Publications (2)1.22 Total impact
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ABSTRACT: To evaluate the effect of Jieze No. 1 Gel (JZ1) on the inner environment of vagina through observing its influences on vaginal levels of lactobacillus, glycogen and lactoferrin in mice. One hundred female Kunming mice were randomized into 5 groups, the blank control (BC) group, the blank gel (BG) group, and the three JZ1 treated groups. They were treated via vagina with saline, matrix gel, high (2 mg/microL), middle (1 mg/microL) and low (0.5 mg/microL) dose JZ1 respectively in volume of 20 microL, 72 h after being subcutaneously injected with physiological estradiol benzoate (E2) 2 microg. The 24 h and 72 h living lactobacillus in vaginal lavage fluid cultures were counted, the content of glycogen and the expression of lactoferrin in vaginal tissue were measured. No significant difference was found between the BC group and the three JZ1 treated groups in terms of lactobacillus-CFU, glycogen content and lactoferrin expression, and these indices detected at 24 h were not different to those detected at 72 h in the groups treated with various doses of JZ1 respectively (P > 0.05). Once application of JZ1 shows no effect on levels of lactobacillus, glycogen, and lactoferrin expression in the vagina of mice.
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ABSTRACT: To observe the clinical therapeutic effect of Jieze No. 1 ( I ) on cervicitis caused by ureaplasma urealyticum and its inhibitory effect on ureaplasma urealyticum (Uu) in vitro. A total of 393 patients suffering from cervicitis induced by ureaplasma urealyticum without other complications were randomly assigned to 3 groups, the combined treatment group: 140 patients treated with Chinese herbs Jieze No.1 by vaginal lavage, 30 min each time, once a day for 10 consecutive days and oral administration of Azithromycin, 1.0 g once every 72 h for three times; Jieze group: 115 patients were treated with Jieze No.1 alone by vaginal lavage, 30 min each time, once a day for 10 consecutive days; and the Azithromycin group: 138 patients were treated with oral administration of Azithromycin, 1.0 g once in 72 h for three times. All the patients were treated for 1 therapeutic course and condom were used for contraception during the treatment course. The Uu patients were examined again after 21 days of treatment. The therapeutic effect on cervicitis was observed. The experimental study of Jieze No. 1 on the Uu strain separated from the secretion of the urogenital tract was also observed. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the Uu were investigated. The total effective rate of the combined group was 85.3%, showing a significant difference compared with the Jieze group (67.8%) and the Azithromycin group (60.3%, both P<0.01). There was no statistical significance between the latter two groups (P>0.05). The clearing rate of Uu in the combined group was 78.4%, that of the Jieze group was 60.9% and the Azithromycin group was 47.9%. The combined group also showed a significant difference in comparison with the other two groups (all P<0.01). Especially for the drug-resistant strain, the clearing rate of Uu reached 48.1% in the combined group, 42.1% in the Jieze group, and 16.1% in the Azithromycin group, respectively. The clearing rate of Uu for the drug-resistant strain in the former two groups had significant differences in comparison with the latter (P<0.01, P<0.05), while there was no significant difference between the former two (P>0.05). The range of MIC and MBC of Jieze No. 1 to the drug-resistant strain of Uu was 15.62-250.00 mg/mL. To the non-drug-resistant MIC and MBC strain, it was 15.62-125.00 mg/mL. For the drug-resistant strain, MIC(50) was < or = 31.25 mg/mL, MBC(50) was < or = 62.50 mg/mL, MIC(90) was < or = 125.00 mg/mL and MBC(90) was 250.00 mg/mL. For the non-drug-resistant strain, MIC(50) was < or = 31.25 mg/mL, MBC50 was< or = 62.50 mg/mL, MIC(90) was< or = 62.50 mg/mL and MBC(90) was < or = 125.00 mg/mL. Jieze No.1 combined with Azithromycin can effectively treat cervicitis caused by Uu. The laboratory study confirms that Jieze No. 1 has an inhibitory effect on ureaplasma urealyticum strain. It has a remarkably effective therapeutic effects on drug-resistant strains, which is worthy of further research.
Huazhong University of Science and Technology
Wu-han-shih, Hubei, China
- Department of Integrative Traditional Chinese and Western Medicine