[Show abstract][Hide abstract] ABSTRACT: Helicobacter pylori (H. pylori) is a human gastric pathogen, which adheres to host cell and injects cytotoxin-associated gene A (CagA) to induce interleukin-8 (IL-8), inducible nitric oxide (iNOS) and cyclooxygenase 2 (COX-2). Noni (Morinda citrifolia) is found to possess anti-bacteria, anti-inflammation and anti-oxidation, but its effect on H. pylori infection is still unknown. Ethanol and ethyl acetate extracts of noni fruit were used in this study. The inhibitory effect on CagA and H. pylori-induced IL-8, iNOS and COX-2 were determined. The co-culture medium was collected for measuring neutrophil chemotaxis. Both extracts of noni fruit showed weak inhibition on H. pylori. Both ethanol and ethyl acetate extracts provided anti-adhesion of H. pylori to AGS cells and down-regulation on the CagA, IL-8, COX-2 and iNOS expression. Results also indicated both extracts relieved neutrophil chemotaxis. Noni fruit extracts down-regulated inflammatory responses during H.pylori infection, and the phenolic compounds play key role on anti-adhesion.
Full-text · Article · Feb 2014 · Journal of Agricultural and Food Chemistry
[Show abstract][Hide abstract] ABSTRACT: Corchorus olitorius L. is grown in Taiwan during summer. Tender leaves are crushed and washed by running water before eating. Five fractions including crude phenolic extracts (using 80 per cent aqueous acetone) of whole plant, leaf, stem, washed leaf (WL) and dried water washing material (WW) were used in this study. Linoleic acid autoxidation inhibitions on all fractions were higher than that on α-tocopherol. Except for WL and WW, other fractions also showed DPPH radical scavenging efficiency. The effect of all fractions on the regulation of inflammatory responses in lipopolysaccharide (LPS)-stimulated J774A.1 macrophage cells was investigated. All fractions diminished LPS-induced protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Nitric oxide (NO) and prostaglandin E2 (PGE(2)), downstream products, were also suppressed in dose-dependent manners, except for WL and WW. Oxidative modification and loss of leaf phenolics after kneading and washing greatly affected DPPH radical scavenging and inflammatory responses.
[Show abstract][Hide abstract] ABSTRACT: Leukopenia and immunity impairment usually occur during cancer therapy. Citronellol, an oil soluble compound derived from the geranium, has anticancer and antiinflammatory properties, as well as promoting wound healing. Ganoderma lucidum, Codonopsis pilosula and Angelicae sinensis are traditional Chinese herbs, all of which have proven immunomodulatory functions in laboratory-based research. This randomized, double-blind, placebo-controlled study examined whether the Chinese medicinal herb complex (CCMH; a mixture of citronellol and extracts of G. lucidum, C. pilosula and A. sinensis) improves the immune cell counts of cancer patients receiving chemotherapy and/or radiotherapy. A total of 105 cancer patients receiving chemotherapy or radiotherapy were enrolled. The quantities of immune cells in the blood of the subjects were determined before and after 6 weeks of cancer treatment, with either CCMH or a placebo. CCMH significantly reduced the depletion of leukocytes (14.2% compared with 28.2%) and neutrophils (11.0% compared with 29.1%). Analysis of the lymphocyte phenotype revealed that the patients receiving the placebo had reduced CD4 lymphocytes and natural killer (NK) cells than the CCMH-treated patients. Treatment with CCMH for patients receiving chemotherapy and/or radiotherapy may improve their immune function, improving their ability to fight off the cancer, as well as any secondary infections that could compromise their treatment and their health.
Full-text · Article · Jun 2009 · Phytotherapy Research