Seishi ISHINO

National Institute of Animal Health, Tsukuba, Ibaraki, Japan

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Publications (22)5.77 Total impact

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    ABSTRACT: Immunological, enzyme histochemical, and immunohistochemical examinations wereperformed on 19 of 86 lymphomas discovered in a survey of abattoirs in Ibaraki Prefecture. In a fewcases, enzyme histochemistry was helpful in determining lymphoma cell derivation. In 10 follicular centercell lymphomas, tumor cells bearing immunoglobulin (Ig) were identified as B cells. Ig was identified in 2 of 3immunoblastic lymphomas. The remaining immunoblastic lymphoma, however, was of the T-type. It formed rosettewith sheep erythrocytes and expressed leukocyte differentiation cluster 2 (CD2). Three lymphoblasticlymphomas producing similar results were categorized as T-cell neoplasms. The other 3 lymphoblastic lymphomas of the non-T and non-B cell type demonstrated neither T-cell nor B-cell markers. Tumor cells in one T-type lymphoblastic lymphoma were characterized by expressions of both CD4 and CD8 and were thought to derive from a double positive cell in the thymus.
    Preview · Article · Jan 2000
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    ABSTRACT: Histological investigation and classification according to Kiel's human classification were performed on 86 cases of swine lymphoma collected from abattoirs in Ibaraki Prefecture. The fact that the majority (62 cases) were centrocytic or centroblastic lymphomas suggests that B-cell lymphoma is most prevalent among swine lymphoid neoplasms. Although it has been considered rare, follicular proliferation of neoplastic cells was observed in 9 of 32 cases of abdominal lymphoma. Apparently, follicular lymphoma is not very uncommon in the abdominal type. In descending order of frequency, the other cytological types observed were lymphoblastic, immunoblastic, lymphoplasmacytic, lymphocytic, and unclassified.
    Preview · Article · Jan 2000
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    ABSTRACT: Lymphoplasmacytic lymphoma found in a 6-year-old Anglo-Arabian stallion was investigated histologically, immunohistochemically and ultrastructurally. The animal showed a large mediastinal mass and generalized lymph node involvement. The neoplastic cells were in various differentiation stages of small lymphocyte, centrocyte, centroblast, immunoblast and plasma cell. Some neoplastic cells showed positive cytoplasmic reactivity for mu and lambda chains. There were well developed rough endoplasmic reticulum (RER) and Golgi complexes in plasmacytoid cells, and slightly developed RER or a few long strands of RER in medium-sized to large lymphoid cells. These findings suggest that this neoplasm is of B-cell origin.
    No preview · Article · Jul 1993 · Journal of Veterinary Medical Science
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    ABSTRACT: An immunohistochemical analysis of Rhodococcus equi-induced pneumonia in 10 foals was performed by biotin-streptavidin system. The detection of R. equi was more sensitive in immuno-stain using anti-R. equi serum than in Gram's stain. This bacteria also reacted to anti-BCG serum. Lysozyme and alpha 1-antitrypsin were detectable in macrophages. A particularly intense staining was observed in association with intracellular bacteria. Though a degree of reaction for alpha 1-antichymotrypsin was very low in comparison with lysozyme and alpha 1-antitrypsin, it was also demonstrated in macrophages ingesting R. equi. These bacteria were almost intact under an electron microscope. Therefore, the surface components of R. equi may play important roles of protection from intracellular enzymes of macrophages. The cells containing intracytoplasmic IgM, IgG or IgA were a few in number and scattered predominantly around the pneumonic lesion. It is considered that the bactericidal activity by immunoglobulins may be weak in comparison with phagocytosis by macrophages.
    No preview · Article · Jul 1992 · Journal of Veterinary Medical Science
  • H Niizeki · N Hashimoto · S Ishino · K Kadota
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    ABSTRACT: A case of sex cord tumour with annular tubules was found in the right ovary of a 17-month-old Holstein cow. The tumour was characterized by tubules encircling hyaline bodies, which were composed of concentric lamellae of basal lamina. Several irregular tubular structures and a small component of granulosa cell differentiation were seen. Immunohistochemically, the neoplastic cells stained positively for oestradiol or testosterone.
    No preview · Article · Jun 1992 · Journal of Comparative Pathology
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    ABSTRACT: Developing lymphoid tissues of bovine fetuses ranging from 70 to 270 days of fetal age were examined by histological and immunohistochemical procedures. In the peripheral blood, surface membrane immunoglobulin bearing cells (B-lymphocytes) and sheep red blood cell rosette forming cells (T-lymphocytes) had already appeared by 70 days of fetal age. In the lymph nodes intracytoplasmic IgM positive cells appeared at 90 days of fetal age. The cells positive for IgG appeared at 150 days of fetal age and IgA positive cells appeared at 180 days of fetal age. The spleen contained intracytoplasmic immunoglobulin positive cells at almost the same time as those in the lymph nodes. In the ileocecal region, IgM positive cells and IgG positive cells were present at 180 days of fetal age and IgA positive cells were present at 210 days of fetal age. The tonsils contained IgM positive cells and IgG positive cells at 240 days of fetal age. In the thymus, terminal deoxynucleotidyl transferase positive cells appeared at 90 days of fetal age.
    No preview · Article · Nov 1991 · Journal of Veterinary Medical Science
  • S Ishino · K Kadota · T Yoshino · H Yamamoto
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    ABSTRACT: Pathological and immunohistochemical studies were performed on two cases of bovine follicular lymphoma with severe leukaemia and antibodies to bovine leukaemia virus. Histologically, numerous neoplastic follicular structures were observed in the lymph nodes and spleen of both cases and in the tonsils and Peyer's patches of one case. The neoplastic cells infiltrated diffusely into the liver, lungs and heart in both cases and into the tonsils, kidneys, abomasum and bone marrow in one case. Intracytoplasmic immunoglobulin was negative in the neoplastic cells. Follicular dendritic reticulum cells were decreased in number in the neoplastic follicles compared with those in normal lymphoid follicles. Follicular lymphoma is rare in cattle. Histological features of the present cases are distinguishable from the adult form of bovine lymphoma and the calf form of lymphoma.
    No preview · Article · Nov 1990 · Journal of Comparative Pathology
  • K Kadota · S Ishino · N Hashimoto · H Nakajima
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    ABSTRACT: Two cases of swine thymic lymphoma were investigated by electron microscopy and the immunoperoxidase method for terminal deoxynucleotidyl transferase (TdT), and furthermore sheep erythrocyte rosette assay was applied to one case. In case 1, immunoblastoid cells predominated and smaller cells were admixed with them. Many neoplastic cells were TdT-positive and formed rosettes with sheep erythrocytes at 4 degrees C, but about half of them formed rosettes at 37 degrees C. Ultrastructurally the neoplastic cells were characterized by highly irregular nuclei, poorly developed organelles and clustered dense bodies. The neoplasm in case 2 was composed of medium-sized to large cells and the latter predominated. A few of the neoplastic cells stained positively with anti-TdT serum. Ultrastructurally the neoplastic cells were characterized by nuclear convolution, narrow cytoplasmic bands and clustered dense bodies. The morphological features of thymic lymphomas were discussed in comparison with those of other swine lymphomas and lymphomas of other animals. Histological and immunological progression into T-immunoblastoid cells in case 1 was also discussed.
    No preview · Article · Oct 1990 · Zentralblatt für Veterinärmedizin. Reihe A
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    ABSTRACT: Immunoperoxidase technique was applied for pathological study on naturally occurring pneumonic tissues of calves from which Pasteurella haemolytica was isolated. Multifocal necrosis occurred in the lungs of 25 out of 42 calves (59.5%) and P. haemolytica antigen was detected in 22 out of the 25 calves (88.0%). The calves were divided into 3 groups according to the number of P. haemolytica isolated. The positive rate of the bacterial antigen detected by the technique was 66.6% (28/42) on the average, reaching up to 85.7% (18/21) in the group from which the largest number of P. haemolytica was isolated.
    No preview · Article · Jan 1990 · Nippon juigaku zasshi. The Japanese journal of veterinary science
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    ABSTRACT: Fourteen cases of follicular centre cell lymphoma, one case of lymphoplasmacytic lymphoma and one case of lymphoblastic lymphoma (acute lymphoblastic leukaemia) were investigated by electron microscopy. In all cases of follicular centre cell lymphomas, there were nuclear cleavage and slight development of rough endoplasmic reticula, with varide frequencies. Desmosome-like junctions were present in 8 of 14. A lymphoplasmacytic lymphoma revealed a wide range of cell differentiation from small lymphoid cells to immunoglobulin-producing cells containing well-developed rough endoplasmic reticula and among them neoplastic follicular centre cells predominated. The neoplastic cells of lymphoblastic lymphoma lacked characteristic ultrastructures. These lymphomas were distinguishable from T-cell lymphomas with convoluted nuclei. Swine lymphomas were compared with those of other animals and the morphology of lymphomas may be fairly different according to species.
    Preview · Article · Jan 1990
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    ABSTRACT: A case of glioblastoma was found in a 6-month-old Holstein cow. The animal showed ataxia and had a neoplastic nodule occupying a part of the lower cerebellum and the cerebellar peduncle. The neoplastic tissue was composed of pleomorphic cells frequently displaying mitosis and was accompanied by necrosis and haemorrhage. There were many blood vessels and a considerable amount of collagen fibres in part of the neoplastic tissue. Some neoplastic cells were stained positively with anti-glial fibrillary acidic protein (GFAP) serum, and a large number of cells revealed S-100α or S-100β positivity. Neuron-specific enolase (NSE)-positive cells were rarely seen. The histological and immunohistochemical features in the present case are discussed in comparison to those in other primary neoplasms of the nervous system.
    Preview · Article · Jan 1990
  • H Nakajima · S Mabara · S Ishino · K Kadota
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    ABSTRACT: Malignant lymphomas of follicular centre cell (FCC) origin in 14 pigs are described. Seven cases were classified as abdominal type according to the distribution of the neoplastic lesions and the residual cases were of the general type. Most cases were composed of mixed populations of large and medium-sized neoplastic cells and the growing pattern varied from partially follicular to diffuse. The neoplastic cells often showed necrosis, to which macrophages reacted. A part of the neoplastic cells had cytoplasmic immunoglobulin M (CIgM) or cytoplasmic immunoglobulin G (CIgG). The histological features of swine FCC lymphomas are discussed.
    No preview · Article · Nov 1989 · Zentralblatt für Veterinärmedizin. Reihe A
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    ABSTRACT: Different types of lymphoid malignancy were observed in two sheep inoculated with BLV-containing materials. Sheep 1 showed severe leukemic change in the peripheral blood and splenomegaly but lymphosarcoma in the lymph nodes was absent. Sheep 2 had lymphosarcoma in the lymph nodes and various organs. Neoplastic cells had B-cell marker in both cases and a few neoplastic cells contained intracytoplasmic IgM in sheep 2. It was presumed that B-cells might be transformed into neoplastic cells on the way of their differentiation. Some of neoplastic cells might have ability of immunoglobulin-production in sheep 2.
    Preview · Article · Sep 1989 · Nippon juigaku zasshi. The Japanese journal of veterinary science
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    ABSTRACT: Two calves each were inoculated with 1.5 x 10(8) or 5 x 10(9) lymphocytes collected from each one cow which had persistent lymphocytosis (PL) and antibodies to bovine leukemia virus (BLV). A sudden increase in the number of peripheral blood lymphocytes (PBL) was observed 14 and 23 days, respectively, after inoculation and the maximum number reached 29,000 and 52,000/microliters 72 and 57 days after inoculation. Although the degree of PL decreased gradually in these cattle, it continued until 14 and 44 months after inoculation when one animal was sacrificed and the other died of lymphosarcoma. The PL was passaged in cattle by inoculation of a large number of PBL obtained from cattle at the stage of PL (PLL). The degree of PL was severer in cattle inoculated with a larger number of PLL. PL was not caused by inoculation of PBL obtained from either BLV-infected non-PL cattle or cattle free of BLV. The PL was also caused by inoculation of PLL into BLV-infected non-PL cattle. On the other hand, it was not observed after inoculation of a large amount of cell-free virus obtained from short-term cultures of PLL. Antibodies to BLV developed earlier and to higher levels in cattle inoculated with PLL than in those inoculated with cell-free virus. These facts show that infection with BLV was established more effectively by PLL than by cell-free virus, the infection may occur by lymphocyte to lymphocyte interaction and the actual number of infected BLV may have an important role in development of PL.
    No preview · Article · Mar 1989 · Nippon juigaku zasshi. The Japanese journal of veterinary science
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    ABSTRACT: Properties of peripheral blood lymphocytes (PBL) appearing during persistent lymphocytosis (PL) of cattle that was induced by inoculation of a large number of bovine leukemia virus (BLV)-infected lymphocytes were studied. The PBL possessed surface immunoglobulin at a high ratio and had high syncytium-forming activity. The PBL could not be distinguished from normal PBL cytogenetically. These PBL were shown to be polyclonal cells integrating BLV-proviral DNA randomly into their cellular DNA. These properties were similar to those of PBL appearing at the stage of PL of cattle naturally infected with BLV. The BLV-proviral DNA content of PBL and transcription of viral genes increased in parallel with development of PL and decreased along with a decline of PL indicating the possibility that gene expression and activation of BLV play an important role in induction and maintenance of PL.
    No preview · Article · Mar 1989 · Nippon juigaku zasshi. The Japanese journal of veterinary science
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    ABSTRACT: A Ziehl-Neelsen (ZN) stain for acid-fast bacteria and an immunoenzyme technique using anti-BCG serum were applied to paraffin sections of acid-fast bacterial infections in pigs, cattle and turkey. As for swine mycobacteriosis, a few bacilli were found in some lesions in 3 of 5 cases by ZN stain, while a small amount of BCG-positive material was seen in all cases by immunoenzyme method. In lesions of bovine and turkey tuberculosis, a moderate number of bacilli were detected by ZN stain and reactive products of immunostaining were more frequently observed. In bovine paratuberculosis, there were many ZN-positive bacilli and much more reactive products of immuno-staining. Among non-acid-fast bacteria, Rhodococcus equi was stained with anti-BCG serum, but was gram-positive. The immunoenzvme method using anti-BCG serum may be more useful in detecting acid-fast bacteria than ZN stain.
    Preview · Article · Jan 1989
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    ABSTRACT: Small laboratory animals were experimentally inoculated with bovine leukemia virus (BLV). Antibodies against BLV were detected in rabbits and guinea pigs. In the rabbits inoculated with leukocytes from a BLV-infected cow, the antibody titers against BLV glycoprotein (gp) increased within 2 months after inoculation and persisted for more than 7 months in the range between 2 and 128. Infective BLV was not detected in peripheral blood leukocytes from these animals by the syncytium induction assay. However, sheep and rabbits inoculated with leukocytes or blood from BLV-antibody positive rabbits, developed gp antibodies against BLV. These results show that rabbits are susceptible to BLV but not as sensitive as cattle and sheep. However, the rabbit would be applicable as an experimental animal for the study of enzootic bovine leukosis.
    Full-text · Article · Jan 1989 · Nippon juigaku zasshi. The Japanese journal of veterinary science
  • S Ishino · K Kadota · M Nakagawa · T Yoshino

    No preview · Article · Oct 1988 · Zentralblatt für Veterinärmedizin. Reihe A
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    ABSTRACT: The avidin-biotin-complex method was applied to the liver of 17 chickens experimentally infected with avian lymphoid leukosis (LL) for the investigation of cytoplasmic immunoglobulins (CIg). The liver having a few normal lymphocytes or plasma cells was used for this study. In the livers, the neoplastic cells showed a nodular growth pattern. A few CIgM-positive cells were observed in 3 cases. Both CIgM and CIgG were present in the cells of 12 cases. Thus not only these 2 classes but also CIgA was seen in the cells of 2 cases. Although most of the positive cells were large in size, smaller cells occasionally revealed positive reactions. Our study suggests that the heavy chain class switch may occur in the neoplastic cells of LL and that LL is closely related to lymphatic follicles
    Preview · Article · Jan 1988
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    Seishi ISHINO · Muneo NAKAZAWA · Izumi MATSUDA
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    ABSTRACT: The lungs of 32 guinea-pigs inoculated intratracheally with Rhodococcus equi were examined histopathologically in order to study the pathogenesis of R. equi-pneumonia. The most characteristic lesion was suppurative bronchopneumonia, which developed as follows: Congestion, serous exudation and mild infiltration of macrophages carrying Gram-positive bacteria and multinucleate giant cells 1 day postinoculation (pi), infiltration of numerous neutrophils and macrophages in the alveoli 2-5 days pi, focal accumulation of necrotic cells and infiltration of mononuclear cells phagocytizing degenerative neutrophils with a few multinucleate giant cells 6-10 days pi, small accumulation of mononuclear cells surrounded by normal alveolar tissues 12-16 days pi. The lung lesions in R. equi-infected guinea-pigs healed more easily as compared with those in the foals.
    Preview · Article · Jul 1987 · Nippon juigaku zasshi. The Japanese journal of veterinary science