Zheng-Tao Yang

Jilin University, Yung-chi, Jilin Sheng, China

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Publications (3)4.5 Total impact

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    ABSTRACT: A trichomonad-like parasite isolated from canine fecal samples in Changchun, China was successfully cultivated in vitro using RPMI1640 medium supplemented with 10 % heat-inactivated calf serum and antibiotics. These were then subjected to scanning and transmission electron microscopy for ultrastructural study. This parasite has four anterior flagella of unequal length, one independent flagellum, and one recurrent flagellum. It exhibits an anterior nucleus, a Golgi complex, an axostyle, food vacuoles, and hydrogenosomes. These features are consistent with the ultrastructural characteristics of previously described Pentatrichomonas hominis. Polymerase chain reaction and sequence analysis of three genetic loci, including ITS1-5.8S rRNA-ITS2, 18S rRNA, and EF-1α, were also used to compare these samples with other trichomonad species. Molecular identification was also consistent with P. hominis. This is the first time that isolation of P. hominis has been isolated from dog in China, although several other strains of P. hominis have been isolated from human samples.
    No preview · Article · Mar 2014 · Parasitology Research
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    ABSTRACT: The prevalence of clinical endometritis in dairy cows was surveyed in Northeast China between March 2012 and August 2012. A total of 1370 apparently healthy dairy cows were detected from three provinces (Heilongjiang, Jilin and Liaoning) that locate in Northeast China. All uterine swabs and samples of vaginal mucus were collected and all of them were microbiologic ally analyzed. The results showed that the overall prevalence of clinical endometritis in dairy cows was 17.4% and the prevalence is similar in three different province which Heilongjiang (16.7%, 90/540), Jilin (17.6%, 74/420) and Liaoning (18.3%, 75/410). The prevalence in intensively reared dairy cows (11.9%, 107/900) was lower than that in semi-intensively reared dairy cows (28.1%, 132/470) (p<0.05). The dominant bacterial isolates in the study were Staphylococcus aureus (21.8%), Streptococcus sp. (19.2%) and Arcanobacterium pyogenes (15.0%). The results of this survey indicated the presence of clinical endometritis in dairy cows in Northeast China and rearing system is an important factor to affect the clinical endometritis in dairy cows in Northeast China and Staphylococcus species was major pathogen.
    No preview · Article · Jan 2013 · Asian Journal of Animal and Veterinary Advances
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    ABSTRACT: Selection of potent cytokine adjuvants is important for the development of Staphylococcus aureus DNA vaccines. Several potential cytokines have been proven to induce enhanced immune responses in animal models and clinical tests. There is still no reported use of IL18 as an adjuvant to design DNA vaccines against S. aureus. In this study, we cloned the main fibronectin binding protein gene (a fragment from clumping factor A, ClfA(221-550)) of S. aureus and bovine interleukin 18 (bIL18). Then recombinant plasmids were constructed based on the eukaryotic expression vector pVAX1 with or without bIL18. Indirect immunofluorescence assays in transfected HeLa cells indicated that the recombinant DNAs (rDNAs) could be expressed correctly and had antigenicity. BALB/c mice were used as experimental models to examine the immunogenicity of rDNAs in vivo. The ClfA(221-550) rDNA provoked antibody production. The bIL18 rDNA induced production of the Th1 type cytokines IL2 and IFNgamma, and ClfA(221-550) and bIL18 synergistically stimulated T-lymphocyte proliferation. The data demonstrated that bIL18 is a potent adjuvant that could be used to enhance cellular immunity.
    No preview · Article · Jun 2009 · Veterinary Immunology and Immunopathology