Jian-hua Qian

Zhejiang University, Hangzhou, Zhejiang Sheng, China

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Publications (4)0 Total impact

  • Jian-yun Xu · Feng Ye · Wei-guo Lü · Die Hong · Jian-hua Qian · Xing Xie
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    ABSTRACT: To compare genomic expression differences between androgenic complete hydatidiform mole (AnCHM) and normal first trimester villi with similar gestation weeks, and search for potential adjuvant diagnostic molecular markers. Short tandem repeat (STR) detection was used to identify AnCHM, human oligonucleotide array U133 Plus 2.0 was used to measure genomic expression differences between AnCHM and normal villi, and quantitative fluorescent RT-PCR was used to verify array of several genes. Nine of 11 histologically diagnosed complete hydatidiform moles were found to be AnCHM by means of STR, and the other 2 were biparental complete hydatidiform mole (BiCHM). Compared with villi, oligonucleotide array showed 279 genes (0.72%, 279/38 500) were over expressed and 1710 genes (4.44%, 1710/38,500) under expressed in AnCHM. Bioinformatics analysis found that differentially expressed genes were involved in multiple biological processes and pathways. Changes of imprinting genes, growth hormone genes and chorionic somatomammotropin hormone genes were especially remarkable. Pathogenesis of AnCHM is a complex process involving multiple genes and pathways. Altered expression of imprint genes may play important roles in the process.
    No preview · Article · Sep 2007 · Zhonghua fu chan ke za zhi
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    ABSTRACT: Through detection of 9 loci of polymorphisms of microsatellite to investigate the parental origin of complete hydatidiform mole. Using the technology and method of multipolymerase chain reaction and electrophoresis in denatured polyacrylamide gel and silver stain detection, we carried out DNA analysis on 50 cases of complete hydatidiform mole diagnosed by histopathology and 50 copies of the couples' peripheral blood. There are 7 cases of biparental complete hydatidiform mole (14%); and 43 cases of androgenetic complete hydatidiform mole (86%) among 50 cases of complete hydatidiform mole. Detection of polymorphisms of microsatellite is a technology that can be used to identify the parental origin of complete hydatidiform mole. It is simple, quick, reliable and highly efficient.
    No preview · Article · Aug 2007 · Zhonghua fu chan ke za zhi
  • Ya-xia Chen · Yuan-ming Shen · Jian-hua Qian · Xing Xie
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    ABSTRACT: To evaluate the effects of primary chemotherapy with single-agent methotrexate (MTX) on low-risk gestational trophoblastic neoplasia (GTN) and the influencing factors thereof. Sixty-one GTN patients with the score of < or = 6 according to the new International Federation of Gynecology and Obstetrics (FIGO) scoring system (2000) were divided into 2 groups: 51 patients were treated with single MTX 0.4 mg/kg daily for 5 days (MTX 5 d group), and 10 patients were treated with MTX on the days 1, 3, 5, and 7, and with folinic acid (FA) 0.1 mg/kg on the days 2, 4, 6, and 8 (MTX + FA group), both group with an interval of treatment course of 2 weeks. The serum level of human chorionic gonadotropin (hCG) was detected every week. If a plateau or increase of serum hCG appeared between 2 examination results, meaning tolerance to MTX, the patients concerned had to undergo different regimens of salvage chemotherapy, all with MTX as one of their components. Univariate and multivariate methods were used to analyze the relationships of different factors to the outcomes of chemotherapy. Thirty-five of the 51 patients of the MTX 5d group (68.6%) achieved complete primary remission, 3 of the 10 patients of the MTX + FA group achieved complete primary remission, and all patients achieved complete remission after salvage chemotherapy. Univariate analysis showed that the mean pretreatment serum level of hCG, duration between antecedent pregnancy and start of treatment, size of tumor, FIGO score, specific regimen of MTX were significantly associated with outcome of chemotherapy (P = 0.004, 0.022, 0.017, 0.005, 0.021 respectively). Logistic regression analysis showed that only three independent factors predictive for the outcome of chemotherapy: MTX regimen (OR = 2.476), FIGO score (OR = 1.431), and pretreatment hCG titer (OR = 1.001). Primary chemotherapy with single MTX regimen may still be one of the options for patients with low-risk GTN according to the new FIGO scoring system, though the rate of complete primary remission appears to be lower. All patients with low-risk GTN achieve complete remission after salvage chemotherapy. MTX regimen, FIGO score, and pretreatment hCG are independent risk factors of outcome of single MTX chemotherapy.
    No preview · Article · Aug 2005 · Zhonghua yi xue za zhi
  • Jian-hua Qian · Da-fen Ye · Xing Xie
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    ABSTRACT: To evaluate clinical-pathological features, diagnosis and therapy of gestational trophoblastic tumor (GTT) misdiagnosed as ectopic pregnancy. From 1999 to 2003, a total of 13 patients with GTT misdiagnosed as ectopic pregnancy were retrospectively analyzed. The main symptoms were amenorrhea, abdominal pain, irregular vaginal bleeding. Serum beta-human chorionic gonadotrop in (hCG) was measured in 10 patients. Eight had hCG values above 10,000 IU/L; 3 had hCG values above 50,000 IU/L. The lesions of GTT misdiagnosed as ectopic pregnancy were fallopian tube, horn of uterus, peritoneal cavity, greater omentum, recto-uterine pouch. According to standards of the International Federation of Gynecology and Obstetrics (FIGO) the 13 patients were categorized as 6 of stage I, 2 of stage II, 3 of stage III and 5 of stage IV. Histologically they included 10 cases of choriocarcinoma and 3 of invasise mole. All patients were treated by complete surgical resection combined with subsequent adjuvant chemotherapy. Misdiagnosis leads to delay in therapy with resultant increased morbidity of GTT. Analysis on serial hCG is helpful to differential diagnosis between ectopic pregnancy and GTT.
    No preview · Article · Mar 2005 · Zhonghua fu chan ke za zhi