Kh Ayed

Hôpital Charles-Nicolle, Tunis-Ville, Tūnis, Tunisia

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Publications (2)1.46 Total impact

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    ABSTRACT: Polymorphism of the gene encoding components of the renin-angiotensin-aldosterone synthase system (RAAS) represents an area of intense research of cardiovascular disease associations. Numerous studies have addressed the role of RAAS gene polymorphisms in the development and progression of renal disease. Also, it has been reported that patient with ACE (DD) and angiotensinogen AGT (TT) genotypes are associated with chronic allograft dysfunction (CAD). We investigated the effects of gene polymorphisms of the renin-angiotensin-aldosterone system in renal transplant patients (81 males and 50 females; mean age 29.6+/-10.2 years). Genotypes were determined using polymerase chain reaction sequence specific primers and PCR followed by RFLP analysis. Renal allograft recipients with chronic allograft dysfunction had significantly higher frequencies of the MM genotype than those without CAD (P<0.05). The other genetic polymorphisms of the RAAS were not associated with CAD. This study proves that determination of AGT M235T genotype before transplantation may help identify patients who are at risk for chronic renal transplant dysfunction.
    No preview · Article · Apr 2006 · Transplant Immunology
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    ABSTRACT: Late loss of kidney allograft, caused by immunological and non-immunological factors, remains a major problem in the field of transplantation. The aim of this study was to analyze the risk factors affecting long-term kidney graft survival more than 15 years. In a retro-spective analysis, clinical and laboratory variables and outcome of 330 patients who received a kidney transplant from living or cadaveric ABO-compatible donors at the Charles Nicolle Hospital, Tunis between 1986 and 2005 were recorded. A total of 58 patients who had follow-up data longer than 15 years constituted the subjects of this study. Patients were classified into two groups: Group I (Gp I), those who had kidney graft survival more than 15 years and Group II (Gp II), those who had kidney graft survival less than 15 years. There were 27 patients in Gp I (46.5%) and 31 in Gp II (53.5%). Graft loss in Gp II patients occurred in the first year in 15.1%, at three years in 35.5 %, at five years in 71 % and at 10 years in 83.9 %. The cause of graft loss was chronic graft dysfunction in 24 cases (77.4%), recurrence of the original kidney disease in three and graft versus host disease, urinary fistula, vascular rejection and graft rupture in one case each. There was no statistically significant difference between recipient and donor age or sex, duration on dialysis, number of acute rejections and infections between the two groups. Eleven of 27 patients (40.7 %) in Gp I and eight of 31 patients (25.8 %) in Gp II received total mismatched kidneys while the others received kidneys with varying degrees of match. The HLA DR2 matching was higher in Gp I (44.4 %) than in Gp II (29 %), whereas DR3 matching was higher in Gp II (45.2 %) in comparison with Gp I (11.1 %). Cross-match was negative in all our patients. Thirteen patients (48.1%) in Gp I and 17 (54.8 %) in Gp II.had a history of having episode(s) of acute rejection The number of acute rejection episodes did not contribute significantly to long-term graft survival in our series. Delayed graft function significantly lowered long-term graft survival; it was seen in seven cases in Gp I (25.9 %) versus 23 cases in Gp II (74.2 %) (X2=13.46). In our study, the long-term graft survival was similar to what is reported from developed countries. The main risk factors were HLA DR matching and delayed graft function.
    No preview · Article · Apr 2006 · Saudi journal of kidney diseases and transplantation: an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia
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    ABSTRACT: Nasopharyngeal cancer (NPC) is an epidermoid-cell lineage carcinoma of the head and neck region with unusually variable incidence rates throughout the world. The ethiology of NPC is multifactorial and includes virological, genetic and environmental factors. A genetic component to NPC has been suggested by association of the malignancy with HLA system. Using serologic and molecular methods, 45 NPC patients were typed for HLA class I and class II and were compared to one hundred unrelated normal Tunisian controls. Our results showed that the antigen frequency of HLA B13, DRB1* 03 and DRB1* 15 were found significantly higher in the NPC compared to controls group (15,5% VS 4%, 26,4 VS, 11.5%, 14,4% VS 6,5% respectively). Moreover, we observed that HLA-DRB1* 11 was less frequent among the patients than in controls and HLA A23 was absent in patients with NPC, but was present in 18% of normal controls suggesting a protective association with NPC. This study show a clear association of HLA class I and class II with NPC in Tunisian patients.
    No preview · Article · Jan 2001
  • S. Jendoubi · Kh. Ayed · Y. Gorgi · F. Sassi
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    ABSTRACT: Systemic lupus erythematosis (SLE) is a multifactorial disease with both genetic and enviromental etiology. Increased risk of SLE has been described in association with some HLA markers and the complement C4A null allele. Genetic susceptibility to SLE may vary amongst different populations. Few data exist on associations of HLA class II and class III alleles of the major Histocompatibility (MHC) and susceptibility to SLE in Tunisians. We have examined HLA DRB1, DQB1 and C4 allotypes in 54 Tunisian SLE patients and 100 matched controls. Results : HLA DRB1* 0301 DRB1*1501 and C4AQO alleles were each increased in the SLE patients, while the frequencies of HLA DRB1*04 and DQB1*03 were decreased. HLA DQB1* 0201 and DQB1* 0602 were slightly more frequent in the SLE patients compared to controls but did not achieve statistical signifiance (39% vs 27.9% and 22. 1% vs 15.2% respectively) three of sixteen SLE patients with HLA DR1* 1501 were associated with HLA DQB1*0501 rather DQB1*0602 as it has been reported in European SLE patients. In addition MHC class II alleles (DRB1, DQB1) and C4 null associations that have been noted in other ethnic groups are also found in Tunisian, suggesting shared susceptibility factors accross ethnic lines in predisposition to SLE.
    No preview · Article · Jan 2001