Behnaz Sheikholslami

Tarbiat Modares University, Teheran, Tehrān, Iran

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Publications (1)1.72 Total impact

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    ABSTRACT: The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured. The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages. LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.
    Preview · Article · Jan 2008 · Journal of Assisted Reproduction and Genetics

Publication Stats

1 Citation
1.72 Total Impact Points


  • 2008
    • Tarbiat Modares University
      • Department of Anatomy
      Teheran, Tehrān, Iran