[Show abstract][Hide abstract] ABSTRACT: We analysed using electrophoresis the total genomic DNA extracted from isolated Auerbach plexus ganglia of the hypertrophic duodenum upstream from a partial experimental stenosis. Results indicated the presence of two extra-bands migrating below the high molecular weight DNA. suggesting that DNA amplification is the basic mechanism of the DNA neosynthesis previously observed in myenteric neurons.
No preview · Article · Nov 1995 · International Journal of Developmental Neuroscience
[Show abstract][Hide abstract] ABSTRACT: Hypertrophy of the smooth muscle wall of the rat small intestine occurs in the loops upstream from a partial surgical stenosis. This phenomenon is due both to cell hypertrophy and cell proliferation (hyperplasia). The thickness of the muscular layers reached its maximum after ten days from the intervention and then was maintained over the following days. Hyperplasia of the smooth muscle tissue has been demonstrated by means of autoradiography after administration of tritiated-thymidine. This investigation revealed the presence of 37%-44.5% of heavily labeled smooth muscle nuclei, index of active DNA neo-synthesis. On the contrary, no labeled nuclei were observed in the downstream tract as well as in the small intestine of the controls. These results, together with those obtained by other researchers, confirms that the smooth muscle tissue can undergo cell proliferation under particular conditions and that this phenomenon is often associated with the hypertrophic process.
No preview · Article · Feb 1992 · Bollettino della Società italiana di biologia sperimentale