Vincenzo Monteverde

Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Sicily, Italy

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Publications (13)6.06 Total impact

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    ABSTRACT: The aim of this study was to investigate the effect of omega-3 supplementation on some haematological parameters like red blood cells (RBC), haemoglobin (Hgb), haematocrit (Hct), white blood cells (WBC), Neutrophils (Neu) and Lymphocytes (Lym) that may have a direct effect on the performance of athletic horses. Ten regularly trained Standardbred horses (6 geldings and 4 females, 4-5 years old, mean body weight 500 ± 25 kg) were used for the study. They were randomly divided into two groups. Experimental group received an omega-3 dietary supplementation every day for 30 days. No supplementation received the control group. Every 10 days, horses took part at 1660 meters harness race and blood samples were collected from each horse: one in the morning before race (pre) and one after race (post). The application of one way analysis of variance for repeated mesures (ANOVA) showed a statistically significant difference due to the race in the two different groups. The results obtained in the present study show a discordant effect of supplementation with omega 3 on RBC, HCT and Hgb , while omega-3 supplementation have been shown to have a better effect on WBC , Neu and Lym, but further studies should be performed to better evaluate the benefits of these on the performance of the athletic horse.
    No preview · Article · Jan 2015
  • V. Monteverde · M. Rizzo · D. Alberghina · F. Fazio · E. Giudice · G. Piccione

    No preview · Article · Jan 2015
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    ABSTRACT: Twenty clinically healthy goats were divided into two equal groups based on two different diets. Goats in group A were fed with 25 % barley, 12 % oat, 15 % faba bean, 10 % pea, 20 % sugar beet pulps, 5 % molasses and 3 % mineral. Goats in group B were fed with 25 % corn, 15 % soybean hulls, 12 % soybean meal, 10 % sunflower meal, 20 % sugar beet pulps, 5 % molasses and 3 % mineral. To establish the effect of diet composition on haematological and biochemical concentrations, blood samples were collected from the external jugular vein once a week, for 1 month (T0–T4), twice daily (0700 and 1800 hours). Multivariate analysis of variance (MANOVA) showed a significant effect of different diets (P < 0.01) and periods (P < 0.001) on serum total protein and urea concentrations. Also, a significant effect of time of day (P < 0.001) was observed on urea serum concentration. In conclusion, different diet composition had an influence on serum total protein and urea concentrations. A diet with 25 % corn induce an increase of serum total protein concentration and a decrease of serum urea concentration compared with a diet with 25 % barley.
    No preview · Article · Sep 2014 · Comparative Clinical Pathology
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    ABSTRACT: The aim of the study was to investigate clock gene expression in Bos taurus and the alteration of that during two pathological conditions, evaluating the daily expression pattern of four clock genes (Per2, Cry2, Bmal1, Clock) in peripheral blood cells. Five healthy cows, five affected by Brucellosis (BR) and five affected by Bovine Viral Diarrhoea-Mucosal Disease (BVD-MD) were housed in indoor stalls under natural spring conditions, blood samples were collected at 4 h intervals over a 24 h period. Statistical analysis showed rhythmic expression of clock genes mRNAs in healthy cows. Cows affected by BR did not show any rhythmic expression of clock genes mRNAs, cows affected by BDV mRNA levels of Bmal1, Clock and Cry2 changed during the day. These findings highlighted that circadian system could be involved in homeostasis alteration and that clock genes could be considerate as regulatory genes or early response genes during inflammation, so, their regulation should be evaluated in health research and treatment.
    No preview · Article · Jul 2014 · Journal of applied biomedicine
  • G. Piccione · F. Arfuso · V. Monteverde · I. Vazzana · A. Zumbo · C. Faggio
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    ABSTRACT: Introduction - The neonatal period represents a critical stage in development of physiological functions. During this phase morphological, physiological and behavioral changes take place in newborn animals to permit a successful adaptation to the extra-uterine environment. Moreover, newborn lambs have limited energy reserves and need a rapid access to colostrum to maintain homeothermy and survive. In addition to energy, colostrum provides proteins, including immunoglobulins, which ensure passive systemic immunity. Therefore, getting early access to the udder is essential for the neonate. Aim - The purpose of this study was to monitor rectal temperature and the course of some metabolic parameters (serum total proteins, albumin, total cholesterol, triglycerides, creatinine and urea) of ten newborn lambs (Comisana breed) during the first 30 days of life in order to obtain useful information for neonatal care. Material and methods - For each animal rectal temperature was recorded using a digital thermometer (HI92704, Hanna Instruments Bedfordshire, UK) with the probe being inserted to a depth of 9 cm. From all animals blood samples were collected by jugular venipuncture at the same hour (9:00) every 3 days for 30 days starting from birth. By means of an UV Spectrophotometer, the serum concentration of total proteins, albumin, total cholesterol, triglycerides, creatinine and urea were determined. One-way repeated measures analysis of variance (ANOVA), followed by Bonferroni's Test, was used to determine statistically significant differences. Results and discussion - Data analysis of variance showed a statistical effect of days of life on total proteins (P<0.0003), albumin, total cholesterol, triglycerides (P<0.0001) and urea (P=0.0002), during the first 30 days of life, while no statistical significant effect of days of life on creatinine (P=0.09) and rectal temperature (P=0.06) is showed during the experimental period. Conclusion - The results obtained in this study make a contribution to the knowledge of physiological and metabolic modification in lambs during the first 30 days of life and give useful information for the diagnosis and treatment of neonatal diseases.
    No preview · Article · Jun 2013 · Large Animal Review
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    ABSTRACT: The aim of this study was to investigate the effects of seasonal variations in Mediterranean area on haematological parameters in ten dairy cows (Bruna breed) kept under natural photoperiod and temperature. On blood samples, collected through an external jugular venipuncture every 30 days for 12 months, haematological profile was performed using an automatic multiparametric analyser for haematology. Red blood cell (RBC), white blood cell, haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets (PLT) and plateletcrit (PCT) were measured. One-way for repeated measure analysis of variance showed a statistically significant effect of time of year (P < 0.05) on RBC, Hb, Hct, PLT and PCT. The majority of the haematological values obtained, comparable to the values considered to be normal for dairy cows, showed that seasonal variations in haematological parameters are related to changes in physical environment with particular emphasis on ambient temperature, relative humidity and temperature–humidity index. These parameters need further investigations in relation to age and sex differences with particular attention to reproductive status.
    No preview · Article · Jul 2012 · Comparative Clinical Pathology
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    ABSTRACT: The circadian rhythms have evolved in domestic animals in more different breeding management, and reflect an adaptive mechanism to react in advance to the regular environmental changes. The aim of this research was to evaluate the circadian rhythm of haematological parameters in cows and to study if Brucella abortus infection in cows can alterate their rhythmicity. For this purpose, five healthy cows and five Brucella infected cows were enrolled. Blood samples were collected in spring (sunrise 06:30, sunset 19.00), every 4 h over a 24 h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, and a complete blood cell count with differential was performed. One-way ANOVA for repeated measures was applied to evaluate the effect of time on haematological parameters. Using cosinor rhythmometry, four rhythmic parameters were determined: mesor, amplitude, acrophase and robustness. One-way repeated measures Analysis of Variance were applied to evaluate a significant effect of time of day on each parameter. P values <0.05 were considered statistically significant. The application of one-way ANOVA showed a statistical significant effect of time of day on haematocrit (PCV) and haemoglobin (Hb) in healthy cows. In Brucella-infected cows, statistical significant changes of neutrophils, eosinophils and lymphocytes were observed. Using Cosi-nor method, the same parameters showed a daily rhythmicity. Our results showed that a daily rhythmicity exists both for healthy and infected cows, but in different haematological parameters. In particular, hemoglobin, and hematocrit in and neutrophils, eosinophils and lymphocytes in. This study is a preliminary observation in changes of circadian clocks during pathological events in domestic animals. In particular, further investigations should be conducted to evaluate how the molecular clock components are influenced not only by an inflammatory response but contemporarily by a change in L/D cycles.
    No preview · Article · Apr 2012 · Large Animal Review
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    ABSTRACT: The existence of daily rhythmicity of serum total proteins, their fractions and some biochemical parameters in healthy cows (group A) and in cows affected by Brucella abortus (group B) was assessed in order to clarify the role of circadian timing processes in healthy and disease in cows. On each subject, blood samples were collected, every 4 h over a 24-h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, via intravenous cannulas inserted into the jugular vein. On all samples, serum total proteins and their fractions (α1-, α2-, β1-, β2- and γ-globulins), gammaglutamyl transferase (GGT), alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine and urea were assessed. The application of one-way analysis of variance showed a significant effect of time of day in group A only on creatinine and urea. In group B, a significant effect of the time of day was observed on total proteins, albumin, β-globulins, GGT, creatinine and urea. Daily rhythmicity was exhibited by creatinine and urea in healthy cow. Whereas, daily rhythmicity of total proteins, albumin, β-globulins, GGT, creatinine and urea was observed in cows affected by B. abortus. Our results improve the knowledge about changes of circadian system during pathological events in dairy cow.
    No preview · Article · Mar 2012 · Comparative Clinical Pathology
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    ABSTRACT: In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfully detected Leptospira in four dubious cases of human leptospirosis from archival tissue specimens and one leptospirosis-positive canine specimen. A sensitive method for leptospirosis identification in FFPE tissues would be a useful tool to screen histological specimen archives and gain a better assessment of human leptospirosis prevalence, especially in tropical countries, where large outbreaks can occur following the rainy season.
    Full-text · Article · Feb 2012 · Memórias do Instituto Oswaldo Cruz
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    ABSTRACT: The aim of this study was to evaluate haematological and haematochemical responses to training and competition in standardbred horses. We investigated the modifications that occur in athletic horses during different workloads of training and after official competitions of 1,600 and 2,000m. The study was carried out on 15 gelding clinically healthy horses. The animals were divided into three groups: group A, group B, and group C. All animals of group A were subjected to two training schedules with different workloads. Group B took part in a 1,600m trot official competition, and group C took part in a 2,000m trot official competition. Blood samples were collected from each animal at rest, after training/competition, and 60min after the end of the physical exercise. On each subject of groups A, B, and C, red blood cell, white blood cell, haemoglobin, haematocrit, and platelets were recorded by means of a multiparametric automatic analyzer, and the concentrations of triglycerides, glucose, creatinine, urea, glutamate pyruvate transaminases, and glutamate oxaloacetate transaminases were assessed with a spectrophotometer. Two-way repeated measures analysis of variance was used to determine significant differences. The results indicated that exercise had a significant effect on haematological and haematochemical parameters, the effect being proportional to the workload.
    No preview · Article · Feb 2010 · Comparative Clinical Pathology
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    ABSTRACT: Spirocerca lupi (Rudolphi 1809) is a cosmopolitan nematode of dogs and wild carnivores. In the past it has been reported in Italy, mainly in southern regions and in Sicily, where the parasite was observed in foxes in 2005. The parasite typically produces nodular masses in the oesophagus and thoracic aorta. During the 2003-2004 hunting season, the authors investigated a total of 55 foxes (Vulpes vulpes) hunted or killed by car accidents in the provinces of Palermo and Agrigento. All the foxes were subjected to necropsy and 6 (9.16%) had S. lupi nodules located exclusively in the gastric wall. The nature of the nodules was determined by opening them and detecting the nematodes inside, which were identified as S. lupi. Some of the nodules were characterized anatomopathologically and histopathologically. The formation of the parasitic nodule in the stomach only suggests a deviation from the route commonly followed by the nematode to reach the oesophagus, the elective anatomical site for completion of its lifecycle. This survey gives a contribution to the epidemiology of this parasite which is severely outdated in Italy and highlights some distinctive features of the life cycle and parasite migration.
    Full-text · Article · Jan 2010 · Polish journal of veterinary sciences
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    ABSTRACT: Piccione, G., Casella, S., Giannetto, C., Monteverde, V. and Ferrantelli, V. 2009. Exercise-induced modifications on haematochemical and electrophoretic parameters during 1600 and 2000 meters trot races in Standardbred horses. J. Appl. Anim. Res., 35: 131–135.The aim of the present study was to evaluate the modifications of some haematochemical and electrophoretic parameters during two different workloads in Standardbred horses. Ten clinically healthy horses were divided into two equal groups, taking part in an official trot race at 1600 or 2000 meters course, respectively. Blood samples were collected from each horse at rest, after warm up, 0, 30 and 60 min after the end of the race. Serum concentrations of albumin, globulins, total proteins, total cholesterol, triglycerides, glucose, hydroxybutyrate, urea, creatinine, creatine kinase (CK), glutamate pyruvate transaminases (GPT) and glutamate oxalacetate transaminases (GOT) were assessed. Exercise increased triglyceride, glucose, creatinine and CK and decreased GOT which tended to reach rest level 60 min after exercise. Globulins were affected by exercise but there was no set pattern.
    No preview · Article · Jun 2009 · Journal of Applied Animal Research
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    Giuseppe Piccione · S Casella · V. Monteverde · C Giannetto · G Caola
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    ABSTRACT: The authors studied the changes of some haematological parameters during two different workloads in Standardbred horses. Ten horses, clinically healthy, were divided into two groups of five subjects each. The two groups of Standardbred (Group A and Group B) had been separately and specifically trained to take part in the official 1600 and 2000 meters trot races, respectively. On each subject of Group A and Group B, red blood cell (RBC), white blood cell (WBC), platelets (PLT), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were recorded. Blood samples were collected from each horse at rest, after warm up, before racing, immediately after racing, 30 and 60 min after the end of the race. Twoway repeated measures analysis of variance (ANOVA) was used to determine significant differences. The statistical analysis, the ANOVA followed by the Bonferroni&apos;s test, showed that during the experimental period, modifications observed for some haematological parameters are not related to the intensity and the type of exercise; only platelet reactivity is altered by different workload. Our results further confirm the data demonstrated previously.
    Full-text · Article · Jan 2008 · Acta veterinaria