[Show abstract][Hide abstract] ABSTRACT: Aim of the study: An ethnobotanical survey on medicinal plants used in the management of sickle cell disease by traditional healers was carried out at Kisangani city and Babogombe village, two localities belonging to the Tshopo district, DR Congo in order to: (a) identify medicinal plants used by traditional healers in the management of sickle cell anemia, (b) verify their antisickling activity in vitro, (c) determine the most active plants, and (d) verify if anthocyanins (crude extracts) are responsible for the bioactivity. Materials and methods: The Emmel's test was used in vitro for the antisickling activity assays of aqueous and ethanolic extracts of different parts of these plants and for crude extracts of anthocyanins. The effect of anthocyanins on the Fe3+/Fe2+ ratio was carried out using UV-visible spectroscopy. Results: Using information from traditional healers, 18 plant species belonging to 17 genera and 12 families were collected. These were identified at the herbarium service of the Faculty of Science of the University of Kisangani. Euphorbiaceae is the predominant family (44% at Babogombe and 20% at Kisangani). Fourteen plants showed in vitro antisickling activity for both aqueous and ethanolic extracts (Alchornea cordifolia, Bridelia atroviridis, Bridelia ripicola, Carica papaya, Centella asiatica, Fagara macrophylla, Ficus mucuso, Harungana madagascariensis, Macaranga monandra, Maesopsis emini, Morinda lucida, Thomandersia hensii, Ricinodendron heudelotii, Urera hypselodendron). Two plants exhibited activity only for the aqueous extract (Acacia kirki, Mitragyna stipulosa) and two others were found to be non active (Duvernoya splendens, Eleutherine plicata). Anthocyanin crude extracts of three of these plants showed an interesting biological activity. The anthocyanin crude extracts were able to improve the Fe2+/Fe3+ ratio, facilitating the conversion of methemoglobin to hemoglobin, increasing the oxygen affinity of sickle cell hemoglobin. Conclusion: These results suggest a possible correlation between the antisickling activity of these plants and their use in management of sickle cell disease by traditional healers. This activity could be due to the anthocyanins that act as a reducing agent.
[Show abstract][Hide abstract] ABSTRACT: Antisickling activity of anthocyanins extract from a Congolese plant ( Hymenocardia acida Tul.) was evaluated using Emmel test. Chromatographic separations using chloroform-benzene (2:1) provided three fractions A<sub>1</sub>, A<sub>2</sub> and A<sub>3</sub> with the most polar [A<sub>1</sub> (TLC, R<sub>f</sub> = 0.21)] exhibiting the highest activity. Thermal kinetic degradation of this fraction at 100 and 120°C produced a first order rate constants k = 2.64x10<sup>-4</sup> and 4.08x10<sup>-4</sup>, respectively. Structural elucidation of isolated compounds is in progress.
Full-text · Article · Jan 2009 · International Journal of Pharmacology