[Show abstract][Hide abstract] ABSTRACT: Lysates of erythrocytes and sera of 52 patients with osteoarthrosis (OA) and 30 healthy subjects were used used to determine adenosine deaminidase (ADA, AMP deaminase (AMPDA) and adenine deaminidase (AD). Their activities were unrelated to the age and sex of the patients. At admission, patients with OA showed enhanced activity of ADA in sera and reduced activity of AMPDA and AD in lysates compared with normal values. These changes depended on clinical features of OA and were especially pronounced in patients with poly-OA and synovitis. These data suggest participation of enzymes of the adenyl branch of purine metabolism in pathogenesis of OA. Treatment of hospitalized patients allowed to achieve positive dynamics of the above activities coupled to their improved clinical conditions even though enzymatic activity of erythrocyte lysates remained different from that in healthy subjects. It is concluded that enzymatic assays used in the study may be used as additional diagnostic methods for the assessment of synovitis and optimizatic of control over efficiency of OA therapy.
No preview · Article · Jan 2010 · Klinicheskaia meditsina
[Show abstract][Hide abstract] ABSTRACT: To evaluate effectiveness of a natural mineral bishofit in combined treatment of rheumatoid arthritis (RA).
The study included 71 RA patients with minimal activity of the pathological process and articular disorders of stage II. Bishofit was used in baths and compresses. 33 AR patients of group 1 took bishofit baths (14 procedures) and non-steroid anti-inflammatory drugs (NSAID), 27 RA patients of group 2 were treated with bishofit compresses (14 procesures) and NSAID, 11 patients of group 3 (controls) received NSAID alone. The effect of the treatment was assessed on the basis of clinical data, the length of the affected joint circumference, antitryptic blood activity, activity of malate dehydrogenase, lactate dehydrogenase isoenzymes, plasmin, plasminogen, ESR, sialic acids.
Bishofit-treated patients showed earlier (by 5-6 days) and greater (by 25-35%) decrease of the pain, joint and inflammatory indices as well as duration of morning stiffness, length of the affected joint circumference and joint mobility, improvement of the tests' results. A response and an appreciable response to combined therapy with bishofit were achieved in 62.9-63.6% of cases vs 45.5% in the control group. Bishofit baths and compresses did not differ considerably by therapeutic efficacy.
No preview · Article · Feb 2003 · Terapevticheskii arkhiv
[Show abstract][Hide abstract] ABSTRACT: To characterize purine metabolism in osteoarthritis (OA) and gout arthritis (GA) diagnosis and differential diagnosis of these diseases.
We estimated xanthine oxidase (XA), xanthine dehydrogenase (XDG), 5'-nucleotidase (5'-NT) activity, esoenzymes of XDG and content of uric acid (UA) in the sera of 44 patients with osteoarthritis and 34 patients with gout arthritis.
Hyperuricemia was revealed in 25 percent of patients with osteoarthritis, in 64.7 percent of patients with gout arthritis. XO, XDG, 5'NT activity, XO/XDG activities ratio, XDG-2 esoenzymes and content of UA were increased in OA patients compared to healthy controls. XO, XDG activity, XDG-2 esoenzymes and UA content in GA patients were higher than those in OA patients.
The enzyme difference found may promote differential diagnosis of OA and GA. The enzyme indices essentially depend on clinical specificity of the disease and can be helpful in the assessment of the treatment efficiency.
No preview · Article · Feb 2000 · Terapevticheskii arkhiv
[Show abstract][Hide abstract] ABSTRACT: Bishofit baths and compresses on affected joints were added to combined treatment of 43 patients with dystrophic metabolic arthritis (DMA). Twenty control DMA subjects received. nonsteroid antiflammatory drugs without bishofit. Comparison of the two groups demonstrated that adjuvant bishofit promoted more rapid clinical remission of DMA. The baths versus compresses yielded essentially similar results.
No preview · Article · Jul 1991 · Klinicheskaia meditsina
[Show abstract][Hide abstract] ABSTRACT: Creatine kinase (CK) activity was assessed in the direct and reverse tests and CK isoenzymes in agar gel for 41 patients with systemic lupus erythematosus (SLE). With growing activity of the process, there was a decline of CK activity in the reverse test though in the direct test this activity was scarcely changeable allowing a shift to predominance of the direct response. Significant differences were found in CK reverse reaction which correlated with SLE activity and pattern. The more active the disease, the less content of CK-1 and CK-3 isoenzymes. Investigations of CK activity and isoenzymes during SLE treatment help objectivize the clinical control.
No preview · Article · Feb 1991 · Sovetskaia meditsina