>0.05). The two evaluation methods showed no significant differences (P>0.05). This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications.
Tongji HospitalWu-han-shih, Hubei, China