Kangle Yi

Hunan Institute of Science and Technology, Ngo̍k-yòng-sṳ, Hunan, China

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Publications (8)8.6 Total impact

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    ABSTRACT: Nerve growth factor promotes the survival and differentiation of nervous cells and is thought to play an important role in the development of reproductive tissues. The aims of this work were to detect the presence of NGF and its receptor NTRK1 in bovine oviduct samples, and to investigate the regulatory interactions between NGF/NTRK1 and gonadotrophins in bovine oviduct epithelial cells. Both transcripts and proteins of NGF and NTRK1 were detected by RT-PCR and Western blotting, and the corresponding proteins were specifically immunolocalized in oviduct epithelial cells. In addition, real-time PCR experiments revealed that the levels of NGF and NTRK1 mRNA in oviduct epithelial cells treated with exogenous FSH or LH were greater than those in negative control cells (P < 0.05). Similarly, treatment with NGF significantly increased the expression of FSHR and LHR in oviduct epithelial cells via its effects on NTRK1 (P < 0.05). This process was suppressed by treatment with the NTRK1 inhibitor K252α. We conclude that NGF/NTRK1 may have a role in regulating the function of bovine oviducts via its interactions with gonadotrophins.
    No preview · Article · Oct 2014 · Animal Reproduction Science
  • Wei Zhang · Kangle Yi · Chao Chen · Xiaofeng Hou · Xu Zhou
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    ABSTRACT: Although cryopreserved boar semen has been available since 1975, a major breakthrough in commercial application has not yet occurred due to the high susceptibility of boar spermatozoa to damage during cryopreservation and the complicated process required for deep freezing. In recent years, the application of antioxidants during the cryopreservation of boar semen has been the subject of considerable research aimed at improving the quality of post-thaw semen. Centrifugation is necessary before using cryopreservation protocols for freezing boar spermatozoa. Studies of the effect of different centrifugation regimens on boar sperm recovery, yield and cryosurvival have made significant contributions. Therefore this review elucidates results of recent applications of various antioxidants and centrifugation regimens used in efforts to improve cryopreservation of boar spermatozoa. This review is intended to enhance understanding of the roles of these antioxidants and centrifugation regimens with respect to mechanisms that increase resistance to cryodamage of boar spermatozoa. In addition, the discussion addresses the need for developing an objective evaluation of effectiveness and estimating the prospect of application of new techniques for the cryopreservation of boar semen and its use in artificial insemination.
    No preview · Article · May 2012 · Animal reproduction science
  • Wei Zhang · Kangle Yi · Haifeng Yan · Xu Zhou
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    ABSTRACT: There have been intensive attempts to establish reliable in vitro production (IVP) and cryopreservation methods of embryos in pigs. Although a great deal of progress has been made, current IVP systems and cryopreservation still suffer from insufficient cytoplasmic abilities of in vitro matured oocytes, polyspermic fertilization, poor quality of in vitro produced embryos and low efficiency of embryo cryopreservation. Compared to other mammalian species, pig oocytes and embryos are characterized by large amounts of lipid content stored mainly in the form of lipid droplets in the cytoplasm. This fact has a negative influence on biotechnological applications on porcine oocytes and embryos. In this review, we will discuss recent studies about methods and techniques for modifying porcine embryo IVP system and embryo cryopreservation that produces high quality of pig blastocysts using in vitro maturation, in vitro fertilization, in vitro culture, microsurgical manipulation, addition of protein, the use of cytoskeleton stabilizing agents and various physical methods. The presented methods and techniques make it possible to modify the characteristics of oocytes and embryos and thus may become major tools in mammalian gamete and embryo agricultural or biotechnological applications in the future.
    No preview · Article · May 2012 · Animal reproduction science
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    ABSTRACT: The factor in the germline alpha (FIGLA) is a basic helix-loop-helix transcription factor that is essential for folliculogenesis and regulates expression of zona pellucida genes in mouse and human. But bovine homologue has not as yet been confirmed experimentally. Here, researchers present the first cDNA cloning and transcript expression analysis of the bovine FIGLA gene. Using RT-PCR and quantitative real-time PCR, researchers revealed that expression within adult cattle tissues is limited to the ovary. The researchers found that Germinal Vesicle (GV) oocytes, Metaphase II (Mil) oocytes, 4 and 8 cell embryos, morula and blastocysts were all shown to express mRNA for FIGLA and expression were different in bovine oocytes and IVF embryos at different stages (p<0.05) with the highest expression in Germinal Vesicle (GV) oocytes and lower expression in 8 cells and blastocysts embryos. There was no difference in mRNA levels for FIGLA gene among bovine IVF, PA (Parthenogenetic Activated) and NT (Nuclear Transfer) blastocysts (p>0.05). In situ hybridization the FIGLA RNA was only localized in ovarian follicle. The persistence of FIGLA in adult cowes suggests that it may regulate additional pathways that are essential for bovine ovarian and embryonic development.
    No preview · Article · Feb 2012
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    ABSTRACT: The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculated. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.
    Preview · Article · Oct 2011 · Asian Australasian Journal of Animal Sciences
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    ABSTRACT: 1. The goose major histocompatibility complex (MHC) class IIB cDNA (Ancy-MHCII) was cloned by homology cloning and rapid amplification of cDNA ends by polymerase chain reaction (RACE-PCR), and the genomic structure and tissue expression were investigated. 2. Three different 5'-RACE sequences (Ancy-MHC II5'-1, Ancy-MHC II5'-2, Ancy-MHC II5'-3), one 3'-RACE sequence (Ancy-MHC II-3') and two different full length Ancy-MHC IIB cDNA sequences (Ancy-CD01, Ancy-CD02), which came from different alleles at one locus or different loci, were determined. 3. The genomic organisation is composed of 6 exons and 5 introns, with a longer intron region than that of the chicken. The alleles encode 259 and 260 amino acids in the mature protein. 4. The number of non-synonymous substitutions (dN) in the peptide-binding region of exon 2 from 8 alleles was higher than that of the synonymous substitutions (dS). 5. Tissue-specific expression of Ancy-MHC II mRNA was detected in an adult goose using RT-PCR. These results showed that Ancy-MHC II mRNA was expressed in the lung, spleen, liver, intestine, heart, kidney, pancreas, brain, skin and muscle. This is consistent with the expression of MHC class IIB in various tissues from the chicken. 6. Sequences from goose, snipe and duck clustered together when compared with known MHC class IIB sequences from the other species, significantly differing from mammals and aquatic species, indicating a pattern consistent with accepted evolutionary pathways.
    No preview · Article · Jun 2011 · British Poultry Science
  • Lu Chen · Kangle Yi · Yanling Sun · Lina Tang · Xu Zhou
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    ABSTRACT: Neurotrophins (NTs) belong to a family of soluble homodimeric polypeptide growth factors and are widely recognized for their essential roles in central and peripheral nervous systems. One such neurotrophin, Brain-Derived Neurotrophic Factor (BDNF) was originally described in the nervous system but has now been shown to be expressed in reproductive system. In this study, the researchers examined the presence and different expression levels of BDNF mRNA in porcine reproductive organs during different stages of estrous cycle and in pig oocytes in Germinal Vesicle (GV) and in vitro matured Metaphase II (Mil) stage. In oviduct and uterus, BDNF mRNA expression was higher than that in ovary (p<0.05). The expression level in luteal phase ovary is higher than that in follicular phase ovary but the difference between them was not significant (p>0.05), a similar but more significant change occurred in oviduct (p<0.05). However, the expression levels in uterus were on an opposite trend i.e., a higher level of mRNA for BDNF was found in follicular phase uterus instead of in luteal phase uterus. BDNF mRNA was also detected in GV oocytes and in vitro matured Mil oocytes with significantly higher amounts in GV oocytes than in Mil oocytes (p<0.01). These results suggest a possible role for BDNF in the regulation and modulation of pig reproductive function and oocyte maturation.
    No preview · Article · Jan 2011 · Journal of Animal and Veterinary Advances
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    ABSTRACT: In order to understand the variations of genomic organization of the major histocompatibility complex (MHC) and provide data for the studies on disease resistance of avian species, the MHC class II polymorphism in Chinese Z-goose was investigated for the first time in the present study. Eight alleles, which probably came from different loci, were found in six different geese with only one obvious band in the restriction fragment length polymorphism data. The numbers of nonsynonymous substitutions (dN) in peptide binding region of exon 2 were higher than that of synonymous substitutions (dS), and no stop codons or frameshift mutations were found in this region, indicating that balance selection was in operation, and the sequences are not likely to be pseudogenes. In addition, we successfully obtained five different long MHC class II fragments (about 1,162 bp) in six geese and found that the length of intron 1 was longer than that in chicken and some other birds, but intron 2 seemed to be intermediate in length. The phylogenetic tree appeared to branch in an order consistent with accepted evolutionary pathway.
    Preview · Article · Jun 2009 · Immunogenetics

Publication Stats

27 Citations
8.60 Total Impact Points


  • 2012
    • Hunan Institute of Science and Technology
      Ngo̍k-yòng-sṳ, Hunan, China
  • 2011
    • Jilin Agricultural University
      Yung-chi, Jilin Sheng, China
  • 2009
    • Jilin University
      • College of Animal Science and Veterinary Medicine
      Yung-chi, Jilin Sheng, China