[Show abstract][Hide abstract] ABSTRACT: Este estudo investigou a ocorrência de anticorpos anti-Neospora caninum e anti-Toxoplasma gondii por ensaio imunoenzimático indireto (ELISA) e reação de imunofluorescência indireta (RIFI), assim como os fatores de risco associados à toxoplasmose e à neosporose em 204 cães provenientes de áreas urbana e rural do município de Araguaína, Estado de Tocantins, Brasil. Cento e trinta amostras (63,7%) foram positivas para T. gondii, destas 57,1% e 70,7% oriundas de áreas urbanas e rurais, respectivamente. Considerando-se o teste RIFI, a frequência de soropositividade para T. gondii foi de 57,4% com distribuição de 62,6% e 52,4% entre áreas rurais e urbanas, respectivamente. Fatores associados à toxoplasmose canina foram raça e idade, com soropositividade maior para animais mais velhos (p<0,05). Em relação à N. caninum, 88 (43,1%) amostras foram positivas, segundo ELISA, sendo distribuídas em 42,9% para área urbana e 43,3% para área rural. Por meio da RIFI, anticorpos anti-N. caninum foram detectados em 62 (30,4%) cães, sendo distribuídos em 31,3% e 29,5% entre áreas rurais e urbanas, respectivamente. Os fatores associados à ocorrência de neosporose, pela RIFI, foram idade e raça (p<0,05). Este estudo representa a primeira detecção de anticorpos IgG para toxoplasmose e neosporose canina no Estado de Tocantins e evidencia a importância de cães na cadeia epidemiológica dessas doenças.
Preview · Article · Oct 2015 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: Pr1 is a subtilisin-like protease produced by Metarhizium spp. entomopathogenic fungi, and it is recognized as heavily involved in the initial steps of the fungal invasion of arthropod-host cuticles. In the current study, correlation was sought between mortality of tick larvae and conidial Pr1 levels of one Metarhizium anisopliae senso latu (s.l.) isolate (CG 148). Conidia with different levels of pr1 gene expression and enzymatic activity were obtained by producing them on either artificial medium (to yield low Pr1 activity) or on Rhipicephalus microplus cadavers (to yield high Pr1 activity). Conidial proteolytic activity was assessed using N-suc-ala-ala-pro-phe-ρNA as the chromogenic substrate, and pr1 expression was profiled by qPCR using three genes (gpd, try, and tef) as reference genes. Pr1 enzymatic (proteolytic) activity on conidia obtained from tick cadavers was 36 U mg(-1) in comparison to 4 U mg(-1) on conidia from PDA medium. Also, pr1 gene expression level was ten times higher in conidia from tick cadavers compared to PDA medium. Bioassays of M. anisopliae s.l. CG 148 spores with elevated Pr1 proteolytic activity and gene expression levels did not demonstrate increased virulence (= significant change percent mortality of tick larvae). The minimal levels of Pr1 on conidia produced on artificial medium was adequate to afford high levels of virulence, and the elevated amounts of the enzyme on tick-cadaver-produced conidia did not induce elevated larval mortality. As long as some Pr1 activity was present, fungal virulence of isolate CG 148 against tick larvae was not elevated by increased levels of conidial Pr1.
Full-text · Article · Mar 2015 · Parasitology Research
[Show abstract][Hide abstract] ABSTRACT: This study aimed to assess and evaluate the effects of Theileria equi infection on embryonic recovery, gestation and early embryonic loss. Thirteen Mangalarga Marchador Theileria equi positive donors (diagnosed through nested-PCR) and 40 embryos receptors were used. Donors were submitted to two embryo collections in two consecutive estrous cycles (GId); after, the same mares were treated with imidocarb dipropionate (1.2mg/kg IM.) in order to collect more embryos in two more estrous cycles (GIId). Receptors were divided into two groups (control and with treated) with 20 animals each, where one group was the control (GIr) and the other one (GIIr) treated with 1.2mg/kg IM of imidocarb dipropionate assessing the gestation rate at 15, 30, 45 and 60 days. After 52 embryo collections, the embryonic recovery rates were 53.84% (14/26) and 65.38% (17/26) (p > 0.05) for GId and GIId, respectively. The gestation rate was 70% (14/20) (p > 0.05) at 15, 30, 45 and 60 days in group GIr and for GIIr was 85% (17/20) (p > 0.05) at 15 days, 80% (16/20) (p > 0.05) at 30, 45 and 60 days. The treatment with imidocarb dipropionate did not cause significant improvement in the reproductive efficiency at an ET program.
[Show abstract][Hide abstract] ABSTRACT: From the sequencing of the entire genome of Anaplasma marginale isolates, genes encoding
membrane proteins of this hemoparasite have been identified, as well as novel immunogens as potential vaccine candidates. However, little is known about the genetic variability of genes encoding membrane proteins of Anaplasma marginale isolates. The aim of this study was to determine the degree of conservation of the genes encoding the VirB9, VirB10 proteins, conjugal transfer protein (CTP) and the elongation factor ( Ef-Tu) in the nucleotide sequence of a Cuban Anaplasma marginale isolate and from the deduced amino acid sequence and compare them with those described for Florida and Saint Maries Anaplasma marginale isolates and Anaplasma (centrale) marginale from Israel . The gene and deduced amino acid sequences showed high degree of identity with the sequences described for other isolates of Anaplasma marginale.
[Show abstract][Hide abstract] ABSTRACT: Nos últimos anos infecção por protozoários hemosporídeos dos gêneros Plasmodium e Haemoproteus, tem sido considerada um dos fatores mais importantes relacionados com a extinção e / ou declínio da população de várias espécies de aves em todo o mundo. No Brasil, apesar da grande biodiversidade aviária, poucos estudos foram desenvolvidos para detectar a infecção, especialmente entre as aves silvestres mantidas em cativeiro. Assim, o objetivo deste estudo foi analisar a prevalência de infecção por Plasmodium spp. e Haemoproteus spp. em aves silvestres em cativeiro na Mata Atlântica do sudeste do Brasil, utilizando microscopia convencional e reação em cadeia da polimerase. Amostras de sangue de 119 aves mantidas em cativeiro no Ibama durante o período de julho de 2011 a julho de 2012, foram coletadas. A densidade parasitária foi determinada com base apenas em leituras de esfregaços de sangue por microscopia fotônica. A prevalência média de infecção por Plasmodium spp. e Haemoproteus spp. obtida por exame microscópico de esfregaços sanguíneos e PCR foi semelhante (83,19% e 81,3%, respectivamente), com Caracara plancus e Saltator similis sendo as espécies mais parasitadas. A parasitemia média determinada pela contagem microscópica de formas evolutivas de Plasmodium spp. e Haemoproteus spp. foi de 1,51%. Os resultados obtidos neste estudo reforçam a importância do manejo de aves em cativeiro, especialmente quando serão reintroduzidas na natureza.
Full-text · Article · Jan 2015 · Pesquisa Veterinária Brasileira
[Show abstract][Hide abstract] ABSTRACT: Bartonellaceae is a family within the order Rhizobiales in the sub-class of Alphaproteobacteria which contains only the genus Bartonella. They are fastidious, facultative intracellular, slow-growing, gram-negative, slightly curved bacilli that cause a wide range of diseases in humans and animals, some species being zoonotic. Clinical manifestations in humans include Carrión's disease, trench fever, cat scratch disease, bacteremia with fever, bacillary angiomatosis and peliosis, endocarditis, and neuroretinitis. Colony morphology varies from clear to smooth white and rough tan. Several species embed in the agar. They are typically inert biochemically; the organisms are usually catalase and oxidase negative and aerobic, and they do not produce acid from carbohydrates. Some species are motile. The genus Bartonella, to date, contains 29 species and 3 subspecies. Most Bartonella species have successfully adapted to survival in specific domestic or wild mammals. Blood-sucking arthropod vectors are often involved. Clinical presentation generally depends on both the infecting Bartonella species and the immune status of the infected individual. Bartonella species are susceptible to many antibiotics in vitro; however, in vivo antibiotic susceptibilities do not correlate well for a number of antibiotics.
[Show abstract][Hide abstract] ABSTRACT: Anaplasma phagocytophilum is an emerging pathogen of humans, dogs and other animals, and it is transmitted by ixodid ticks. The objective of the current study was a) detect A. phagocytophilum in dogs and ixodid ticks using real-time Polymerase Chain Reaction (qPCR); and b) Determine important variables associated to host, environment and potential tick vectors that are related to the presence of A. phagocytophilum in dogs domiciled in Rio de Janeiro, Brazil.
We tested blood samples from 398 dogs and samples from 235 ticks, including 194 Rhipicephalus sanguineus sensu lato, 15 Amblyomma cajennense, 8 Amblyomma ovale and 18 pools of Amblyomma sp. nymphs. A semi-structured questionnaire was applied by interviewing each dog owner. Deoxyribonucleic acid obtained from ticks and dog buffy coat samples were amplified by qPCR (msp2 gene). The sequencing of 16S rRNA and groESL heat shock operon genes and a phylogenetic analysis was performed. The multiple logistic regression model was created as a function of testing positive dogs for A. phagocytophilum.
Among the 398 blood samples from dogs, 6.03% were positive for A. phagocytophilum. Anaplasma phagocytophilum was detected in one A. cajennense female tick and in five R. sanguineus sensu lato ticks (four males and one female). The partial sequences of the 16S rRNA, and groESL genes obtained were highly similar to strains of A. phagocytophilum isolated from wild birds from Brazil and human pathogenic strains. The tick species collected in positive dogs were R. sanguineus sensu lato and A. cajennense, with A.cajennense being predominant. Tick infestation history (OR = 2.86, CI = 1.98-14.87), dog size (OR = 2.41, IC: 1.51-12.67), the access to forest areas (OR = 3:51, CI: 1.52-16.32), hygiene conditions of the environment in which the dogs lived (OR = 4.35, CI: 1.86-18.63) and Amblyomma sp. infestation (OR = 6.12; CI: 2.11-28.15) were associated with A. phagocytophilum infection in dogs.
This is the first report of A. phagocytophilum in ixodid ticks from Brazil. The detection of A. phagocitophylum in A. cajennense, an aggressive feeder on a wide variety of hosts, including humans, is considered a public health concern.
Full-text · Article · Dec 2013 · Parasites & Vectors
[Show abstract][Hide abstract] ABSTRACT: The protozoan, Theileria equi is one of the main agents transmitted by ticks to horses, causing serious barriers to equine breeding in Brazil. Its epidemiology in the country still has gaps. Thus, this study aimed to perform serological diagnosis and assess the risk factors for military use in horses located at the Municipality of Resende, in the State of Rio de Janeiro. Samples of 174 horses were tested by enzyme-linked immunosorbent assay indirect adsorption (iELISA) using partially purified T. equi antigen. The dependent variable (seroreactivity) was tested through bivariate analysis, the association as the independent variables (gender, age, racial definition, group management, breeding time at Academia Militar das Agulhas Negras (AMAN), breeding system, the equine origin, presence and degree of tick infestation). Variables with association in bivariate analysis (p≤0.25) were included in the logistic regression model. It was found that seropositivity for T. equi was 74.14%. Multivariate analysis showed that the time of creation in AMAN higher than 15 years is associated with seropositivity for T. equi (OR: 5.70, CI: 1.23 to 22.1). It can be inferred that the area is endemic, with a high prevalence of asymptomatic carriers, and enzootic stability. This evokes care and preventive measures in the introduction of horses in the herd.
Full-text · Article · Dec 2013 · Revista brasileira de medicina veterinaria
[Show abstract][Hide abstract] ABSTRACT: The Holosporaceae family (Görtz H-D, Schmidt HJ (2005) Genus Holospora. In: Garrity et al. (eds) Bergey's manual of systematic of bacteriology, vol 2, part C, 2nd edn. Springer, New York, pp 149-151) in the Rickettsiales order (Gieszczykiewicz 1939) includes a group of gram-negative bacteria without motility and obligatory symbionts. Routinely, these bacteria occupy specific compartments in their host cells, which are generally rich in metabolites (Heckmann, K. Görtz H-D (1991) Procaryotic symbionts of ciliates. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H (eds) The prokaryotes, 2nd edn. Springer, Berlin/Heidelberg/New York, pp 3865-3890; Fokin and Karpov, Endocyt Cell Res 11:81-94, 1995). Individuals of this family are mostly endosymbionts of ciliated protozoa of the genus Paramecium (Müller 1772) that parasitize, according to the species, the macronucleus and micronucleus. The family has only one consolidated genus, Holospora, and others seven genera considered to be incertae sedis in this family, including Caedibacter, Lyticum, Odyssella, Paracedibacter, Pseudocaedibacter, Pseudolyticum, and Tectibacter (Fujishima and Fujita, J Cell Sci 76:179-187, 1985). However, Lyticum was recently moved to the Midichloriaceae family (Boscaro et al., Microb Ecol 65:255-267, 2013). Among those cited, Holospora and Caedibacter are commonly studied. Thus, H. obtusa, H. caryophila, and H. curviuscula are parasitize the macronucleus of P. caudatum and P. biaurelia; H. elegans, H. undulate, and H. accuminata can be found in micronucleus of P. caudatum and P. bursaria. The specificity for the host does not occur only by the success of cell penetration, which demonstrates that the specificity for a habitat is influenced by nuclear genes (Fujishima and Fujita, J Cell Sci 76:179-187, 1985). Its distribution is still not clearly defined, but it is believed to be associated with the distribution of its host (Fokin et al., Eur J Protistol 32:19-24, 1996).
[Show abstract][Hide abstract] ABSTRACT: The objective of this study was to evaluate factors associated with infestation by Amblyomma cajennense on horses in two microregions of the state of Rio de Janeiro. Horses on 62 farms in the municipalities of the Itaguaí and Serrana microregions were evaluated between January and May 2009. The animals were examined to determine the presence of ticks and infestation level. The animals' rearing and management were assessed on each farm property using an epidemiological questionnaire. Out of the 635 horses evaluated, 41.6% were infested with A. cajennense. It was observed that farms in low-altitude regions (OR=3.69; CI: 2.3-5.8), with unsatisfactory zootechnical and sanitary management (OR=5.92; CI: 3.8-9.2) and an extensive rearing system (OR=4.25; CI: 2.1-8.5) were factors associated with tick infestation (p < 0.05) and also with cases of high infestation on horses. Use of chemical acaricides on horses was also associated with infestation (p < 0.05); the owners described different therapeutic approaches with different treatment intervals. From the present study, low altitudes, unsatisfactory management, extensive rearing and inappropriate use of acaricide products were factors associated with occurrences of A. cajennense at different infestation levels on horses in these municipalities.
Full-text · Article · Jun 2013 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to detect Theileria equi (Laveran 1901) DNA in horses and ticks using real-time PCR and to list the factors associated with infection in animals located in the Seropedica and Petropolis municipalities of the state of Rio de Janeiro. We tested blood samples from 314 horses and samples from 300 ticks, including 191 Amblyomma cajennense, 104 Dermacentor nitens, and 5 Ixodida larvae. Factors inherent to the horse, the ownership, and animal management were obtained from an epidemiological questionnaire and were evaluated in association with the presence of T. equi DNA in the animals. Among the horses in the study, 81 % (n = 253/314) presented T. equi DNA, and the animals of the Seropedica municipality had the highest infection frequency (91 %, n = 128/141, p < 0.001). The factors that had significantly different infection frequencies by chi-squared or Fisher's exact tests (p < 0.2) were included in a logistic regression model using the R programming package. Work and walking activity (odds ratio [OR] = 5.7, CI = 2.3-14.4), reproductive activity (OR = 3.8, CI = 1.3-11.5), and tick infestation (OR = 2.6, CI = 1.1-6.2) were factors that favored the presence of T. equi DNA in the animals (p < 0.05). Among the tick samples, A. cajennense and D. nitens were the identified species. The presence of T. equi DNA was observed in 9.9 % (n = 19/191) of the A. cajennense samples and 3.8 % (n = 4/104) of the D. nitens samples. A multivariate analysis revealed that the presence of A. cajennense on the animals (OR = 4.1, CI = 1.8-9.1) was associated with the presence of T. equi DNA in the horses. In the studied municipalities, activities related to work, walking, and reproduction and the presence of ticks on the horses, particularly an intense infestation of A. cajennense, are factors that lead to infection with T. equi in the horses.
Full-text · Article · Mar 2013 · Parasitology Research
[Show abstract][Hide abstract] ABSTRACT: This study evaluated factors associated with the frequency of Leishmania spp. antibodies in dogs residing in the Itaguai micro-region, State of Rio de Janeiro, Brazil. Blood samples were collected from 524 dogs. The serum samples were submitted to indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for Leishmania spp. The frequency of seropositive dogs was 28.24% (n = 148) in the micro-region, and among the three municipalities within that region, the highest frequency (p < 0.05) was observed in Seropedica (59.46%), followed by Itaguai (29.05%) and Mangaratiba (11.49%). Regarding factors associated with the host, mongrel dogs and those over the age of two presented higher frequency of antibodies to Leishmania spp. (p < 0.05). Concerning factors related to the environment and habits of the animal, dogs residing in rural areas (FR = 1.67, p = 0.0002), living outside the residence (FR = 1.42, p = 0.0197), with access to forest, streams and pastures (FR = 2.81, p = 0.0007), remaining loose (FR = 1.66, p = 0.0073), and those that had no shelter (FR = 2.16, p < 0.0001) were more likely to be seropositive. Canine leishmaniasis is a disease with high occurrence in the Itaguai micro-region, and aspects such as the definition of breed, age, habits and care by owners showed significant association in this micro-region.
Full-text · Article · Mar 2013 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: Rev. Bras. Med. Vet., 35(1):63-68, jan/mar 2013 63 RESUMO. A babesiose canina é uma hemoparasitose causada por protozoários do gênero Babesia spp., mundialmente distribuída, e transmitida por carrapatos Ixodidae. O presente trabalho objetivou determinar os aspectos clínico-hematológicos de cães do município de Seropédica, RJ, com babesio-se diagnosticada pela PCR em tempo real e RFLP. Foram coletadas 311 amostras sanguíneas de cães.. & Faccini J.L.H. [Clinical-hematological changes due to Babesia canis vogeli infection in dogs from Seropédica, Rio de Janeiro, Brazil]. Alterações clínico-hematológicas da infecção por Babesia canis vogeli em cães do município de Seropédica, Estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veteriná-ria, 35(1):63-68, 2013. Programa de Pós-Graduação em Ciências Veterinárias, The Canine babesiosis is a disease caused by hemoparasite protozoa of the genus Babesia spp., distributed worldwide, and transmitted by Ixodidae ticks. This study aimed to determine the clinical and hematological aspects in dogs from Seropédica, RJ, with babesiosis diagnosed by real time PCR and RFLP. Were collected blood samples of 311 dogs. The diagnosis revealed the frequency of 11.90% (n=37/311) and the subs-pecies was identified as Babesia canis vogeli. The clinical parameters showed no association in the infection. As for hematological parameters were observed normocytic normochromic anemia, hemoglobinemia and thrombocytopenia (P<0.05), being signs common in babesiosis caused by B. c. vogeli. The results showed that B. c. vogeli affects dogs of this region with considerable frequency, and should be considered as an important pathogen, which is underdiagnosed, mainly due to complications in coinfec-tions and for clinical signs common to other diseases.
Full-text · Article · Feb 2013 · Revista brasileira de medicina veterinaria
[Show abstract][Hide abstract] ABSTRACT: Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.
Full-text · Article · Jun 2012 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: Neosporosis and toxoplasmosis are parasitic diseases which can cause reproductive problems in goats and sheep. The current study aimed to determine the occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep from the districts of Amarante do Maranhão and Buritirana, Imperatriz microregion, western area of Maranhão State, northeastern Brazil, and to assess factors associated to infection by these etiologic agents. Blood samples from 110 animals (46 goats and 64 sheep) from five herds were collected, and indirect immunofluorescence assay was used for serological testing. Of 46 goat samples, 17.39% (n = 8) showed anti-N. caninum antibodies and 4.35% (n = 2) anti-T. gondii, while of 64 sheep samples 4.69% (n = 3) and 18.75% (n = 12) showed anti-N. caninum and anti-T. gondii antibodies, respectively. No significant difference regarding the presence of domestic cats and/or dogs on the property and veterinary care was seen for both etiologic agents studied. However, food supplementation and animal reproductive failure were significantly (p < 0.05) for N. caninum among sheep and goats, respectively. The current study showed that goats and sheep in western Maranhão are exposed to N. caninum and T. gondii. It is the first evidence of these agents in small ruminants in this region.
Full-text · Article · Oct 2011 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: The current study aimed to investigate the seroprevalence of IgG antibodies to Anaplasma marginale in cattle from Maputo, Gaza and Inhambane provinces, south Mozambique. A total of 809 serum samples from cattle were obtained and tested by indirect enzyme-linked immunosorbent assay (i-ELISA). The chi-square test at 5% significance was used to assess the association between seroprevalence and the variables gender, age and geographic origin of animals. The overall seropositivity was 76.5% (n = 619) and anti-A. marginale antibodies were detected in 89.1% (n = 156), 68.4% (n = 308) and 84.2% (n = 155) of the animals in the provinces of Maputo, Gaza and Inhambane, respectively. A significant association (p < 0.05) was found with the geographic origin of the animals, while sex had no significant relationship. The frequencies of seropositive in the age groups were 63.2% (n = 72), 80.0% (n = 92), 83.1% (n = 98) and 77.3% (n = 357) for animals <12; >12 and ≤24; >24 and ≤36; >36 months, respectively. These results indicate that in southern Mozambique there are areas of enzootic stability to A. marginale. Thus, epidemiological monitoring is required to monitor the immune status of animals in the region.
Full-text · Article · Oct 2011 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: Anaplasma phagocytophilum was detected in dogs from Brazil in the municipalities of Seropédica and Itaguaí, Rio de Janeiro state, by real-time polymerase chain reaction (PCR) using SYBR Green to detect the amplification. Of 253 samples, 18 (7.11%) were positive, with a threshold cycle (Ct) ranging from 31 to 35 cycles. The PCR product from a positive sample was cloned and sequenced. The sequence obtained demonstrated 100% identity with other A. phagocytophilum sequences published in the GenBank database. The analytical sensitivity of RT-PCR using SYBR Green system was able to detect 3 plasmid copies when defined numbers of plasmid copies containing 122 base pairs from the msp2 gene were used. The assay was considered specific when DNA from bacteria (Anaplasma platys, Anaplasma marginale, Ehrlichia canis, Neorickettsia risticii, Rickettsia rickettsii) closely related to A. phagocytophilum was placed in the reaction. These results demonstrate that the canine granulocytic anaplasmosis agent is present in regions in which dogs could be a source of infection for tick vectors. The current study reports the detection of A. phagocytophilum, a zoonotic agent responsible for Human granulocytic anaplasmosis, in Brazilian dogs.
Full-text · Article · Jul 2011 · Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
[Show abstract][Hide abstract] ABSTRACT: Serum samples from 714 equids of Itaguaí and Serrana microregions, Rio de Janeiro, southeastern Brazil, were examined by indirect fluorescent antibody test (titer 1:80) for Theileria equi. The prevalence in the microregions and factors associated with seropositivity were evaluated and the prevalence ratio (PR) calculated. The overall prevalence of T. equi infection was 81.09% (n = 579), with higher prevalence (p < 0.05) in the Itaguaí (85.43%) when compared to Serrana microregion (76.92%). The geographic area, altitude, farming condition and area of origin of equids were associated (p < 0.05) with seropositivity for T. equi. Equids reared in the Itaguaí microregion (PR = 1.11, p = 0.003) and at altitudes below 500 m (PR = 1.10; p = 0,014) were more likely to be seropositive for T. equi. Furthermore, when equids were born in the farm (PR = 1.10, p = 0.008) and reared with poor farming conditions (PR = 1.13, p = 0.018) they were more likely to be exposed to T. equi. The main ticks found on equids were Amblyomma cajennense and Dermacentor (Anocentor) nitens. The microregions studied are endemic areas for equine theileriosis and there exists enzootic stability for T. equi. Only factors related to the collection area of serum samples influenced the seropositivity of equids for T. equi in that region.
Preview · Article · Jul 2011 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
[Show abstract][Hide abstract] ABSTRACT: The life cycle of Argas (Persicargas) miniatus in adult stage was studied in controlled conditions (27±1°C and 80±10% RH) and uncontrolled temperature and humidity conditions (room conditions during wet and dry season). Males originated preferentially from N2 and females from N3. Females in controlled conditions laid eggs up to 18 times, while females in uncontrolled room conditions, during wet and dry seasons, laid eggs 9 to 12 times, respectively. In controlled conditions, females laid 1350 eggs, whereas in uncontrolled conditions females laid 443 eggs during the wet season and 894 eggs during the dry season. Male ticks survived without feeding for 165 days in controlled conditions and 135 days in uncontrolled conditions, while females survived for 300 days in controlled conditions and 240 days in uncontrolled conditions. In ambient conditions, biological parameters of Argas (Persicargas) miniatus adult stage were negatively affected especially during the wet season when compared with adult stage kept in controlled condition.