[Show abstract][Hide abstract] ABSTRACT: The role of mitochondria in Adriamycin (ADR) -induced heart failure and the protective effects of Sini Decoction (SND) were investigated.
SD rats were randomly divided into three groups, control group, heart failure group (model group) and SND group. ADR was injected in the rats of heart failure group and SND group by caudal vein. After injection, the rats in SND group were given SND (3.75/kg/d, p. o.). Three weeks later, cardiac function, mitochondrial swelling, content of malondialdehyde (MDA), activity of Mn SOD, Na+-K+ ATPase and Ca2+ ATPase were measured. The mRNA expression of Mn SOD was also detected by RT-PCR.
Compared with model group, the cardiac function, the activity and the mRNA expression of Mn SOD and the activity of Na+-K+ ATPase and Ca2+ ATPase were significantly elevated, while the degree of mitochondria swelling and the content of MDA were reduced in SND-treated rats.
The data suggests that oxidative stress is present in the mitochondria of myocardium in ADR-induced heart failure rats and it can be reduced by SND. The mechanism may be closely related to the protective effects on mitochondria.
No preview · Article · Jul 2005 · Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
[Show abstract][Hide abstract] ABSTRACT: To investigate protective effects of Sini decoction on cardiomyocytes from oxidative stress damage induced by peroxide (H2O2).
Ventricular cardiomyocytes were prepared from 1-to-2-day-old Sprague-Dawley rats and cultured with DMEM culture media containing 15% neonatal bovine serum. 4 days later, culture media were replaced with neonatal bovine serum-free DMEM with 15%, 1.5% and 0.15% SD rats serum containing Sini decoction, respectively. Viablity percentage of rat cardiomyocytes was detected by flow cytometry using Annexin V-FITC to screen the appropriate SD rat serum concentration containing Sini decoction in DMEM culture media. Cardiomyocytes were exposed to 1 mmol/L H2O2 for 0.5 h, 1 h, 2 h, 4 h and 8 h respectively. MDA and SOD in cardiomyocytes were measured. Apoptosis and necrosis percentage of cardiomyocytes were measured by flow cytometry using Annexin V-FITC as well. Bcl-xL and Bcl-xS mRNA in cardiomyocytes were detected by polymerase chain reaction.
15% SD rat serum with Sini decoction in DMEM culture midia was the appropriate concentrate for cell growth. MDA and SOD in cardiomyocytes were changed with time elapse of cardiomyocytes exposure to H2O2. Sini decoction could inhibit rat cardiomyocytes from oxidative stress damage induced by H2O2.
To some extend, Sini decoction inhibit rat cardiomyocytes from oxidative stress damage induced by peroxide through promoting expression of Bcl-xL mRNA and decreasing expression of Bcl-xS mRNA in cardiomyocytes.
No preview · Article · Jun 2005 · Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials