[Show abstract][Hide abstract] ABSTRACT: We investigated the protein binding affinity of magnetite (Fe3O4) and maghemite (gamma-Fe2O3) nanoparticles with against non-characterized protein from human lung cancer A549 cell line on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The binding ability of maghemite was 400 ng/mg. According to the SDS-PAGE results, the protein binding affinity of maghemite nanoparticles is stronger than magnetite nanoparticles. These data suggest that a protein can be detected with maghemite nanoparticles.
No preview · Article · Dec 2007 · Journal of Nanoscience and Nanotechnology
[Show abstract][Hide abstract] ABSTRACT: In order to detect DNA, we employed nano magnetite powders of Fe3O4. In XRD results, the nanoparticle was synthesized mainly in magnetite phase and also maghemite (r-Fe2O3) phase was trivial portion. The surface morphology of the powders observed that the particle size was about 40 nm and did not change as varying annealing temperature. In agarose gel electrophoresis analysis, after bonding DNA, Fe2O3 and Fe3O4 nanopaticles showed the fixed state in the range of lane 2-4. But the mixed particles were very weak reaction when compared with Fe3O4. Additionally, Fe3O4 presented the reaction of DNA in lane 5~7. From these results, we confirmed a chance of DNA detection by using the nanoparticle.