Xiaofan Yuan

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (18)38.92 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25°C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%.
    No preview · Article · Nov 2015 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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    ABSTRACT: An efficient ultrasound-assisted aqueous two-phase extraction and enrichment process for phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems was developed in this work. An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system due to its fine partitioning and recycling behaviors. Single-factor experiments and response surface methodology were used to optimize the process parameters of the ultrasound-assisted aqueous two-phase extraction. The optimal conditions were as follows: a salt concentration of 23.5%, an ethanol concentration of 20%, an extraction time of 37 min, an extraction temperature of 30°C, a liquid/solid ratio of 30:1 w/w, and an ultrasound power of 300 W. Under the above conditions, the extraction yields of echinacoside and acteoside (the main components of phenylethanoid glycosides) reached 5.35 and 6.22 mg/g dry material weight, respectively. The contents of echinacoside and acteoside in the extracts reached 27.56 and 30.23 mg/g, respectively, which were 2.46- and 2.58-fold higher than the amounts obtained in ultrasound-assisted extraction. In conclusion, ultrasound-assisted aqueous two-phase extraction was an efficient, ecofriendly and economical method, and may be a promising technique for extracting and enriching bioactive components from plants.This article is protected by copyright. All rights reserved
    No preview · Article · Jan 2015 · Journal of Separation Science
  • Hui Zhou · Xiaofan Yuan · Qingsheng Zhao · Bing Zhao · Xiaodong Wang
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    ABSTRACT: Microencapsulation was a widely used technique for the unstable materials because the wall prevented the core from contacting with oxygen. To improve the stability of crocetin and maximize the protection afforded by the wall, in the present study, microencapsulation was applied for crocetin by spray-drying the first time with three wall materials (beta-cyclodextrin, gum arabic and maltodextrin). The oxygen transmission barrier was proposed to evaluate the potential gas barrier of these wall materials, and it was measured by the deterioration kinetics of crocetin. To enhance the oxygen transmission barrier of the wall, the major conditions of spray-drying and the storage were investigated. Results showed that the deterioration rate followed the first kinetic model closely. Spray-drying was applicable to microencapsulating crocetin with the microencapsulation efficiency of 77.91–85.03 % under the inlet air temperature 210 °C. The oxygen pressure in the microcapsule was reduced to <0.114 ± 0.005 atm for all the three wall materials. The oxygen transmission barrier of the wall microencapsulated by the three materials was qualified by thermodynamic parameter activation energy (29.436 ± 0.080, 58.813 ± 0.312 and 49.376 ± 0.125 kJ/mol, respectively). Gum arabic was the most suitable agent for microencapsulating crocetin, and a dry environment was preferred for its storage because the low relative humidity was beneficial to the stable structure of the microcapsule. This study would be helpful to the industrial application of crocetin.
    No preview · Article · Oct 2013 · European Food Research and Technology
  • Boyan Liu · Jie Ouyang · Xiaofan Yuan · Liwei Wang · Bing Zhao
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    ABSTRACT: A simple and efficient chromatographic method for large-scale preparative separation of phenylethanoid glycosides (mainly contain echinacoside and acteoside) from Cistanche deserticola was developed. The adsorption properties of eight macroporous resins were evaluated. Three selected resins were further screened depending on the adsorption kinetics curves, in which HPD300 resin showed the best separation efficiency. The adsorption isotherm data on HPD300 resin were fitted to the Freundlich equation in certain concentration range. The dynamic adsorption and desorption experiments were carried out on columns packed with HPD300 resin to optimize the separation process. The breakthrough curves showed that acteoside had a higher affinity to the resin than echinacoside. The contents of echinacoside and acteoside in the product increased from 1.79% and 1.43% in the crude extracts to 16.66% and 15.17%, with recovery yields of 80.41% and 90.17%, respectively. The purity of total phenylethanoid glycosides in the product was 76.58%.
    No preview · Article · Aug 2013 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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    ABSTRACT: Ultrasonic extraction (USE) of polysaccharide from cordyceps cephalosporium mycelia was introduced and compared with hot water extraction. Crude polysaccharides were investigated for morphological and thermodynamic properties. Two major fractions (USEP40-1, USEP70-1) were purified and investigated for IR spectra, molecular weights, monosaccharide compositions and antioxidant activities. The results suggested that USE gained higher extraction efficiency due to its intense extraction process. USE caused a more compact arrangement in crude polysaccharides. IR spectra of USEP40-1 and USEP70-1 were different at around 1746 cm(-1). The molecular weights of USEP40-1 and USEP70-1 were 61.4 kDa and 25.1 kDa. USEP40-1 and USEP70-1 were composed of D-mannose, D-glucose, D-galactose, L-rhamnose, and L-arabinose with molar ratio of 11.52:5.54:8.75:2.45:2.59 and 11.50:6.74:5.75:4.46:2.39, respectively. Different ABTS and hydroxyl radical scavenging activities were observed in USEP40-1 and USEP70-1.
    Full-text · Article · May 2012 · International journal of biological macromolecules
  • Jian Sun · Jie Xiao · Xiaodong Wang · Xiaofan Yuan · Bing Zhao
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    ABSTRACT: A hairy root culture system of Calotropis gigantea was established and effects of mechanical wounding (MW) and elicitors [methyl jasmonate (MJ), yeast extract (YE) and chitosan (CS)] on cardenolide production were investigated. All treatments stimulated the production of cardenolide in hairy root cultures of C. gigantea. CS was the most effective elicitor, followed by MJ. YE and MW also improved cardenolide yield in individual treatments. The highest cardenolide yield (1,050 ± 55 mg/l) was obtained after adding 50 mg CS/l for 20 days, which was 2.7-fold higher than the control.
    No preview · Article · Nov 2011 · Biotechnology Letters
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    ABSTRACT: Polysaccharides from cultivated Saussurea involucrata (CSIP) were purified, two major fractions (CSIP1-2 and CSIP2-3) were investigated for their molecular weights, monosaccharide compositions and in vitro antioxidant activities. The results suggested that the molecular weights of CSIP1-2 and CSIP2-3 were approximately 163.5 kDa and 88.6 kDa, respectively. CSIP1-2 was composed of glucose, galactose, xylose, rhamnose, arabinose and galacturonic acid with a molar ratio of 1.651:0.39:0.062:8.331:1.759:40.426. CSIP2-3 was composed of glucose, galactose, xylose, rhamnose, arabinose and galacturonic acid with a molar ratio of 0.762:0.657:0.112:5.587:0.318:44.655. Different scavenging activities on superoxide radical, DPPH radical and hydroxyl radical were observed in CSIP1-2 and CSIP2-3 at tested concentrations.
    Full-text · Article · Nov 2011 · International journal of biological macromolecules
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    ABSTRACT: Polysaccharides were extracted from Asparagus officinalis. A novel ultrasonic circulating extraction (UCE) technology was applied for the polysaccharide extraction. Three-factor-three-level Box-Behnken design was employed to optimize ultrasonic power, extraction time and the liquid-solid ratio to obtain a high polysaccharide yield. The optimal extraction conditions were as follows: ultrasonic power was 600 W, extraction time was 46 min, the liquid-solid ratio was 35 mL/g. Under these conditions, the experimental yield of polysaccharides was 3.134%, which was agreed closely to the predicted value. The average molecular weight of A. officinalis polysaccharide was about 6.18×10(4) Da. The polysaccharides were composed of glucose, fucose, arabinose, galactose and rhamnose in a ratio of 2.18:1.86:1.50:0.98:1.53. Compared with hot water extraction (HWE), UCE showed time-saving, higher yield and no influence on the structure of asparagus polysaccharides. The results indicated that ultrasonic circulating extraction technology could be an effective and advisable technique for the large scale production of plant polysaccharides.
    No preview · Article · Aug 2011 · International journal of biological macromolecules
  • Liwei Wang · Xiaodong Wang · Xiaofan Yuan · Bing Zhao
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    ABSTRACT: Asparagus officinalis L. has several biological activities including antifungal, antiviral and antitumoral activities due to the steroidal saponins. Normally diosgenin and sarsasapogenin are analysed separately by thin-layer chromatography or high-performance liquid chromatography (HPLC-UV or HPLC-ELSD), which is time-consuming and expensive, so we need to find a rapid solution to this problem. To develop a sensitive, rapid and validated TLC method for simultaneous detection and quantification of diosgenin and sarsasapogenin. Samples were prepared by extraction of A. officinalis with 70% aqueous ethanol to get steroidal saponins, and then hydrolysed using 36  mL 2 m hydrochloric acid for 3  h. The hydrolysis product was extracted with chloroform, and then analysed by TLC, the results of which were verified by HPLC and HPLC-MS. The retention factor (R(f)) of diosgenin and sarsasapogenin on TLC plate were 0.49 and 0.6, respectively. After calculation from the regression equation of the standard curve, the contents of diosgenin and sarsasapogenin in the A. officinalis extract were 0.27-0.46 and 0.11-0.32%, respectively. The study showed that thin-layer chromatography can be applied for the determination of diosgenin and sarsasapogenin in the oldest tissue of A. officinalis, and also can be conducted for screening of sapogenin in other plant or extracts.
    No preview · Article · Jan 2011 · Phytochemical Analysis
  • Chunming Xu · Bing Zhao · Yuan Ou · Xiaodong Wang · Xiaofan Yuan · Yuchun Wang
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    ABSTRACT: Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag+) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5% (v/v) yeast extract, 0.2gl−1 chitosan and 75μM Ag+ at the 15th day of the cell culture. The highest syringin production reached 741.9mgl−1, which was 3.6−fold that of the control. The glucose−6-phosphate dehydrogenase (EC, phenylalanine ammonia lyase (EC and peroxidase (EC activities increased significantly after elicitor treatment. The maximum enzyme activities were obtained when the treatment time was 6h.
    No preview · Article · Jun 2007 · World Journal of Microbiology and Biotechnology
  • Feng Ge · Xiaofan Yuan · Xiaodong Wang · Bing Zhao · Yuchun Wang
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    ABSTRACT: Arnebia euchroma was grown in a 2-l periodically submerged, airlift bioreactor (PSAB) in which the non-submerged (immobilization culture) and submerged (suspension culture) operations were controlled automatically. PSAB had advantages in improving cell growth, shikonin content, shikonin production and cell aggregation compared with suspension culture. Under the optimal submerged/non-submerged period of 10 min/15 h, the shikonin content (4.6%, w/w) and, cell dry mass (16.8 g/l) were 229 and 26% higher than those in suspension culture.
    No preview · Article · May 2006 · Biotechnology Letters
  • Xiaofan Yuan · Bing Zhao · Yuchun Wang
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    ABSTRACT: Cell culture of Saussurea medusa in a 2-l periodically submerged air-lift bioreactor (PSAB) was investigated. The periodically unsubmerged (immobilization culture) and submerged (suspension culture) operation of the whole bioreactor system was controlled by the electromagnetic valves and driven rod controller connected with the time controller. The maximum cell biomass (10.4 g l−1), content of total flavonoids (4.8%), production of total flavonoids (501 mg l−1) and the optimal size distribution of cell aggregates were obtained under the optimal unsubmerged/submerged period of 10 min/8 h (the cell inoculation size was 1.0 g l−1, and the air flowrate was 20/40 l h−1). Though the content of total flavonoids was still a little lower than that obtained from the shake flask culture, the specific growth rate of cells in PSAB was 2.1-fold and the production of total flavonoids was 1.7-fold of those in shake flasks. It was concluded that PSAB had advantages in improving cell growth of S. medusa and the production of total flavonoids.
    No preview · Article · Nov 2004 · Biochemical Engineering Journal
  • Shu-an Chen · Bing Zhao · Xiaodong Wang · Xiaofan Yuan · Yuchun Wang
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    ABSTRACT: La3+ and Ce3+, either singly or a mixture, promoted crocin production of Crocus sativus callus but Nd3+ had little effect and all metal ions were toxic above 100 microM. La3+ (60 microM) promoted growth of callus significantly but increased crocin only slightly. Ce3+ (40 microM) significantly promoted crocin production but had little effect on cell growth. La3+ (60 microM) and Ce3+ (20 microM) together gave the highest dry weight biomass (20.4 g l(-1)), crocin content (4.4 mg g(-1)) and crocin production (90 mg l(-1)) which were, respectively, 1.7-fold, 4.2-fold and 7.1-fold of those without additions. Nd3+ (40 microM) only slightly promoted cell growth and crocin production.
    No preview · Article · Feb 2004 · Biotechnology Letters
  • Shu-an Chen · Xiaodong Wang · Bing Zhao · Xiaofan Yuan · Yuchun Wang
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    ABSTRACT: Saffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate (300 mg l(-1)) at 22 degrees C in dark with naphthalene acetic acid (2 mg l(-1)) and 6-benzyladenine (1 mg l(-1)) to give maximum biomass (16 g dry wt l(-1)), and with indole 3-acetic acid (2 mg l(-1)) and 6-benzyladenine (0.5 mg l(-1)) for crocin formation. The maximum crocin production (0.43 g l(-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method.
    No preview · Article · Sep 2003 · Biotechnology Letters
  • Jie Ouyang · Xiaodong Wang · Bing Zhao · Xiaofan Yuan · Yuchun Wang
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    ABSTRACT: The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30 day's culture, 0.02 mmoll(-1) MRE gave the highest content (20.8%) and production (1.6 gl(-1)) of PeG, which were 104 and 167% higher than those obtained in control (without rare earth elements).
    No preview · Article · May 2003 · Journal of Biotechnology
  • An Chenshu · Xiaodong Wang · Xiaofan Yuan · Bing Zhao · Yuchun Wang
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    ABSTRACT: Cryopreservation of callus tissue of Artimisia annua L. was optimized. Two lines of calli were precultured on MS medium with 5% (v/v) dimethyl sulfoxide, and protected by a cryoprotectant containing 15% (v/v) ethylene glycol, 15% (v/v) dimethyl sulfoxide, 30% (v/v) glycerol and 13.6% (w/v) sucrose. The highest survival rate of callus A201 reached 87% after it was pretreated at 25 degrees C, cryopreserved by liquid nitrogen, recovered in water bath at 25 degrees C and reloaded at 25 degrees C with 34% (w/v) sucrose solution, and that of callus A202 reached 78% after it was treated as callus A201, except pretreated at 35 degrees C, recovered at 35 degrees C and reloaded with 47.8% (w/v) sucrose solution.
    No preview · Article · Feb 2003 · Biotechnology Letters
  • Xiaofan Yuan · Qian Wang · Bing Zhao · Yuchun Wang
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    ABSTRACT: Callus cultures of Saussurea medusa were cultivated on solid culture medium supplemented with either Ce3+, La3+, Nd3+ or a mixture of rare earth elements. Ce3+, 0.05 mM, gave the highest biomass (0.53 g dry wt per flask) and total flavonoids (27.5 mg per flask), which were, 70% and 100% higher than those without Ce3+ addition, respectively. Ce3+, 0.01–0.1 mM, or La3+, 0.05 mM, or the mixture of rare earth elements, 0.025–0.1 mM, can substitute for 6-benzyladenine, and 0.025 mM Ce3+ can partly substitute for naphthaleneacetic acid in promoting cell growth and biosynthesis of total flavonoids in S. medusa.
    No preview · Article · Jan 2002 · Biotechnology Letters
  • Yuchun Wang · Haoxian Zhang · Bing Zhao · Xiaofan Yuan
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    ABSTRACT: Hairy root cultures of Artemisia annua L were cultivated for 30 days under either white, red, blue, yellow or green light. Red light at 660 nm gave the highest biomass of hairy roots (5.73 g dry wt cells l–1 medium) and artemisinin content (31 mg arteminsinin g–1 dry cells) which were, respectively, 17% and 67% higher than those obtained under white light.
    No preview · Article · Nov 2001 · Biotechnology Letters