Xiangmei Ren

Nanjing Medical University, Nan-ching, Jiangsu Sheng, China

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Publications (2)2.66 Total impact

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    ABSTRACT: It has been demonstrated that many flavonoids possess a potent and broad spectrum of antitumor activity. Liquiritigenin is a flavanone extracted from Glycyrrhizae. This study investigated the effects of liquiritigenin on cell viability and apoptosis induction in human cervical carcinoma (HeLa) cells. The results show that liquiritigenin significantly suppressed cell proliferation in a dose- and time-dependent manner in HeLa cells. In addition, liquiritigenin promoted apoptosis in HeLa cells, evidenced by apoptotic morphological changes and Annexin-V binding. The apoptosis induction with liquiritigenin is associated with the up-regulation of p53 and Bax, along with down-regulation of Bcl-2 and survivin. Finally, examination of the mitochondrial pathway of apoptosis revealed that cytochrome c is released from mitochondria to cytosol, associated with the activation of caspase-9 and -3, and the cleavage of poly (ADP-ribose) polymerase (PARP). Overall, the results indicate that liquiritigenin induces apoptosis in part via the mitochondrial pathway, which is associated with p53 up-regulation, release of cytochrome c and elevated activity of caspase-9 and -3 in HeLa cells.
    No preview · Article · Nov 2010 · Phytotherapy Research
  • Yunqing Cai · Xiangmei Ren · Dongqing Xu · Mingyan Wang · Xiaoli Wu
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    ABSTRACT: To study the effect of cadmium on the apoptosis of porcine renal proximal tubule epithelial cells (LLC-PK1) and the relationships between the apoptosis and gene expression of the bcl-2 and mutant p53 (mtp53) protein. Apoptotic bodies were examined by electron microscopy, the rates of apoptosis were measured by flow cytometry (FCM) and DNA ladder was detected by DNA gel electrophoresis. The expressions of bcl-2 and mtp53 were measured respectively by FCM. The apoptotic bodies of LLC-PK1 cells treated with CdCl2 at dose of 40 micromol/L for 12 h were observed by electron microscopy and the apoptosis rates of LLC-PK1 cells were 32.61%, which were higher than those of the control group (P < 0.01). Apparent DNA ladder was showed by DNA gel electrophoresis. LLC-PK1 cells were incubated with CdCl2 at doses of 0, 10, 20, 40 micromol/L for 4 h or 8 h, it was found that expressions of bcl-2 and mtp53 were decreased and where a significant dose-response was found (r = -0.910 for bcl-2 8 h, r = -0.716, -0.972 for mtp53 4 h, 8 h, respectively). The results were suggested that the apoptosis of LLC-PK1 cells induced by cadmium might be associated with the inhibitions of the expression of bcl-2 and p53.
    No preview · Article · Dec 2004 · Wei sheng yan jiu = Journal of hygiene research

Publication Stats

22 Citations
2.66 Total Impact Points


  • 2010
    • Nanjing Medical University
      • Department of Nutrition and Food Hygiene
      Nan-ching, Jiangsu Sheng, China