Ken-ichi Hisamatsu

University of Yamanashi, Kōhu, Yamanashi, Japan

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Publications (63)50.36 Total impact

  • Ken-ichi Hisamatsu · Itsuhiro Kudo · Kiyoshi Makiyama

    No preview · Article · Nov 2015
  • Ken-ichi Hisamatsu · Itsuhiro Kudou · Tomoyuki Takane · Kiyoshi Makiyama
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    ABSTRACT: Objectives and Methods: Twenty four severe obstructive sleep apnea syndrome (OSAS) patients, 35 sleep disordered breathing (SDB) patients with excessive daytime sleepiness, and 74 simple snorers underwent coblation-assisted uvulopalatopharyngoplasty (cobUPPP) under local anesthesia. Polysomnography (PSG) events, snoring, QOL (SF-36 v2) and excessive daytime sleepiness on the Epworth sleepiness scale (ESS) were assessed 3 months after the operation. The overall assessment criteria of the operative effectiveness on OSAS were proposed using subjective symptoms and PSG events. Informed consent was obtained in writing from all participants prior to cobUPPP. Results: AI (P<0.0001), AHI (P<0.0001), ODI (P<0.0001), minimum SpO2 (P=0.028), SpO2<90% (P=0.008) and the arousal index (P=0.001) were significantly ameliorated. Excessive daytime sleepiness was ameliorated in 34/35 cases and bothering snoring was improved, based on visual analog scale scoring, in 54/55 cases. Treatment of OSAS was assessed as highly effective in 11/24, 9/24 effective and 4/24 ineffective. Transient discomfort of the pharynx was an occasional complaint. Conclusion: cobUPPP was effective for severe OSAS without significant complications and could be very useful to manage excessive daytime sleepiness and socially unacceptable snoring.
    No preview · Article · Jan 2014 · Practica Otologica
  • Ken-ichi Hisamatsu · Kiyoshi Makiyama · Ryoji Hirai

    No preview · Article · Jan 2009
  • Ken-ichi Hisamatsu · Hajime Inoue · Kiyoshi Makiyama · Masami Homma
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    ABSTRACT: Our purpose was to investigate mucosal cell injury due to the nitric oxide (NO)-superoxide system in otitis media with effusion. We determined the levels of nitrotyrosine (NT) and NO and the activities of superoxide dismutase (SOD) and lactic dehydrogenase (LDH) in 90 middle ear fluid samples. The NT concentration was significantly higher in group A (<16 years old) than in group C (>50 years old; p < .05), and significantly higher in the acute group than in the chronic group (p < .05). The NO concentration did not show a significant difference among the groups. The activity of SOD showed significant correlations with the concentrations of NT and NO and with LDH activity (p < .05). The LDH activity was significantly greater in group A than in group C (p < .05). Our results indicate involvement of the NO-superoxide system in the pathogenesis of otitis media with effusion, showing evidence of protein and/or cell injury in the middle ear.
    No preview · Article · Oct 2005 · The Annals of otology, rhinology, and laryngology

  • No preview · Article · Jan 2003
  • Hajime Inoue · Ken-ichi Hisamatsu · Kazumasa Ando · Ryo Ajisaka · Norio Kumagai
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    ABSTRACT: A gas mediator, nitric oxide is converted to peroxynitrite in the presence of superoxide anion. Peroxynitrite is a potent oxidant, which injures various tissues and organs by nitration of the tyrosine residues of proteins, and it enhances the late response of inflammation. The determination of nitrated tyrosine, nitrotyrosine, which is a stable final metabolite of peroxynitrite, provides an important indicator of tissue disorders caused by peroxynitrite. This paper reports a competitive solid-phase immunoassay for measuring nitrotyrosine in various biological specimens. In this study, peroxidase-conjugated nitrotyrosine was prepared by reaction of nitrotyrosine with 1,4-benzoquinone treatment, and then it was allowed to compete with nitrotyrosine on an anti-nitrotyrosine antibody-coated 96-well multiplate. No amino acids or related compounds tested in the experiments interfered with the immune reaction of nitrotyrosine, except cysteine, which only slightly inhibited the immune reaction at the concentrations higher than 1000 times the concentration of nitrotyrosine. The limit of detection of free nitrotyrosine was approximately 500 pg/mL (2 nM) at a competition ratio (B/B(o)%) of 80%. The newly developed enzyme immunoassay (EIA) method was used for assay of nitrotyrosine in biological specimens, with the following results: (i) Lipopolysaccharide (LPS) activation of RAW264.7 cells induced a significant increase in nitrotyrosine production compared to that with nonactivated cells. N(omega)-nitro-L-arginine methyl ester decreased nitrotyrosine production with either LPS-activated or nonactivated RAW cells. There is a relationship between nitrotyrosine production and nitrite ion. (ii) The nitrotyrosine level detected in the plasma specimens from healthy volunteers was 35.21 +/- 4.87 ng/mL (135.4 +/- 18.7 nM). (iii) The concentration of nitrotyrosine in the nasal lavage fluid of allergic rhinitis patients was 41.40 +/- 20.96 ng/mL (159.02 +/- 80.6 nM). Thus, the EIA method combines sensitivity and specificity with the ability to process a large number of specimens to quantify nitrotyrosine produced with in vivo and in vitro sources.
    No preview · Article · Sep 2002 · Nitric Oxide
  • Ken-ichi Hisamatsu · Mayumi Nakajima
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    ABSTRACT: To investigate the effect of pranlukast on leukotriene- induced airway mucosal epithelial dysfunction, samples of human nasal mucosa obtained during surgery for facial trauma were exposed to leukotriene C4 and/or D4 and observed on a TV screen magnified x 2,500. Leukotriene C4- and D4-induced ciliary inhibition and delayed mucosal surface alterations appeared several hours later. Pranlukast prevented both the mucosal epithelial cell dysfunction and the delayed epithelial cell alteration.
    No preview · Article · Nov 2000 · Life Sciences
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    ABSTRACT: Clinical features of acute epiglottitis were studied in 93 patients, 74 males and 19 females ranging from 19 to 78 years of age (mean age: 46.0 years), who had been admitted to our department over the last 10 years. The mean duration of illness was 2.3 days, and the mean period of hospitalization was 7.8 days. Then white blood cell count had increased to 16, 000/mm3 and their CRP to 9.8 mg/dl on average. A smoking habit was noted in 69 of the 93 patients (73.5%) . Thirteen patients (14.0%) had diabetes mellitus as an underlying disease. Forty-five patients (48.4%) had a prior history of therapy at other institutions. Airway control was required in 7 patients (7.5%) . It was suggested that acute epiglottitis develops rapidly, although it improves smoothly after appropriate therapy. It was also suggested that airway obstruction may occur in patients with associated diabetes mellitus or in those who visit a hospital within a couple of days after the onset of symptoms. Patients with acute epiglottitis often visit clinicians specializing in fields other than otolaryngology. Therefore, otolaryngologists should try to inform other clinicians about the clinical features of acute epiglottitis.
    No preview · Article · Jan 1998 · Nihon Kikan Shokudoka Gakkai Kaiho

  • No preview · Article · Jan 1997
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    ABSTRACT: A study was undertaken to evaluate the incidence of alpha-streptococci with inhibitory activity against pathogens, a defense mechanism of the normal flora against bacterial infection, in the nasopharynx and tonsils of 37 children with otitis media with effusion (OME). In the patients with OME, the detection rates of alpha-streptococci with inhibitory activity against Haemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus and group A streptococci in the nasopharynx were significantly lower than those of alpha-streptococci isolated from the tonsils. Furthermore, the detection rates of nasopharyngeal alpha-streptococci with inhibitory activity against H. influenzae and S. pneumoniae in OME patients were lower than those in patients with tonsillitis. In conclusion, low nasopharyngeal levels of alpha-streptococci with inhibitory activity against pathogens appear to render children susceptible to attacks of OME. We suggest that it is important to investigate the relationships between the prevalence of pathogens and the inhibitory activity of alpha-streptococci against them in the nasopharynx.
    No preview · Article · May 1996 · ORL
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    ABSTRACT: To clarify the effects of leukotriene C4 (LTC4) on human ciliated epithelium, ciliary activity of the ethmoid sinus mucosa was measured photoelectrically in tissue culture. At concentrations ranging from 10(-6)M to 10(-9)M, LTC4 showed minimal effects on the ciliated epithelium during the initial 30 minutes of exposure; thereafter, ciliary inhibition was observed in a concentration- and time-dependent manner. Irrigation of the mucosa with culture medium 15 minutes after exposure prevented the LTC4-induced ciliary inhibition. However, irrigation 60 minutes after exposure failed to inhibit 10(-8)M LTC4-induced ciliary dysfunction and mucosal damage. The LTC4-induced ciliary inhibition was blocked in the presence of FPL-55712 and/or Ly-171883, both leukotriene receptor antagonists. L-serine and sodium tetraborate complex (SBC), a gamma-glutamyl transpeptidase (gamma-GTP) inhibitor, also inhibited the LTC4-induced ciliary inhibition. These findings indicate that LTC4 is converted to LTD4 by gamma-GTP during 60 minutes of exposure, and LTC4 itself has minimal direct effects on the ciliated cells.
    No preview · Article · Feb 1996 · Prostaglandins
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    ABSTRACT: The incidence of oral alpha-streptococcus with inhibitory activity against group A streptococcus, as a defense mechanism against bacterial infection in the oral cavity, was investigated in pediatric individuals with tonsillitis. Infection by group A streptococcus appeared to be common in children, because the detection rate of inhibitory alpha-streptococcus in healthy children as well as pediatric patients with tonsillitis was lower than in adults and elderly patients. In particular, the detection rate of these strains was predominantly low in patients with beta-streptococcus. Among pediatric patients scheduled for tonsillectomy, the detection rate of inhibitory alpha-streptococcus was low preoperatively. However, the rate was markedly increased after surgery. The high postoperative detection rate of these strains reflected the decreased incidence of group A streptococcal infection. The results of this investigation of bacterial interference between oral alpha-streptococcus and group A streptococcus suggested that surgical treatment is a more effective approach for improving the oral bacterial flora in children with recurrent tonsillitis.
    No preview · Article · Dec 1995 · International Journal of Pediatric Otorhinolaryngology
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    ABSTRACT: We investigated the distribution of oral alpha-streptococci with inhibitory activity against pathogens, which compose an oral defense mechanism. Detection rate of alpha-streptococci with inhibitory activity against S. pyogenes and S. aureus derived from the tonsil was higher than in other portions, the tongue, cheek, gingiva, or saliva. It has suggested that tonsillar bacterial flora are mainly a defense mechanism. The oral flora in healthy smokers was compared with healthy non-smokers to investigate the influence of tobacco on oral bacterial flora. The results showed that the detection rate of S. aureus in smokers was higher while that of alpha-streptococci with inhibitory activity against S. aureus was lower. However, the detection rate of alpha-streptococci with inhibitory activity against S. pyogenes in smokers was as high as in non-smokers. It is suggested that it was easy for S. aureus to adhere to the oral mucosa in smokers, and was considered to influence the strain which produces beta-lactamase for medical treatment. We investigated the influence of gargling on oral bacterial flora by comparing the amount of bacteria before and after gargling with popidine-iodine gargle and saline solution. It was shown that alpha-streptococci, a main component of normal oral flora were decreased after gargling in both smokers and non-smokers. Furthermore, it was shown that group A Streptococcus was not decreased after gargling, and it was concluded that use of gargle medicinal mouth wash in bacillus carriers should be studied further.
    Preview · Article · Mar 1995 · Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
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    ABSTRACT: Japanese cypress pollinosis has recently attracted attention and its clinical relationship with Japanese cedar pollinosis has been pointed out. To compare the two kinds of pollinosis, we retrospectively examined specific IgE antibodies to both pollen of Japanese cypress and cedar in the sera of 150 patients with nasal allergy using AlaSTAT assay. During the season in which the pollens of these two species are dispersed, the positive rates for Japanese cypress and cedar increased to 51.4 and 75.0%, respectively. The percentage of patients positive for both of cypress and cedar was elevated to 51.4%, corresponding to 68.5% of the total patient group positive for cedar. Almost all the cases positive for cypress had IgE antibodies to cedar, the value of which was considerably higher than that of cases positive only for cedar. Furthermore, increases in titers of specific IgE antibodies to cypress was observed in four of six cases, compared between specific IgE antibodies to cypress in pre- and post-dispersion of cypress pollen. These findings suggest the following possibility: (i) there is cross-antigenicity between the two pollen species, and (ii) patients are immunologically affected by cypress pollen to express higher levels of specific IgE antibodies after pollen dispersion.
    No preview · Article · Feb 1995 · Auris Nasus Larynx
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    ABSTRACT: For a quantitative investigation of eosinophil activation in perennial allergic rhinitis, eosinophil cationic protein (ECP) concentrations were measured by a radioimmunoassay in serum, nasal secretions (ECPWN) and in the supernatant of these nasal secretions (ECPsup) obtained from normal subjects and allergic patients. Levels of ECPWN and ECPsup were higher than that of ECPserum. ECPsup showed a positive correlation with clinical severity, despite the lack of a significant correlation with eosinophilia in nasal smears. ECPWN and ECPserum showed no significant correlation with any of these clinical parameters. There was a weak tendency toward an increase in histamine sensitivity of the nasal mucosa of allergic patients with higher ECPsup although this was not statistically significant. These results suggest accumulation and activation of eosinophils in the allergic nasal mucosa, and also indicate that ECPsup may be a clinical parameter of perennial allergic rhinitis.
    No preview · Article · Feb 1995 · Auris Nasus Larynx
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    ABSTRACT: True malignant mixed tumor, or carcinosarcoma, of the salivary glands is exceedingly rare. We present a case of parotid gland carcinosarcoma occurring in a 57-year-old male patient. His chief complaints were pain and swelling in the left parotid region and difficulty in his mouth open. Despite intensive irradiation, chemotherapy and total parotidectomy, he finally died of local tumor recurrence and distant metastases to the lungs approximately 14 months after the onset of the disease. The surgical specimens and tumor tissue obtained at autopsy revealed typical features of carcinosarcoma consisting of various epithelial and non-epithelial histologic components, including squamous cell carcinoma, undifferentiated carcinoma, chondrosarcoma, spindle cell sarcoma, fibrosarcoma and rhabdomyosarcoma. Immunohistochemical study of the tumor specimens also showed characteristic histochemical diversity and specific features of carcinosarcoma (Keratin, S-100, NSE etc. were positive). Metastatic lesions in both lungs and pulmonary hilar lymph nodes showed the same histological patterns as the original tumor. These findings indicate that this tumor was a true malignant mixed tumor (carcinosarcoma) of parotid gland, as proposed by Batsakis and other investigators.
    No preview · Article · Jan 1995 · Practica Otologica, Supplement
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    ABSTRACT: Shun-ichi Imamura, Kaori Hashimoto, Mayumi Imamura, Izuru Nozawa, Ken-ichi Hisamatsu and Yoshihiko Murakami (Yamanashi Medical University)Peripheral facial palsy caused by secondary malignant tumors of the temporal bone from distant or adjacent primary tumors is thought to be rare. We encountered nine patients with facial palsy resulting from secondary neoplastic invasion to the temporal bone from various primary sites (liver, lung, breast, eyelid, hypopharynx, maxillary sinus, renal pelvis, and leukemia) during a period of approximately 10 years (between 1983 and 1992). The incidence of this type of etiology accounted for 5.2% (9/173) of all patients with facial palsy who were treated at our institution during that period.We also performed histopathologic studies of the temporal bones acquired from five patients who had presented facial palsy of varying degrees before death due to secondary malignant tumors and the correlation between their clinical and histological findings was investigated.The results obtained in the present study were summarized as follows:1) The present study and our comprehensive review of the literature indicated that the most common clinical symptom in patients with secondary malignant tumors of the temporal bone was facial palsy, followed by hearing loss, otalgia, etc.2) It also became apparent that there were three basic routes of tumor invasion from primary tumors to the intratemporal portion of the facial nerve: 1. hematogenous dissemination of neoplastic cells to the bone marrow or pneumatized area of the temporal bone destroying the Fallopian canal,2. direct extension of malignant tumors adjacent to the temporal bone involving the Fallopian canal,3. leptomeningeal metastatic carcinomatosis involving the internal auditory meatus and destroying the facial nerve trunk directly. Clinical otologic features including facial palsy tended to differ depending on the extension route of the malignancy to the temporal bone and facial nerve.3) Although metastatic or secondary temporal bone malignancies are rare and the clinical symptoms usually appear late in the course of disease, otologists should be aware of existence of this disease entity, since otologic symptoms such as that for facial palsy or hearing loss may be the only initial sign of tumor in selected patients.
    No preview · Article · Jan 1995 · Practica Otologica, Supplement
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    ABSTRACT: We examined dizzy patients with orthostatic dysregulation (OD) using the Schellong test, the Cornell Medical Index-Health Questionnaire (CMI) and the Yatabe-Guilford Personality test (Y-G test). Subjects were 134 patients (37 females and 97 females) who were confirmed to have OD based on a questionnaire survey conducted from December 1990 to November 1994.Systolic and diastolic pressures in the supine position in female patients with OD were lower than those in normal subjects. This tendency became more evident with advancing age.Of 17 males with OD, seven (41.2%) were types III or IV on the CMI, and seven (41.2%) were types B or E in the Y-G test which suggested mental and/or emotional "instability". Of 52 females with OD, 26 (50.0%) showed types III or IV on the CMI, and 19 (42.2%) were types B or E on the Y-G test. These results suggest that autonomic imbalance suspected on both the OD questionnaire results and the Schellong test is probably related to the occurrence of vertigo and/or dizziness, and that there is some correlation between OD and emotional and/or psychosomatic instability.
    No preview · Article · Jan 1995 · Equilibrium Research
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    ABSTRACT: Unlike subacute and chronic thyroiditis, acute suppurative thyroiditis with subsequent abscess formation is rarely encountered in clinical practice. The route of infection in recurrent acute suppurative thyroiditis was obscure until Tucker et al first suggested the presence of a congenital fistulous tract extending from the piriform sinus to the side of the neck. The relation between acute suppurative thyroiditis and an internal fistulous tract (piriform sinus fistula) as the route of infection was confirmed by Miyauchi et al. Since then, a number of similar cases have been reported and, at present, piriform sinus fistulae are considered to be the most common route of infection inducing recurrent acute suppurative thyroiditis.Piriform sinus fistula is a persistent congenital sinus tract, which arises from the apex of the piriform sinus and ends in or adjacent to the thyroid lobe, and is characterized by the exclusively leftsided occurrence. The fistulae are assumed to arise from remnants of the 3rd or 4th pharyngeal pouch, or the ultimobranchial body, but its exact origin remains controversial.In this paper, we describe two patients with recurrent acute suppurative thyroiditis and abscess caused by infection through the piriform sinus fistula. Repeated barium meal studies demonstrated a thin fistula in one of the two patients. After fistulectomy, recurrent disease developed in one of the two, indicating incomplete removal of the fistula. This patient underwent revision surgery later. Barium contrast studies and endoscopic examination were useful in making the diagnosis accurately, and surgical exploration of the neck with excision of the entire fistulous tract to the level of the piriform sinus is the treatment of choice.
    No preview · Article · Jan 1995 · Practica Otologica, Supplement
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    ABSTRACT: Non-traumatic cerebrospinal fluid rhinorrhea (CSFR) has a low incidence. However, it should be considered differential diagnosis of running nose because of the risk of recurrent meningitis.In the cases of CSFR with intact olfaction, this site should be conserved during surgery if possible to preserve the patient's quality of life. However, there have been few reports of surgery to cure CSFR via the cribriform plate fistulae. We experienced a patient with unilateral spontaneous CSFR via the cribriform plate, which was successfully cured by a newly developed simple transseptal procedure conserving postoperative olfaction.
    No preview · Article · Jan 1995 · Practica Otologica, Supplement