Eun-Kyoung Pang

Ewha Womans University, Sŏul, Seoul, South Korea

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Publications (21)20.03 Total impact

  • Gyu-Un Jung · Seong-Jin Hong · Ji-Youn Hong · Eun-Kyoung Pang
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    ABSTRACT: The aim of this study was to evaluate the effect of human freeze-dried bone block (FDBB) and deproteinized bovine bone with collagen (DBBC) on bone formation when applied as an onlay graft in rat calvariums. Thirty male Sprague-Dawley rats received collagen sponge (control), FDBB, or DBBC onlay grafts trimmed into 8-mm disks measuring 4-mm height. Each graft was secured onto the calvarium surface using horizontal mattress sutures. Rats in each group were killed at 2 (n=5) or 8 (n=5) weeks postoperatively for histologic and histomorphometric analysis. The total augmented area (mm2), new bone area (mm2), and bone density (%) were measured. The FDBB and DBBC groups showed significantly more new bone formation and bone density than the control group at 2 and 8 weeks. The increased new bone area was significantly greater in the FDBB group than in the DBBC group (p<0.05). The total augmented area was significantly higher in the FDBB and DBBC groups at 2 and 8 weeks than in the control group (p<0.05), and at 8 weeks, the area was significantly decreased in the DBBC group compared to that in the FDBB group and the area at 2 weeks (p<0.05). Within the limitations of the present study, we concluded that onlay FDBB and DBBC grafts caused new bone formation through an osteoconductive mechanism. In addition, compared to FDBB, DBBC had less capacity to form new bone and maintain the space.
    No preview · Article · Feb 2016 · Tissue Engineering and Regenerative Medicine
  • Gyu-Un Jung · Sun-Kyoung Lee · Suk Ji · Eun-Kyoung Pang
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    ABSTRACT: Periodontal ligament cells (PDLCs) play an important role in the regeneration of periodontium. The healing potential of PDLCs may be due to the high concentration of growth factors in platelet-rich plasma (PRP). In this study, the effects of PRP on the proliferation and activation of PDLCs and growth factor release from PDLCs were investigated. PDLCs were isolated from third molars or premolars of healthy patients. Whole blood was obtained from healthy volunteers for the preparation of activated PRP. The platelet concentration in PRP was measured and the amount of platelet-derived growth factor (PDGF)-AB, PDGF-BB, transforming growth factor-β1 (TGF-β1), and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assay. The effects of activated PRP on PDLC proliferation, attachment, alkaline phosphatase (ALP) activity, and growth factor release were investigated. Platelet concentration was increased 5.41-fold in PRP compared to whole blood. PDGF-AB, PDGF-BB, TGF-β1, and VEGF were detected in PRP at concentrations of 273.38 ng/mL, 47.0 ng/mL, 168.42 ng/mL, and 510.56 pg/mL, respectively. PDLCs cultured with ≥10% PRP showed significantly increased cell proliferation and ALP activity compared to the control (p<0.05). PDLCs cultured with 10% PRP also presented higher cell attachment and increased release of TGF-β1 and VEGF compared to the control (p<0.05). PRP can deliver high concentrations of growth factors to a defect site to increase the proliferation, attachment, and ALP activity of PDLCs. These results suggest that PRP might effectively contribute to periodontal regeneration.
    No preview · Article · Dec 2015 · Tissue Engineering and Regenerative Medicine
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    ABSTRACT: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed. All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks. Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.
    Preview · Article · Jul 2015
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    Ju-Hee Hong · Gyu-Un Jung · Sang-Wan Shin · Eun-Kyoung Pang
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    ABSTRACT: The purpose of this prospective study was to compare the satisfaction levels of the patients worn implant supported mandibular overdentures and conventional complete dentures.
    Preview · Article · Jan 2015 · The Journal of Korean Academy of Prosthodontics
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    Gyu-Un Jung · Jin-Woo Kim · Sun-Jong Kim · Eun-Kyoung Pang
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    ABSTRACT: The purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (PT) as an adjunct to scaling and root planing (SRP) in patients with chronic periodontitis. The study was conducted from December 2013 to May 2014 at Ewha Womans University Mokdong Hospital, Seoul, Korea. Forty-one patients with mild to moderate chronic periodontitis were randomly divided into two therapeutic groups in a 1:1 ratio: SRP+PT and SRP (control) groups. All participants underwent full-mouth SRP. PT was performed thrice a day for a month by using electric toothbrushes with embedded light-emitting diodes. Plaque index, gingival index, probing pocket depth (PPD), clinical attachment level (CAL), and bleeding on probing were assessed before (baseline) and four weeks after (follow-up) the treatment. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, Eikenella corrodens, Streptococcus mutans, and Streptococcus sobrinus levels were detected by a real-time polymerase chain reaction at the same points in time. The clinical parameters improved in both the groups. At the follow-up assessment, PPD was significantly decreased in the SRP+PT group (P=0.00). Further, PPD and CAL showed significantly greater changes in the SRP+PT group than in the SRP group (PPD, P=0.03; CAL, P=0.04). P. gingivalis and T. forsythia levels decreased in this group, but no significant intergroup differences were noted. Adjunctive PT seems to have clinical benefits, but evidence of its antimicrobial effects is not sufficient. Long-term studies are necessary to develop the most effective PT protocol and compare the effectiveness of PT with and without exogenous photosensitizers.
    Full-text · Article · Dec 2014 · Journal of periodontal & implant science
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    Gyu-Un Jung · Eun-Kyoung Pang · Chang-Joo Park
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    ABSTRACT: Purpose: In the anterior maxilla, hard and soft tissue augmentations are sometimes required to meet esthetic and functional demands. In such cases, primary soft tissue closure after bone grafting procedures is indispensable for a successful outcome. This report describes a simple method for soft tissue coverage of a guided bone regeneration (GBR) site using the double-rotated palatal subepithelial connective tissue graft (RPSCTG) technique for a maxillary anterior defect. Methods: We present a 60-year-old man with a defect in the anterior maxilla requiring hard and soft tissue augmentations. The bone graft materials were filled above the alveolar defect and a titanium-reinforced nonresorbable membrane was placed to cover the graft materials. We used the RPSCTG technique to achieve primary soft tissue closure over the graft materials and the barrier membrane. Additional soft tissue augmentation using a contralateral RPSCTG and membrane removal were simultaneously performed 7 weeks after the stage 1 surgery to establish more abundant soft tissue architecture. Results: Flap necrosis occurred after the stage 1 surgery. Signs of infection or suppuration were not observed in the donor or recipient sites after the stage 2 surgery. These procedures enhanced the alveolar ridge volume, increased the amount of keratinized tissue, and improved the esthetic profile for restorative treatment. Conclusions: The use of RPSCTG could assist the soft tissue closure of the GBR sites because it provides sufficient soft tissue thickness, an ample vascular supply, protection of anatomical structures, and patient comfort. The treatment outcome was acceptable, despite membrane exposure, and the RPSCTG allowed for vitalization and harmonization with the recipient tissue.
    Full-text · Article · Jun 2014 · Journal of periodontal & implant science
  • Hyejin Chung · Ji-Youn Hong · Gyu-Un Jung · Eun-Kyoung Pang
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    ABSTRACT: The aim of this study was to investigate the effect of an onlay graft of human freeze dried corticocancellous bone block (FDBB) on bone formation and the added effects of collagen membrane (CM) in rat calvarium. Thirty male Sprague-Dawley rats were treated with either collagen sponge (CS), FDBB or FDBB with CM. FDBBs were placed on the calvarium surface with the CM covered or not. Rats were sacrificed at 2 and 8 weeks after surgery for histologic and histomorphometric analysis. At each period, total augmented area (TA; mm2), new bone area (NB; mm2), and bone density (BD; %) were measured. In the FDBB and the FDBB/CM group, new bone formation began at the lateral and inferior margins of the grafted block and projected into the central region of the recipient-graft interface. The cancellous portion of the graft underwent increased resorption with time. FDBB showed a significant decrease in the TA between 2 and 8 weeks (p
    No preview · Article · Apr 2014 · Tissue Engineering and Regenerative Medicine
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    Ah-Reum Lee · Chung-Hoon Chung · Gyu-Un Jung · Eun-Kyoung Pang
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    ABSTRACT: This study is designed to investigate the various impacts of different types of scaler tips such as cooper alloy base tip and the others on the surface roughness of teeth and implant by the method which is currently in clinical use.
    Preview · Article · Jan 2014 · The Journal of Korean Academy of Prosthodontics
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    ABSTRACT: Objectives The objective of this study was to elucidate the socket healing process and biodegradation of incorporating synthetic bone fillers followed by grafting of the fresh extraction socket. Materials and methodsThird premolars in four quadrants of eight beagle dogs were extracted and randomly treated with either one of hydroxyapatite (HA), biphasic calcium phosphate (BCP), -tricalcium phosphate (-TCP), or no graft (C). Histologic observations and histomorphometric analysis at three zones (apical, middle, and coronal) of the socket were performed. Socket area (S) and the proportions of newly formed bone (%NB), residual biomaterials (%RB), and fibrovascular connective tissue (%FCT) at 2, 4, and 8weeks were measured. The numbers of osteoclast-like multinucleated cells (No.OC) were also determined at the three zones. Results%NB was significantly higher in control group compared with the grafted groups at all healing periods. %NB of HA and BCP increased with time, whereas %RB showed different patterns that decreased in BCP, unlike the minimal change observed in HA. %NB of -TCP showed smallest portion compared with other grafted groups at 2 and 4weeks, however, significantly increased at 8weeks. %RB of -TCP was less than HA and BCP at all healing periods. Numbers of multinucleated cells were greater in BCP and -TCP, followed by HA and smallest in control group. Conclusions Within the limit of this study, bone formation of the extraction socket was delayed in the sockets grafted with synthetic bone fillers and showed different healing process according to the biodegradation patterns.
    No preview · Article · Sep 2012 · Clinical Oral Implants Research
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    Deug-Han Kim · Ji-Youn Hong · Eun-Kyoung Pang
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    ABSTRACT: This study was aimed to evaluate the effect of the Freeze Dried Bone Allograft and Demineralized Bone Matrix on osseous regeneration in the rat calvarial defects.
    Preview · Article · Jan 2009
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    ABSTRACT: The purpose of the present study was to analyze the implant stability quotient(ISQ) values for Korean non-submerged type implant and determine the factors that affect implant stability.
    Preview · Article · Jan 2009
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    Hye-Ran Jeon · Eun-Kyoung Pang · Ah-Ran Pae · Myung-Rae Kim · Na-Ra Kang
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    ABSTRACT: The aim of this study was to determine the survival rates of implants placed in grafted maxillary sinuses and compare the results obtained with graft materials, implant surfaces and timing of implant placement.
    Full-text · Article · Jan 2008
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    Sun-Kyoung Lee · Suk Ji · Eun-Kyoung Pang
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    ABSTRACT: Studies reported controversial results about prognosis of root resection with 0%-38% failure rate. The purpose of this study is to consider clinical efficacy of root resection.
    Preview · Article · Jan 2008
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    Deug-Han Kim · Suk Ji · Eun-Kyoung Pang
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    ABSTRACT: During guided bone regeneration procedures for the augmentation of deficient alveolar ridge, primary closure of flap is necessary. For primary flap closure, flap is repositioned coronally and the zone of attached keratinized mucosa may decreased. The need for attached keratinized mucosa around dental implants is still controversial, but sufficient peri-implant attached keratinized mucosa would be beneficial for functional and esthetic aspects. This case report presents three cases that demonstrated free gingival graft for increasing the zone of peri-implant attached keratinized mucosa which was decreased after guided bone regeneration.
    Preview · Article · Jan 2008
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    Ju-Mi Park · Hye-Ran Jeon · Eun-Kyoung Pang · Myung-Rae Kim · Na-Ra Kang
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    ABSTRACT: The aim of this study was to evaluate adhesion and gene expression of the MC3T3-E1 cells cultured on machined titanium surface (MS) and anodized titanium surface (AS) using MTT test, Scanning electron micrograph and cDNA microarray.
    Full-text · Article · Jan 2008
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    ABSTRACT: The edentulous posterior maxilla generally provides a limited amount of bone height because of atrophy of the ridge and pneumatization of the maxillary sinus. Maxillary sinus augmentation is one of the surgical techniques for reconstruction of the severely resorbed posterior maxilla.
    Preview · Article · Jun 2007
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    ABSTRACT: Beta tricalcium phosphate (beta-TCP) has been developed as one of the carriers of recombinant human bone morphogenetic protein (rhBMP). However, it is not known whether the particle size of beta-TCP is related to its resorption rate and the degree of bone formation. The purpose of this study was to evaluate the effect of using beta-TCP with different particle sizes on the ability of rhBMP-4 to enhance bone formation in the rat calvarial defect model. Calvarial, 8-mm-diameter, critical-size defects were created in 100 male Sprague-Dawley rats. Five groups of 20 animals each received either rhBMP-4 (2.5 microg) using beta-TCP with a particle size of 50 to 150 microm, rhBMP-4 (2.5 microg) using beta-TCP with a particle size of 150 to 500 microm, a beta-TCP control with a particle size of 50 to 150 microm, a beta-TCP control with a particle size of 150 to 500 microm, or a sham-surgery control, respectively, and were evaluated by measuring their histologic and histometric parameters following a 2- and 8-week healing interval. There were no significant differences in the defect closure, new bone area, or augmented area between either the two rhBMP-4/beta-TCP groups or between the two beta-TCP control groups at 2 and 8 weeks. rhBMP-4 combined with either small- or large-particle beta-TCP had a significant effect on the induction of bone formation compared to either a small- or large-particle beta-TCP control or a sham-surgery control. Within the parameters of this study, varying the particle size of beta-TCP did not seem to have a significant effect on bone formation.
    No preview · Article · Jun 2006 · Journal of Periodontology
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    ABSTRACT: The purpose of this study was to evaluate the effect of chitosan on human periodontal ligament fibroblasts (hPDLF) in vitro and on bone formation in rat calvarial defects in vivo. Fibroblast populations were obtained from individuals with a healthy periodontium and cultured in alpha minimum essential medium (MEM) for the control group. For the experimental groups, cells were cultured in alpha-MEM containing chitosan at concentrations of 0.01, 0.1, 1, or 2 mg/ml. The 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR) and the assay of alkaline phosphatase (ALPase) activity were performed. Eight mm calvarial critical-sized defects were created in 30 male Sprague-Dawley rats. The animals were divided into three groups of 10 animals each. The defects were treated with either chitosan/absorbable collagen sponge (ACS) or ACS alone in the experimental groups or were left untreated (surgical controls). The animals were sacrificed at 2 or 8 weeks post-surgery and the treatment outcomes were evaluated using histological and histomorphometric parameters. The chitosan-induced proliferative responses of the hPDLF reached a plateau at a concentration of 0.1 mg/ml (P <0.05). When the hPDLF were stimulated with 0.1 mg/ml chitosan, both the mRNA expression of type I collagen and the ALP activity were significantly up-regulated (P <0.05). The surgical implantation of chitosan/ACS enhanced the new bone formation at 8 weeks post-surgery and the amount of new bone formation of the chitosan/ACS group was significantly greater than that of both the ACS alone group and the surgical control group (P <0.01). The new bone area and defect closure in the chitosan/ACS group were significantly greater than those in the ACS control and sham surgery control groups at 8 weeks (P <0.01). However, the chitosan/ ACS group exhibited significantly less bone density than both the ACS control and the sham surgery control group at 8 weeks (P <0.01). Chitosan (0.1 mg/ml) enhanced the type I collagen synthesis and facilitated the differentiation into osteogenic cells. Chitosan reconstituted with ACS has a significant potential to accelerate the regeneration of bone in rat calvarial critical size defects.
    No preview · Article · Sep 2005 · Journal of Periodontology
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    ABSTRACT: Bone morphogenetic proteins (BMPs) are being evaluated for periodontal and bone regenerative therapy. The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein-4 (rhBMP-4) dose on local bone formation in a rat calvaria defect model. Calvarial, 8 mm diameter, critical-size osteotomy defects were created in 140 male Sprague-Dawley rats. Seven groups of 20 animals each received either 1) rhBMP-4 (2.5 microg) in an absorbable collagen sponge (ACS) carrier, 2) rhBMP-4 (5 microg)/ACS, 3) rhBMP-4 (2.5 microg) in a beta-tricalcium phosphate (beta-TCP) carrier, 4) rhBMP-4 (5 microg)/beta-TCP, 5) ACS or 6) beta-TCP carrier controls, or 7) a sham-surgery control, and were evaluated by histologic and histometric parameters following a 2- or 8-week healing interval (10 animals/group/healing interval). Surgical implantation of rhBMP-4/ACS and rhBMP-4/beta-TCP resulted in enhanced local bone formation at both 2 and 8 weeks. Within the dose range examined, rhBMP-4 did not exhibit an appreciable dose-dependent response. Defect closure was not significantly different between the rhBMP-4/ACS and rhBMP-4/beta-TCP groups. New bone area of the rhBMP-4/ beta-TCP group was significantly greater than that of the rhBMP-4/ ACS group; however, bone density in the rhBMP-4/ACS group was significantly greater than that in the rhBMP-4/beta-TCP group at 8 weeks (P < 0.05). rhBMP-4 combined with ACS or beta-TCP has a significant potential to induce bone formation in the rat calvaria defect model. Within the selected rhBMP-4 dose range and observation interval, there appeared to be no meaningful differences in bone formation.
    No preview · Article · Nov 2004 · Journal of Periodontology
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    ABSTRACT: Alveolar bone resorption is a characteristic feature of periodontal diseases and involves the removal of both the mineral and organic constituents of the bone matrix, which is caused by either multinucleated osteoclast cells or matrix metalloproteinases (MMPs). The gram-negative bacterium, Porphyromonas gingivalis has been reported to stimulate the activity and expression of several groups of MMPs, whereas (-)-epigallocatechin gallate (EGCG), the main constituent of green tea polyphenols, has been reported to have inhibitory effects on the activity and expression of MMPs. In the present study, we investigated the effects of the green tea polyphenol, EGCG, on the gene expression of osteoblast-derived MMP-2, -9 and -13, stimulated by P. gingivalis, and on the formation of osteoclasts. The effect of EGCG on the gene expression of MMPs was examined by treating mouse calvarial primary osteoblastic cells with EGCG (20 microM) in the presence of sonicated P. gingivalis extracts. The transcription levels of MMP-2, -9 and -13 were assessed by reverse transcription-polymerase chain reaction (RT-PCR). The effect of EGCG on osteoclast formation was confirmed by tartrate-resistant acid phosphatase (TRAP) staining in a co-culture system of mouse bone marrow cells and calvarial primary osteoblastic cells. Treatment with the sonicated P. gingivalis extracts stimulated the expression of MMP-9 mRNA and this effect was significantly reduced by EGCG, whereas the transcription levels of MMP-2 and MMP-13 were not affected by either the sonicated P. gingivalis extracts or EGCG. In addition, EGCG significantly inhibited osteoclast formation in the co-culture system at a concentration of 20 microM. These findings suggest that EGCG may prevent the alveolar bone resorption that occurs in periodontal diseases by inhibiting the expression of MMP-9 in osteoblasts and the formation of osteoclasts.
    No preview · Article · Nov 2004 · Journal of Periodontal Research

Publication Stats

198 Citations
20.03 Total Impact Points

Institutions

  • 2009-2014
    • Ewha Womans University
      • School of Medicine
      Sŏul, Seoul, South Korea
  • 1999-2006
    • Yonsei University
      • College of Dentistry
      Sŏul, Seoul, South Korea