Ryu-ichi KITAMURA

Gunma University, Maebashi-shi, Gunma-ken, Japan

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Publications (2)3.99 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Conophylline and betacellulin-delta4 reproduce differentiation-inducing activity of activin A and betacellulin, respectively. We examined the effect of conophylline and betacellulin-delta4 on beta cell differentiation. In AR42J cells, conophylline and betacellulin-delta4 converted them into insulin-producing cells. Cells treated with conophylline and betacellulin-delta4 continued to grow after differentiation. Thus, cell number and insulin content were much greater compared to cells treated with activin A and betacellulin. Furthermore, cells treated with conophylline and betacellulin-delta4 secreted insulin in response to glucose. Likewise, conophylline and betacellulin-delta4 converted pancreatic ductal cells into insulin-producing cells. Insulin content, cell number and glucose-evoked insulin secretion were significantly greater than those in cells treated with activin A and betacellulin. Transplantation of pseudoislets prepared using ductal cells treated with conophylline and betacellulin-delta4 was able to reduce effectively the plasma glucose concentration in streptozotocin-treated nude mice. Conophylline and betacellulin-delta4 are effective in inducing differentiation of beta cells from progenitors.
    No preview · Article · May 2007 · Endocrine Journal
  • [Show abstract] [Hide abstract]
    ABSTRACT: Conophylline and betacellulin-delta 4 reproduce differentiation-inducing activity of activin A and betacellulin, respectively. We examined the effect of conophylline and betacellulin-delta 4 on P cell differentiation. In AR42J cells, conophylline and betacellulin-delta 4 converted them into insulin-producing cells. Cells treated with conophylline and betacellulin-delta 4 continued to grow after differentiation. Thus, cell number and insulin content were much greater compared to cells treated with activin A and betacellulin. Furthermore, cells treated with conophylline and betacellulin-delta 4 secreted insulin in response to glucose. Likewise, conophylline and betacellulin-delta 4 converted pancreatic ductal cells into insulin-producing cells. Insulin content, cell number and glucose-evoked insulin secretion were significantly greater than those in cells treated with activin A and betacellulin. Transplantation of pseudoislets prepared using ductal cells treated with conophylline and betacellulin-delta 4 was able to reduce effectively the plasma glucose concentration in streptozotocin-treated nude mice. Conophylline and betacellulin-delta 4 are effective in inducing differentiation of beta cells from progenitors.
    No preview · Article · Apr 2007 · Endocrine Journal

Publication Stats

17 Citations
3.99 Total Impact Points

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Institutions

  • 2007
    • Gunma University
      • Institute for Molecular and Cellular Regulation
      Maebashi-shi, Gunma-ken, Japan