Zabihollah Khaksar

Shiraz University, Chimaz, Fārs, Iran

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Publications (22)5.08 Total impact

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    ABSTRACT: In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce sufficient insulin, so blood glucose increases in the mother and then in the fetus, which may cause several disturbances in neonates. This study was conducted to evaluate the effect of maternal diabetes on fetal testicular structure. Sixty adult female rats were divided into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 14–20 of pregnancy and the day of delivery the male fetuses or neonates were chosen, and their fetal gonads or neonatal testes were isolated. The number of spermatogonia cells was measured using routine histological techniques. In some samples of neonatal testes, various cellular parameters were determined using transmission electron microscopy (TEM) technique. Results revealed a significant increase in the number of germ cells in fetal male gonads and spermatogonia cells in the neonates’ testes of diabetic mothers (ND) compared to those of control. Results of TEM revealed only a few differences in the Leydig cells in testes of ND compared to that of control. Mitochondrial abnormalities were seen as mitochondrial cristea and were destroyed, vacuolation occurred in mitochondria, and the nuclei of Leydig cells were dark and condensed. In addition, the density of spermatogonia cells increased in ND compared with the control group. It is generally concluded that maternal hyperglycemia affects fetal male gonads, illustrated by an increase in the spermatogonia cells and alteration in the Leydig cells mitochondria and nuclei in neonatal testes.
    No preview · Article · Nov 2013 · Comparative Clinical Pathology
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    ABSTRACT: Purpose Maternal diabetes leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Methods Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. Thirty days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological and cellular parameters were determined using histological and electron microscopy techniques. Results Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of the controls. The weight, volume and diameter of the ovary and the ovarian capsule thickness were inclined to decrease in ODM compared to that of controls. The number and diameter of primary, pre-antral and antral follicles were decreased in ovaries in the ODM. The electro-micrographs have demonstrated the organelle alterations in oocytes and granulosa cells that suggest the apoptosis progress and oxidative stress. Conclusions Maternal hyperglycaemia exhibited deleterious effects on the female reproductive system in the offspring.
    No preview · Article · Oct 2013 · Reproductive Medicine and Biology
  • Hooman Hematian · Zabihollah Khaksar · Gholamali Jelodar
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    ABSTRACT: This study was conducted to evaluate effects of maternal diabetes on neural tube structure of rat fetuses. Twenty four adult female rats were divided into two equal groups. Diabetes was induced in one group by alloxan agent, the other group was considered as the control group. Both groups became pregnant by natural mating. At days 11 to 16 of pregnancy, the neural tube was collected from fetuses of both groups, and the weight and number of fetuses were measured. Various histological parameters were determined using histological techniques. Results revealed a decrease in transverse diameter, the length of the neural tube and the number of cells in the neural tube, and an increase in the vertical diameter of the neural tube in fetuses of diabetic mothers (FDM) compared to that of control. The body weight of FDM was significantly more than that of control, whereas the number of fetuses was significantly less than that of control. Maternal hyperglycemia exhibited deleterious effects on the central nervous system during fetal life, which affected structure, length, and cell number of the neural tube.
    No preview · Article · Sep 2013 · Comparative Clinical Pathology
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    ABSTRACT: Maternal diabetes leads to increased blood glucose concentration in the mother and consequently in the fetus, causing various neonatal problems. This study was conducted to evaluate the effect of maternal diabetes on fetal ovarian structure. Forty adult female rats were divided into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 14 to 20 of pregnancy and day 30, day 60, and day 90 after birth, the fetuses and neonates were collected from both groups and the female genital system was isolated from them. The numbers of germ cells were measured using routine histological techniques in fetuses. In the neonatal ovarian samples, various cellular parameters were determined using transmission electron microscopy (TEM) technique. Results revealed a significant decrease in the number of germ cells in the fetal female gonads in the gonads of fetuses from diabetic mothers compared to those of control. Results of TEM revealed some differences in the luteal cells and oocytes of primordial follicles in ovaries of neonates from diabetic mothers compared to that of control. Mitochondrial abnormalities were seen as mitochondrial cristae were destroyed and vacuolation occurred in the mitochondria and the nuclei of luteal cells were darken and condensed. It is generally concluded that maternal hyperglycemia affects fetal female gonads illustrated by a decrease in the oocytes and alteration in the mitochondria and nuclei of oocytes and luteal cells in neonatal ovaries.
    No preview · Article · Sep 2013 · Comparative Clinical Pathology
  • Hooman Hematian · Zabihollah Khaksar · Gholamali Jelodar
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    ABSTRACT: This study was conducted to evaluate the effects of maternal diabetes on the foetal spinal cord. Sixteen adult female rats were divided into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 17, 18, 19 and 20 of pregnancy, the spinal cord was collected from the foetuses of all rats, and the body mass and number of foetuses were also measured. Various histological parameters were determined using routine histological techniques. The results revealed a decrease in the transverse diameter of the spinal cord, the transverse diameter of central canal, the number of cells in the white and gray matters and an increase in the vertical diameter of the spinal cord and the vertical diameter of the central canal in the foetuses of diabetic mothers (FDM) as compared to the control group. The body mass of FDM was signifi cantly (P<0.05) higher than that of the control and the number of foetuses in FDM was signifi cantly (P<0.05) lower than the control. Maternal hyperglycaemia exhibited deleterious effects on the spinal cord during foetal life, which affected the shape, structure and cell number of the spinal cord. Key words: maternal diabetes, rat, foetus, alloxan, spinal cord
    No preview · Article · Jul 2013 · Veterinarski Arhiv
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    Mehrzad Jafari Barmak · Zabihollah Khaksar · Asghar Sharifi · Reza Mahmoudi
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    ABSTRACT: Ovary is a part of the reproductive system can be affected by diabetes. Aloe vera is a plant that reduces hypoglycemic. Thus, in present study, its aloe vera effect on ovaries development in during gestational age of 18 day embryo of rat. Material and methods: Two Groups of female Sprag dawlly rats were injected by streptozotocin (50 mg/kg/IP) and mating by male rat, vaginal plaque is objected. One groups of rats received aloe vera extract (400mg/kg) by oral in gestational age during and other group giving distilled water. After 18 day, rats sacrificed and their embryo were removed. Abdominal sections of embryo were processed and embedding by paraffin. The 5-6 µ sections were made and staining by hematoxyline-eosin and mason trichorome. Cellular of ovary change is evaluated. Results: Mean body weight do not show significantly (p>0.05). Mean blood sugar were decreased significantly (p<0.05). The mean of primordial germ cells were seen significant (p<0.05). Discussion: This study showed that Aloe Vera can reduce the blood sugar, also cause be increased primordial germ cells in embryos of diabetic mothers. [Mehrzad Jafari Barmak, Zabihollah Khaksar, Asghar Sharifi, Reza Mahmoudi. Effect of Aloe vera extract on ovaries development in during gestational age of 18 day embryo of diabetic rats. Life Sci J 2013;10(2s):323-327] (ISSN:1097-8135). http://www.lifesciencesite.com. 55
    Full-text · Article · Jan 2013 · Life Science Journal
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    ABSTRACT: Purpose: In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough insulin, which leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Methods: Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. 60 days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological parameters were determined using histological techniques. Results: Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of controls. The weight, volume and diameter of the ovary and ovarian capsule thickness were decreased in the ODM group. The number and diameter of primary, pre-antral, antral and preovulatory follicles and corpora lutea were decreased in ovaries in the ODM. Conclusion: Maternal hyperglycaemia exhibited deleterious effects on the reproductive system of their offspring.
    No preview · Article · Jan 2013
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    ABSTRACT: In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough insulin, which leads to increase blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Sixteen adult female rats were allocated in two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural matting. Ninety days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological parameters were determined using histological techniques. Results revealed a significant increase in body weight and blood glucose in the offspring of diabetic mothers (ODM) compared to that of controls. The weight, volume and diameter of the ovary and ovarian capsule thickness were decreased in the ODM group. The number and diameter of primary, preantral, antral and preovulatory follicles and corpora lutea were decreased in ovaries in the ODM. Maternal hyperglycaemia exhibited deleterious effects on the reproductive system of their offspring
    No preview · Article · Mar 2012 · Comparative Clinical Pathology
  • Zabihollah Khaksar · Gholamali Jelodar · Hooman Hematian
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    ABSTRACT: This study was conducted to evaluate the cytological effects of maternal diabetes in the central nervous system (CNS). Sixteen adult female rats were divided in two groups and diabetes was induced in one group using alloxan. Both groups became pregnant by natural mating. At day 7 after birth, the cerebrum, cerebellum and spinal cord were collected from offspring of all rats. The weight of the neonates and their blood glucose were measured. Various cellular parameters were determined using transmission electron microscopy. Results revealed changes in the neurons of the grey matter of the cerebrum, cerebellum and spinal cord in the offspring of diabetic mothers (ODM) compared to controls. Mitochondrial abnormalities were also detected; mitochondrial cristae were destroyed and the mitochondria showed signs of vacuolation whilst increased heterochromatin was also noted. The density of neurons and neuroglial cells in the cerebrum, cerebellum and spinal cord was decreased in ODM compared with the control group. The body weight and blood glucose of ODM were significantly higher than that of controls (p
    No preview · Article · Dec 2011 · Comparative Clinical Pathology
  • S. Gholami · S. R. Ghazi · Z. Khaksar
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    ABSTRACT: The vertebral level of termination of the spinal cord was determined during postnatal life of male camel. Observations showed that termination of the spinal cord varies with the age of animal. In new born animals upto one month age, the spinal cord terminated at third sacral vertebra, while in one year old, mostly within middle and caudal third of second sacral vertebra and rarely within cranial third of third sacral vertebra. In 2.5-3 years old animal, the termination of spinal cord was at the level of middle third to caudal third of second sacral vertebra and then with the advancement of age at 4-6 years, it was terminated in middle third to cranial third of second sacral vertebra and beyond the age of 7 years upto 10 years mostly in cranial third of second vertebra. Therefore, it can be concluded that, the conus medullaris was preceded from caudal third of third sacral vertebra at neonate while in adult stage to cranial third of second sacral vertebra.
    No preview · Article · Nov 2011 · Journal of Applied Animal Research
  • Zabihollah Khaksar · Gholamali Jelodar · Hooman Hematian
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    ABSTRACT: This study was conducted to evaluate effects of maternal diabetes on fetal brain structure especially in cerebrum. Sixteen adult female rats were divided in two groups. Diabetes was induced in one group by alloxan agent. Both groups became pregnant by natural matting. At 7, 14, 21, and 28 days after birth, the cerebrum was collected from offspring of all rats and the weight of neonates was measured. Various histological parameters were determined using histological techniques. Results revealed a significant decrease in number of cells in gray matter and white matter in all days after birth and a significant decrease in thickness of gray matter at 7 and 14 days postneonatal in cerebrum of offspring of diabetic mothers (ODM) as compared with control group. The body weight of ODM was significantly more than that of control (p< 0.05). Maternal hyperglycemia exhibited deleterious effects on brain specially cerebrum during fetal life, which remained persistent during postneonatal period.
    No preview · Article · Oct 2011 · Comparative Clinical Pathology
  • Mohammad Reza Ebrahimpoor · Zabihollah Khaksar · Ali Noorafshan
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    ABSTRACT: Anti-diabetic effect of the alcoholic extract of Otostegia persica was investigated on 40 streptozotocin diabetic rats in four equal groups. Animals in the first group did not receive any extract and acted as a control for the other groups. Rats in group II, III, and IV received a daily oral dose of O. persica extract at 200, 350, and 500mg/kg, respectively, for 3weeks. Blood glucose level was estimated three times a day (8:00 and 11:00 am and 5:30 pm) in all groups. Statistical analysis of the results by t test showed that the extract produced a dose-dependent decrease in the blood glucose level especially in the fourth group (p < 0.0001) in comparison the control group. Thus, study indicates that O. persica has a strong anti-diabetic action and can decrease blood glucose levels. KeywordsAnti-diabetic effect– Otostegia persica –Streptozotocin–Blood glucose level
    No preview · Article · Oct 2011 · Comparative Clinical Pathology
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    Paria Parto · Zabihollah Khaksar · Abbas Akramifard · Bahman Moghisi
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    ABSTRACT: In the sexually active bird the oviduct is a tortuous tube extending from the single ovary to the cloaca and occupying a large part of the abdominal cavity. Distinctive structural variation at different levels allows its subdivision into five principle parts as infundibulum, magnum, isthmus, shell gland and vagina. The morphologic characteristics of the oviduct in 8 mature laying turkey was studied by light and scanning electron microscopies. Result revealed that the mucosal membrane of the oviduct possesses the complex luminal folds covered by ciliated columnar epithelium with secretory cells. The infindibulum presented a cranial opening with thin and long fimbriae. The luminal side showed distinct longitudinal primary and secondary folds. In the magnum, the folds were thicker and filled with tubular glands.Is luminal side had longitudinal broad folds. The isthmus is short and had fewer folds. The isthmal folds were less voluminous than in the magnum. A bag shaped uterus showed thin folds. In the luminal side primary folds with discrete secondary folds were seen. The vagina had long transverse luminal folds with no gland and thick muscular tunic. In conclusion the oviduct in laying turkey has morphological similarities with the other species of birds' expect in some few points.
    Full-text · Article · Jan 2011
  • Zabihollah Khaksar · Gholamali A. Jelodar · Hooman Hematian
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    ABSTRACT: In pregnant mothers maternal diabetes happens when the pancreas cannot produce enough insulin, so blood glucose increases in the mother and then in the fetus, resulting in several injuries in neonates. This study was conducted to evaluate the effects of maternal diabetes on fetal cerebrum. Sixteen adult female rats were divided in two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 17, 18, 19 and 20 of pregnancy, the cerebrum was collected from the fetuses of all rats, also the body weight and number of fetuses was measured. Various histological parameters were determined using routine histological techniques. Results revealed a significant decrease in the ratio of gray matter to white matter and also the number of cells in gray matter and white matter in all days. There was also a significant decrease in thickness of gray matter at day 20 of pregnancy in the cerebrum of fetuses of diabetic mothers (FDM) as compared with the control group. The body weight of FDM was significantly (P<0.05) more than that of the control group and the number of fetuses in FDM was significantly (P<0.05) less than the control group. Maternal hyperglycemia exhibited deleterious effects on cerebrum during fetal life, which affected: cell number, gray matter to white matter ratio and thickness of gray and white matter. Key words: Maternal diabetes, Rat, fetus, Alloxan, Cerebrum
    No preview · Article · Jan 2011 · Iranian Journal of Veterinary Research
  • Z Khaksar · G Jelodar · H Hematian
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    ABSTRACT: Introduction: In pregnant mothers, maternal diabetes occurs when pancreas can't produce enough insulin resulting in increased blood glucose levels in the mother and subsequently in the fetus. This investigation was conducted to evaluate the effects of maternal diabetes on cerebellum of offspring of diabetic mothers (ODM), which was carried out at the veterinary faculty of Shiraz University in 2007-2008. Methods: This was an experimental study that included sixteen normal adult female rats divided in two groups. Diabetes was induced in one group by Alloxan agent. Both groups became pregnant by natural mating . At 7, 14, 21 and 28 days after birth, the cerebellum of all offsprings were collected and the weight of neonates was also measured. After producing histological slides, Olympus BX51 microscope and ‍‍‍‍‍‍‍ Olysia softwarwere used. Various histological parameters used included gray and white matters thicknesses (µ), the number of cells in gray and white matter separately per unit and the ratio of gray matter to white matter. Results: Cerebellar parameters decreased in ODM as compared to the control group. The body weight of ODM was significantly more than that of the control group (p< 0.05). Conclusions: Maternal hyperglycaemia exhibited deleterious effects on cerebellum during fetal life, which remained persistent during postneonatal period. Maternal diabetes also resulted in reduction of number of cells and thicknesses of both gray and white matter.
    No preview · Article · Jan 2010
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    M. R. Ebrahimpoo · Z. Khaksar · A. Noorafshan

    Preview · Article · Dec 2009
  • Gholamali Jelodar · Zabihollah Khaksar · Mohammad Pourahmadi
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    ABSTRACT: This investigation was conducted to evaluate the effects of maternal diabetes on foetal testicular structure and function and reproductive hormone levels. Sixteen adult female rats were divided in two groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. Blood was collected from 60-day-old male offspring from both groups and the level of testosterone, FSH and LH measured in their serum. At the same time, the weight and volume of testes and various histological parameters from testicular histological sections were determined. Results showed significant decrease in LH, FSH and testosterone in sera of offspring from diabetic mothers (ODM) compared with the control group. Body weight and weight and volume of testes from ODM were about 25.5%, 51.6% and 50.8% higher respectively than those of the control group (P < 0.05). Number of seminiferous tubules increased (+15%) significantly (P < 0.05), whilst thickness of testicular capsule (−16%), number of Leydig cells (−14%), number of Sertoli cells (−10%), number of spermatogonia (−38%) and diameter of seminiferous tubules (−17%) showed significantly reduced values in the ODM compared to controls (P < 0.05). In conclusion, maternal hyperglycaemia has deleterious effects on testicular parameters during foetal life which will affect reproductive endocrinology during puberty and may impact on fertility. KeywordsMaternal diabetes-Testis-Rat-Histomorphometry-Endocrine
    No preview · Article · Apr 2009 · Comparative Clinical Pathology
  • S.R. Ghazi · S. Gholami · Z. Khaksar
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    ABSTRACT: Allometric growth rate of the spinal cord in relation to the vertebral column was studied for the whole cord, and for the regional and segmental levels in 30 Iranian one-humped camels (Camelus dromedarius), divided into 6 groups comprising newborn, 1 year old, 2.5-3, 4-4.5, 5.5-6 and 7-10 year old, each group consisting of 5 animals. Observations in all the 6 groups revealed that although the growth of the vertebral column was greater than that of spinal cord, the difference in growth was not constant throughout the spine. While in thoracic region the growth rate of the spinal cord in relation to the vertebral column was positively allometric, in the cervical region it was almost isometric and in the caudal part of the spine there was a marked decline in growth of the spinal cord compared to the respective regions of the vertebral column. Craniocaudally the growth rate became drastically negatively allometric. Furthermore, there was a marked change in the allometric growth rate of the spinal cord segments in relation to the respective vertebral segments of each region.
    No preview · Article · Jun 1998 · Journal of Camel Practice and Research
  • S.R. Ghazi · S. Gholami · Z. Khaksar
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    ABSTRACT: The animals included in this study were 30 Iranian one humped male camels. These were assigned to 6 groups, each group consisted of 5 camels. The spinal cord of these animals was divided into 4 regions with respect to the angulation of spinal nerves. These regions were alternation of increase and decrease of the degree of angulation of spinal nerves with the spinal cord. The first region was from the first to fifth cervical, the second from the sixth cervical to fourth thoracic, the third region was from the fifth thoracic to first lumbar and the fourth region was from second lumbar to the fifth coccygeal. Angulation of the spinal nerves showed a converse correlation with the length of the dorsal roots.
    No preview · Article · Jun 1998 · Journal of Camel Practice and Research
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    S. Jafari · S. N. S. Gaur · Z. Khaksar
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    ABSTRACT: Jafari, S., Gaur S.N.S. and Khaksar, Z. 1996. Prevalence of Dirofilaria immitis in dogs of Fars province of Iran. J. Appl. Anim. Res., 9: 27–31.The study was carried out for one year to observe the prevalence of Dirofilaria immitis in dogs of Fars province of Iran. Out of a total of 114 dogs examined, 11(9.65%) were found positive for this infection. The native Iranian dogs showed highest (18.5%) infection. There was no difference in the susceptibility to this infection, in long and short-haired animals.
    Preview · Article · Mar 1996 · Journal of Applied Animal Research