[Show abstract][Hide abstract] ABSTRACT: The bone marrow origin of cells involved in neointimal formation after injury of the luminal surface of the vessel was confirmed
by highly sensitive nested polymerase chain reaction on isolated vascular wall cells. The model of intimal hyperplasia after
balloon angioplasty of the carotid artery in radiation bone marrow chimeras between male and female Wistar rats was used.
The Y chromosomes of rat male donors of the bone marrow for irradiated females were used as a marker of bone marrow-derived
cells. This approach demonstrated a bone marrow origin of a large fraction of α-actin-positive (smooth muscle) neointimal
No preview · Article · Jul 2008 · Russian Journal of Developmental Biology
[Show abstract][Hide abstract] ABSTRACT: Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cells from adult humans rapidly increased during long-term subculturing. Cytochemical assay showed that these cells contain neutral b-galactosidase, the marker of aging cells. Immunocytochemical assay demonstrated that most cells express p53 protein and inhibitor of the cell cycle p21WAF1.
No preview · Article · Aug 2002 · Bulletin of Experimental Biology and Medicine